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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
|
OCL40
(D)
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Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
argP
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Related strains
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Strains Search
|
Search strains by gene name:
argP
Search strains by all related name:
argP b2916 can canR ECK2912 iciA JW2883
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General information (Go to Linear View: )
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Gene Name
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argP
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Alternative name
|
b2916,can,canR,ECK2912,iciA,JW2883
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Location, Length
|
3,057,775 - 3,058,668
(
+
)
;
65.90
min
;
894
(bp) ,
297
(aa)
|
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Product
|
DNA-binding transcriptional activator, replication initiation inhibitor
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Operon Name
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argP
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Note
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replication initiation inhibitor, binds to 13-mers at oriC; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0016564 - transcription repressor activity; GO_process: GO:0006261 - DNA-dependent DNA replication; GO_process: GO:0006350 - transcription
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Function
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regulator; DNA - replication, repair,restriction/modification
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Gene Ontology
|
GO:0003677
;
DNA binding ( iciA )
GO:0003700
;
transcription factor activity ( iciA )
GO:0004089
;
carbonate dehydratase activity ( can )
GO:0005515
;
protein binding ( can )
GO:0006350
;
transcription ( iciA )
GO:0006355
;
regulation of transcription, DNA-dependent ( iciA )
GO:0008156
;
negative regulation of DNA replication ( iciA )
GO:0008270
;
zinc ion binding ( can )
GO:0015976
;
carbon utilization ( can )
GO:0016829
;
lyase activity ( can )
GO:0046872
;
metal ion binding ( can )
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PID
|
1789283
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EC number
(KEGG Pathway)
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SWISS-PROT
(
Show details
)
|
Entry name(Acc.no)
|
ICIA_ECOLI
(
P24194
)
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- Protein name
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Chromosome initiation inhibitor
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- Synonyms
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OriC replication inhibitor
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- Gene name
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OrderedLocusNames=b2916, c3497, z4253, ECs3786, SF2901, S3101;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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can
(
homologous genes of other bacterias
)
argP
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Dntr From Burkholderia Sp. Strain Dnt
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Chromosome initiation inhibitor (OriC replication inhibitor)
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PSI-BLAST
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Chromosome initiation inhibitor
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nr
(database updated :
2007.02.20
)
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BLAST
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chromosome replication initiation inhibitor protein [Escherichia coli CFT073]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
MYRISTYL
HTH_LYSR_FAMILY
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MMBR References
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Biochem Genet. 1975;13(1-2):109-24.
Overproduction of lysine by mutant strains of Escherichia coli with defective lysine transport systems.
Halsall DM.
(
1095014
)
|
Cell. 1990;63(2):325-31.
A novel protein binds a key origin sequence to block replication of an E. coli minichromosome.
Hwang DS, Kornberg A.
(
2208289
)
|
J Biol Chem. 1992;267:23087-23091
Opposed actions of regulatory proteins, DnaA and IciA, in opening the replication origin of Escherichia coli.
Hwang, D. S., and A. Kornberg.
|
J Biol Chem. 1992;267:2209-2213
IciA protein, a specific inhibitor of initiation of Escherichia coli chromosomal replication.
Hwang, D. S., B. Thony, and A. Kornberg.
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Mol Microbiol. 1996;19(2):389-96.
Transcriptional activation of the dnaA gene encoding the initiator for oriC replication by IciA protein, an inhibitor of in vitro oriC replication in Escherichia coli.
Lee YS, Kim H, Hwang DS.
(
8825783
)
|
Fed Proc. 1965;24(5):1239-42.
Genetic defects affecting an arginine permease and repression of arginine synthesis in Escherichia coli.
Maas WK.
(
5320107
)
|
Mol Gen Genet. 1972;118(3):235-45.
Mutations affecting uridine monophosphate pyrophosphorylase or the argR gene in Escherichia coli. Effects on carbamoyl phosphate and pyrimidine biosynthesis and on uracil uptake.
Pierard A, Glansdorff N, Yashphe J.
(
4343250
)
|
J Bacteriol. 1973;116(2):627-35.
Basic amino acid transport in Escherichia coli: properties of canavanine-resistant mutants.
Rosen BP.
(
4583244
)
|
Proc Natl Acad Sci U S A. 1991;88:4066-4070
iciA, an Escherichia coli gene encoding a specific inhibitor of chromosomal initiation of replication in vitro.
Thony, B., D. S. Hwang, L. Fradkin, and A. Kornberg.
|
Submitted for publication.
Celis, R. T. F.
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| Sequences |
Amino acid
FASTA format
|
0001 MKRPDYRTLQ ALDAVIRERG FERAAQKLCI TQSAVSQRIK QLENMFGQPL LVRTVPPRPT EQGQKLLALL
0071 RQVELLEEEW LGDEQTGSTP LLLSLAVNAD SLATWLLPAL APVLADSPIR LNLQVEDETR TQERLRRGEV
0141 VGAVSIQHQA LPSCLVDKLG ALDYLFVSSK PFAEKYFPNG VTRSALLKAP VVAFDHLDDM HQAFLQQNFD
0211 LPPGSVPCHI VNSSEAFVQL ARQGTTCCMI PHLQIEKELA SGELIDLTPG LFQRRMLYWH RFAPESRMMR
0281 KVTDALLDYG HKVLRQD
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GGATACAAAT GTTACCCGCC GACGGCTTCG
-070 GTATATGCAA CCTGACACAA AATTGTGTCA TAGTGCAGGA AAAAGCATTT ACCAGGAGCA GACAACAGCA
0001 atgaaacgcc cggactacag aacattacag gcactggatg cggtgatacg tgaacgagga tttgagcgcg
0071 cggcacaaaa gctgtgcatt acacaatcag ccgtctcaca gcgcattaag caactggaaa atatgttcgg
0141 gcagccgctg ttggtgcgta ccgtaccgcc gcgcccgacg gaacaagggc aaaaactgct ggcactgctg
0211 cgccaggtgg agttgctgga agaagagtgg ctgggcgatg aacaaaccgg ttcgactccg ctgctgcttt
0281 cactggcggt caacgccgac agtctggcga cgtggttgct tcctgcactg gctcctgtgt tggctgattc
0351 gcctatccgc ctcaacttgc aggtagaaga tgaaacccgc actcaggaac gtctgcgccg cggcgaagtg
0421 gtcggcgcgg tgagtattca acatcaggcg ctgccgagtt gtcttgtcga taaacttggt gcgctcgact
0491 atctgttcgt cagctcaaaa ccctttgccg aaaaatattt ccctaacggc gtaacgcgtt cggcattact
0561 gaaagcgcca gtggtcgcgt ttgaccatct tgacgatatg caccaggcct ttttgcagca aaacttcgat
0631 ctgcctccag gcagcgtgcc ctgccatatc gttaattctt cagaagcgtt cgtacaactt gctcgccagg
0701 gcaccacctg ctgtatgatc ccgcacctgc aaatcgagaa agagctggcc agcggtgaac tgattgactt
0771 aacgcctggg ctatttcaac gacggatgct ctactggcac cgctttgctc ctgaaagccg catgatgcgt
0841 aaagtcactg atgcgttact cgattatggt cacaaagtcc ttcgtcagga ttaaTCCATC AAATAATGCC
0911 TGATAGCACA TATCAGGCGT TGTCCTCACT TCTTTTTGTA TTCCTTGAAT CACATCACAA AATAGACAAA
0981 TCTCAGGCGG CAAA
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