MG1655
W3110
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Gene Report : rpsD
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCL22,23-4 (BK5-12 / AK4-12) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  rpsD
Related strains
     Strains Search Search strains by gene name:  rpsD   Search strains by all related name:  rpsD b3296 ECK3283 f206 JW3258 ramA sud

General information  (Go to Linear View:)
 Gene Name rpsD  
 Alternative name b3296,ECK3283,f206,JW3258,ramA,sud  
 Location, Length 3,439,077 - 3,439,697 (  -  ) ;   74.12 min ; 621 (bp) ,   206 (aa) Go to Linear View
 Product 30S ribosomal subunit protein S4  
 Operon Name rpsMKD-rpoA-rplQ  
 Note GO_component: GO:0005737 - cytoplasm; GO_function: GO:0003735 - structural constituent of ribosome; GO_process: GO:0006412 - translation  
 Function structural component; Ribosomal proteins - synthesis, modification  
 Gene Ontology GO:0003723 ; RNA binding ( rpsD )
GO:0003735 ; structural constituent of ribosome ( rpsD )
GO:0005515 ; protein binding ( rpsD )
GO:0005622 ; intracellular ( rpsD )
GO:0005840 ; ribosome ( rpsD )
GO:0006350 ; transcription ( rpsD )
GO:0006353 ; transcription termination ( rpsD )
GO:0006355 ; regulation of transcription, DNA-dependent ( rpsD )
GO:0006412 ; translation ( rpsD )
GO:0006417 ; regulation of translation ( rpsD )
GO:0015935 ; small ribosomal subunit ( rpsD )
GO:0019843 ; rRNA binding ( rpsD )
GO:0030529 ; ribonucleoprotein complex ( rpsD )
GO:0046677 ; response to antibiotic ( rpsD )
 PID 1789691  
 EC number
  (KEGG Pathway)   		 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene rpsD ( EG10903 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) RS4_ECOLI ( P02354 )
    -  Protein name 30S ribosomal protein S4.  
    -  Synonyms  
    -  Gene name OrderedLocusNames=b3296, c4057, z4666, ECs4161;  

 Linear View (Whole Mode)
View Location
   3430.0  –  3455.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP rpsD (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain D, Crystal Structure Of The Bacterial Ribosome From Escherichia Coli In Complex With The Antibiotic Kasugamyin At 3.5a Resolution. This File Contains The 30s Subunit Of One 70s Ribosome. The Entire Crystal Structure Contains Two 70s Ribosomes And Is  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST 30S ribosomal protein S4  
         PSI-BLAST 30S ribosomal protein S4
    nr     (database updated : 2007.02.20 )
         BLAST 30S ribosomal protein S4 [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    RIBOSOMAL_S4     

Other Cross-Reference
    COG COG0522J 
    EcoCyc rpsD 

MMBR References
Mol Gen Genet. 1973;127(1):1-18.
Electrophoretic and immunological studies on ribosomal proteins of 100 Escherichia coli revertants from streptomycin dependence.
Hasenbank R, Guthrie C, Stoffler G, Wittmann HG, Rosen L, Apirion D. ( 4589343 )
Nucleic Acids Res. 1985;13:3891-3903
Nucleotide sequence of the alpha ribosomal protein operon of Escherichia coli.
Bedwell, D. M., G. R. Davis, M. Gosink, L. Post, M. Nomura, H. Kestler, J. M. Zengel, and L. Lindahl.
J Biol Chem. 1980;255:4653-4659
DNA sequence of the promoter region for the a ribosomal protein operon in Escherichia coli.
Post, L., A. E. Arfsten, G. R. Davis, and M. Nomura.
J Biol Chem. 1979;254:10604-10606
Nucleotide sequence of the intercistronic region preceding the gene for RNA polymerase subunit a in Escherichia coli.
Post, L., and M. Nomura.
Nucleic Acids Res. 1983;11:2599-2616
The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene.
Cerretti, D. P., D. Dean, G. R. Davis, D. M. Bedwell, and M. Nomura.

Sequences
Amino acid
FASTA format 
0001 MARYLGPKLK LSRREGTDLF LKSGVRAIDT KCKIEQAPGQ HGARKPRLSD YGVQLREKQK VRRIYGVLER 
0071 QFRNYYKEAA RLKGNTGENL LALLEGRLDN VVYRMGFGAT RAEARQLVSH KAIMVNGRVV NIASYQVSPN 
0141 DVVSIREKAK KQSRVKAALE LAEQREKPTW LEVDAGKMEG TFKRKPERSD LSADINEHLI VELYSK
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             taacattact gatgtgactc cgatccctca 
-070 taacggttgt cgtccgccga aaaaacgtcg cgtataacgc ctcgttttcc aggtttgttg gagaaagaaa 
0001 atggcaagat atttgggtcc taagctcaag ctgagccgtc gtgagggcac cgacttattc cttaagtctg 
0071 gcgttcgcgc gatcgatacc aagtgtaaaa ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg 
0141 tctgtctgac tatggtgtgc agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt gctggagcgt 
0211 cagttccgta actactacaa agaagcagca cgtctgaaag gcaacaccgg tgaaaacctg ttggctctgc 
0281 tggaaggtcg tctggacaac gttgtatacc gtatgggctt cggtgccact cgtgcagaag cacgtcagct 
0351 ggttagccat aaagcaatta tggtaaacgg tcgtgttgtt aacatcgctt cttatcaggt tagtccgaat 
0421 gacgttgtaa gcattcgtga gaaagcgaag aagcagtctc gcgtgaaagc cgctctggag ctggctgagc 
0491 agcgtgaaaa gccaacctgg ctggaagttg atgctggcaa gatggaaggt acgtttaagc gtaagccgga 
0561 gcgttctgat ctgtctgcgg acattaacga acacctgatc gtcgagcttt actccaagta aagcttagta 
0631 ccaaagagag gacacaatgc agggttctgt gacagagttt ctaaaaccgc gcctggttga tatcgagcaa 
0701 gtgagttcga cgcacgccaa g