MG1655
W3110
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Gene Report : nirD
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCL20 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  nirD
Related strains
     Strains Search Search strains by gene name:  nirD   Search strains by all related name:  nirD b3366 ECK3354 JW3329 o108

General information  (Go to Linear View:)
 Gene Name nirD  
 Alternative name b3366,ECK3354,JW3329,o108  
 Location, Length 3,494,573 - 3,494,899 (  +  ) ;   75.32 min ; 327 (bp) ,   108 (aa) Go to Linear View
 Product nitrite reductase, NAD(P)H-binding, small subunit  
 Operon Name nirBDC-cysG  
 Note nitrite reductase (NAD(P)H) subunit; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009061 - anaerobic respiration  
 Function enzyme; Energy metabolism, carbon: Anaerobic respiration  
 Gene Ontology GO:0005737 ; cytoplasm ( nirD )
GO:0006118 ; electron transport ( nirD )
GO:0008942 ; nitrite reductase [NAD(P)H] activity ( nirD )
GO:0016491 ; oxidoreductase activity ( nirD )
GO:0042128 ; nitrate assimilation ( nirD )
 PID 1789766  
 EC number
  (KEGG Pathway)
 
1.7.1.4  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) NIRD_ECOLI ( P23675 )
    -  Protein name Nitrite reductase [NAD(P)H] small subunit  
    -  Synonyms EC 1.7.1.4  
    -  Gene name Name=nirD; OrderedLocusNames=b3366, z4727, ECs4217;  

 Linear View (Whole Mode)
View Location
   3485.0  –  3510.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP nirD (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
        PSI-BLAST  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Nitrite reductase [NAD(P)H] small subunit  
         PSI-BLAST Nitrite reductase [NAD(P)H] small subunit
    nr     (database updated : 2007.02.20 )
         BLAST nitrite reductase small subunit [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    GRAM_POS_ANCHORING     

Other Cross-Reference
    COG COG2146PR 
    EcoCyc nirD 

MMBR References
Mol Microbiol. 1992;6(19):2805-13.
Transcriptional control, translation and function of the products of the five open reading frames of the Escherichia coli nir operon.
Harborne NR, Griffiths L, Busby SJ, Cole JA. ( 1435259 )
Eur J Biochem. 1990;191:315-323
Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome.
Peakman, T. C., J. Crouzet, J.-F. Mayaux, S. J. Busby, S. Mohan, N. Harborne, J. C. Wootton, R. Nicolson, and J. A. Cole.

Sequences
Amino acid
FASTA format
0001 MSQWKDICKI DDILPETGVC ALLGDEQVAI FRPYHSDQVF AISNIDPFFE SSVLSRGLIA EHQGELWVAS 
0071 PLKKQRFRLS DGLCMEDEQF SVKHYEARVK DGVVQLRG

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             ACAAGCGTGA CCCGAACGTG CAGATGGTGC 
-070 CAGAGCGCGA ACAGCACCGT CCGGCAACGC CGTATGAACG TATCCCAGTA ACTCTGGTGG AGGACAACGC 
0001 atgagccagt ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag 
0071 gtgacgagca ggtcgcgatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca acatcgaccc 
0141 gttcttcgag tccagcgtgc tgtcacgcgg actgattgcg gaacaccagg gcgagctgtg ggtcgccagc 
0211 ccgctgaaaa aacagcgttt tcgcttaagc gacggcttgt gcatggaaga cgaacagttt tccgtcaaac 
0281 attacgaagc gcgagtgaaa gacggcgtgg tgcagctgcg cggttaaTGT TTTAACGGGA GGCGCAATGC 
0351 CTCCCCTTTT TGCATGGTCC TGTAATAATC TTCGGTATAT TGCAGGACAT TTTTTAAACT TTTTGTTTTA 
0421 TTTTTTG