|
PEC Original Annotations
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Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
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Deletion
|
OCL22,23-4 (BK5-12 / AK4-12)
(D)
,
OCL23
(D)
,
OCL23-13
(D)
,
OCL23-2
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
trkA
|
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Related strains
|
|
Strains Search
|
Search strains by gene name:
trkA
Search strains by all related name:
trkA b3290 ECK3276 JW3251 o458 sapG
|
General information (Go to Linear View: )
|
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Gene Name
|
trkA
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|
Alternative name
|
b3290,ECK3276,JW3251,o458,sapG
|
|
Location, Length
|
3,434,540 - 3,435,916
(
+
)
;
74.03
min
;
1377
(bp) ,
458
(aa)
|
|
Product
|
NAD-binding component of TrK potassium transporter
|
|
Operon Name
|
trkA
|
|
Note
|
transport of potassium; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane
|
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Function
|
transport; Transport of small molecules: Cations
|
|
Gene Ontology
|
GO:0003824
;
catalytic activity ( trkA )
GO:0005488
;
binding ( trkA )
GO:0005886
;
plasma membrane ( trkA )
GO:0006810
;
transport ( trkA )
GO:0006811
;
ion transport ( trkA )
GO:0006813
;
potassium ion transport ( trkA )
GO:0008152
;
metabolic process ( trkA )
GO:0008324
;
cation transmembrane transporter activity ( trkA )
GO:0016020
;
membrane ( trkA )
GO:0030955
;
potassium ion binding ( trkA )
|
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PID
|
1789685
|
EC number
(KEGG Pathway)
|
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
trkA
(
EG11019
)
|
|
Number of removed
|
0 aa cleaved
|
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SWISS-PROT
(
Show details
)
|
Entry name(Acc.no)
|
TRKA_ECOLI
(
P23868
;
P77041
)
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- Protein name
|
Trk system potassium uptake protein trkA
|
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- Synonyms
|
K(+)-uptake protein trkA
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|
- Gene name
|
OrderedLocusNames=b3290, c4050, z4660, ECs4155, SF3321, S3546;
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Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
trkA
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Ktn Mja218 Crystal Structure In Complex With Nad+
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
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BLAST
|
Trk system potassium uptake protein trkA (K(+)-uptake protein trkA)
|
|
PSI-BLAST
|
Ketol-acid reductoisomerase
|
|
nr
(database updated :
2007.02.20
)
|
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BLAST
|
potassium transporter peripheral membrane component [Escherichia coli O157:H7 EDL933]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
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PROSITE
|
|
PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
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MMBR References
|
J Bacteriol. 1971;108(2):639-44.
Potassium transport loci in Escherichia coli K-12.
Epstein W, Kim BS.
(
4942756
)
|
Biochem Soc Trans. 1993;21(4):1006-10.
Multiple mechanisms, roles and controls of K+ transport in Escherichia coli.
Epstein W, Buurman E, McLaggan D, Naprstek J.
(
8131887
)
|
J Bacteriol. 1987;169(7):3138-45.
Physical mapping of the K+ transport trkA gene of Escherichia coli and overproduction of the TrkA protein.
Hamann A, Bossemeyer D, Bakker EP.
(
3298214
)
|
Nucleic Acids Res. 1984;12:5813-5821
Nucleotide sequence of the rpoA-rplQ DNA of Escherichia coli: a second regulatory binding site for protein S4?
Meek, D. W., and R. S. Hayward.
|
Mol Microbiol. 1993;9(3):533-43.
NAD+ binding to the Escherichia coli K(+)-uptake protein TrkA and sequence similarity between TrkA and domains of a family of dehydrogenases suggest a role for NAD+ in bacterial transport.
Schlosser A, Hamann A, Bossemeyer D, Schneider E, Bakker EP.
(
8412700
)
|
J Biol Chem. 1989;264:16403-16410
K+-transport protein TrkA of Escherichia coli is a peripheral membrane protein that requires other trk gene products for attachment to the cytoplasmic membrane.
Bossemeyer, D., A. Borchard, D. C. Dosch, G. L. Helmer, W. Epstein, I. R. Booth, and E. P. Bakker.
|
| Sequences |
Amino acid
FASTA format
|
0001 MKIIILGAGQ VGGTLAENLV GENNDITVVD TNGERLRTLQ DKFDLRVVQG HGSHPRVLRE AGADDADMLV
0071 AVTSSDETNM VACQVAYSLF NTPNRIARIR SPDYVRDADK LFHSDAVPID HLIAPEQLVI DNIYRLIEYP
0141 GALQVVNFAE GKVSLAVVKA YYGGPLIGNA LSTMREHMPH IDTRVAAIFR HDRPIRPQGS TIVEAGDEVF
0211 FIAASQHIRA VMSELQRLEK PYKRIMLVGG GNIGAGLARR LEKDYSVKLI ERNQQRAAEL AEKLQNTIVF
0281 FGDASDQELL AEEHIDQVDL FIAVTNDDEA NIMSAMLAKR MGAKKVMVLI QRRAYVDLVQ GSVIDIAISP
0351 QQATISALLS HVRKADIVGV SSLRRGVAEA IEAVAHGDES TSRVVGRVID EIKLPPGTII GAVVRGNDVM
0421 IANDNLRIEQ GDHVIMFLTD KKFITDVERL FQPSPFFL
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 AGAGCAACCG GGTAAACAAA ATCTACCTGG
-070 TGCCGAAGAG GGCGACGGCT TCTTTTACGC TAAGCTAATC AAAAAGTGAT GAGATAACGG GTCGCGACTG
0001 atgaaaatta tcattctggg tgccggccag gttggcggca cactggcgga aaacctggtt ggcgagaaca
0071 acgatattac tgttgtcgat accaacggtg agcgtctgcg gaccttacag gataaatttg acctgcgggt
0141 cgtgcagggg catggctctc atccacgcgt attgcgggag gcaggtgccg acgacgccga tatgctggtt
0211 gctgtaacca gttcagatga aaccaatatg gttgcctgcc aggtagccta ctcacttttc aacaccccta
0281 atcgcatcgc tcgtatccgc tcaccagact acgtgcgcga tgccgataag ctatttcatt cagatgctgt
0351 gccgattgat catctgatcg caccagagca gttggttatc gataatattt accgactgat tgagtatccc
0421 ggcgcattgc aggtggtgaa cttcgctgag ggtaaagtca gcctggctgt ggttaaagcc tattatggcg
0491 gcccgctgat tggtaatgca ctttcgacca tgcgcgaaca tatgccacat atcgatactc gtgtggcagc
0561 aattttccgc cacgatcgcc ccattcgtcc gcaaggttcg accattgttg aagctggtga tgaagtgttc
0631 tttattgccg cttcacagca tatccgcgcg gtgatgagtg aattacagcg actggaaaaa ccgtataagc
0701 ggatcatgct ggttggtggc ggtaatatcg gtgcagggct ggcgcgtcgt ctggaaaaag attacagcgt
0771 taaactcatc gaacgtaatc agcagcgcgc tgccgaactg gcggaaaagt tacagaatac gatcgtcttt
0841 tttggtgatg cgtcggatca agaactactg gccgaagaac atatcgatca agttgatctg tttattgccg
0911 tcaccaacga tgacgaggcc aatatcatgt ccgccatgct tgccaaacgt atgggtgcga aaaaggtgat
0981 ggtattgatc cagcgtcgcg cttatgtgga tctggttcag gggagcgtta tcgatattgc gatttcacca
1051 caacaagcaa ctatttctgc gttgcttagc catgtgcgaa aagcagatat tgttggtgtt tcctcattgc
1121 gccgcggcgt agcagaagct attgaagccg ttgctcacgg tgatgaaagc acctcacgcg ttgtcggcag
1191 agtcattgac gaaatcaagc taccgccagg aacgattatt ggagcggtgg tacgtggaaa cgacgtgatg
1261 attgccaatg acaatctgcg cattgagcaa ggcgatcacg taattatgtt cctcacagat aaaaagttta
1331 ttaccgacgt cgaaagactc ttccagccaa gccctttctt cttgtaaTTA ATAAGGCGTC TAATGACGCC
1401 TTATTATTTC CCTTTGATTA TCAAGGATTA ATTAAATTCA TTCCTGGCAG GAAAATGGCT TAACATTTGT
1471 TAGACTT
|
