|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
|
|
Deletion
|
9-1
(LD)
,
DE(gpt-lac)5
,
DE(argF-lac)169
,
OCR36,37-6 (LD 9C::Ap)
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
cynS
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
cynS
Search strains by all related name:
cynS b0340 cnt ECK0337 JW0331
|
General information (Go to Linear View: )
|
|
Gene Name
|
cynS
|
|
Alternative name
|
b0340,cnt,ECK0337,JW0331
|
|
Location, Length
|
358,713 - 359,183
(
+
)
;
7.73
min
;
471
(bp) ,
156
(aa)
|
|
Product
|
cyanate aminohydrolase
|
|
Operon Name
|
cynTSX
|
|
Note
|
cyanate aminohydrolase, cyanase; GO_process: GO:0009440 - cyanate catabolic process; GO_process: GO:0006807 - nitrogen compound metabolic process; GO_process: GO:0006805 - xenobiotic metabolic process
|
|
Function
|
enzyme; Central intermediary metabolism: Pool, multipurpose conversions
|
|
Gene Ontology
|
GO:0003677
;
DNA binding ( cynS )
GO:0008152
;
metabolic process ( cynS )
GO:0008824
;
cyanate hydratase activity ( cynS )
GO:0009439
;
cyanate metabolic process ( cynS )
GO:0016829
;
lyase activity ( cynS )
GO:0016836
;
hydro-lyase activity ( cynS )
|
|
PID
|
1786535
|
EC number
(KEGG Pathway)
|
4.2.1.104
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
cynS
(
EG10175
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
CYNS_ECOLI
(
P00816
)
|
|
- Protein name
|
Cyanate hydratase
|
|
- Synonyms
|
EC 4.2.1.104, Cyanase, Cyanate lyase, Cyanate hydrolase
|
|
- Gene name
|
Name=cynS; Synonyms=cnt; OrderedLocusNames=b0340;
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Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
cynS
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Site Directed Mutagenesis Of Key Residues Involved In The Catalytic Mechanism Of Cyanase
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Cyanate hydratase
|
|
PSI-BLAST
|
Cyanate hydratase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
cyanate hydratase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
LEUCINE_ZIPPER
|
|
MMBR References
|
FEMS Microbiol Rev. 1990;7(3-4):247-52.
The cyanase operon and cyanate metabolism.
Anderson PM, Sung YC, Fuchs JA.
(
2094285
)
|
J Bacteriol. 1993;175:1443-1451
A physiological role for cyanate-induced carbonic anhydrase in Escherichia coli.
Guilloton, M. B., A. F. Lamblin, E. I. Kozliak, M. Gerami-Nejad, C. Tu, D. Silverman, P. M. Anderson, and J. A. Fuchs.
|
J Gen Microbiol. 1987;133(3):645-53.
Isolation and characterization of Escherichia coli mutants lacking inducible cyanase.
Guilloton M, Karst F.
(
3309165
)
|
J Bacteriol. 1993;175:7990-7999
Expression and purification of the cynR regulatory gene product: CynR is a DNA-binding protein.
Lamblin, A. F., and J. A. Fuchs.
|
J Bacteriol. 1987;169(6):2639-42.
Identification, mapping, and cloning of the gene encoding cyanase in Escherichia coli K-12.
Sung YC, Parsell D, Anderson PM, Fuchs JA.
(
3034861
)
|
J Bacteriol. 1992;174:3645-3650
The Escherichia coli K-12 cyn operon is positively regulated by a member of the lysR family.
Sung, Y.-C., and J. A. Fuchs.
|
J Bacteriol. 1987;169(11):5224-30.
Characterization of high-level expression and sequencing of the Escherichia coli K-12 cynS gene encoding cyanase.
Sung YC, Anderson PM, Fuchs JA.
(
2822670
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MIQSQINRNI RLDLADAILL SKAKKDLSFA EIADGTGLAE AFVTAALLGQ QALPADAARL VGAKLDLDED
0071 SILLLQMIPL RGCIDDRIPT DPTMYRFYEM LQVYGTTLKA LVHEKFGDGI ISAINFKLDV KKVADPEGGE
0141 RAVITLDGKY LPTKPF
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CCAGTTTGTG CCACTGGCCG CTAATCCTCG
-070 CGTTTGTGCC ATACCGCTAC GCCAACCGAC CGCAGCGTAA CCTTATTTTT AAACCATCAG GAGTTCCACC
0001 atgattcagt cacaaattaa ccgcaatatt cgtcttgatc ttgccgatgc cattttgctc agcaaagcta
0071 aaaaagatct ctcatttgcc gagattgccg acggcaccgg tctggcagaa gcctttgtaa ccgcggcttt
0141 gctgggtcag caggcgcttc ctgccgacgc cgcccgcctg gtcggggcga agctggatct cgacgaagac
0211 tccattctac tgttgcagat gattccactg cgtggctgca ttgatgaccg tattccaact gacccaacga
0281 tgtatcgttt ctatgaaatg ttgcaggtgt acggtacaac cctgaaagcg ttggttcatg agaaatttgg
0351 cgatggcatt attagcgcga ttaacttcaa actcgacgtt aagaaagtgg cggacccgga aggtggcgaa
0421 cgtgcggtca tcaccttaga tggtaaatat ctgccgacca aaccgttctg aCAGCCATGC GCAACCATCA
0491 AAAGACGTTC ACGATGCTGC TGGTACTGGT GCTGATTGGT CTTAATATGC GACCACTGCT CACCTCCGTC
0561 GGGCCACTGC T
|
