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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Bacteriol. 1994;176(1):123-9.
In vivo selection of conditional-lethal mutations in the gene encoding elongation factor G of Escherichia coli.
Hou Y, Lin YP, Sharer JD, March PE.
(
8282687
)
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Deletion
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OCL22-6
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
fusA
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Related strains
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Strains Search
|
Search strains by gene name:
fusA
Search strains by all related name:
fusA b3340 ECK3327 f704 far fus JW3302
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General information (Go to Linear View: )
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Gene Name
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fusA
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Alternative name
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b3340,ECK3327,f704,far,fus,JW3302
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Location, Length
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3,469,422 - 3,471,536
(
-
)
;
74.78
min
;
2115
(bp) ,
704
(aa)
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Product
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protein chain elongation factor EF-G, GTP-binding
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Operon Name
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rpsLG-fusA-tufA
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Note
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GTP-binding protein chain elongation factor EF-G; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006412 - translation
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Function
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factor; Proteins - translation and modification
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Gene Ontology
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GO:0000166
;
nucleotide binding ( fusA )
GO:0003746
;
translation elongation factor activity ( fusA )
GO:0003924
;
GTPase activity ( fusA )
GO:0005515
;
protein binding ( fusA )
GO:0005525
;
GTP binding ( fusA )
GO:0005737
;
cytoplasm ( fusA )
GO:0006412
;
translation ( fusA )
GO:0006414
;
translational elongation ( fusA )
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PID
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1789738
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EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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fusA
(
EG10360
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
)
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Entry name(Acc.no)
|
EFG_ECOLI
(
P02996
;
Q9F439
)
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- Protein name
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Elongation factor G
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- Synonyms
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EF-G
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- Gene name
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OrderedLocusNames=b3340, c4112, z4698, ECs4191;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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fusA
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Ribosomal Elongation Factor G
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Elongation factor G (EF-G)
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PSI-BLAST
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Elongation factor G
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nr
(database updated :
2007.02.20
)
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BLAST
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elongation factor EF-2 [Escherichia coli O157:H7 EDL933]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
ATP_GTP_A
EFACTOR_GTP
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MMBR References
|
J Bacteriol. 1994;176:123-129
In vivo selection of conditional-lethal mutations in the gene encoding elongation factor G of Escherichia coli.
Hou, Y., Y.-P. Lin, J. D. Sharer, and P. E. March.
|
Biochem Biophys Res Commun. 1971;42(3):441-4.
G factor mutants of Escherichia coli: map location and properties.
Kuwano M, Schlessinger D.
(
5542892
)
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J Biol Chem. 1980;255:4660-4666
DNA sequences from the str operon of Escherichia coli.
Post, L., and M. Nomura.
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Nucleic Acids Res. 1984;12:2181-2192
The nucleotide sequence of the Escherichia coli fus gene, coding for elongation factor G.
Zengel, J. M., R. H. Archer, and L. Lindahl.
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| Sequences |
Amino acid
FASTA format
|
0001 MARTTPIARY RNIGISAHID AGKTTTTERI LFYTGVNHKI GEVHDGAATM DWMEQEQERG ITITSAATTA
0071 FWSGMAKQYE PHRINIIDTP GHVDFTIEVE RSMRVLDGAV MVYCAVGGVQ PQSETVWRQA NKYKVPRIAF
0141 VNKMDRMGAN FLKVVNQIKT RLGANPVPLQ LAIGAEEHFT GVVDLVKMKA INWNDADQGV TFEYEDIPAD
0211 MVELANEWHQ NLIESAAEAS EELMEKYLGG EELTEAEIKG ALRQRVLNNE IILVTCGSAF KNKGVQAMLD
0281 AVIDYLPSPV DVPAINGILD DGKDTPAERH ASDDEPFSAL AFKIATDPFV GNLTFFRVYS GVVNSGDTVL
0351 NSVKAARERF GRIVQMHANK REEIKEVRAG DIAAAIGLKD VTTGDTLCDP DAPIILERME FPEPVISIAV
0421 EPKTKADQEK MGLALGRLAK EDPSFRVWTD EESNQTIIAG MGELHLDIIV DRMKREFNVE ANVGKPQVAY
0491 RETIRQKVTD VEGKHAKQSG GRGQYGHVVI DMYPLEPGSN PKGYEFINDI KGGVIPGEYI PAVDKGIQEQ
0561 LKAGPLAGYP VVDMGIRLHF GSYHDVDSSE LAFKLAASIA FKEGFKKAKP VLLEPIMKVE VETPEENTGD
0631 VIGDLSRRRG MLKGQESEVT GVKIHAEVPL SEMFGYATQL RSLTKGRASY TMEFLKYDEA PSNVAQAVIE
0701 ARGK
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gttatccctt cggagtttta gtcaccaggc
-070 gggcgcttcc agtaagcagc ccgctttggg ctacttaaat tgaacgccta aaagataaac gaggaaacaa
0001 atggctcgta caacacccat cgcacgctac cgtaacatcg gtatcagtgc gcacatcgac gccggtaaaa
0071 ccactactac cgaacgtatt ctgttctaca ccggtgtaaa ccataaaatc ggtgaagttc atgacggcgc
0141 tgcaaccatg gactggatgg agcaggagca ggaacgtggt attaccatca cttccgctgc gactactgca
0211 ttctggtctg gtatggctaa gcagtatgag ccgcatcgca tcaacatcat cgacaccccg gggcacgttg
0281 acttcacaat cgaagtagaa cgttccatgc gtgttctcga tggtgcggta atggtttact gcgcagttgg
0351 tggtgttcag ccgcagtctg aaaccgtatg gcgtcaggca aacaaatata aagttccgcg cattgcgttc
0421 gttaacaaaa tggaccgcat gggtgcgaac ttcctgaaag ttgttaacca gatcaaaacc cgtctgggcg
0491 cgaacccggt tccgctgcag ctggcgattg gtgctgaaga acatttcacc ggtgttgttg acctggtgaa
0561 aatgaaagct atcaactgga acgacgctga ccagggcgta accttcgaat acgaagatat cccggcagac
0631 atggttgaac tggctaacga atggcaccag aacctgatcg aatccgcagc tgaagcttct gaagagctga
0701 tggaaaaata cctgggtggt gaagaactga ctgaagcaga aatcaaaggt gctctgcgtc agcgcgttct
0771 gaacaacgaa atcatcctgg taacctgtgg ttctgcgttc aagaacaaag gtgttcaggc gatgctggat
0841 gcggtaattg attacctgcc atccccggtt gacgtacctg cgatcaacgg tatcctggac gacggtaaag
0911 acactccggc tgaacgtcac gcaagtgatg acgagccgtt ctctgcactg gcgttcaaaa tcgctaccga
0981 cccgtttgtt ggtaacctga ccttcttccg tgtttactcc ggtgtggtta actctggtga taccgtactg
1051 aactccgtga aagctgcacg tgagcgtttc ggtcgtatcg ttcagatgca cgctaacaaa cgtgaagaga
1121 tcaaagaagt tcgcgcgggc gacatcgctg ctgctatcgg tctgaaagac gtaaccactg gtgacaccct
1191 gtgtgacccg gatgcgccga tcattctgga acgtatggaa ttccctgagc cggtaatctc catcgcagtt
1261 gaaccgaaaa ccaaagctga ccaggaaaaa atgggtctgg ctctgggccg tctggctaaa gaagacccgt
1331 ctttccgtgt atggactgac gaagaatcta accagaccat catcgcgggt atgggcgaac tgcacctcga
1401 catcatcgtt gaccgtatga agcgtgaatt caacgttgaa gcgaacgtag gtaaaccgca ggttgcttac
1471 cgtgaaacta tccgccagaa agttaccgat gttgaaggta aacacgcgaa acagtctggt ggtcgtggtc
1541 agtatggtca tgttgttatc gacatgtacc cgctggagcc gggttcaaac ccgaaaggct acgagttcat
1611 caacgacatt aaaggtggtg taatccctgg cgaatacatc ccggccgttg ataaaggtat ccaggaacag
1681 ctgaaagcag gtccgctggc aggctacccg gtagtagaca tgggtattcg tctgcacttc ggttcttacc
1751 atgacgttga ctcctctgaa ctggcgttta aactggctgc ttctatcgcc tttaaagaag gctttaagaa
1821 agcgaaacca gttctgcttg agccgatcat gaaggttgaa gtagaaactc cggaagagaa caccggtgac
1891 gttatcggtg acttgagccg tcgtcgtggt atgctcaaag gtcaggaatc tgaagttact ggcgttaaga
1961 tccacgctga agtaccgctg tctgaaatgt tcggatacgc aactcagctg cgttctctga ccaaaggtcg
2031 tgcatcatac actatggaat tcctgaagta tgatgaagcg ccgagtaacg ttgctcaggc cgtaattgaa
2101 gcccgtggta aataagccta agggttaata ccaaagtccc gtgctctctc ctgaagggga gagcactata
2171 gtaaggaata tagccgtgtc taaagaaaaa tttgaacgta caaaa
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