MG1655
W3110
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Gene Report : thrB
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
FEMS Microbiol Lett. 1996;135(2-3):275-80.
4-Phospho-hydroxy-L-threonine is an obligatory intermediate in pyridoxal 5'-phosphate coenzyme biosynthesis in Escherichia coli K-12.
Zhao G, Winkler ME. ( 8595869 )
     Deletion OCR27 (D)  ,  OCR27-1 (D)  ,  OCR27-6 (D)  ,  OCR27-7 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  thrB
Related strains
     Strains Search Search strains by gene name:  thrB   Search strains by all related name:  thrB b0003 ECK0003 JW0002

General information  (Go to Linear View:)
 Gene Name thrB  
 Alternative name b0003,ECK0003,JW0002  
 Location, Length 2,801 - 3,733 (  +  ) ;   0.06 min ; 933 (bp) ,   310 (aa) Go to Linear View
 Product homoserine kinase  
 Operon Name thrLABC  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009088 - threonine biosynthetic process  
 Function enzyme; Amino acid biosynthesis: Threonine,1.5.1.8 metabolism; building block biosynthesis; amino acids; threonine,7.1 location of gene products; cytoplasm  
 Gene Ontology GO:0000166; nucleotide binding ( thrB )
GO:0004413; homoserine kinase activity ( thrB )
GO:0005524; ATP binding ( thrB )
GO:0005737; cytoplasm ( thrB )
GO:0006566; threonine metabolic process ( thrB )
GO:0008652; amino acid biosynthetic process ( thrB )
GO:0009088; threonine biosynthetic process ( thrB )
GO:0016301; kinase activity ( thrB )
GO:0016310; phosphorylation ( thrB )
GO:0016740; transferase activity ( thrB )
 PID 1786184  
 EC number
  (KEGG Pathway)
 
2.7.1.39  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) KHSE_ECOLI ( P00547 )
    -  Protein name Homoserine kinase  
    -  Synonyms EC 2.7.1.39, HK  
    -  Gene name Name=thrB; OrderedLocusNames=b0003;  

 Linear View (Whole Mode)
View Location
   4625.0  –  25.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP thrB (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Galactokinase From Pyrococcus Horikoshi  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Homoserine kinase  
         PSI-BLAST Homoserine kinase
    nr     (database updated : 2007.02.20 )
         BLAST homoserine kinase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    CK2_PHOSPHO_SITE    MYRISTYL    PROKAR_LIPOPROTEIN    GHMP_KINASES_ATP     

Other Cross-Reference
    COG COG0083E 
    EcoCyc thrB 

MMBR References
J Bacteriol. 1979;140(1):106-13.
Structural and functional analysis of cloned deoxyribonucleic acid containing the trpR-thr region of the Escherichia coli chromosome.
Gunsalus RP, Zurawski G, Yanofsky C. ( 159284 )
J Bacteriol. 1985;161(1):461-2.
Evidence for an internal promoter in the Escherichia coli threonine operon.
Saint Girons I, Margarita D. ( 3918011 )
FEMS Microbiol Lett. 1996;135(2-3):275-80.
4-Phospho-hydroxy-L-threonine is an obligatory intermediate in pyridoxal 5'-phosphate coenzyme biosynthesis in Escherichia coli K-12.
Zhao G, Winkler ME. ( 8595869 )
Nucleic Acids Res. 1981;9:339-347
Nucleotide sequence of the thrB gene of E. coli, and its two adjacent regions; the thrAB and thrBC junctions.
Cossart, P., M. Katinka, and M. Yaniv.
Mol Gen Genet. 1979;175(1):39-44.
Construction and expression of a hybrid plasmid containing the Escherichia coli thrA and thrB genes.
Cossart P, Katinka M, Yaniv M, Saint Girons I, Cohen GN. ( 390305 )

Sequences
Amino acid
FASTA format
0001 MVKVYAPASS ANMSVGFDVL GAAVTPVDGA LLGDVVTVEA AETFSLNNLG RFADKLPSEP RENIVYQCWE 
0071 RFCQELGKQI PVAMTLEKNM PIGSGLGSSA CSVVAALMAM NEHCGKPLND TRLLALMGEL EGRISGSIHY 
0141 DNVAPCFLGG MQLMIEENDI ISQQVPGFDE WLWVLAYPGI KVSTAEARAI LPAQYRRQDC IAHGRHLAGF 
0211 IHACYSRQPE LAAKLMKDVI AEPYRERLLP GFRQARQAVA EIGAVASGIS GSGPTLFALC DKPETAQRVA 
0281 DWLGKNYLQN QEGFVHICRL DTAGARVLEN 

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             CCGTTGGTAC TGCGCGGATA TGGTGCGGGC 
-070 AATGACGTTA CAGCTGCCGG TGTCTTTGCT GATCTGCTAC GTACCCTCTC ATGGAAGTTA GGAGTCTGAC 
0001 atggttaaag tttatgcccc ggcttccagt gccaatatga gcgtcgggtt tgatgtgctc ggggcggcgg 
0071 tgacacctgt tgatggtgca ttgctcggag atgtagtcac ggttgaggcg gcagagacat tcagtctcaa 
0141 caacctcgga cgctttgccg ataagctgcc gtcagaacca cgggaaaata tcgtttatca gtgctgggag 
0211 cgtttttgcc aggaactggg taagcaaatt ccagtggcga tgaccctgga aaagaatatg ccgatcggtt 
0281 cgggcttagg ctccagtgcc tgttcggtgg tcgcggcgct gatggcgatg aatgaacact gcggcaagcc 
0351 gcttaatgac actcgtttgc tggctttgat gggcgagctg gaaggccgta tctccggcag cattcattac 
0421 gacaacgtgg caccgtgttt tctcggtggt atgcagttga tgatcgaaga aaacgacatc atcagccagc 
0491 aagtgccagg gtttgatgag tggctgtggg tgctggcgta tccggggatt aaagtctcga cggcagaagc 
0561 cagggctatt ttaccggcgc agtatcgccg ccaggattgc attgcgcacg ggcgacatct ggcaggcttc 
0631 attcacgcct gctattcccg tcagcctgag cttgccgcga agctgatgaa agatgttatc gctgaaccct 
0701 accgtgaacg gttactgcca ggcttccggc aggcgcggca ggcggtcgcg gaaatcggcg cggtagcgag 
0771 cggtatctcc ggctccggcc cgaccttgtt cgctctgtgt gacaagccgg aaaccgccca gcgcgttgcc 
0841 gactggttgg gtaagaacta cctgcaaaat caggaaggtt ttgttcatat ttgccggctg gatacggcgg 
0911 gcgcacgagt actggaaaac taaATGAAAC TCTACAATCT GAAAGATCAC AACGAGCAGG TCAGCTTTGC 
0981 GCAAGCCGTA ACCCAGGGGT TGGGCAAAAA TCAGGGGCTG TTTTTTCCGC ACG