|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
Mol Microbiol. 2002;43(6):1651-6.
Studies on the role of the metK gene product of Escherichia coli K-12.
Wei Y, Newman EB.
(
11952912
)
|
|
|
Deletion
|
OCL39
(D)
,
OCL39-10-2
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
metK
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
metK
Search strains by all related name:
metK b2942 ECK2937 JW2909 metX
|
General information (Go to Linear View: )
|
|
Gene Name
|
metK
|
|
Alternative name
|
b2942,ECK2937,JW2909,metX
|
|
Location, Length
|
3,084,728 - 3,085,882
(
+
)
;
66.49
min
;
1155
(bp) ,
384
(aa)
|
|
Product
|
S-adenosylmethionine synthetase
|
|
Operon Name
|
metK
|
|
Note
|
methionine adenosyltransferase 1 (AdoMet synthetase); methyl and propylamine donor, corepressor of met genes; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006556 - S-adenosylmethionine biosynthetic process
|
|
Function
|
enzyme; Central intermediary metabolism: Pool, multipurpose conversions
|
|
Gene Ontology
|
GO:0000166
;
nucleotide binding ( metK )
GO:0000287
;
magnesium ion binding ( metK )
GO:0004478
;
methionine adenosyltransferase activity ( metK )
GO:0005524
;
ATP binding ( metK )
GO:0005737
;
cytoplasm ( metK )
GO:0006730
;
one-carbon compound metabolic process ( metK )
GO:0016740
;
transferase activity ( metK )
GO:0030955
;
potassium ion binding ( metK )
GO:0046872
;
metal ion binding ( metK )
GO:0050897
;
cobalt ion binding ( metK )
|
|
PID
|
1789311
|
EC number
(KEGG Pathway)
|
2.5.1.6
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
metK
(
EG10589
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show details
)
|
Entry name(Acc.no)
|
METK_ECOLI
(
P04384
;
P30869
)
|
|
- Protein name
|
S-adenosylmethionine synthetase
|
|
- Synonyms
|
EC 2.5.1.6, Methionine adenosyltransferase, AdoMet synthetase, MAT
|
|
- Gene name
|
OrderedLocusNames=b2942, c3528, z4287, ECs3818, SF2933, S3137;
|
|
MMBR References
|
J Bacteriol. 1973;115(1):57-67.
Properties of metK mutants of Escherichia coli K-12.
Greene RC, Hunter JS, Coch EH.
(
4577753
)
|
J Bacteriol. 1977;132(3):832-40.
Isolation of a metK mutant with a temperature-sensitive S-adenosylmethionine synthetase.
Hafner EW, Tabor CW, Tabor H.
(
336609
)
|
Genet Res. 1979;33(1):49-55.
Glutamyl-gamma-methyl ester acts as a methionine analogue in Escherichia coli: analogue resistant mutants map at the metJ and metK loci.
Kraus J, Soll D, Low KB.
(
383574
)
|
J Biol Chem. 1984;259:14505-14507
The sequence of metK, the structural gene for S-adenosylmethionine synthetase in Escherichia coli.
Markham, G. D., J. DeParasis, and J. Gatmaitan.
|
J Bacteriol. 1990;172(8):4489-96.
Novel Escherichia coli K-12 mutants impaired in S-adenosylmethionine synthesis.
Satishchandran C, Taylor JC, Markham GD.
(
2115868
)
|
Gene. 1984;30(1-3):129-36.
Expression of the cloned genes encoding the putrescine biosynthetic enzymes and methionine adenosyltransferase of Escherichia coli (speA, speB, speC and metK).
Boyle SM, Markham GD, Hafner EW, Wright JM, Tabor H, Tabor CW.
(
6392022
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MAKHLFTSES VSEGHPDKIA DQISDAVLDA ILEQDPKARV ACETYVKTGM VLVGGEITTS AWVDIEEITR
0071 NTVREIGYVH SDMGFDANSC AVLSAIGKQS PDINQGVDRA DPLEQGAGDQ GLMFGYATNE TDVLMPAPIT
0141 YAHRLVQRQA EVRKNGTLPW LRPDAKSQVT FQYDDGKIVG IDAVVLSTQH SEEIDQKSLQ EAVMEEIIKP
0211 ILPAEWLTSA TKFFINPTGR FVIGGPMGDC GLTGRKIIVD TYGGMARHGG GAFSGKDPSK VDRSAAYAAR
0281 YVAKNIVAAG LADRCEIQVS YAIGVAEPTS IMVETFGTEK VPSEQLTLLV REFFDLRPYG LIQMLDLLHP
0351 IYKETAAYGH FGREHFPWEK TDKAQLLRDA AGLK
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GCCAGTGTTT TTAACCTGCA GAGTCGTGGT
-070 AGGATCCGCT ACCACAGAAA ATCCACACAA CAGTTTGAGC TAACCAAATT CTCTTTAGGT GATATTAAAT
0001 atggcaaaac acctttttac gtccgagtcc gtctctgaag ggcatcctga caaaattgct gaccaaattt
0071 ctgatgccgt tttagacgcg atcctcgaac aggatccgaa agcacgcgtt gcttgcgaaa cctacgtaaa
0141 aaccggcatg gttttagttg gcggcgaaat caccaccagc gcctgggtag acatcgaaga gatcacccgt
0211 aacaccgttc gcgaaattgg ctatgtgcat tccgacatgg gctttgacgc taactcctgt gcggttctga
0281 gcgctatcgg caaacagtct cctgacatca accagggcgt tgaccgtgcc gatccgctgg aacagggcgc
0351 gggtgaccag ggtctgatgt ttggctacgc aactaatgaa accgacgtgc tgatgccagc acctatcacc
0421 tatgcacacc gtctggtaca gcgtcaggct gaagtgcgta aaaacggcac tctgccgtgg ctgcgcccgg
0491 acgcgaaaag ccaggtgact tttcagtatg acgacggcaa aatcgttggt atcgatgctg tcgtgctttc
0561 cactcagcac tctgaagaga tcgaccagaa atcgctgcaa gaagcggtaa tggaagagat catcaagcca
0631 attctgcccg ctgaatggct gacttctgcc accaaattct tcatcaaccc gaccggtcgt ttcgttatcg
0701 gtggcccaat gggtgactgc ggtctgactg gtcgtaaaat tatcgttgat acctacggcg gcatggcgcg
0771 tcacggtggc ggtgcattct ctggtaaaga tccatcaaaa gtggaccgtt ccgcagccta cgcagcacgt
0841 tatgtcgcga aaaacatcgt tgctgctggc ctggccgatc gttgtgaaat tcaggtttcc tacgcaatcg
0911 gcgtggctga accgacctcc atcatggtag aaactttcgg tactgagaaa gtgccttctg aacaactgac
0981 cctgctggta cgtgagttct tcgacctgcg cccatacggt ctgattcaga tgctggatct gctgcacccg
1051 atctacaaag aaaccgcagc atacggtcac tttggtcgtg aacatttccc gtgggaaaaa accgacaaag
1121 cgcagctgct gcgcgatgct gccggtctga agtaaTCTTT CTTCACCTGC GTTCAAAGGC CAGCCTCGCG
1191 CTGGCCTTTT TCTTTTGGAT AGGCGTTCAC GCCGCATCCG GCAAAAAAAC CGCCCGCACA ATAAC
|
