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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Genetika. 1985;21(6):1068-9.
[Generation of deletions in the region of the crp gene on the Escherichia coli chromosome]
Kulakauskas ST, Sukhodolets VV.
(
3896929
)
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Deletion
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OCL21
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
crp
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Related strains
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Strains Search
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Search strains by gene name:
crp
Search strains by all related name:
crp b3357 cap csm ECK3345 JW5702 o210
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General information (Go to Linear View: )
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Gene Name
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crp
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Alternative name
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b3357,cap,csm,ECK3345,JW5702,o210
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Location, Length
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3,484,142 - 3,484,774
(
+
)
;
75.09
min
;
633
(bp) ,
210
(aa)
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Product
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DNA-binding transcriptional dual regulator
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Operon Name
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crp
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Note
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cyclic AMP receptor protein; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0016563 - transcription activator activity; GO_function: GO:0016564 - transcription repressor activity; GO_process: GO:0006350 - transcription
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Function
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regulator; Global regulatory functions
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Gene Ontology
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GO:0000166
;
nucleotide binding ( crp )
GO:0003677
;
DNA binding ( crp )
GO:0003700
;
transcription factor activity ( crp )
GO:0005515
;
protein binding ( crp )
GO:0005622
;
intracellular ( crp )
GO:0006350
;
transcription ( crp )
GO:0006355
;
regulation of transcription, DNA-dependent ( crp )
GO:0030552
;
cAMP binding ( crp )
GO:0045449
;
regulation of transcription ( crp )
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PID
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1789756
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EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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crp
(
EG10164
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
)
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Entry name(Acc.no)
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CRP_ECOLI
(
P03020
)
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- Protein name
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Catabolite gene activator
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- Synonyms
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cAMP receptor protein, cAMP-regulatory protein
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- Gene name
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OrderedLocusNames=b3357, c4132, z4718, ECs4208, SF3376, S4387;
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MMBR References
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J Bacteriol. 1986;166(2):533-40.
Cloning and molecular characterization of csm mutations allowing expression of catabolite-repressible operons in the absence of exogenous cyclic AMP.
George SE, Melton T.
(
3009405
)
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J Bacteriol. 1974;120(1):542-4.
Use of streptomycin and cyclic adenosine 5'-monophosphate in the isolation of mutants deficient in CAP protein.
Artman M, Werthamer S.
(
4371357
)
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J Bacteriol. 1981;148(3):753-61.
Isolation and characterization of an Escherichia coli mutant affected in the regulation of adenylate cyclase.
Guidi-Rontani C, Danchin A, Ullmann A.
(
6273380
)
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Proc Natl Acad Sci U S A. 1997;94:12914-12919
cAMP receptor protein-cAMP plays a crucial role in glucose-lactose diauxie by activating the major glucose transporter gene in Escherichia coli.
Kimata, K., H. Takahashi, T. Inada, P. Postma, and H. Aiba.
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J Bacteriol. 1976;125(2):545-55.
Properties of adenyl cyclase and cyclic adenosine 3',5'-monophosphate receptor protein-deficient mutants of Escherichia coli.
Kumar S.
(
173710
)
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J Bacteriol. 1973;116(2):957-64.
Mutants of Escherichia coli K-12 "cryptic," or deficient in 5'-nucleotidase (uridine diphosphate-sugar hydrolase) and 3'-nucleotidase (cyclic phosphodiesterase) activity.
Beacham IR, Kahana R, Levy L, Yagil E.
(
4355492
)
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Biol Chem. 1997;378(10):1153-62.
A lethal mutant of the catabolite gene activator protein CAP of Escherichia coli.
Lopata M, Schlieper D, von Wilcken-Bergmann B, Muller-Hill B.
(
9372184
)
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Nucleic Acids Res. 1982;10:1345-1361
Molecular cloning and nucleotide sequencing of the gene for E. coli cAMP receptor protein.
Aiba, H., S. Fujimoto, and N. Ozaki.
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Proc Natl Acad Sci U S A. 1986;83:5000-5004
Mechanism for the autogenous control of the crp operon: transcriptional inhibition by a divergent RNA transcript.
Okamoto, K., and M. Freundlich.
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Mutat Res. 1992;282(4):247-52.
UV resistance of E. coli K-12 deficient in cAMP/CRP regulation.
Puyo MF, Calsou P, Salles B.
(
1379686
)
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Microbiology 1995;141:1901-1907
cAMP-cAMP receptor protein complex: five binding sites in the control region of the Escherichia coli mannitol operon.
Ramseier, T. M., and M. H. Saier, Jr.
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Proc Natl Acad Sci U S A. 1996;93:10151-10155
Dual-function regulators: the cAMP receptor protein and the CytR regulator can act either to repress or to activate transcription depending on the context.
Rasmussen, P. B., B. Holst, and P. Valentin-Hansen.
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Sov. Genet. (Engl. Transl. Genetika) 1986;22:432-438.
Construction of the hybrid crp-lac operon and investigation of the role of the CRP-cAMP complex in its regulation in Escherichia coli.
Smirnov, Y. V., and A. F. Lisenkov.
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Genetics 1972;71:207-216
On some genetic aspects of phage lambda resistance in E. coli K12.
Thirion, J. P., and M. Hofnung.
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J Bacteriol. 1973;116(2):582-7.
Genetic characterization of mutations which affect catabolite-sensitive operons in Escherichia coli, including deletions of the gene for adenyl cyclase.
Brickman E, Soll L, Beckwith JR.
(
4583241
)
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J Bacteriol. 1974;119(2):357-62.
Accumulation of toxic concentrations of methylglyoxal by wild-type Escherichia coli K-12.
Ackerman RS, Cozzarelli NR, Epstein W.
(
4604054
)
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Nucleic Acids Res. 1982;10:1363-1378
Cloning and sequencing of the crp gene of Escherichia coli K12.
Cossart, P., and B. Gicquel-Sanzey.
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Mol Gen Genet. 1979;176(3):343-50.
Involvement of cyclic AMP and its receptor protein in the sensitivity of Escherichia coli K 12 toward serine: excretion of 2-ketobutyrate, a precursor of isoleucine.
Daniel J, Danchin A.
(
230407
)
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| Sequences |
Amino acid
FASTA format
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0001 MVLGKPQTDP TLEWFLSHCH IHKYPSKSTL IHQGEKAETL YYIVKGSVAV LIKDEEGKEM ILSYLNQGDF
0071 IGELGLFEEG QERSAWVRAK TACEVAEISY KKFRQLIQVN PDILMRLSAQ MARRLQVTSE KVGNLAFLDV
0141 TGRIAQTLLN LAKQPDAMTH PDGMQIKITR QEIGQIVGCS RETVGRILKM LEDQNLISAH GKTIVVYGTR
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 TGATAGCCCC TTCCCAGGTA GCGGGAAGCA
-070 TATTTCGGCA ATCCAGAGAC AGCGGCGTTA TCTGGCTCTG GAGAAAGCTT ATAACAGAGG ATAACCGCGC
0001 atggtgcttg gcaaaccgca aacagacccg actctcgaat ggttcttgtc tcattgccac attcataagt
0071 acccatccaa gagcacgctt attcaccagg gtgaaaaagc ggaaacgctg tactacatcg ttaaaggctc
0141 tgtggcagtg ctgatcaaag acgaagaggg taaagaaatg atcctctcct atctgaatca gggtgatttt
0211 attggcgaac tgggcctgtt tgaagagggc caggaacgta gcgcatgggt acgtgcgaaa accgcctgtg
0281 aagtggctga aatttcgtac aaaaaatttc gccaattgat tcaggtaaac ccggacattc tgatgcgttt
0351 gtctgcacag atggcgcgtc gtctgcaagt cacttcagag aaagtgggca acctggcgtt cctcgacgtg
0421 acgggccgca ttgcacagac tctgctgaat ctggcaaaac aaccagacgc tatgactcac ccggacggta
0491 tgcaaatcaa aattacccgt caggaaattg gtcagattgt cggctgttct cgtgaaaccg tgggacgcat
0561 tctgaagatg ctggaagatc agaacctgat ctccgcacac ggtaaaacca tcgtcgttta cggcactcgt
0631 taaTCCCGTC GGAGTGGCGC GTTACCTGGT AGCGCGCCAT TTTGTTTCCC CCGATGTGGC GCAGACTGAT
0701 TTATCACCCC GATATCAACT ATGCACTTCG ACA
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