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PEC Original Annotations
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Essentiality
|
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Class
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non-essential
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References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
|
OCL29,30-6 (AK4-11 / BK5-11)
(D)
,
OCL30
(D)
,
OCL30-2
(D)
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Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
deaD
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Related strains
|
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Strains Search
|
Search strains by gene name:
deaD
Search strains by all related name:
deaD b3162 csdA ECK3150 f646 JW5531 mssB rhlD
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General information (Go to Linear View: )
|
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Gene Name
|
deaD
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Alternative name
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b3162,csdA,ECK3150,f646,JW5531,mssB,rhlD
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Location, Length
|
3,303,993 - 3,305,882
(
-
)
;
71.21
min
;
1890
(bp) ,
629
(aa)
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Product
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ATP-dependent RNA helicase
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Operon Name
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yrbN-deaD
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Note
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inducible ATP-independent RNA helicase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009451 - RNA modification
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Function
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enzyme; RNA synthesis, modification, DNA transcription
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Gene Ontology
|
GO:0000166
;
nucleotide binding ( deaD )
GO:0003676
;
nucleic acid binding ( deaD )
GO:0003723
;
RNA binding ( deaD )
GO:0003824
;
catalytic activity ( csdA )
GO:0004386
;
helicase activity ( deaD )
GO:0005515
;
protein binding ( csdA )
GO:0005524
;
ATP binding ( deaD )
GO:0005737
;
cytoplasm ( deaD )
GO:0006350
;
transcription ( deaD )
GO:0006355
;
regulation of transcription, DNA-dependent ( deaD )
GO:0006950
;
response to stress ( deaD )
GO:0008026
;
ATP-dependent helicase activity ( deaD )
GO:0008152
;
metabolic process ( csdA )
GO:0008483
;
transaminase activity ( csdA )
GO:0016787
;
hydrolase activity ( deaD )
GO:0016829
;
lyase activity ( csdA )
GO:0030170
;
pyridoxal phosphate binding ( csdA )
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PID
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87082221
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EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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deaD
(
EG10215
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
DEAD_ECOLI
(
P23304
;
Q8FD90
)
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- Protein name
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Cold-shock DEAD-box protein A
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- Synonyms
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ATP-dependent RNA helicase deaD
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- Gene name
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OrderedLocusNames=b3162, c3916, SF3203, S3420;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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csdA
(
homologous genes of other bacterias
)
deaD
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
|
Chain A, Crystal Structure Of Human Apo-Eif4aiii
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
|
Cold-shock DEAD box protein A
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PSI-BLAST
|
Cold-shock DEAD box protein A
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nr
(database updated :
2007.02.20
)
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BLAST
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inducible ATP-independent RNA helicase [Shigella flexneri 2a str. 2457T]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AMIDATION
RGD
ATP_GTP_A
DEAD_ATP_HELICASE
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MMBR References
|
J Bacteriol. 1998;180:90-95
Role of the cold-box region in the 5' untranslated region of the cspA mRNA in its transient expression at low temperature in Escherichia coli.
Fang, L., Y. Hou, and M. Inouye.
|
Proc Natl Acad Sci U S A. 1996;93:76-80
Cold shock induces a major ribosomal-associated protein that unwinds double-stranded RNA in Escherichia coli.
Jones, P. G., M. Mitta, Y. Kim, W. Jiang, and M. Inouye.
|
J Bacteriol. 1991;173(11):3291-302.
deaD, a new Escherichia coli gene encoding a presumed ATP-dependent RNA helicase, can suppress a mutation in rpsB, the gene encoding ribosomal protein S2.
Toone WM, Rudd KE, Friesen JD.
(
2045359
)
|
Mol Gen Genet. 1994;243(1):9-16.
Multicopy suppressors, mssA and mssB, of an smbA mutation of Escherichia coli.
Yamanaka K, Ogura T, Koonin EV, Niki H, Hiraga S.
(
8190075
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MAEFETTFAD LGLKAPILEA LNDLGYEKPS PIQAECIPHL LNGRDVLGMA QTGSGKTAAF SLPLLQNLDP
0071 ELKAPQILVL APTRELAVQV AEAMTDFSKH MRGVNVVALY GGQRYDVQLR ALRQGPQIVV GTPGRLLDHL
0141 KRGTLDLSKL SGLVLDEADE MLRMGFIEDV ETIMAQIPEG HQTALFSATM PEAIRRITRR FMKEPQEVRI
0211 QSSVTTRPDI SQSYWTVWGM RKNEALVRFL EAEDFDAAII FVRTKNATLE VAEALERNGY NSAALNGDMN
0281 QALREQTLER LKDGRLDILI ATDVAARGLD VERISLVVNY DIPMDSESYV HRIGRTGRAG RAGRALLFVE
0351 NRERRLLRNI ERTMKLTIPE VELPNAELLG KRRLEKFAAK VQQQLESSDL DQYRALLSKI QPTAEGEELD
0421 LETLAAALLK MAQGERTLIV PPDAPMRPKR EFRDRDDRGP RDRNDRGPRG DREDRPRRER RDVGDMQLYR
0491 IEVGRDDGVE VRHIVGAIAN EGDISSRYIG NIKLFASHST IELPKGMPGE VLQHFTRTRI LNKPMNMQLL
0561 GDAQPHTGGE RRGGGRGFGG ERREGGRNFS GERREGGRGD GRRFSGERRE GRAPRRDDST GRRRFGGDA
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ctactttgag ccggttcaca cttttcaatg
-070 aaaattgctg atcaatttca tgatgagtta tgtagactgg ccgccattaa ttttgaggca cacgtactac
0001 atggctgaat tcgaaaccac ttttgcagat ctgggcctga aggctcctat ccttgaagcc cttaacgatc
0071 tgggttacga aaaaccatct ccaattcagg cagagtgtat tccacatctg ctgaatggcc gcgacgttct
0141 gggtatggcc cagacgggga gcggaaaaac tgcagcattc tctttacctc tgttgcagaa tcttgatcct
0211 gagctgaaag caccacagat tctggtgctg gcaccgaccc gcgaactggc ggtacaggtt gctgaagcaa
0281 tgacggattt ctctaaacac atgcgcggcg taaatgtggt tgctctgtac ggcggccagc gttatgacgt
0351 gcaattacgc gccctgcgtc aggggccgca gatcgttgtc ggtactccgg gccgtctgct ggaccacctg
0421 aaacgtggca ctctggacct ctctaaactg agcggtctgg ttctggatga agctgacgaa atgctgcgca
0491 tgggcttcat cgaagacgtt gaaaccatta tggcgcagat cccggaaggt catcagaccg ctctgttctc
0561 tgcaaccatg ccggaagcga ttcgtcgcat tacccgccgc tttatgaaag agccgcagga agtgcgcatt
0631 cagtccagcg tgactacccg tcctgacatc agccagagct actggactgt ctggggtatg cgcaaaaacg
0701 aagcactggt acgtttcctg gaagcggaag attttgatgc ggcgattatc ttcgttcgta ccaaaaacgc
0771 gactctggaa gtggctgaag ctcttgagcg taacggctac aacagcgccg cgctgaacgg tgacatgaac
0841 caggcgctgc gtgaacagac actggaacgc ctgaaagatg gtcgtctgga catcctgatt gcgaccgacg
0911 ttgcagcccg tggcctggac gttgagcgta tcagcctggt agttaactac gatatcccga tggattctga
0981 gtcttacgtt caccgtatcg gtcgtaccgg tcgtgcgggt cgtgctggcc gcgcgctgct gttcgttgag
1051 aaccgcgagc gtcgtctgct gcgcaacatt gaacgtacta tgaagctgac tattccggaa gtagaactgc
1121 cgaacgcaga actgctaggc aaacgccgtc tggaaaaatt cgccgctaaa gtacagcagc agctggaaag
1191 cagcgatctg gatcaatacc gcgcactgct gagcaaaatt cagccgactg ctgaaggtga agagctggat
1261 ctcgaaactc tggctgcggc actgctgaaa atggcacagg gtgaacgtac tctgatcgta ccgccagatg
1331 cgccgatgcg tccgaaacgt gaattccgtg accgtgatga ccgtggtccg cgcgatcgta acgaccgtgg
1401 cccgcgtggt gaccgtgaag atcgtccgcg tcgtgaacgt cgtgatgttg gcgatatgca gctgtaccgc
1471 attgaagtgg gccgcgatga tggtgttgaa gttcgtcata tcgttggtgc gattgctaac gaaggcgaca
1541 tcagcagccg ttacattggt aacatcaagc tgtttgcttc tcactccacc atcgaactgc cgaaaggtat
1611 gccgggtgaa gtgctgcaac actttacgcg cactcgcatt ctcaacaagc cgatgaacat gcagttactg
1681 ggcgatgcac agccgcatac tggcggtgag cgtcgtggcg gtggtcgtgg tttcggtggc gaacgtcgtg
1751 aaggcggtcg taacttcagc ggtgaacgcc gtgaaggtgg ccgtggtgat ggtcgtcgtt ttagcggcga
1821 acgtcgtgaa ggccgcgctc cgcgtcgtga tgattctacc ggtcgtcgtc gtttcggtgg tgatgcgtaa
1891 tcatcgctga acagcgaaca caatctgtaa aataatatat acagccccga tttttaccat cggggctttt
1961 tttctgtctt ttgtactcgt gtactggtac
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