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PEC Original Annotations
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Essentiality
|
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Class
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non-essential
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References (PMID)
|
|
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Deletion
|
9-1
(LD)
,
DE(gpt-lac)5
,
DE(argF-lac)169
,
OCR36,37-6 (LD 9C::Ap)
(D)
|
|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
codA
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Related strains
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Strains Search
|
Search strains by gene name:
codA
Search strains by all related name:
codA b0337 ECK0334 JW0328
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General information (Go to Linear View: )
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Gene Name
|
codA
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Alternative name
|
b0337,ECK0334,JW0328
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|
Location, Length
|
355,395 - 356,678
(
+
)
;
7.66
min
;
1284
(bp) ,
427
(aa)
|
|
Product
|
cytosine/isoguanine deaminase
|
|
Operon Name
|
codBA
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Note
|
GO_component: GO:0005737 - cytoplasm; GO_process: GO:0015949 - nucleobase, nucleoside and nucleotide interconversion
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Function
|
enzyme; Salvage of nucleosides and nucleotides
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Gene Ontology
|
GO:0004131
;
cytosine deaminase activity ( codA )
GO:0005506
;
iron ion binding ( codA )
GO:0016787
;
hydrolase activity ( codA )
GO:0016810
;
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds ( codA )
GO:0019858
;
cytosine metabolic process ( codA )
GO:0046872
;
metal ion binding ( codA )
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|
PID
|
1786531
|
EC number
(KEGG Pathway)
|
3.5.4.1
|
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
|
codA
(
EG11326
)
|
|
Number of removed
|
1 aa cleaved
|
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
CODA_ECOLI
(
P25524
)
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|
- Protein name
|
Cytosine deaminase
|
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- Synonyms
|
EC 3.5.4.1, Cytosine aminohydrolase
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- Gene name
|
Name=codA; OrderedLocusNames=b0337;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
|
codA
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Bacterial Cytosine Deaminase D314s Mutant.
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|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Cytosine deaminase
|
|
PSI-BLAST
|
Urease alpha subunit
|
|
nr
(database updated :
2007.02.20
)
|
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BLAST
|
cytosine deaminase [Escherichia coli]
|
|
Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
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MMBR References
|
Mol Gen Genet. 1972;118(4):323-5.
Location of gene specifying cytosine deaminase in Escherichia coli.
Ahmad SI, Pritchard RH.
(
4570159
)
|
Mol Gen Genet. 1976;147(3):263-9.
Heptose-deficient mutants of Escherichia coli K12 deficient in up to three major outer membrane proteins.
van Alphen W, Lugtenberg B, Berendsen W.
(
787763
)
|
Mol Microbiol. 1992;6(10):1335-44.
Characterization of the Escherichia coli codBA operon encoding cytosine permease and cytosine deaminase.
Danielsen S, Kilstrup M, Barilla K, Jochimsen B, Neuhard J.
(
1640834
)
|
Arch Microbiol. 1989;152(2):115-8.
Pyrimidine, purine and nitrogen control of cytosine deaminase synthesis in Escherichia coli K 12. Involvement of the glnLG and purR genes in the regulation of codA expression.
Andersen L, Kilstrup M, Neuhard J.
(
2673119
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSNNALQTII NARLPGEEGL WQIHLQDGKI SAIDAQSGVM PITENSLDAE QGLVIPPFVE PHIHLDTTQT
0071 AGQPNWNQSG TLFEGIERWA ERKALLTHDD VKQRAWQTLK WQIANGIQHV RTHVDVSDAT LTALKAMLEV
0141 KQEVAPWIDL QIVAFPQEGI LSYPNGEALL EEALRLGADV VGAIPHFEFT REYGVESLHK TFALAQKYDR
0211 LIDVHCDEID DEQSRFVETV AALAHHEGMG ARVTASHTTA MHSYNGAYTS RLFRLLKMSG INFVANPLVN
0281 IHLQGRFDTY PKRRGITRVK EMLESGINVC FGHDDVFDPW YPLGTANMLQ VLHMGLHVCQ LMGYGQINDG
0351 LNLITHHSAR TLNLQDYGIA AGNSANLIIL PAENGFDALR RQVPVRYSVR GGKVIASTQP AQTTVYLEQP
0421 EAIDYKR
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CCGGTCAACG CGGTATTAGG TGGCGCGCTG
-070 AGCTATCTGA TCCTTAACCC GATTTTGAAT CGTAAAACGA CAGCAGCAAT GACGCATGTG GAGGCTAACA
0001 gtgtcgaata acgctttaca aacaattatt aacgcccggt taccaggcga agaggggctg tggcagattc
0071 atctgcagga cggaaaaatc agcgccattg atgcgcaatc cggcgtgatg cccataactg aaaacagcct
0141 ggatgccgaa caaggtttag ttataccgcc gtttgtggag ccacatattc acctggacac cacgcaaacc
0211 gccggacaac cgaactggaa tcagtccggc acgctgtttg aaggcattga acgctgggcc gagcgcaaag
0281 cgttattaac ccatgacgat gtgaaacaac gcgcatggca aacgctgaaa tggcagattg ccaacggcat
0351 tcagcatgtg cgtacccatg tcgatgtttc ggatgcaacg ctaactgcgc tgaaagcaat gctggaagtg
0421 aagcaggaag tcgcgccgtg gattgatctg caaatcgtcg ccttccctca ggaagggatt ttgtcgtatc
0491 ccaacggtga agcgttgctg gaagaggcgt tacgcttagg ggcagatgta gtgggggcga ttccgcattt
0561 tgaatttacc cgtgaatacg gcgtggagtc gctgcataaa accttcgccc tggcgcaaaa atacgaccgt
0631 ctcatcgacg ttcactgtga tgagatcgat gacgagcagt cgcgctttgt cgaaaccgtt gctgccctgg
0701 cgcaccatga aggcatgggc gcgcgagtca ccgccagcca caccacggca atgcactcct ataacggggc
0771 gtatacctca cgcctgttcc gcttgctgaa aatgtccggt attaactttg tcgccaaccc gctggtcaat
0841 attcatctgc aaggacgttt cgatacgtat ccaaaacgtc gcggcatcac gcgcgttaaa gagatgctgg
0911 agtccggcat taacgtctgc tttggtcacg atgatgtctt cgatccgtgg tatccgctgg gaacggcgaa
0981 tatgctgcaa gtgctgcata tggggctgca tgtttgccag ttgatgggct acgggcagat taacgatggc
1051 ctgaatttaa tcacccacca cagcgcaagg acgttgaatt tgcaggatta cggcattgcc gccggaaaca
1121 gcgccaacct gattatcctg ccggctgaaa atgggtttga tgcgctgcgc cgtcaggttc cggtacgtta
1191 ttcggtacgt ggcggcaagg tgattgccag cacacaaccg gcacaaacca ccgtatatct ggagcagcca
1261 gaagccatcg attacaaacg ttgaACGACT GGGTTACAGC GAGCTTAGTT TATGCCGGAT GCGGCGTGAA
1331 CGCCTTATCC GGCCTACGTA GAGCACTGAA CTCGTAGGCC TGATAAGCGT AGCG
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