MG1655
W3110
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Gene Report : fusA
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Bacteriol. 1994;176(1):123-9.
In vivo selection of conditional-lethal mutations in the gene encoding elongation factor G of Escherichia coli.
Hou Y, Lin YP, Sharer JD, March PE. ( 8282687 )
     Deletion OCL22-6 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  fusA
Related strains
     Strains Search Search strains by gene name:  fusA   Search strains by all related name:  fusA b3340 ECK3327 f704 far fus JW3302

General information  (Go to Linear View:)
 Gene Name fusA  
 Alternative name b3340,ECK3327,f704,far,fus,JW3302  
 Location, Length 3,469,422 - 3,471,536 (  -  ) ;   74.78 min ; 2115 (bp) ,   704 (aa) Go to Linear View
 Product protein chain elongation factor EF-G, GTP-binding  
 Operon Name rpsLG-fusA-tufA  
 Note GTP-binding protein chain elongation factor EF-G; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006412 - translation  
 Function factor; Proteins - translation and modification  
 Gene Ontology GO:0000166; nucleotide binding ( fusA )
GO:0003746; translation elongation factor activity ( fusA )
GO:0003924; GTPase activity ( fusA )
GO:0005515; protein binding ( fusA )
GO:0005525; GTP binding ( fusA )
GO:0005737; cytoplasm ( fusA )
GO:0006412; translation ( fusA )
GO:0006414; translational elongation ( fusA )
 PID 1789738  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene fusA ( EG10360 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) EFG_ECOLI ( P02996 ; Q9F439 )
    -  Protein name Elongation factor G  
    -  Synonyms EF-G  
    -  Gene name OrderedLocusNames=b3340, c4112, z4698, ECs4191;  

 Linear View (Whole Mode)
View Location
   3460.0  –  3485.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP fusA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Ribosomal Elongation Factor G  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Elongation factor G (EF-G)  
         PSI-BLAST Elongation factor G
    nr     (database updated : 2007.02.20 )
         BLAST elongation factor EF-2 [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    ATP_GTP_A    EFACTOR_GTP     

Other Cross-Reference
    COG COG0480J 
    EcoCyc fusA 

MMBR References
J Bacteriol. 1994;176:123-129
In vivo selection of conditional-lethal mutations in the gene encoding elongation factor G of Escherichia coli.
Hou, Y., Y.-P. Lin, J. D. Sharer, and P. E. March.
Biochem Biophys Res Commun. 1971;42(3):441-4.
G factor mutants of Escherichia coli: map location and properties.
Kuwano M, Schlessinger D. ( 5542892 )
J Biol Chem. 1980;255:4660-4666
DNA sequences from the str operon of Escherichia coli.
Post, L., and M. Nomura.
Nucleic Acids Res. 1984;12:2181-2192
The nucleotide sequence of the Escherichia coli fus gene, coding for elongation factor G.
Zengel, J. M., R. H. Archer, and L. Lindahl.

Sequences
Amino acid
FASTA format
0001 MARTTPIARY RNIGISAHID AGKTTTTERI LFYTGVNHKI GEVHDGAATM DWMEQEQERG ITITSAATTA 
0071 FWSGMAKQYE PHRINIIDTP GHVDFTIEVE RSMRVLDGAV MVYCAVGGVQ PQSETVWRQA NKYKVPRIAF 
0141 VNKMDRMGAN FLKVVNQIKT RLGANPVPLQ LAIGAEEHFT GVVDLVKMKA INWNDADQGV TFEYEDIPAD 
0211 MVELANEWHQ NLIESAAEAS EELMEKYLGG EELTEAEIKG ALRQRVLNNE IILVTCGSAF KNKGVQAMLD 
0281 AVIDYLPSPV DVPAINGILD DGKDTPAERH ASDDEPFSAL AFKIATDPFV GNLTFFRVYS GVVNSGDTVL 
0351 NSVKAARERF GRIVQMHANK REEIKEVRAG DIAAAIGLKD VTTGDTLCDP DAPIILERME FPEPVISIAV 
0421 EPKTKADQEK MGLALGRLAK EDPSFRVWTD EESNQTIIAG MGELHLDIIV DRMKREFNVE ANVGKPQVAY 
0491 RETIRQKVTD VEGKHAKQSG GRGQYGHVVI DMYPLEPGSN PKGYEFINDI KGGVIPGEYI PAVDKGIQEQ 
0561 LKAGPLAGYP VVDMGIRLHF GSYHDVDSSE LAFKLAASIA FKEGFKKAKP VLLEPIMKVE VETPEENTGD 
0631 VIGDLSRRRG MLKGQESEVT GVKIHAEVPL SEMFGYATQL RSLTKGRASY TMEFLKYDEA PSNVAQAVIE 
0701 ARGK

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             gttatccctt cggagtttta gtcaccaggc 
-070 gggcgcttcc agtaagcagc ccgctttggg ctacttaaat tgaacgccta aaagataaac gaggaaacaa 
0001 atggctcgta caacacccat cgcacgctac cgtaacatcg gtatcagtgc gcacatcgac gccggtaaaa 
0071 ccactactac cgaacgtatt ctgttctaca ccggtgtaaa ccataaaatc ggtgaagttc atgacggcgc 
0141 tgcaaccatg gactggatgg agcaggagca ggaacgtggt attaccatca cttccgctgc gactactgca 
0211 ttctggtctg gtatggctaa gcagtatgag ccgcatcgca tcaacatcat cgacaccccg gggcacgttg 
0281 acttcacaat cgaagtagaa cgttccatgc gtgttctcga tggtgcggta atggtttact gcgcagttgg 
0351 tggtgttcag ccgcagtctg aaaccgtatg gcgtcaggca aacaaatata aagttccgcg cattgcgttc 
0421 gttaacaaaa tggaccgcat gggtgcgaac ttcctgaaag ttgttaacca gatcaaaacc cgtctgggcg 
0491 cgaacccggt tccgctgcag ctggcgattg gtgctgaaga acatttcacc ggtgttgttg acctggtgaa 
0561 aatgaaagct atcaactgga acgacgctga ccagggcgta accttcgaat acgaagatat cccggcagac 
0631 atggttgaac tggctaacga atggcaccag aacctgatcg aatccgcagc tgaagcttct gaagagctga 
0701 tggaaaaata cctgggtggt gaagaactga ctgaagcaga aatcaaaggt gctctgcgtc agcgcgttct 
0771 gaacaacgaa atcatcctgg taacctgtgg ttctgcgttc aagaacaaag gtgttcaggc gatgctggat 
0841 gcggtaattg attacctgcc atccccggtt gacgtacctg cgatcaacgg tatcctggac gacggtaaag 
0911 acactccggc tgaacgtcac gcaagtgatg acgagccgtt ctctgcactg gcgttcaaaa tcgctaccga 
0981 cccgtttgtt ggtaacctga ccttcttccg tgtttactcc ggtgtggtta actctggtga taccgtactg 
1051 aactccgtga aagctgcacg tgagcgtttc ggtcgtatcg ttcagatgca cgctaacaaa cgtgaagaga 
1121 tcaaagaagt tcgcgcgggc gacatcgctg ctgctatcgg tctgaaagac gtaaccactg gtgacaccct 
1191 gtgtgacccg gatgcgccga tcattctgga acgtatggaa ttccctgagc cggtaatctc catcgcagtt 
1261 gaaccgaaaa ccaaagctga ccaggaaaaa atgggtctgg ctctgggccg tctggctaaa gaagacccgt 
1331 ctttccgtgt atggactgac gaagaatcta accagaccat catcgcgggt atgggcgaac tgcacctcga 
1401 catcatcgtt gaccgtatga agcgtgaatt caacgttgaa gcgaacgtag gtaaaccgca ggttgcttac 
1471 cgtgaaacta tccgccagaa agttaccgat gttgaaggta aacacgcgaa acagtctggt ggtcgtggtc 
1541 agtatggtca tgttgttatc gacatgtacc cgctggagcc gggttcaaac ccgaaaggct acgagttcat 
1611 caacgacatt aaaggtggtg taatccctgg cgaatacatc ccggccgttg ataaaggtat ccaggaacag 
1681 ctgaaagcag gtccgctggc aggctacccg gtagtagaca tgggtattcg tctgcacttc ggttcttacc 
1751 atgacgttga ctcctctgaa ctggcgttta aactggctgc ttctatcgcc tttaaagaag gctttaagaa 
1821 agcgaaacca gttctgcttg agccgatcat gaaggttgaa gtagaaactc cggaagagaa caccggtgac 
1891 gttatcggtg acttgagccg tcgtcgtggt atgctcaaag gtcaggaatc tgaagttact ggcgttaaga 
1961 tccacgctga agtaccgctg tctgaaatgt tcggatacgc aactcagctg cgttctctga ccaaaggtcg 
2031 tgcatcatac actatggaat tcctgaagta tgatgaagcg ccgagtaacg ttgctcaggc cgtaattgaa 
2101 gcccgtggta aataagccta agggttaata ccaaagtccc gtgctctctc ctgaagggga gagcactata 
2171 gtaaggaata tagccgtgtc taaagaaaaa tttgaacgta caaaa