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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Genetika. 1985;21(6):1068-9.
[Generation of deletions in the region of the crp gene on the Escherichia coli chromosome]
Kulakauskas ST, Sukhodolets VV.
(
3896929
)
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Deletion
|
OCL20
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
cysG
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Related strains
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Strains Search
|
Search strains by gene name:
cysG
Search strains by all related name:
cysG b3368 ECK3356 JW3331 o457
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General information (Go to Linear View: )
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Gene Name
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cysG
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Alternative name
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b3368,ECK3356,JW3331,o457
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Location, Length
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3,495,850 - 3,497,223
(
+
)
;
75.35
min
;
1374
(bp) ,
457
(aa)
|
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Product
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fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogenase andsiroheme ferrochelatase/uroporphyrinogen methyltransferase
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Operon Name
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nirBDC-cysG
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Note
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uroporphyrinogen III methylase; sirohaeme biosynthesis
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Function
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enzyme; Biosynthesis of cofactors, carriers: Heme, porphyrin
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Gene Ontology
|
GO:0003824
;
catalytic activity ( cysG )
GO:0004851
;
uroporphyrin-III C-methyltransferase activity ( cysG )
GO:0005488
;
binding ( cysG )
GO:0005515
;
protein binding ( cysG )
GO:0006779
;
porphyrin biosynthetic process ( cysG )
GO:0008152
;
metabolic process ( cysG )
GO:0008168
;
methyltransferase activity ( cysG )
GO:0009236
;
cobalamin biosynthetic process ( cysG )
GO:0016491
;
oxidoreductase activity ( cysG )
GO:0016740
;
transferase activity ( cysG )
GO:0016829
;
lyase activity ( cysG )
GO:0043115
;
precorrin-2 dehydrogenase activity ( cysG )
GO:0051266
;
sirohydrochlorin ferrochelatase activity ( cysG )
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PID
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1789768
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EC number
(KEGG Pathway)
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1.3.1.76
2.1.1.107
4.99.1.4
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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cysG
(
EG10188
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
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CYSG_ECOLI
(
P11098
;
P76685
)
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- Protein name
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Siroheme synthase
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- Synonyms
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- Gene name
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Name=cysG; OrderedLocusNames=b3368, z4729, ECs4219;
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MMBR References
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Mol Microbiol. 1992;6(19):2805-13.
Transcriptional control, translation and function of the products of the five open reading frames of the Escherichia coli nir operon.
Harborne NR, Griffiths L, Busby SJ, Cole JA.
(
1435259
)
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Biochem J. 1981;193(3):861-7.
Prosthetic groups of the NADH-dependent nitrite reductase from Escherichia coli K12.
Jackson RH, Cornish-Bowden A, Cole JA.
(
7030314
)
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Mol Gen Genet. 1985;200(2):328-34.
Molecular cloning and functional analysis of the cysG and nirB genes of Escherichia coli K12, two closely-linked genes required for NADH-dependent nitrite reductase activity.
Macdonald H, Cole J.
(
2993824
)
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J Gen Microbiol. 1985;131(10):2771-82.
Isolation, characterization and complementation analysis of nirB mutants of Escherichia coli deficient only in NADH-dependent nitrite reductase activity.
MacDonald H, Pope NR, Cole JA.
(
3906030
)
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Eur J Biochem. 1990;191:315-323
Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome.
Peakman, T. C., J. Crouzet, J.-F. Mayaux, S. J. Busby, S. Mohan, N. Harborne, J. C. Wootton, R. Nicolson, and J. A. Cole.
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Eur J Biochem. 1990;191:325-331
Transcriptional control of the cysG gene of Escherichia coli K-12 during aerobic and anaerobic growth.
Peakman, T. C., S. J. Busby, and J. A. Cole.
|
FEBS Lett. 1993;335(1):57-60.
The Escherichia coli cysG gene encodes the multifunctional protein, siroheme synthase.
Spencer JB, Stolowich NJ, Roessner CA, Scott AI.
(
8243665
)
|
Biotechnol Appl Biochem. 1990;12(2):212-6.
Molecular cloning of the cys genes (cysC, cysD, cysH, cysI, cysJ, and cysG) responsible for cysteine biosynthesis in Escherichia coli K-12.
Tei H, Murata K, Kimura A.
(
2158792
)
|
J Gen Microbiol. 1980;120(2):475-83.
Biochemical and genetic characterization of nirB mutants of Escherichia coli K 12 pleiotropically defective in nitrite and sulphite reduction.
Cole JA, Newman BM, White P.
(
6262434
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MDHLPIFCQL RDRDCLIVGG GDVAERKARL LLDAGARLTV NALAFIPQFT AWADAGMLTL VEGPFDESLL
0071 DTCWLAIAAT DDDALNQRVS EAAEARRIFC NVVDAPKAAS FIMPSIIDRS PLMVAVSSGG TSPVLARLLR
0141 EKLESLLPLH LGQVAKYAGQ LRGRVKQQFA TMGERRRFWE KLFVNDRLAQ SLANNDQKAI TETTEQLINE
0211 PLDHRGEVVL VGAGPGDAGL LTLKGLQQIQ QADVVVYDRL VSDDIMNLVR RDADRVFVGK RAGYHCVPQE
0281 EINQILLREA QKGKRVVRLK GGDPFIFGRG GEELETLCNA GIPFSVVPGI TAASGCSAYS GIPLTHRDYA
0351 QSVRLITGHL KTGGELDWEN LAAEKQTLVF YMGLNQAATI QQKLIEHGMP GEMPVAIVEN GTAVTQRVID
0421 GTLTQLGELA QQMNSPSLII IGRVVGLRDK LNWFSNH
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ATTGGGTTAT TGGTATGCTA CGCCGAAAGC
-070 GAATCGTCCG GTTGCGGACA AATTTAATCA AACTGAAACG GCTGCCGGTT AATTACTAAG GGGTTTTTAC
0001 gtggatcatt tgcctatatt ttgccaatta cgcgatcgcg actgtctgat tgtcggcggt ggtgatgtcg
0071 cggaacgcaa agcaaggttg ctgttagacg caggcgctcg cttaacggtg aatgcattag cgtttattcc
0141 acagttcacc gcatgggcag atgcaggcat gttaaccctc gtcgaagggc catttgatga aagccttctc
0211 gacacctgct ggctggcgat tgcagcgacg gatgatgacg cgcttaacca gcgcgtcagc gaagccgctg
0281 aagctcgtcg catcttctgt aacgtggtcg atgcgccgaa agccgccagc tttattatgc cgtcgattat
0351 tgaccgctca ccgctcatgg tagcggtctc ctctggcggc acctctccgg ttctggcacg cctgttgcgc
0421 gaaaaacttg aatcactgct gccgttacat ctgggccagg tagcgaaata cgccgggcaa ttacgcgggc
0491 gagtgaaaca acagttcgcc acgatgggtg agcgtcgccg tttctgggag aaattgttcg ttaacgaccg
0561 cctggcgcag tcgctggcaa acaacgatca gaaagccatt actgaaacga ccgaacagtt aatcaacgaa
0631 ccgctcgacc atcgcggtga agtggtgctg gttggtgcag gtccgggcga tgccgggctg ctgacactga
0701 aaggactgca acaaattcag caggcagatg tggtggtcta cgaccgtctg gtttctgacg atattatgaa
0771 tctggtacgc cgcgatgcgg accgtgtttt cgtcggcaaa cgcgcgggat accactgcgt accccaggaa
0841 gagattaacc agatcctgct gcgggaagcg caaaaaggca aacgcgtggt gcggctgaaa ggtggcgatc
0911 cgtttatttt tggccgtggt ggcgaagagc tggaaacact gtgcaacgcg ggtattccgt tctcggtggt
0981 tccgggtatt accgcagctt ctggttgctc tgcctattcg ggtattccac tcacgcatcg cgattatgcc
1051 cagagcgtac gcttaattac cggacactta aaaaccggtg gcgagctgga ctgggaaaac ctggcggcag
1121 aaaaacagac gctggtgttc tatatggggt tgaatcaggc cgcgactatt cagcaaaagc tgattgaaca
1191 cggaatgcca ggcgaaatgc cggtggcaat tgtcgaaaac ggtacggcag tcacgcagcg cgtgattgac
1261 ggtacgctca cacagctggg agaactggcg cagcaaatga acagtccatc gctaattatt attggtcggg
1331 ttgttggcct gcgcgataaa ctgaactggt tctccaacca ttaaTTTAAC CCGGGCCAGA GAATTCTGGC
1401 CTTCTTAATG ACTCTTTTTA TATATTCAGC AAATAAAACA TAGCCCCTTA ATAAATATTA TCTGCTAATG
1471 GGTT
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