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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
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OCL38(-Km)
(LD)
,
OCL38
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
mutY
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Related strains
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Strains Search
|
Search strains by gene name:
mutY
Search strains by all related name:
mutY b2961 ECK2956 JW2928 micA mutB
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General information (Go to Linear View: )
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Gene Name
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mutY
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Alternative name
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b2961,ECK2956,JW2928,micA,mutB
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Location, Length
|
3,101,035 - 3,102,087
(
+
)
;
66.84
min
;
1053
(bp) ,
350
(aa)
|
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Product
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adenine DNA glycosylase
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Operon Name
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mutY-yggX-mltC-nupG
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Note
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adenine glycosylase; G.C --> T.A transversions; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006281 - DNA repair
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Function
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enzyme; DNA - replication, repair,restriction/modification
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Gene Ontology
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GO:0003677
;
DNA binding ( mutY )
GO:0003824
;
catalytic activity ( mutY )
GO:0004519
;
endonuclease activity ( mutY )
GO:0005506
;
iron ion binding ( mutY )
GO:0005622
;
intracellular ( mutY )
GO:0006281
;
DNA repair ( mutY )
GO:0006284
;
base-excision repair ( mutY )
GO:0006974
;
response to DNA damage stimulus ( mutY )
GO:0008152
;
metabolic process ( mutY )
GO:0016787
;
hydrolase activity ( mutY )
GO:0016798
;
hydrolase activity, acting on glycosyl bonds ( mutY )
GO:0019104
;
DNA N-glycosylase activity ( mutY )
GO:0043565
;
sequence-specific DNA binding ( mutY )
GO:0046872
;
metal ion binding ( mutY )
GO:0051536
;
iron-sulfur cluster binding ( mutY )
GO:0051539
;
4 iron, 4 sulfur cluster binding ( mutY )
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PID
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1789331
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EC number
(KEGG Pathway)
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3.2.2.-
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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MUTY_ECOLI
(
P17802
)
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- Protein name
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A/G-specific adenine glycosylase
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- Synonyms
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EC 3.2.2.-
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- Gene name
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Name=mutY; Synonyms=micA; OrderedLocusNames=b2961;
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MMBR References
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J Bacteriol. 1968;96(4):975-80.
Selection of a mutant of Escherichia coli which has high mutation rates.
Helling RB.
(
4879569
)
|
Nucleic Acids Res. 1990;18:3841-3845
MutY, an adenine glycosylase active on G-A mispairs, has homology to endonuclease III.
Michaels, M. L., L. Pham, Y. Nghiem, C. Cruz, and J. H. Miller.
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Proc Natl Acad Sci U S A. 1988;85:2709-2713
The mutY gene: a mutator locus in Escherichia coli that generates G . C-->T . A transversions.
Nghiem, Y., M. Cabrera, C. G. Cupples, and J. H. Miller.
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J Bacteriol. 1993;175:7732-7736
Patch length of localized repair events: role of DNA polymerase I in mutY-dependent mismatch repair.
Radicella, J. P., E. A. Clark, S. Chen, and M. S. Fox.
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J Bacteriol. 1967;94(1):38-47.
Mutator gene of Escherichia coli B.
Siegel EC, Bryson V.
(
5338974
)
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Proc Natl Acad Sci U S A. 1992;89:8779-8783
Escherichia coli MutY protein has both N-glycosylase and apurinic/apyrimidinic endonuclease activities on A . C and A . G mispairs.
Tsai-Wu, J. J., H. F. Liu, and A. L. Lu.
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J Bacteriol. 1991;173(6):1902-10.
Nucleotide sequence of the Escherichia coli micA gene required for A/G-specific mismatch repair: identity of micA and mutY.
Tsai-Wu JJ, Radicella JP, Lu AL.
(
2001994
)
|
Genetics 1974;77:169-184
Selection for high mutation rates in chemostats.
Cox, E. C., and T. Gibson.
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| Sequences |
Amino acid
FASTA format
|
0001 MQASQFSAQV LDWYDKYGRK TLPWQIDKTP YKVWLSEVML QQTQVATVIP YFERFMARFP TVTDLANAPL
0071 DEVLHLWTGL GYYARARNLH KAAQQVATLH GGKFPETFEE VAALPGVGRS TAGAILSLSL GKHFPILDGN
0141 VKRVLARCYA VSGWPGKKEV ENKLWSLSEQ VTPAVGVERF NQAMMDLGAM ICTRSKPKCS LCPLQNGCIA
0211 AANNSWALYP GKKPKQTLPE RTGYFLLLQH EDEVLLAQRP PSGLWGGLYC FPQFADEESL RQWLAQRQIA
0281 ADNLTQLTAF RHTFSHFHLD IVPMWLPVSS FTGCMDEGNA LWYNLAQPPS VGLAAPVERL LQQLRTGAPV
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GATGCAAGCA TGATAAGGCC GTGGCTGCGG
-070 AAAGTTCCGG TTTACACCCT GCCGTCGCTG TGCTGCAATC TTGCCCCCAA CAACAGTGAA TTCGGTGACC
0001 atgcaagcgt cgcaattttc agcccaggtt ctggactggt acgataaata cgggcgaaaa actctgccct
0071 ggcaaattga caagacgccc tacaaagtat ggctctcaga agtgatgttg caacaaactc aggttgcgac
0141 cgttatcccc tattttgaac gctttatggc gcgcttcccg acggtgaccg atctcgccaa tgcgccgctc
0211 gacgaagttc tccacttgtg gaccgggctt ggctattacg cccgcgcgcg caatctgcat aaagcggcac
0281 aacaagtggc gaccttacac ggcggtaaat tcccggaaac ctttgaggaa gttgcagcac tgccgggcgt
0351 cgggcgttcc accgcaggcg cgattctctc gctttctctg ggtaagcact ttccgattct cgacggtaac
0421 gtcaaacgcg tgctggcgcg ctgctatgct gtaagcggct ggcctgggaa aaaagaggtc gagaataaat
0491 tatggagttt gagcgagcag gtgacgcccg cggttggcgt ggaacggttt aatcaggcga tgatggattt
0561 gggtgcgatg atttgtacgc gctcgaaacc gaaatgttcg ctctgtccgc tacaaaacgg atgtattgcc
0631 gccgccaaca atagctgggc gctttatccg ggcaaaaaac cgaaacagac gctgccggag cgcaccggct
0701 actttttgct attacagcac gaagatgaag tattgctggc gcagcgtccg ccgagcggat tgtggggcgg
0771 tttatactgt ttcccgcagt ttgccgacga agaaagtttg cggcagtggc tggcgcaacg gcagattgct
0841 gccgataacc tgacgcaact gaccgcgttt cggcatacct tcagccattt ccacttagat attgtgccta
0911 tgtggcttcc cgtgtcgtca ttcaccggct gcatggatga aggcaatgcg ctctggtata acttagcgca
0981 accgccgtca gttggcctag cggctcccgt ggagcgtttg ttacagcagt tacgcactgg cgcgccggtt
1051 tagCGCGTGA GTCGATAAAG AGGATGATTT ATGAGCAGAA CGATTTTTTG TACTTTCCTG CAACGTGAAG
1121 CAGAAGGTCA GGATTTTCAG CTGTACCCCG GCG
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