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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Bacteriol. 1968;95(4):1283-94.
Mutants of Escherichia coli with an altered tryptophanyl-transfer ribonucleic acid synthetase.
Doolittle WF, Yanofsky C.
(
4869215
)
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Deletion
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OCL19,20-2
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
trpS
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Related strains
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Strains Search
|
Search strains by gene name:
trpS
Search strains by all related name:
trpS b3384 ECK3371 f334 JW3347
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General information (Go to Linear View: )
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Gene Name
|
trpS
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Alternative name
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b3384,ECK3371,f334,JW3347
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Location, Length
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3,510,656 - 3,511,660
(
-
)
;
75.67
min
;
1005
(bp) ,
334
(aa)
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Product
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tryptophanyl-tRNA synthetase
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Operon Name
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aroKB-damX-dam-rpe-gph-trpS
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Note
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tryptophan tRNA synthetase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation
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Function
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enzyme; Aminoacyl tRNA synthetases, tRNA modification
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Gene Ontology
|
GO:0000166
;
nucleotide binding ( trpS )
GO:0004812
;
aminoacyl-tRNA ligase activity ( trpS )
GO:0004830
;
tryptophan-tRNA ligase activity ( trpS )
GO:0005524
;
ATP binding ( trpS )
GO:0005737
;
cytoplasm ( trpS )
GO:0006412
;
translation ( trpS )
GO:0006418
;
tRNA aminoacylation for protein translation ( trpS )
GO:0006436
;
tryptophanyl-tRNA aminoacylation ( trpS )
GO:0016874
;
ligase activity ( trpS )
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PID
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1789786
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EC number
(KEGG Pathway)
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6.1.1.2
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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trpS
(
EG11030
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
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SYW_ECOLI
(
P00954
)
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- Protein name
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Tryptophanyl-tRNA synthetase
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- Synonyms
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EC 6.1.1.2, Tryptophan--tRNA ligase, TrpRS
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- Gene name
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Name=trpS; OrderedLocusNames=b3384;
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MMBR References
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J Biol Chem. 1982;257:6132-6136
The nucleotide sequence of the structural gene for Escherichia coli tryptophanyl-tRNA synthetase.
Hall, C. V., M. van Cleemput, K. H. Muench, and C. Yanofsky.
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J Bacteriol. 1981;148(3):941-9.
Cloning and characterization of the gene for Escherichia coli tryptophanyl-transfer ribonucleic acid synthetase.
Hall CV, Yanofsky C.
(
6171561
)
|
J Bacteriol. 1983;153(2):1120-3.
Indolmycin-mediated inhibition and stimulation of transcription at the trp promoter of Escherichia coli.
Bogosian G, Haydock PV, Somerville RL.
(
6337120
)
|
| Sequences |
Amino acid
FASTA format
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0001 MTKPIVFSGA QPSGELTIGN YMGALRQWVN MQDDYHCIYC IVDQHAITVR QDAQKLRKAT LDTLALYLAC
0071 GIDPEKSTIF VQSHVPEHAQ LGWALNCYTY FGELSRMTQF KDKSARYAEN INAGLFDYPV LMAADILLYQ
0141 TNLVPVGEDQ KQHLELSRDI AQRFNALYGE IFKVPEPFIP KSGARVMSLL EPTKKMSKSD DNRNNVIGLL
0211 EDPKSVVKKI KRAVTDSDEP PVVRYDVQNK AGVSNLLDIL SAVTGQSIPE LEKQFEGKMY GHLKGEVADA
0281 VSGMLTELQE RYHRFRNDEA FLQQVMKDGA EKASAHASRT LKAVYEAIGF VAKP
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gaggctatcg atctcagcca gcctgatgta
-070 atttatcagt ctataaatga ccttctgccc gcattagggc ttccgcatag cgaaaatcag gaatcgaaaa
0001 atgactaagc ccatcgtttt tagtggcgca cagccctcag gtgaattgac cattggtaac tacatgggtg
0071 cgctgcgtca gtgggtaaac atgcaggatg actaccattg catttactgt atcgttgacc aacacgcgat
0141 caccgtgcgc caggatgcac agaagctgcg taaagcgacg ctggatacgc tggccttgta tctggcttgt
0211 ggtatcgatc ctgagaaaag caccattttt gttcagtccc acgtgccgga acatgcacag ttaggctggg
0281 cactgaactg ctatacctac ttcggcgaac tgagtcgcat gacgcagttt aaagataaat ctgcgcgtta
0351 tgccgagaac atcaacgctg gtctgtttga ctatccggtg ctgatggcag cggacatcct gctgtatcaa
0421 actaatctgg taccggtggg tgaagaccag aaacagcacc tcgaactgag ccgcgatatt gcccagcgtt
0491 tcaacgcgct gtatggcgag atctttaagg tgccggagcc gtttattccg aaatctggcg cgcgcgtaat
0561 gtcgctgctg gagccgacca agaagatgtc caagtctgac gataatcgca ataacgttat cggcctgctg
0631 gaagatccga aatcggtagt gaagaaaatc aaacgtgcgg tcactgactc cgacgagccg ccggtagttc
0701 gctacgatgt gcagaacaaa gcgggcgttt ccaacctgtt ggatatcctt tcagcggtaa cgggccagag
0771 catcccagaa ctggaaaaac agttcgaagg caagatgtat ggtcatctga aaggtgaagt ggctgatgcc
0841 gtttccggta tgctgactga attgcaggaa cgctatcacc gtttccgcaa cgatgaagcc ttcctgcaac
0911 aggtgatgaa agatggcgcg gaaaaagcca gcgcgcacgc ttcccgtacg ctaaaagcgg tgtacgaagc
0981 gattggtttt gtggcgaagc cgtaattcac tattgctggc aaattgcgct ttgttcatgc cggatgcggc
1051 gtgaacgcct tatccggcct acaaagtaat gcacatattg caaggaaatg taggc
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