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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Bacteriol. 1989;171(3):1485-91.
Genetic suppression of a dnaG mutation in Escherichia coli.
Katayama T, Murakami Y, Wada C, Ohmori H, Yura T, Nagata T.
(
2646283
)
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Deletion
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OCL34,35-3 (BK2-10 / AK1-10)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
dnaG
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Related strains
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Strains Search
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Search strains by gene name:
dnaG
Search strains by all related name:
dnaG b3066 dnaP ECK3056 JW3038 parB sdgA
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General information (Go to Linear View: )
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Gene Name
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dnaG
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Alternative name
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b3066,dnaP,ECK3056,JW3038,parB,sdgA
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Location, Length
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3,209,129 - 3,210,874
(
+
)
;
69.17
min
;
1746
(bp) ,
581
(aa)
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Product
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DNA primase
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Operon Name
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rpsU-dnaG-rpoD
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Note
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DNA biosynthesis; DNA primase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication
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Function
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enzyme; DNA - replication, repair,restriction/modification
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Gene Ontology
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GO:0003676
;
nucleic acid binding ( dnaG )
GO:0003677
;
DNA binding ( dnaG )
GO:0003896
;
DNA primase activity ( dnaG )
GO:0003899
;
DNA-directed RNA polymerase activity ( dnaG )
GO:0005515
;
protein binding ( dnaG )
GO:0005658
;
alpha DNA polymerase:primase complex ( dnaG )
GO:0006260
;
DNA replication ( dnaG )
GO:0006269
;
DNA replication, synthesis of RNA primer ( dnaG )
GO:0006304
;
DNA modification ( dnaG )
GO:0006350
;
transcription ( dnaG )
GO:0008270
;
zinc ion binding ( dnaG )
GO:0016740
;
transferase activity ( dnaG )
GO:0016779
;
nucleotidyltransferase activity ( dnaG )
GO:0046872
;
metal ion binding ( dnaG )
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PID
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1789447
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EC number
(KEGG Pathway)
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2.7.7.-
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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dnaG
(
EG10239
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
3
more]
)
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Entry name(Acc.no)
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PRIM_ECOLI
(
P02923
;
P02922; Q47613
)
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- Protein name
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DNA primase
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- Synonyms
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EC 2.7.7.-
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- Gene name
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OrderedLocusNames=b3066, z4419, ECs3949, SF3107, S3312;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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dnaG
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Structure Of The Dnag Catalytic Core
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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DNA primase
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PSI-BLAST
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DNA primase
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nr
(database updated :
2007.02.20
)
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BLAST
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COG0358: DNA primase
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
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MMBR References
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Proc Natl Acad Sci U S A. 1979;76:5789-5793
Isolation and characterization of transducing phage coding for a subunit of Escherichia coli RNA polymerase.
Gross, C. A., F. R. Blattner, W. E. Taylor, P. A. Lowe, and R. R. Burgess.
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Mol Gen Genet. 1984;195(3):391-401.
Promotion, termination, and anti-termination in the rpsU-dnaG-rpoD macromolecular synthesis operon of E. coli K-12.
Lupski JR, Ruiz AA, Godson GN.
(
6206376
)
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Mol Gen Genet. 1982;185(1):120-8.
Cloning and characterization of the Escherichia coli chromosomal region surrounding the dnaG Gene, with a correlated physical and genetic map of dnaG generated via transposon Tn5 mutagenesis.
Lupski JR, Smiley BL, Blattner FR, Godson GN.
(
6283308
)
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Mol Gen Genet. 1983;189(1):48-57.
Regulation of the rpsU-dnaG-rpoD macromolecular synthesis operon and the initiation of DNA replication in Escherichia coli K-12.
Lupski JR, Smiley BL, Godson GN.
(
6222240
)
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J Bacteriol. 1985;162(2):830-2.
Novel dnaG mutation in a dnaP mutant of Escherichia coli.
Murakami Y, Nagata T, Schwarz W, Wada C, Yura T.
(
3886637
)
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Mol Gen Genet. 1980;178(3):487-97.
Hybrid plasmid carrying Escherichia coli genes for the primase (dnaG) and RNA polymerase sigma factor (rpoD); gene organization and control of their expression.
Nakamura Y.
(
6993850
)
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J Biol Chem. 1987;262:10475-10480
Structure of Escherichia coli dnaC. Identification of a cysteine residue possibly involved in association with dnaB protein.
Nakayama, N., M. W. Bond, A. Miyajima, J. Kobori, and K.-I. Arai.
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J Bacteriol. 1986;168(2):494-504.
DNA replication termination in Escherichia coli parB (a dnaG allele), parA, and gyrB mutants affected in DNA distribution.
Norris V, Alliotte T, Jaffe A, D'Ari R.
(
3536848
)
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Mol Gen Genet. 1982;187(3):501-9.
Cloning of bacterial DNA replication genes in bacteriophage lambda.
Rowen L, Kobori JA, Scherer S.
(
6294475
)
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Proc Natl Acad Sci U S A. 1982;79:4550-4554
Sequences of the Escherichia coli dnaG primase gene and regulation of its expression.
Smiley, B. L., J. R. Lupski, P. S. Svec, R. MacMacken, and G. N. Godson.
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Cell. 1984;38(2):371-81.
Transcription from a heat-inducible promoter causes heat shock regulation of the sigma subunit of E. coli RNA polymerase.
Taylor WE, Straus DB, Grossman AD, Burton ZF, Gross CA, Burgess RR.
(
6380764
)
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J Biol Chem. 1996;271:21398-21405
The interaction between helicase and primase sets the replication fork clock.
Tougu, K., and K. J. Marians.
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Microbiology 1997;143:585-594
Missense mutations in the 3' end of the Escherichia coli dnaG gene do not abolish primase activity but do confer the chromosome-segregation-defective (par) phenotype.
Versalovic, J., and J. R. Lupski.
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Proc Natl Acad Sci U S A. 1982;79:4907-4911
Regulation of expression of the Escherichia coli dnaG gene and amplification of the dnaG primase.
Wold, M. S., and R. McMacken.
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Cell. 1983;32(2):335-49.
The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12.
Burton ZF, Gross CA, Watanabe KK, Burgess RR.
(
6186393
)
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| Sequences |
Amino acid
FASTA format
|
0001 MAGRIPRVFI NDLLARTDIV DLIDARVKLK KQGKNFHACC PFHNEKTPSF TVNGEKQFYH CFGCGAHGNA
0071 IDFLMNYDKL EFVETVEELA AMHNLEVPFE AGSGPSQIER HQRQTLYQLM DGLNTFYQQS LQQPVATSAR
0141 QYLEKRGLSH EVIARFAIGF APPGWDNVLK RFGGNPENRQ SLIDAGMLVT NDQGRSYDRF RERVMFPIRD
0211 KRGRVIGFGG RVLGNDTPKY LNSPETDIFH KGRQLYGLYE AQQDNAEPNR LLVVEGYMDV VALAQYGINY
0281 AVASLGTSTT ADHIQLLFRA TNNVICCYDG DRAGRDAAWR ALETALPYMT DGRQLRFMFL PDGEDPDTLV
0351 RKEGKEAFEA RMEQAMPLSA FLFNSLMPQV DLSTPDGRAR LSTLALPLIS QVPGETLRIY LRQELGNKLG
0421 ILDDSQLERL MPKAAESGVS RPVPQLKRTT MRILIGLLVQ NPELATLVPP LENLDENKLP GLGLFRELVN
0491 TCLSQPGLTT GQLLEHYRGT NNAATLEKLS MWDDIADKNI AEQTFTDSLN HMFDSLLELR QEELIARERT
0561 HGLSNEERLE LWTLNQELAK K
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 GCGTTGCTCT CCGATCAGAC CGAGTTGTAG
-070 TTGTAAGGCC GTGCTTCCGA AAGGAATGCG CGGCTTATTT TCGTTTATGA ATTGCTAAAA ATCGGGGCCT
0001 atggctggac gaatcccacg cgtattcatt aatgatctgc tggcacgcac tgacatcgtc gatctgatcg
0071 atgcccgtgt gaagctgaaa aagcagggca agaatttcca cgcgtgttgt ccattccaca acgagaaaac
0141 cccgtccttc accgttaacg gtgagaaaca gttttaccac tgctttggat gtggcgcgca cggcaacgcg
0211 atcgacttcc tgatgaacta cgacaagctc gagttcgtcg aaacggtcga agagctggca gcaatgcaca
0281 atcttgaagt gccatttgaa gcaggcagcg gccccagcca gatcgagcgc catcagaggc aaacgcttta
0351 tcagttgatg gacggtctga atacgtttta ccaacaatct ttacaacaac ctgttgccac gtctgcgcgc
0421 cagtatctgg aaaaacgcgg attaagccac gaggttatcg ctcgctttgc gattggtttt gcgccccccg
0491 gctgggacaa cgtcctgaag cggtttggcg gcaatccaga aaatcgccag tcattgattg atgcggggat
0561 gttggtcact aacgatcagg gacgcagtta cgatcgtttc cgcgagcggg tgatgttccc cattcgcgat
0631 aaacgcggtc gggtgattgg ttttggcggg cgcgtgctgg gcaacgatac ccccaaatac ctgaactcgc
0701 cggaaacaga cattttccat aaaggccgcc agctttacgg tctttatgaa gcgcagcagg ataacgctga
0771 acccaatcgt ctgcttgtgg tcgaaggcta tatggacgtg gtggcgctgg cgcaatacgg cattaattac
0841 gccgttgcgt cgttaggtac gtcaaccacc gccgatcaca tacaactgtt gttccgcgcg accaacaatg
0911 tcatttgctg ttatgacggc gaccgtgcag gccgcgatgc cgcctggcga gcgctggaaa cggcgctgcc
0981 ttacatgaca gacggccgtc agctacgctt tatgtttttg cctgatggcg aagaccctga cacgctagta
1051 cgaaaagaag gtaaagaagc gtttgaagcg cggatggagc aggcgatgcc actctccgca tttctgttta
1121 acagtctgat gccgcaagtt gatctgagta cccctgacgg gcgcgcacgt ttgagtacgc tggcactacc
1191 attgatatcg caagtgccgg gcgaaacgct gcgaatatat cttcgtcagg aattaggcaa caaattaggc
1261 atacttgatg acagccagct tgaacgatta atgccaaaag cggcagagag cggcgtttct cgccctgttc
1331 cgcagctaaa acgcacgacc atgcgtatac ttatagggtt gctggtgcaa aatccagaat tagcgacgtt
1401 ggtcccgccg cttgagaatc tggatgaaaa taagctccct ggacttggct tattcagaga actggtcaac
1471 acttgtctct cccagccagg tctgaccacc gggcaacttt tagagcacta tcgtggtaca aataatgctg
1541 ccacccttga aaaactgtcg atgtgggacg atatagcaga taagaatatt gctgagcaaa ccttcaccga
1611 ctcactcaac catatgtttg attcgctgct tgaactgcgc caggaagagt taatcgctcg tgagcgcacg
1681 catggtttaa gcaacgaaga acgcctggag ctctggacat taaaccagga gctggcgaaa aagtgaTTTA
1751 ACGGCTTAAG TGCCGAAGAG CGATCGGGAA GCCCCCGACA GCCGCACTGA GAGGCAGCGG CAAATATATA
1821 AGTACGCCCT CGTAATTATC GTTGGC
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