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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Bacteriol. 1995;177(14):4194-7.
MurA (MurZ), the enzyme that catalyzes the first committed step in peptidoglycan biosynthesis, is essential in Escherichia coli.
Brown ED, Vivas EI, Walsh CT, Kolter R.
(
7608103
)
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Deletion
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OCL28,29-1
(D)
,
OCL29,30-6 (AK4-11 / BK5-11)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
murA
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Related strains
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Strains Search
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Search strains by gene name:
murA
Search strains by all related name:
murA b3189 ECK3178 f419 JW3156 mrbA murZ
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General information (Go to Linear View: )
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Gene Name
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murA
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Alternative name
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b3189,ECK3178,f419,JW3156,mrbA,murZ
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Location, Length
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3,333,257 - 3,334,516
(
-
)
;
71.84
min
;
1260
(bp) ,
419
(aa)
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Product
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UDP-N-acetylglucosamine 1-carboxyvinyltransferase
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Operon Name
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murA
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Note
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first step in murein biosynthesis;UDP-N-glucosamine 1-carboxyvinyltransferase; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_process: GO:0009252 - peptidoglycan biosynthetic process
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Function
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enzyme; Murein sacculus, peptidoglycan
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Gene Ontology
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GO:0003824
;
catalytic activity ( murA )
GO:0005737
;
cytoplasm ( murA )
GO:0007047
;
cell wall organization and biogenesis ( murA )
GO:0007049
;
cell cycle ( murA )
GO:0008360
;
regulation of cell shape ( murA )
GO:0008760
;
UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity ( murA )
GO:0009252
;
peptidoglycan biosynthetic process ( murA )
GO:0016740
;
transferase activity ( murA )
GO:0016765
;
transferase activity, transferring alkyl or aryl (other than methyl) groups ( murA )
GO:0019277
;
UDP-N-acetylgalactosamine biosynthetic process ( murA )
GO:0051301
;
cell division ( murA )
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PID
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1789580
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EC number
(KEGG Pathway)
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2.5.1.7
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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murA
(
EG11358
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
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MURA_ECOLI
(
P28909
)
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- Protein name
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UDP-N-acetylglucosamine 1-carboxyvinyltransferase
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- Synonyms
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EC 2.5.1.7, Enoylpyruvate transferase, UDP-N-acetylglucosamine enolpyruvyl transferase, EPT
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- Gene name
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OrderedLocusNames=b3189, c3947, SF3229, S3447;
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MMBR References
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J Bacteriol. 1992;174:5748-5752
Cloning and sequencing of Escherichia coli murZ and purification of its product, a UDP-N-acetylglucosamine enolpyruvyl transferase.
Marquardt, J. L., D. A. Siegele, R. Kolter, and C. T. Walsh.
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J Bacteriol. 1992;174:1690-1693
Cloning and identification of the Escherichia coli murB DNA sequence, which encodes UDP-N-acetylenolpyruvoylglucosamine reductase.
Pucci, M. J., L. F. Discotto, and T. J. Dougherty.
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J Bacteriol. 1972;110(3):935-44.
Isolation and characterization of a phosphonomycin-resistant mutant of Escherichia coli K-12.
Venkateswaran PS, Wu HC.
(
4555418
)
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J Bacteriol. 1995;177:4194-4197
MurA (MurZ), the enzyme that catalyzes the first committed step in peptidoglycan biosynthesis, is essential in Escherichia coli.
Brown, E. D., E. I. Vivas, C. T. Walsh, and R. Kolter.
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| Sequences |
Amino acid
FASTA format
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0001 MDKFRVQGPT KLQGEVTISG AKNAALPILF AALLAEEPVE IQNVPKLKDV DTSMKLLSQL GAKVERNGSV
0071 HIDARDVNVF CAPYDLVKTM RASIWALGPL VARFGQGQVS LPGGCTIGAR PVDLHISGLE QLGATIKLEE
0141 GYVKASVDGR LKGAHIVMDK VSVGATVTIM CAATLAEGTT IIENAAREPE IVDTANFLIT LGAKISGQGT
0211 DRIVIEGVER LGGGVYRVLP DRIETGTFLV AAAISRGKII CRNAQPDTLD AVLAKLRDAG ADIEVGEDWI
0281 SLDMHGKRPK AVNVRTAPHP AFPTDMQAQF TLLNLVAEGT GFITETVFEN RFMHVPELSR MGAHAEIESN
0351 TVICHGVEKL SGAQVMATDL RASASLVLAG CIAEGTTVVD RIYHIDRGYE RIEDKLRALG ANIERVKGE
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 tacccctgcg gagtgggcgc gcgatcgcaa
-070 actgaacggc ttttgagcta tgggcgattc gccggtagcg gatatgaatt gttaactgag aacaaactaa
0001 atggataaat ttcgtgttca ggggccaacg aagctccagg gcgaagtcac aatttccggc gctaaaaatg
0071 ctgctctgcc tatccttttt gccgcactac tggcggaaga accggtagag atccagaacg tcccgaaact
0141 gaaagacgtc gatacatcaa tgaagctgct aagccagctg ggtgcgaaag tagaacgtaa tggttctgtg
0211 catattgatg cccgcgacgt taatgtattc tgcgcacctt acgatctggt taaaaccatg cgtgcttcta
0281 tctgggcgct ggggccgctg gtagcgcgct ttggtcaggg gcaagtttca ctacctggcg gttgtacgat
0351 cggtgcgcgt ccggttgatc tacacatttc tggcctcgaa caattaggcg cgaccatcaa actggaagaa
0421 ggttacgtta aagcttccgt cgatggtcgt ttgaaaggtg cacatatcgt gatggataaa gtcagcgttg
0491 gcgcaacggt gaccatcatg tgtgctgcaa ccctggcgga aggcaccacg attattgaaa acgcagcgcg
0561 tgaaccggaa atcgtcgata ccgcgaactt cctgattacg ctgggtgcga aaattagcgg tcagggcacc
0631 gatcgtatcg tcatcgaagg tgtggaacgt ttaggcggcg gtgtctatcg cgttctgccg gatcgtatcg
0701 aaaccggtac tttcctggtg gcggcggcga tttctcgcgg caaaattatc tgccgtaacg cgcagccaga
0771 tactctcgac gccgtgctgg cgaaactgcg tgacgctgga gcggacatcg aagtcggcga agactggatt
0841 agcctggata tgcatggcaa acgtccgaag gctgttaacg tacgtaccgc gccgcatccg gcattcccga
0911 ccgatatgca ggcccagttc acgctgttga acctggtggc agaagggacc gggtttatca ccgaaacggt
0981 ctttgaaaac cgctttatgc atgtgccaga gctgagccgt atgggcgcgc acgccgaaat cgaaagcaat
1051 accgttattt gtcacggtgt tgaaaaactt tctggcgcac aggttatggc aaccgatctg cgtgcatcag
1121 caagcctggt gctggctggc tgtattgcgg aagggacgac ggtggttgat cgtatttatc acatcgatcg
1191 tggctacgaa cgcattgaag acaaactgcg cgctttaggt gcaaatattg agcgtgtgaa aggcgaataa
1261 tcgtctgaga gctggcagcc ccgttcgcgg ggctaccgcc agaccactca ttttttcggt ttagtgtagc
1331 tacgcatcaa ctgcgttctg tcgataaact
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