|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Mol Microbiol. 1997;25(4):661-70.
Overinitiation of chromosome replication in the Escherichia coli dnaAcos mutant depends on activation of oriC function by the dam gene product.
Katayama T, Akimitsu N, Mizushima T, Miki T, Sekimizu K.
(
9379896
)
|
|
|
Deletion
|
OCL19,20-1
(D)
,
OCL19,20-2
(D)
,
OCL19,20-3
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
aroK
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
aroK
Search strains by all related name:
aroK b3390 ECK3377 JW5947
|
General information (Go to Linear View: )
|
|
Gene Name
|
aroK
|
|
Alternative name
|
b3390,ECK3377,JW5947
|
|
Location, Length
|
3,516,565 - 3,517,086
(
-
)
;
75.79
min
;
522
(bp) ,
173
(aa)
|
|
Product
|
shikimate kinase I
|
|
Operon Name
|
aroKB-damX-dam-rpe-gph-trpS
|
|
Note
|
GO_process: GO:0009423 - chorismate biosynthetic process
|
|
Function
|
enzyme; Amino acid biosynthesis: Chorismate
|
|
Gene Ontology
|
GO:0000166
;
nucleotide binding ( aroK )
GO:0000287
;
magnesium ion binding ( aroK )
GO:0004765
;
shikimate kinase activity ( aroK )
GO:0005524
;
ATP binding ( aroK )
GO:0005737
;
cytoplasm ( aroK )
GO:0008652
;
amino acid biosynthetic process ( aroK )
GO:0009073
;
aromatic amino acid family biosynthetic process ( aroK )
GO:0016301
;
kinase activity ( aroK )
GO:0016740
;
transferase activity ( aroK )
GO:0046872
;
metal ion binding ( aroK )
|
|
PID
|
87082255
|
EC number
(KEGG Pathway)
|
2.7.1.71
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
aroK
(
EG10081
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show details
)
|
Entry name(Acc.no)
|
AROK_ECOLI
(
P24167
;
P78113; Q8X4S0
)
|
|
- Protein name
|
Shikimate kinase I
|
|
- Synonyms
|
EC 2.7.1.71, SKI
|
|
- Gene name
|
OrderedLocusNames=b3390, c4160, z4743, ECs4232, SF3408, S4354;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
aroK
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of The Escherichia Coli Shikimate Kinase I (Arok)
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Shikimate kinase 1 (SK 1) (Shikimate kinase I) (SKI)
|
|
PSI-BLAST
|
Shikimate kinase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
shikimate kinase I [Escherichia coli 536]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
ATP_GTP_A
SHIKIMATE_KINASE
|
|
MMBR References
|
DNA Seq. 1995;5(3):195-7.
The gene (aroK) encoding shikimate kinase I from Escherichia coli.
Griffin HG, Gasson MJ.
(
7612934
)
|
Mol Microbiol. 1992;6(13):1841-51.
Expression of the Escherichia coli dam gene.
Lobner-Olesen A, Boye E, Marinus MG.
(
1630320
)
|
J Bacteriol. 1992;174:525-529
Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli.
Lobner-Olesen, A., and M. G. Marinus.
|
J Bacteriol. 1996;178:3818-3828
Mecillinam resistance in Escherichia coli is conferred by loss of a second activity of the AroK protein.
Vinella, D., B. Gagny, D. Joseleau-Petit, R. D'Ari, and M. Cashel.
|
J Bacteriol. 1995;177:1627-1629
A reassessment of the relationship between aroK- and aroL-encoded shikimate kinase enzymes of Escherichia coli.
Whipp, M. J., and A. J. Pittard.
|
| Sequences |
Amino acid
FASTA format
|
0001 MAEKRNIFLV GPMGAGKSTI GRQLAQQLNM EFYDSDQEIE KRTGADVGWV FDLEGEEGFR DREEKVINEL
0071 TEKQGIVLAT GGGSVKSRET RNRLSARGVV VYLETTIEKQ LARTQRDKKR PLLHVETPPR EVLEALANER
0141 NPLYEEIADV TIRTDDQSAK VVANQIIHML ESN
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gactctcgca atatcttatg aggtttcagt
-070 tcatgtcctg cggcgctctc tgagcgaagc gggtttatca ttaacgaata gtcttagtag taccgaaaaa
0001 atggcagaga aacgcaatat ctttctggtt gggcctatgg gtgccggaaa aagcactatt gggcgccagt
0071 tagctcaaca actcaatatg gaattttacg attccgatca agagattgag aaacgaaccg gagctgatgt
0141 gggctgggtt ttcgatttag aaggcgaaga aggcttccgc gatcgcgaag aaaaggtcat caatgagttg
0211 accgagaaac agggtattgt gctggctact ggcggcggct ctgtgaaatc ccgtgaaacg cgtaaccgtc
0281 tttccgctcg tggcgttgtc gtttatcttg aaacgaccat cgaaaagcaa cttgcacgca cgcagcgtga
0351 taaaaaacgc ccgttgctgc acgttgaaac accgccgcgt gaagttctgg aagcgttggc caatgaacgc
0421 aatccgctgt atgaagagat tgccgacgtg accattcgta ctgatgatca aagcgctaaa gtggttgcaa
0491 accagattat tcacatgctg gaaagcaact aattctggct ttatatacac tcgtctgcgg gtacagtaat
0561 taaggtggat gtcgcgttat ggagaggatt gtcgttactc tcggggaacg tagttaccca at
|
