MG1655
W3110
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Gene Report : dacB
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Mol Gen Genet. 1983;189(2):215-21.
Mutants of Escherichia coli defective in penicillin-insensitive murein DD-endopeptidase.
Iida K, Hirota Y, Schwarz U. ( 6343788 )
     Deletion OCL29,30-6 (AK4-11 / BK5-11) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  dacB
Related strains
     Strains Search Search strains by gene name:  dacB   Search strains by all related name:  dacB b3182 ECK3171 JW3149 o477

General information  (Go to Linear View:)
 Gene Name dacB  
 Alternative name b3182,ECK3171,JW3149,o477  
 Location, Length 3,326,985 - 3,328,418 (  +  ) ;   71.71 min ; 1434 (bp) ,   477 (aa) Go to Linear View
 Product D-alanyl-D-alanine carboxypeptidase  
 Operon Name dacB  
 Note D-alanyl-D-alanine carboxypeptidase, fraction B; penicillin-binding protein 4; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0042597 - periplasmic space; GO_process: GO:0009252 - peptidoglycan biosynthetic process; GO_process: GO:0042493 - response to drug  
 Function enzyme; Murein sacculus, peptidoglycan  
 Gene Ontology GO:0004185; serine carboxypeptidase activity ( dacB )
GO:0005515; protein binding ( dacB )
GO:0006508; proteolysis ( dacB )
GO:0007047; cell wall organization and biogenesis ( dacB )
GO:0007049; cell cycle ( dacB )
GO:0008360; regulation of cell shape ( dacB )
GO:0009002; serine-type D-Ala-D-Ala carboxypeptidase activity ( dacB )
GO:0009252; peptidoglycan biosynthetic process ( dacB )
GO:0016787; hydrolase activity ( dacB )
GO:0042597; periplasmic space ( dacB )
GO:0046677; response to antibiotic ( dacB )
GO:0051301; cell division ( dacB )
 PID 1789573  
 EC number
  (KEGG Pathway)
 
3.4.16.4  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene dacB ( EG10202 )  
    Number of removed 20 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) PBP4_ECOLI ( P24228 )
    -  Protein name Penicillin-binding protein 4 precursor  
    -  Synonyms PBP-4  
    -  Gene name Name=dacB; OrderedLocusNames=b3182;  

 Linear View (Whole Mode)
View Location
   3315.0  –  3340.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP dacB (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Penicillin Binding Protein 4 (Dacb) From Escherichia Coli  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Penicillin-binding protein 4 precursor  
         PSI-BLAST Penicillin-binding protein 4 precursor
    nr     (database updated : 2007.02.20 )
         BLAST D-alanyl-D-alanine carboxypeptidase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG2027M 
    EcoCyc dacB 

MMBR References
Mol Microbiol. 1991;5(3):675-84.
Penicillin-binding protein 4 of Escherichia coli: molecular cloning of the dacB gene, controlled overexpression, and alterations in murein composition.
Korat B, Mottl H, Keck W. ( 2046551 )
Proc Natl Acad Sci U S A. 1977;74:2976-2979
Mutants of Escherichia coli lacking in highly penicillin-sensitive D-alanine carboxypeptidase activity.
Matsuhashi, M., Y. Takagaki, I. N. Maruyama, S. Tamaki, Y. Nishimura, H. Suzuki, U. Ogino, and Y. Hirota.
J Bacteriol. 1992;174:3261-3269
Deletion of an additional domain located between SXXK and SXN active-site fingerprints in penicillin-binding protein 4 from Escherichia coli.
Mottl, H., P. Nieland, G. De Kort, J. J. Wierenga, and W. Keck.
FEMS Microbiol Lett. 1991;62(2-3):213-20.
Penicillin-binding protein 4 of Escherichia coli shows a novel type of primary structure among penicillin-interacting proteins.
Mottl H, Terpstra P, Keck W. ( 2040429 )
J Bacteriol. 1994;176:7767-7769
Transcriptional control of dacB, which encodes a major sporulation-specific penicillin-binding protein.
Simpson, E. B., T. W. Hancock, and C. E. Buchanan.
Plasmid. 1981;6(1):86-98.
Synthetic ColE1 Plasmids carrying genes for penicillin-binding proteins in Escherichia coli.
Takeda Y, Nishimura A, Nishimura Y, Yamada M, Yasuda S, Suzuki H, Hirota Y. ( 7025060 )

Sequences
Amino acid
FASTA format
0001 MRFSRFIIGL TSCIAFSVQA ANVDEYITQL PAGANLALMV QKVGASAPAI DYHSQQMALP ASTQKVITAL 
0071 AALIQLGPDF RFTTTLETKG NVENGVLKGD LVARFGADPT LKRQDIRNMV ATLKKSGVNQ IDGNVLIDTS 
0141 IFASHDKAPG WPWNDMTQCF SAPPAAAIVD RNCFSVSLYS APKPGDMAFI RVASYYPVTM FSQVRTLPRG 
0211 SAEAQYCELD VVPGDLNRFT LTGCLPQRSE PLPLAFAVQD GASYAGAILK DELKQAGITW SGTLLRQTQV 
0281 NEPGTVVASK QSAPLHDLLK IMLKKSDNMI ADTVFRMIGH ARFNVPGTWR AGSDAVRQIL RQQAGVDIGN 
0351 TIIADGSGLS RHNLIAPATM MQVLQYIAQH DNELNFISML PLAGYDGSLQ YRAGLHQAGV DGKVSAKTGS 
0421 LQGVYNLAGF ITTASGQRMA FVQYLSGYAV EPADQRNRRI PLVRFESRLY KDIYQNN

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             ATAGTCATTT TACCCTGAAG TTCCCGAAGG 
-070 GTCATCGTTT ACTTTATAGG GCGTTGCGCC GTAGTATGAC GGCTCGATTC CAGGTTGTTA GCGCGAGATT 
0001 atgcgatttt ccagatttat catcggattg accagctgta tagcgttcag tgttcaggcc gcaaatgttg 
0071 atgagtacat tactcaactc cccgctggtg ccaaccttgc cctgatggtg caaaaagtcg gcgcgtcggc 
0141 ccccgctatt gattaccaca gtcagcagat ggcgctgcct gccagtaccc agaaagtgat tactgcgctg 
0211 gcggcgttga ttcaactcgg ccccgatttt cgttttacca cgacgcttga aaccaaaggc aatgtggaaa 
0281 acggcgtact taagggtgac ttagtggcgc gatttggtgc cgatccgacg ttaaaacgtc aggatattcg 
0351 caatatggtc gcgactttga aaaaatctgg cgtcaaccaa atcgatggca atgtgttgat agatacctcc 
0421 attttcgcca gccacgataa agcccccggc tggccatgga atgacatgac acaatgcttt agcgctccgc 
0491 ctgccgccgc catagttgac cgcaactgtt tctccgtctc gctctacagt gccccaaagc ctggtgatat 
0561 ggcttttata cgcgtggcat cttattaccc cgttacgatg ttcagccagg tacgcaccct cccccgtggt 
0631 tctgccgaag cgcaatactg cgaactggat gtggtgccag gcgacctgaa ccgctttacg ctgacgggat 
0701 gcctgccaca acgttctgag ccgctcccgt tggcttttgc cgtgcaggat ggagccagct atgccggtgc 
0771 aattctgaaa gatgagttaa aacaggcggg tatcacctgg agcggaacac tgctgcgcca gactcaggtt 
0841 aacgaacctg gaacggtagt tgccagtaaa cagtcggccc cgctgcacga tctgcttaag attatgctga 
0911 aaaagtcgga caacatgatc gccgatacgg ttttccgcat gataggccat gcgcgcttca atgtgcctgg 
0981 aacatggcgg gccgggtcgg acgccgtgcg tcagatcctg cgccagcaag ccggtgtcga tattggaaac 
1051 accattattg ccgatggttc agggctttcg cggcataacc tgattgcccc cgccaccatg atgcaggtgc 
1121 tgcaatacat tgcccaacac gacaatgaac ttaactttat ctccatgctg ccgctggcgg gctatgacgg 
1191 ctctttgcag taccgtgcag gtctgcatca ggcgggcgtg gatggaaaag tctcagcgaa aaccggttcg 
1261 ttgcaggggg tatataacct ggcgggattc attaccacag cgagcgggca acgaatggcg tttgtgcaat 
1331 atctttctgg ctatgcagta gaacctgcgg atcagcgtaa tcgccgtatt ccgttagtgc gttttgaaag 
1401 ccgtttgtat aaagatattt atcagaacaa ttagTCAAAA AGAAACCCCG GCACATGGCC GGGGCTTCAG 
1471 ATTATTGACA AAGTGCGCTT TGTTTATGCC GGATGCGGCG TAAACGCCTT ATCCGGCCTA CAAA