|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
J Gen Microbiol. 1980;120(2):475-83.
Biochemical and genetic characterization of nirB mutants of Escherichia coli K 12 pleiotropically defective in nitrite and sulphite reduction.
Cole JA, Newman BM, White P.
(
6262434
)
|
|
|
Deletion
|
OCL20
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
nirB
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
nirB
Search strains by all related name:
nirB b3365 ECK3353 JW3328 o847
|
General information (Go to Linear View: )
|
|
Gene Name
|
nirB
|
|
Alternative name
|
b3365,ECK3353,JW3328,o847
|
|
Location, Length
|
3,492,033 - 3,494,576
(
+
)
;
75.26
min
;
2544
(bp) ,
847
(aa)
|
|
Product
|
nitrite reductase, large subunit, NAD(P)H-binding
|
|
Operon Name
|
nirBDC-cysG
|
|
Note
|
nitrite reductase (NAD(P)H) subunit; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009061 - anaerobic respiration
|
|
Function
|
enzyme; Energy metabolism, carbon: Anaerobic respiration
|
|
Gene Ontology
|
GO:0005506
;
iron ion binding ( nirB )
GO:0006118
;
electron transport ( nirB )
GO:0008942
;
nitrite reductase [NAD(P)H] activity ( nirB )
GO:0016491
;
oxidoreductase activity ( nirB )
GO:0020037
;
heme binding ( nirB )
GO:0042128
;
nitrate assimilation ( nirB )
GO:0046872
;
metal ion binding ( nirB )
GO:0050660
;
FAD binding ( nirB )
GO:0050661
;
NADP binding ( nirB )
GO:0051536
;
iron-sulfur cluster binding ( nirB )
GO:0051539
;
4 iron, 4 sulfur cluster binding ( nirB )
|
|
PID
|
1789765
|
EC number
(KEGG Pathway)
|
1.7.1.4
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
NIRB_ECOLI
(
P08201
)
|
|
- Protein name
|
Nitrite reductase [NAD(P)H] large subunit
|
|
- Synonyms
|
EC 1.7.1.4
|
|
- Gene name
|
Name=nirB; OrderedLocusNames=b3365;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
nirB
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of Mouse Thioredoxin Reductase Type 2
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Nitrite reductase [NAD
|
|
PSI-BLAST
|
Nitrite reductase [NAD
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
nitrite reductase, large subunit, NAD(P)H-binding [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
GLYCOSAMINOGLYCAN
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
NIR_SIR
|
|
MMBR References
|
Mol Microbiol. 1992;6(19):2805-13.
Transcriptional control, translation and function of the products of the five open reading frames of the Escherichia coli nir operon.
Harborne NR, Griffiths L, Busby SJ, Cole JA.
(
1435259
)
|
Biochem J. 1981;193(3):861-7.
Prosthetic groups of the NADH-dependent nitrite reductase from Escherichia coli K12.
Jackson RH, Cornish-Bowden A, Cole JA.
(
7030314
)
|
J Mol Biol. 1987;196(4):781-8.
Location and sequence of the promoter of the gene for the NADH-dependent nitrite reductase of Escherichia coli and its regulation by oxygen, the Fnr protein and nitrite.
Jayaraman PS, Peakman TC, Busby SJ, Quincey RV, Cole JA.
(
2445993
)
|
Mol Gen Genet. 1985;200(2):328-34.
Molecular cloning and functional analysis of the cysG and nirB genes of Escherichia coli K12, two closely-linked genes required for NADH-dependent nitrite reductase activity.
Macdonald H, Cole J.
(
2993824
)
|
J Gen Microbiol. 1985;131(10):2771-82.
Isolation, characterization and complementation analysis of nirB mutants of Escherichia coli deficient only in NADH-dependent nitrite reductase activity.
MacDonald H, Pope NR, Cole JA.
(
3906030
)
|
Eur J Biochem. 1990;191:315-323
Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome.
Peakman, T. C., J. Crouzet, J.-F. Mayaux, S. J. Busby, S. Mohan, N. Harborne, J. C. Wootton, R. Nicolson, and J. A. Cole.
|
| Sequences |
Amino acid
FASTA format
|
0001 MSKVRLAIIG NGMVGHRFIE DLLDKSDAAN FDITVFCEEP RIAYDRVHLS SYFSHHTAEE LSLVREGFYE
0071 KHGIKVLVGE RAITINRQEK VIHSSAGRTV FYDKLIMATG SYPWIPPIKG SDTQDCFVYR TIEDLNAIES
0141 CARRSKRGAV VGGGLLGLEA AGALKNLGIE THVIEFAPML MAEQLDQMGG EQLRRKIESM GVRVHTSKNT
0211 LEIVQEGVEA RKTMRFADGS ELEVDFIVFS TGIRPRDKLA TQCGLDVAPR GGIVINDSCQ TSDPDIYAIG
0281 ECASWNNRVF GLVAPGYKMA QVAVDHILGS ENAFEGADLS AKLKLLGVDV GGIGDAHGRT PGARSYVYLD
0351 ESKEIYKRLI VSEDNKTLLG AVLVGDTSDY GNLLQLVLNA IELPENPDSL ILPAHSGSGK PSIGVDKLPD
0421 SAQICSCFDV TKGDLIAAIN KGCHTVAALK AETKAGTGCG GCIPLVTQVL NAELAKQGIE VNNNLCEHFA
0491 YSRQELFHLI RVEGIKTFEE LLAKHGKGYG CEVCKPTVGS LLASCWNEYI LKPEHTPLQD SNDNFLANIQ
0561 KDGTYSVIPR SPGGEITPEG LMAVGRIARE FNLYTKITGS QRLAMFGAQK DDLPEIWRQL IEAGFETGHA
0631 YAKALRMAKT CVGSTWCRYG VGDSVGLGVE LENRYKGIRT PHKMKFGVSG CTRECSEAQG KDVGIIATEK
0701 GWNLYVCGNG GMKPRHADLL AADIDRETLI KYLDRFMMFY IRTADKLTRT APWLENLEGG IDYLKAVIID
0771 DKLGLNAHLE EEMARLREAV LCEWTETVNT PSAQTRFKHF INSDKRDPNV QMVPEREQHR PATPYERIPV
0841 TLVEDNA
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ACCCATTAAG GAGTATATAA AGGTGAATTT
-070 GATTTACATC AATAAGCGGG GTTGCTGAAT CGTTAAGGTA GGCGGTAATA GAAAAGAAAT CGAGGCAAAA
0001 atgagcaaag tcagactcgc aattatcggt aacggtatgg tcggccatcg ctttatcgaa gatcttcttg
0071 ataaatctga tgcggccaac tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt
0141 acacctctcg tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
0211 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag gtgattcact
0281 ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt tcctacccgt ggatcccgcc
0351 aatcaaaggt tctgatactc aggactgctt tgtctatcgc actattgaag acctcaacgc cattgaatcc
0421 tgcgcccgtc gcagcaaacg cggtgccgtt gttggtggcg gcctgttagg tctggaagcc gcaggcgcgc
0491 tgaaaaactt aggtattgaa acccacgtta tcgaatttgc ccctatgctg atggcagaac agcttgatca
0561 gatgggcggc gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
0631 cttgagattg tgcaggaagg tgttgaagcg cgtaaaacca tgcgttttgc cgacggcagc gaactggaag
0701 tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca acccagtgtg gtctggacgt
0771 tgctccgcgt gggggtattg tcattaatga ttcctgccag acttccgatc cggatatcta cgccatcggt
0841 gaatgcgcaa gctggaacaa ccgtgtattt ggtctggtag cacctggcta caaaatggcg caggtcgccg
0911 ttgaccatat tctcggtagc gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg
0981 cgtagacgta ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
1051 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt gcggtactgg
1121 tgggcgatac cagcgactac ggtaacctgc tgcaactggt gctgaacgct atcgaactgc cggaaaaccc
1191 ggattccctg atcctgccag cacactcggg tagcggcaag ccgtctatcg gtgttgataa actgccggac
1261 agcgcgcaaa tctgctcctg cttcgacgtc accaaaggtg atctgattgc tgccatcaac aaaggctgcc
1331 acacagttgc ggcgctgaaa gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac
1401 tcaggtactg aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
1471 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa ctgctggcga
1541 aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg ctgctggcct cctgctggaa
1611 cgaatacatt ctgaagccgg aacatactcc gctgcaggat tctaacgaca acttcctcgc taacatccag
1681 aaagacggca cctactcggt gatcccgcgt tctccgggcg gtgaaatcac cccggaaggg ctgatggcgg
1751 taggtcgtat cgcgcgtgaa tttaatctct acaccaagat cactggctcc cagcgtctgg cgatgtttgg
1821 cgcacagaaa gacgatctgc cggagatctg gcgtcagctg attgaagccg gcttcgaaac cggtcatgcc
1891 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc gttggcgaca
1961 gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg ccgcacaaaa tgaagttcgg
2031 tgtctccggc tgtacccgtg aatgttcaga agctcagggt aaagacgtgg gtattatcgc cactgaaaaa
2101 ggctggaacc tgtatgtttg cggtaacggc ggcatgaaac cgcgtcatgc ggatctgctg gcggcggata
2171 tcgatcgcga aacgctgatc aaatatctcg accgcttcat gatgttctac atccgtactg ccgacaaact
2241 gacgcgtacc gcaccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcagt gatcattgac
2311 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta ctgtgtgagt
2381 ggactgaaac ggtcaatacg ccgtctgcgc agactcgctt caaacacttc atcaacagcg acaagcgtga
2451 cccgaacgtg cagatggtgc cagagcgcga acagcaccgt ccggcaacgc cgtatgaacg tatcccagta
2521 actctggtgg aggacaacgc atgaGCCAGT GGAAAGACAT CTGCAAAATC GATGACATCC TGCCTGAAAC
2591 CGGCGTCTGC GCGCTGTTAG GTGACGAGCA GGTCGCGATT TTCCGCCCGT ATCA
|
