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PEC Original Annotations
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|
Essentiality
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Class
|
non-essential
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|
References (PMID)
|
Mol Gen Genet. 1991;230(1-2):230-40.
Molecular analysis and nucleotide sequence of the envCD operon of Escherichia coli.
Klein JR, Henrich B, Plapp R.
(
1720861
)
|
|
|
Deletion
|
OCL23
(D)
,
OCL23-15
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
acrF
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
acrF
Search strains by all related name:
acrF b3266 ECK3253 envD JW3234 o1034
|
General information (Go to Linear View: )
|
|
Gene Name
|
acrF
|
|
Alternative name
|
b3266,ECK3253,envD,JW3234,o1034
|
|
Location, Length
|
3,413,055 - 3,416,159
(
+
)
;
73.56
min
;
3105
(bp) ,
1034
(aa)
|
|
Product
|
multidrug efflux system protein
|
|
Operon Name
|
acrEF
|
|
Note
|
integral transmembrane protein; acridine resistance; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0042493 - response to drug
|
|
Function
|
membrane; Drug/analog sensitivity
|
|
Gene Ontology
|
GO:0005215
;
transporter activity ( acrF )
GO:0005886
;
plasma membrane ( acrF )
GO:0006810
;
transport ( acrF )
GO:0007049
;
cell cycle ( acrF )
GO:0016020
;
membrane ( acrF )
GO:0016021
;
integral to membrane ( acrF )
GO:0051301
;
cell division ( acrF )
|
|
PID
|
1789666
|
EC number
(KEGG Pathway)
|
|
|
SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
ACRF_ECOLI
(
P24181
)
|
|
- Protein name
|
Acriflavine resistance protein F
|
|
- Synonyms
|
Protein envD
|
|
- Gene name
|
Name=acrF; Synonyms=envD; OrderedLocusNames=b3266;
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Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
acrF
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Structural Basis Of The Multiple Binding Capacity Of The Acrb Multidrug Efflux Pump
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Acriflavine resistance protein F
|
|
PSI-BLAST
|
Multidrug resistance protein mexB
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
multidrug efflux system protein [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
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Pfam
|
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PROSITE
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PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
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MMBR References
|
Mol Gen Genet. 1991;230(1-2):230-40.
Molecular analysis and nucleotide sequence of the envCD operon of Escherichia coli.
Klein JR, Henrich B, Plapp R.
(
1720861
)
|
J Bacteriol. 1992;174(11):3828-9.
Locations of the envCD genes on the physical map of the Escherichia coli chromosome.
Klein JR, Plapp R.
(
1592834
)
|
Trends Biochem Sci. 1994;19(3):119-23.
Multidrug resistance pumps in bacteria: variations on a theme.
Lewis K.
(
8203018
)
|
J Bacteriol. 1993;175:6299-6313
Molecular cloning and characterization of acrA and acrE genes of Escherichia coli.
Ma, D., D. N. Cook, M. Alberti, N. G. Pon, H. Nikaido, and J. E. Hearst.
|
| Sequences |
Amino acid
FASTA format
|
0001 MANFFIRRPI FAWVLAIILM MAGALAILQL PVAQYPTIAP PAVSVSANYP GADAQTVQDT VTQVIEQNMN
0071 GIDNLMYMSS TSDSAGSVTI TLTFQSGTDP DIAQVQVQNK LQLATPLLPQ EVQQQGISVE KSSSSYLMVA
0141 GFVSDNPGTT QDDISDYVAS NVKDTLSRLN GVGDVQLFGA QYAMRIWLDA DLLNKYKLTP VDVINQLKVQ
0211 NDQIAAGQLG GTPALPGQQL NASIIAQTRF KNPEEFGKVT LRVNSDGSVV RLKDVARVEL GGENYNVIAR
0281 INGKPAAGLG IKLATGANAL DTAKAIKAKL AELQPFFPQG MKVLYPYDTT PFVQLSIHEV VKTLFEAIML
0351 VFLVMYLFLQ NMRATLIPTI AVPVVLLGTF AILAAFGYSI NTLTMFGMVL AIGLLVDDAI VVVENVERVM
0421 MEDKLPPKEA TEKSMSQIQG ALVGIAMVLS AVFIPMAFFG GSTGAIYRQF SITIVSAMAL SVLVALILTP
0491 ALCATLLKPV SAEHHENKGG FFGWFNTTFD HSVNHYTNSV GKILGSTGRY LLIYALIVAG MVVLFLRLPS
0561 SFLPEEDQGV FLTMIQLPAG ATQERTQKVL DQVTDYYLKN EKANVESVFT VNGFSFSGQA QNAGMAFVSL
0631 KPWEERNGDE NSAEAVIHRA KMELGKIRDG FVIPFNMPAI VELGTATGFD FELIDQAGLG HDALTQARNQ
0701 LLGMAAQHPA SLVSVRPNGL EDTAQFKLEV DQEKAQALGV SLSDINQTIS TALGGTYVND FIDRGRVKKL
0771 YVQADAKFRM LPEDVDKLYV RSANGEMVPF SAFTTSHWVY GSPRLERYNG LPSMEIQGEA APGTSSGDAM
0841 ALMENLASKL PAGIGYDWTG MSYQERLSGN QAPALVAISF VVVFLCLAAL YESWSIPVSV MLVVPLGIVG
0911 VLLAATLFNQ KNDVYFMVGL LTTIGLSAKN AILIVEFAKD LMEKEGKGVV EATLMAVRMR LRPILMTSLA
0981 FILGVLPLAI SNGAGSGAQN AVGIGVMGGM VSATLLAIFF VPVFFVVIRR CFKG
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 AAGTCATTGT CAGCGGCCTG CAAAAAGCGC
-070 GTCCGGGAGA GCAGGTTAAA GCCACTACCG ATACCCCCGC AGATACTGCA TCGAAGTAAG GTAATCTGAC
0001 atggcaaact tttttattcg acgaccgata tttgcatggg tgctggccat tattctgatg atggcgggcg
0071 cactggcgat cctacaattg cccgtcgctc agtatccaac aattgcaccg cctgcggttt ctgtttcagc
0141 aaactatccg ggcgctgatg cgcagaccgt gcaggatacg gtgacgcagg ttatcgaaca gaatatgaac
0211 ggtatcgata acctgatgta tatgtcctcc accagcgatt ccgccggtag cgtgacaatt acccttacct
0281 tccagtccgg gaccgatcct gatatcgcgc aagtgcaggt gcagaacaaa ctccagctcg ccacgccgtt
0351 gctgccgcag gaggttcagc agcaggggat cagtgttgaa aagtccagta gcagctattt gatggtggcg
0421 ggctttgtct ctgataaccc aggcaccaca caggacgata tctcggacta tgtggcctct aacgttaaag
0491 atacgcttag ccgtctgaat ggcgtcggtg acgtacagct tttcggcgca cagtatgcga tgcgtatctg
0561 gctggatgcc gatctgctaa acaaatataa actgacaccg gttgatgtga ttaaccagtt gaaggtacag
0631 aacgatcaga tcgctgccgg acagttgggc ggaacgccag cgttaccagg gcaacaattg aacgcctcga
0701 ttattgctca gacgcggttt aaaaatccgg aagaattcgg caaagtgacc ctgcgcgtaa acagtgacgg
0771 ctcggtggta cgcctgaaag atgtcgcacg ggttgaactt ggcggtgaaa actataacgt tatcgctcgt
0841 atcaacggaa aaccggcggc gggcctgggg attaagctgg caaccggcgc gaatgctctc gataccgcga
0911 aagccattaa ggcaaaactg gcggaattac agccattctt cccgcaggga atgaaggttc tctaccctta
0981 tgacaccacg ccattcgtcc agctttctat tcacgaagtg gtaaaaacgc tgttcgaagc cattatgctg
1051 gtgttcctgg tgatgtatct gttcttgcag aatatgcgag caacgctgat ccccaccatt gcggtacccg
1121 tggtgttgtt agggacgttt gccatcctcg ccgcttttgg ttactccatc aacacactaa cgatgttcgg
1191 gatggtgctt gccatcgggc tgctcgtcga tgatgcgata gtggtggtgg agaacgtcga gcgcgtgatg
1261 atggaggata agctcccgcc aaaagaagcg acggaaaaat cgatgtcgca aattcagggc gcactggtgg
1331 gtatcgcgat ggtgctgtca gcggtattta ttccgatggc attcttcggc ggttctactg gggcaattta
1401 tcgccagttc tctatcacca tcgtttcggc aatggcgctt tctgttctgg tggcattgat tcttacccct
1471 gcgttatgtg caacgctgct taaacccgtc tctgctgagc atcacgaaaa taagggcggt ttcttcggtt
1541 ggtttaatac caccttcgat catagcgtta accactacac caacagcgtc ggcaaaatcc tcggatccac
1611 aggacgatat ttactgatct atgcgctgat tgttgcagga atggtggtgt tgtttttacg tcttccgtct
1681 tccttcttac ctgaagagga tcagggtgtc tttctgacca tgattcagtt acccgctggc gcgacgcaag
1751 agcggacgca aaaagtgttg gatcaagtta cggattacta tctgaagaac gagaaagcga acgttgaaag
1821 tgtctttacg gttaacggct ttagcttcag cggccaggca caaaacgccg gtatggcctt cgtcagtctg
1891 aaaccgtggg aagagcgtaa tggtgacgaa aacagtgcgg aagcggtaat ccatcgtgcc aaaatggaat
1961 tgggcaagat ccgcgacggt tttgtcattc cattcaatat gccagccatt gttgaactgg gcacggcaac
2031 gggtttcgac tttgagttaa ttgatcaggc tgggctgggt cacgatgccc taacccaggc ccgtaaccag
2101 ttgcttggta tggcggcgca acatcctgcc agcttagtca gcgtgcgccc taatggcctg gaagacaccg
2171 cgcagtttaa actggaagtt gaccaggaaa aggcgcaggc attaggtgtt tcactttctg acatcaatca
2241 gaccatttca acggcgctgg gtgggactta cgttaacgac ttcatcgacc gtggccgcgt gaaaaagttg
2311 tatgttcagg cggatgccaa attccgtatg ctgccagaag atgtcgataa actttatgtc cgcagcgcca
2381 acggcgaaat ggtgccattc tcggccttta ccacttcaca ttgggtgtat ggctctccgc gactggaacg
2451 ctacaacggt ctgccgtcaa tggagattca gggggaagcc gcgccaggaa ccagttccgg cgatgccatg
2521 gcgttgatgg aaaaccttgc gtcaaaatta cctgcgggca ttggttatga ctggacgggt atgtcgtatc
2591 aggaacgctt atcgggaaac caggctcccg ctctggtagc aatttccttt gtggttgttt tcctgtgcct
2661 tgctgcactc tatgaaagct ggtcaattcc tgtctcggtt atgttggtag tgccgttagg gattgtcggc
2731 gtgctgctgg cggcgacact ctttaatcaa aaaaatgacg tctactttat ggtgggcttg ctaacgacaa
2801 ttggcttgtc ggccaaaaac gctattttga tcgttgagtt cgctaaagat ctcatggaga aagagggtaa
2871 aggtgttgtt gaagcgacac tgatggcagt acgtatgcgt ctgcgtccta tcctgatgac ctctctcgcc
2941 tttattctcg gcgtattacc gctagctatc agtaacggtg ccggcagtgg cgcgcagaac gctgtgggta
3011 tcggggtaat gggaggaatg gtctctgcaa cgttgctggc aatcttcttc gtaccggtgt tctttgtggt
3081 gatccgccgt tgctttaaag gataaATCAG AAACATAAAG GCGCTTTCGG GTGCCTTTAT TATTTCCAGT
3151 GAAACCCATA AAAATTAATA AGATATTCTT CTGCTCACTC TTTAAAAGCT TTCTA
|
