MG1655
W3110
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Gene Report : tufA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Mol Gen Genet. 1999;260(6):603-7.
Either of the chromosomal tuf genes of E. coli K-12 can be deleted without loss of cell viability.
Zuurmond AM, Rundlof AK, Kraal B. ( 9928940 )
     Deletion OCL22 (D)  ,  OCL22-6 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  tufA
Related strains
     Strains Search Search strains by gene name:  tufA   Search strains by all related name:  tufA b3339 ECK3326 f394 JW3301 kirT pulT

General information  (Go to Linear View:)
 Gene Name tufA  
 Alternative name b3339,ECK3326,f394,JW3301,kirT,pulT  
 Location, Length 3,468,167 - 3,469,351 (  -  ) ;   74.75 min ; 1185 (bp) ,   394 (aa) Go to Linear View
 Product protein chain elongation factor EF-Tu (duplicate of tufB)  
 Operon Name rpsLG-fusA-tufA  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006412 - translation; GO_process: GO:0006970 - response to osmotic stress  
 Function factor; Proteins - translation and modification  
 Gene Ontology GO:0000166; nucleotide binding ( tufA )
GO:0003746; translation elongation factor activity ( tufA )
GO:0003924; GTPase activity ( tufA )
GO:0005515; protein binding ( tufA )
GO:0005525; GTP binding ( tufA )
GO:0005622; intracellular ( tufA )
GO:0005737; cytoplasm ( tufA )
GO:0005886; plasma membrane ( tufA )
GO:0006412; translation ( tufA )
GO:0006414; translational elongation ( tufA )
GO:0016020; membrane ( tufA )
GO:0046677; response to antibiotic ( tufA )
 PID 1789737  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene tufA ( EG11036 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) EFTU_ECOLI ( P02990 ; O68929; Q8X4S9; Q8XED3 )
    -  Protein name Elongation factor Tu  
    -  Synonyms EF-Tu, P-43  
    -  Gene name Name=tufB; OrderedLocusNames=b3980, c4935, z5553, ECs4903;  

 Linear View (Whole Mode)
View Location
   3455.0  –  3480.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP tufA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain G, Whole, Unmodified, Ef-Tu(Elongation Factor Tu).  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Elongation factor Tu  
         PSI-BLAST Elongation factor Tu-B
    nr     (database updated : 2007.02.20 )
         BLAST elongation factor Tu [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    ATP_GTP_A    EFACTOR_GTP     

Other Cross-Reference
    COG COG0050J 
    EcoCyc tufA 

MMBR References
J Biol Chem. 1996;271:22321-22325
Interplay of methionine tRNAs with translation elongation factor Tu and translation initiation factor 2 in Escherichia coli.
Guillon, J. M., S. Heiss, J. Soutourina, Y. Mechulam, S. Laalami, M. Grunberg-Manago, and S. Blanquet.
Eur J Biochem. 1980;108:507-526
The complete amino-acid sequence of elongation factor Tu from Escherichia coli.
Jones, M. D., T. E. Petersen, K. M. Nielsen, S. Magnusson, L. Sottrup-Jensen, K. Gausing, and B. F. Clark.
Biochem Cell Biol. 1995;73(11-12):1167-77.
Antibiotic resistance mechanisms of mutant EF-Tu species in Escherichia coli.
Kraal B, Zeef LA, Mesters JR, Boon K, Vorstenbosch EL, Bosch L, Anborgh PH, Parmeggiani A, Hilgenfeld R. ( 8722034 )
Eur J Biochem. 1987;170:93-98
Molecular cloning and sequence determination of the tuf gene coding for the elongation factor Tu of Thermus thermophilus HB8.
Kushiro, M., M. Shimizu, and K. Tomita.
J Biol Chem. 1981;256:8102-8109
The amino acid sequence of elongation factor Tu of Escherichia coli. The complete sequence.
Laursen, R. A., J. J. L'Italien, S. Nagarkatti, and D. L. Miller.
Biochem Biophys Res Commun. 1996;225(3):961-7.
Translational activities of EF-Tu [G222D] which cannot be reconciled with the classical scheme of the polypeptide chain elongation cycle.
Talens A, Boon K, Kraal B, Bosch L. ( 8780718 )
Proc Natl Acad Sci U S A. 1978;75:4470-4473
Mutants of Escherichia coli altered in both genes coding for the elongation factor Tu.
Van de Klundert, J. A., P. H. Van der Meide, P. Van de Putte, and L. Bosch.
FEBS Lett. 1982;139(2):325-30.
Regulation of the expression of tufA and tufB, the two genes coding for the elongation factor EF-Tu in Escherichia coli.
van der Meide PH, Vijgenboom E, Dicke M, Bosch L. ( 7042394 )
J Biol Chem. 1996;271:20406-20411
Mapping Escherichia coli elongation factor Tu residues involved in binding of aminoacyl-tRNA.
Wiborg, O., C. Andersen, C. R. Knudsen, B. F. C. Clark, and J. Nyborg.
Gene. 1980;12(1-2):25-31.
The nucleotide sequence of the cloned tufA gene of Escherichia coli.
Yokota T, Sugisaki H, Takanami M, Kaziro Y. ( 7011903 )
Mol Gen Genet. 1982;185(3):487-92.
A secondary promoter for elongation factor Tu synthesis in the str ribosomal protein operon of Escherichia coli.
Zengel JM, Lindahl L. ( 6285146 )
J Bacteriol. 1982;149(2):548-53.
Evidence for an internal promoter preceding tufA in the str operon of Escherichia coli.
An G, Lee JS, Friesen JD. ( 6276361 )

Sequences
Amino acid
FASTA format
0001 MSKEKFERTK PHVNVGTIGH VDHGKTTLTA AITTVLAKTY GGAARAFDQI DNAPEEKARG ITINTSHVEY 
0071 DTPTRHYAHV DCPGHADYVK NMITGAAQMD GAILVVAATD GPMPQTREHI LLGRQVGVPY IIVFLNKCDM 
0141 VDDEELLELV EMEVRELLSQ YDFPGDDTPI VRGSALKALE GDAEWEAKIL ELAGFLDSYI PEPERAIDKP 
0211 FLLPIEDVFS ISGRGTVVTG RVERGIIKVG EEVEIVGIKE TQKSTCTGVE MFRKLLDEGR AGENVGVLLR 
0281 GIKREEIERG QVLAKPGTIK PHTKFESEVY ILSKDEGGRH TPFFKGYRPQ FYFRTTDVTG TIELPEGVEM 
0351 VMPGDNIKMV VTLIHPIAMD DGLRFAIREG GRTVGAGVVA KVLG

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             caggccgtaa ttgaagcccg tggtaaataa 
-070 gcctaagggt taataccaaa gtcccgtgct ctctcctgaa ggggagagca ctatagtaag gaatatagcc 
0001 gtgtctaaag aaaaatttga acgtacaaaa ccgcacgtta acgttggtac tatcggccac gttgaccacg 
0071 gtaaaactac tctgaccgct gcaatcacca ccgtactggc taaaacctac ggcggtgctg ctcgtgcatt 
0141 cgaccagatc gataacgcgc cggaagaaaa agctcgtggt atcaccatca acacttctca cgttgaatac 
0211 gacaccccga cccgtcacta cgcacacgta gactgcccgg ggcacgccga ctatgttaaa aacatgatca 
0281 ccggtgctgc tcagatggac ggcgcgatcc tggtagttgc tgcgactgac ggcccgatgc cgcagactcg 
0351 tgagcacatc ctgctgggtc gtcaggtagg cgttccgtac atcatcgtgt tcctgaacaa atgcgacatg 
0421 gttgatgacg aagagctgct ggaactggtt gaaatggaag ttcgtgaact tctgtctcag tacgacttcc 
0491 cgggcgacga cactccgatc gttcgtggtt ctgctctgaa agcgctggaa ggcgacgcag agtgggaagc 
0561 gaaaatcctg gaactggctg gcttcctgga ttcttatatt ccggaaccag agcgtgcgat tgacaagccg 
0631 ttcctgctgc cgatcgaaga cgtattctcc atctccggtc gtggtaccgt tgttaccggt cgtgtagaac 
0701 gcggtatcat caaagttggt gaagaagttg aaatcgttgg tatcaaagag actcagaagt ctacctgtac 
0771 tggcgttgaa atgttccgca aactgctgga cgaaggccgt gctggtgaga acgtaggtgt tctgctgcgt 
0841 ggtatcaaac gtgaagaaat cgaacgtggt caggtactgg ctaagccggg caccatcaag ccgcacacca 
0911 agttcgaatc tgaagtgtac attctgtcca aagatgaagg cggccgtcat actccgttct tcaaaggcta 
0981 ccgtccgcag ttctacttcc gtactactga cgtgactggt accatcgaac tgccggaagg cgtagagatg 
1051 gtaatgccgg gcgacaacat caaaatggtt gttaccctga tccacccgat cgcgatggac gacggtctgc 
1121 gtttcgcaat ccgtgaaggc ggccgtaccg ttggcgcggg cgttgttgct aaagttctgg gctaattaca 
1191 cgttaattag ttttgaattg aaaagggcgc ttcggcgccc tttttgcatt tgttgacggc atatcttatt 
1261 cgtactgtca gaacgaataa attat