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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Bacteriol. 1988;170(11):5378-81.
Conditionally lethal and recessive UGA-suppressor mutations in the prfB gene encoding peptide chain release factor 2 of Escherichia coli.
Kawakami K, Inada T, Nakamura Y.
(
3053663
)
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Deletion
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OCL41,42-1
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
prfB
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Related strains
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Strains Search
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Search strains by gene name:
prfB
Search strains by all related name:
prfB b2891 ECK2886 JW5847 supK
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General information (Go to Linear View: )
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Gene Name
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prfB
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Alternative name
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b2891,ECK2886,JW5847,supK
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Location, Length
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3,033,206 - 3,034,304
(
-
)
;
65.38
min
;
1099
(bp) ,
365
(aa)
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Product
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peptide chain release factor RF-2
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Operon Name
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xerD-dsbC-recJ-prfB-lysS
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Note
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an in-frame premature UGA termination codon is located within the prfB sequence, and a naturally occuring +1 frameshift is required for synthesis of RF-2; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006412 - translation
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Function
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factor; Proteins - translation and modification,2.3.2 information transfer; protein related; translation,7.1 location of gene products; cytoplasm
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Gene Ontology
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GO:0003747
;
translation release factor activity ( prfB )
GO:0005737
;
cytoplasm ( prfB )
GO:0006412
;
translation ( prfB )
GO:0006415
;
translational termination ( prfB )
GO:0016149
;
translation release factor activity, codon specific ( prfB )
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PID
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2367172
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EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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prfB
(
EG10762
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
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RF2_ECOLI
(
P07012
;
P76642
)
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- Protein name
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Peptide chain release factor 2
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- Synonyms
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RF-2
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- Gene name
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Name=prfB; Synonyms=supK; OrderedLocusNames=b2891;
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MMBR References
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J Biol Chem. 1989;264:20054-20059
Molecular cloning and expression of ribosome releasing factor.
Ichikawa, S., and A. Kaji.
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Proc Natl Acad Sci U S A. 1988;85:5620-5624
Chromosomal location and structure of the operon encoding peptide-chain-release factor 2 of Escherichia coli.
Kawakami, K., Y. H. Jonsson, G. R. Bjork, H. Ikeda, and H. Nakamura.
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J Bacteriol. 1995;177:5547-5553
Comparative characterization of release factor RF-3 genes of Escherichia coli, Salmonella typhimurium, and Dichelobacter nodosus.
Kawazu, Y., K. Ito, K. Matsumura, and Y. Nakamura.
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J Bacteriol. 1988;170(10):4537-41.
Rapid and precise mapping of the Escherichia coli release factor genes by two physical approaches.
Lee CC, Kohara Y, Akiyama K, Smith CL, Craigen WJ, Caskey CT.
(
3049538
)
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| Sequences |
Amino acid
FASTA format
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0001 MFEINPVNNR IQDLTERSDV LRGYLDYDAK KERLEEVNAE LEQPDVWNEP ERAQALGKER SSLEAVVDTL
0071 DQMKQGLEDV SGLLELAVEA DDEETFNEAV AELDALEEKL AQLEFRRMFS GEYDSADCYL DIQAGSGGTE
0141 AQDWASMLER MYLRWAESRG FKTEIIEESE GEVAGIKSVT IKISGDYAYG WLRTETGVHR LVRKSPFDSG
0211 GRRHTSFSSA FVYPEVDDDI DIEINPADLR IDVYRTSGAG GQHVNRTESA VRITHIPTGI VTQCQNDRSQ
0281 HKNKDQAMKQ MKAKLYELEM QKKNAEKQAM EDNKSDIGWG SQIRSYVLDD SRIKDLRTGV ETRNTQAVLD
0351 GSLDQFIEAS LKAGL
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 gccaatttag cgtcatcttc tctataaaaa
-070 agagcgtgga ttgggtacaa tcccgctctt atcaccgcat tttgactagc tcaataaaag aaatcagacc
0001 atgtttgaaa ttaatccggt aaataatcgc attcaggacc tcacggaacg ctccgacgtt cttagggggt
0071 atctttgact acgacgccaa gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct
0141 ggaacgaacc cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg tcgacaccct
0211 cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg ctgtagaagc tgacgacgaa
0281 gaaaccttta acgaagccgt tgctgaactc gacgccctgg aagaaaaact ggcgcagctt gagttccgcc
0351 gtatgttctc tggcgaatat gacagcgccg actgctacct cgatattcag gcggggtctg gcggtacgga
0421 agcacaggac tgggcgagca tgcttgagcg tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact
0491 gaaatcatcg aagagtcgga aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt
0561 acgcttacgg ctggctgcgt acagaaaccg gcgttcaccg cctggtgcgt aaaagcccgt ttgactccgg
0631 cggtcgtcgc cacacgtcgt tcagctccgc gtttgtttat ccggaagttg atgatgatat tgatatcgaa
0701 atcaacccgg cggatctgcg cattgacgtt tatcgcacgt ccggcgcggg cggtcagcac gttaaccgta
0771 ccgaatctgc ggtgcgtatt acccacatcc cgaccgggat cgtgacccag tgccagaacg accgttccca
0841 gcacaagaac aaagatcagg ccatgaagca gatgaaagcg aagctttatg aactggagat gcagaagaaa
0911 aatgccgaga aacaggcgat ggaagataac aaatccgaca tcggctgggg cagccagatt cgttcttatg
0981 tccttgatga ctcccgcatt aaagatctgc gcaccggggt agaaacccgc aacacgcagg ccgtgctgga
1051 cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag gaaccaacat gtctgaacaa
1121 cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg aactgaaaac gcgtcgtgag aagctggcga
1191 acctgcgcg
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