MG1655
W3110
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Gene Report : carA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR28 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  carA
Related strains
     Strains Search Search strains by gene name:  carA   Search strains by all related name:  carA arg arg+ura b0032 cap ECK0033 JW0030 pyrA

General information  (Go to Linear View:)
 Gene Name carA  
 Alternative name arg,arg+ura,b0032,cap,ECK0033,JW0030,pyrA  
 Location, Length 29,651 - 30,799 (  +  ) ;   0.64 min ; 1149 (bp) ,   382 (aa) Go to Linear View
 Product carbamoyl phosphate synthetase small subunit, glutamine amidotransferase  
 Operon Name carAB  
 Note GO_process: GO:0006526 - arginine biosynthetic process; GO_process: GO:0006221 - pyrimidine nucleotide biosynthetic process  
 Function enzyme; Pyrimidine ribonucleotide biosynthesis,1.5.1.3 metabolism; building block biosynthesis; amino acids; arginine,1.5.2.2 metabolism; building block biosynthesis; nucleotide; pyrimidine biosynthesis  
 Gene Ontology GO:0003824; catalytic activity ( carA )
GO:0004086; carbamoyl-phosphate synthase activity ( carA )
GO:0004088; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity ( carA )
GO:0005515; protein binding ( carA )
GO:0005524; ATP binding ( carA )
GO:0006221; pyrimidine nucleotide biosynthetic process ( carA )
GO:0006526; arginine biosynthetic process ( carA )
GO:0006541; glutamine metabolic process ( carA )
GO:0006807; nitrogen compound metabolic process ( carA )
GO:0008652; amino acid biosynthetic process ( carA )
GO:0009058; biosynthetic process ( carA )
GO:0016874; ligase activity ( carA )
 PID 1786215  
 EC number
  (KEGG Pathway)
 
6.3.5.5  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene carA ( EG10134 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) CARA_ECOLI ( P00907 )
    -  Protein name Carbamoyl-phosphate synthase small chain  
    -  Synonyms EC 6.3.5.5, Carbamoyl- phosphate synthetase glutamine chain  
    -  Gene name Name=carA; Synonyms=pyrA; OrderedLocusNames=b0032, z0037, ECs0035;  

 Linear View (Whole Mode)
View Location
   20.0  –  45.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP carA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain C, Carbamoyl Phosphate Synthetase From Escherichia Coli  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Carbamoyl-phosphate synthase small chain (Carbamoyl-phosphate synthetase glutamine chain)  
         PSI-BLAST Carbamoyl-phosphate synthase small chain
    nr     (database updated : 2007.02.20 )
         BLAST carbamoyl-phosphate synthase small subunit [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    PROKAR_LIPOPROTEIN    GATASE_TYPE_I     

Other Cross-Reference
    COG COG0505EF 
    EcoCyc carA 

MMBR References
J Bacteriol. 1980;143(2):914-20.
Patterns of polarity in the Escherichia coli car AB gene cluster.
Gigot D, Crabeel M, Feller A, Charlier D, Lissens W, Glansdorff N, Pierard A. ( 6162837 )
Proc Natl Acad Sci U S A. 1984;81:4134-4138
DNA sequence of the carA gene and the control region of carAB: tandem promoters, respectively controlled by arginine and the pyrimidines, regulate the synthesis of carbamoylphosphate.
Piette, J., H. Nyunoya, C. J. Lusty, R. Cunin, G. Weyens, M. Crabeel, D. Charlier, N. Glansdorff, and A. Pierard.
Proc Natl Acad Sci U S A. 1984;81:4139-4143
Multiple regulatory signals in the control region of the Escherichia coli carAB operon.
Bouvier, J., J. C. Patte, and P. Stragier.
J Bacteriol. 1980;143(2):921-5.
Use of gene cloning to determine polarity of an operon: genes carAB of Escherichia coli.
Crabeel M, Charlier D, Weyens G, Feller A, Pierard A, Glansdorff N. ( 6451616 )

Sequences
Amino acid
FASTA format
0001 MIKSALLVLE DGTQFHGRAI GATGSAVGEV VFNTSMTGYQ EILTDPSYSR QIVTLTYPHI GNVGTNDADE 
0071 ESSQVHAQGL VIRDLPLIAS NFRNTEDLSS YLKRHNIVAI ADIDTRKLTR LLREKGAQNG CIIAGDNPDA 
0141 ALALEKARAF PGLNGMDLAK EVTTAEAYSW TQGSWTLTGG LPEAKKEDEL PFHVVAYDFG AKRNILRMLV 
0211 DRGCRLTIVP AQTSAEDVLK MNPDGIFLSN GPGDPAPCDY AITAIQKFLE TDIPVFGICL GHQLLALASG 
0281 AKTVKMKFGH HGGNHPVKDV EKNVVMITAQ NHGFAVDEAT LPANLRVTHK SLFDGTLQGI HRTDKPAFSF 
0351 QGHPEASPGP HDAAPLFDHF IELIEQYRKT AK

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             GTTTGCCAGA AATTCGTCGG TAAGCAGATT 
-070 TGCATTGATT TACGTCATCA TTGTGAATTA ATATGCAAAT AAAGTGAGTG AATATTCTCT GGAGGGTGTT 
0001 ttgattaagt cagcgctatt ggttctggaa gacggaaccc agtttcacgg tcgggccata ggggcaacag 
0071 gttcggcggt tggggaagtc gttttcaata cttcaatgac cggttatcaa gaaatcctca ctgatccttc 
0141 ctattctcgt caaatcgtta ctcttactta tccccatatt ggcaatgtcg gcaccaatga cgccgatgaa 
0211 gaatcttctc aggtacatgc acaaggtctg gtgattcgcg acctgccgct gattgccagc aacttccgta 
0281 ataccgaaga cctctcttct tacctgaaac gccataacat cgtggcgatt gccgatatcg atacccgtaa 
0351 gctgacgcgt ttactgcgcg agaaaggcgc acagaatggc tgcattatcg cgggcgataa cccggatgcg 
0421 gcgctggcgt tagaaaaagc ccgcgcgttc ccaggtctga atggcatgga tctggcaaaa gaagtgacca 
0491 ccgcagaagc ctatagctgg acacaaggga gctggacgtt gaccggtggc ctgccagaag cgaaaaaaga 
0561 agacgagctg ccgttccacg tcgtggctta tgattttggt gccaagcgca acatcctgcg gatgctggtg 
0631 gatagaggct gtcgcctgac catcgttccg gcgcaaactt ctgcggaaga tgtgctgaaa atgaatccag 
0701 acggcatctt cctctccaac ggtcctggcg acccggcccc gtgcgattac gccattaccg ccatccagaa 
0771 attcctcgaa accgatattc cggtattcgg catctgtctc ggtcatcagc tgctggcgct ggcgagcggt 
0841 gcgaagactg tcaaaatgaa atttggtcac cacggcggca accatccggt taaagatgtg gagaaaaacg 
0911 tggtaatgat caccgcccag aaccacggtt ttgcggtgga cgaagcaaca ttacctgcaa acctgcgtgt 
0981 cacgcataaa tccctgttcg acggtacgtt acagggcatt catcgcaccg ataaaccggc attcagcttc 
1051 caggggcacc ctgaagccag ccctggtcca cacgacgccg cgccgttgtt cgaccacttt atcgagttaa 
1121 ttgagcagta ccgtaaaacc gctaagtaaT CAGGAGTAAA AGAGCCATGC CAAAACGTAC AGATATAAAA 
1191 AGTATCCTGA TTCTGGGTGC GGGCCCGATT GTTATCGGTC AGGCGTGTGA GTTTGACTA