|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
|
Deletion
|
OCL44,45-11
(D)
,
OCL44,45-15 (AK1-9 / BK2-9)
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
thyA
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
thyA
Search strains by all related name:
thyA b2827 ECK2823 JW2795
|
General information (Go to Linear View: )
|
|
Gene Name
|
thyA
|
|
Alternative name
|
b2827,ECK2823,JW2795
|
|
Location, Length
|
2,962,383 - 2,963,177
(
-
)
;
63.85
min
;
795
(bp) ,
264
(aa)
|
|
Product
|
thymidylate synthetase
|
|
Operon Name
|
lgt-thyA
|
|
Note
|
GO_component: GO:0005737 - cytoplasm; GO_process: GO:0015949 - nucleobase, nucleoside and nucleotide interconversion
|
|
Function
|
enzyme; 2'-Deoxyribonucleotide metabolism
|
|
Gene Ontology
|
GO:0003723;
RNA binding ( thyA )
GO:0004799;
thymidylate synthase activity ( thyA )
GO:0005737;
cytoplasm ( thyA )
GO:0006231;
dTMP biosynthetic process ( thyA )
GO:0006417;
regulation of translation ( thyA )
GO:0008168;
methyltransferase activity ( thyA )
GO:0009165;
nucleotide biosynthetic process ( thyA )
GO:0016740;
transferase activity ( thyA )
|
|
PID
|
1789191
|
EC number
(KEGG Pathway)
|
2.1.1.45
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
thyA
(
EG11002
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
)
|
Entry name(Acc.no)
|
TYSY_ECOLI
(
P00470
)
|
|
- Protein name
|
Thymidylate synthase
|
|
- Synonyms
|
EC 2.1.1.45, TS, TSase
|
|
- Gene name
|
Name=thyA; OrderedLocusNames=b2827, c3422, z4144, ECs3684;
|
|
MMBR References
|
J Bacteriol. 1984;157(1):21-7.
Physical and biochemical analysis of the cloned recB and recC genes of Escherichia coli K-12.
Dykstra CC, Prasher D, Kushner SR.
(
6317651
)
|
J Bacteriol. 1984;157(1):126-9.
Suppression by thymidine-requiring mutants of Escherichia coli K-12.
Herrington MB, Kohli A, Lapchak PH.
(
6360992
)
|
Gene. 1982;18(3):257-60.
Molecular cloning and amplification of the gene for thymidylate synthetase of E. coli.
Hickson ID, Atkinson KE, Emmerson PT.
(
6290329
)
|
Proc Natl Acad Sci U S A. 1983;80:4914-4918
Primary structure of the Escherichia coli thyA gene and its thymidylate synthase product.
Belfort, M., G. Maley, J. Pederson-Lane, and F. Maley.
|
Biochem J. 1966;101:32-33.
A suggested mechanism for the selective procedure for isolating thymine-requiring mutants of Escherichia coli.
Bertino, J. B., and K. A. Stacey.
|
Gene. 1980;10(3):227-35.
Expression of thymidylate synthetase activity in Bacillus subtilis upon integration of a cloned gene from Escherichia coli.
Rubin EM, Wilson GA, Young FE.
(
6254841
)
|
Biochem Biophys Res Commun. 1982;109(2):414-22.
Fine structure of the recB and recC gene region of Escherichia coli.
Sasaki M, Fujiyoshi T, Shimada K, Takagi Y.
(
6295393
)
|
J Bacteriol. 1967;94(5):1804
Selection of thymine-requiring strains from Escherichia coli on solid medium.
Caster JH.
(
4863985
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MKQYLELMQK VLDEGTQKND RTGTGTLSIF GHQMRFNLQD GFPLVTTKRC HLRSIIHELL WFLQGDTNIA
0071 YLHENNVTIW DEWADENGDL GPVYGKQWRA WPTPDGRHID QITTVLNQLK NDPDSRRIIV SAWNVGELDK
0141 MALAPCHAFF QFYVADGKLS CQLYQRSCDV FLGLPFNIAS YALLVHMMAQ QCDLEVGDFV WTGGDTHLYS
0211 NHMDQTHLQL SREPRPLPKL IIKRKPESIF DYRFEDFEIE GYDPHPGIKA PVAI
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 catggggcaa attctttcca tcccgatgat
-070 tgtcgcgggt gtgatcatga tggtctgggc atatcgtcgc agcccacagc aacacgtttc ctgaggaacc
0001 atgaaacagt atttagaact gatgcaaaaa gtgctcgacg aaggcacaca gaaaaacgac cgtaccggaa
0071 ccggaacgct ttccattttt ggtcatcaga tgcgttttaa cctgcaagat ggattcccgc tggtgacaac
0141 taaacgttgc cacctgcgtt ccatcatcca tgaactgctg tggtttctgc agggcgacac taacattgct
0211 tatctacacg aaaacaatgt caccatctgg gacgaatggg ccgatgaaaa cggcgacctc gggccagtgt
0281 atggtaaaca gtggcgcgcc tggccaacgc cagatggtcg tcatattgac cagatcacta cggtactgaa
0351 ccagctgaaa aacgacccgg attcgcgccg cattattgtt tcagcgtgga acgtaggcga actggataaa
0421 atggcgctgg caccgtgcca tgcattcttc cagttctatg tggcagacgg caaactctct tgccagcttt
0491 atcagcgctc ctgtgacgtc ttcctcggcc tgccgttcaa cattgccagc tacgcgttat tggtgcatat
0561 gatggcgcag cagtgcgatc tggaagtggg tgattttgtc tggaccggtg gcgacacgca tctgtacagc
0631 aaccatatgg atcaaactca tctgcaatta agccgcgaac cgcgtccgct gccgaagttg attatcaaac
0701 gtaaacccga atccatcttc gactaccgtt tcgaagactt tgagattgaa ggctacgatc cgcatccggg
0771 cattaaagcg ccggtggcta tctaattacg aaacatcctg ccagagccga cgccagtgtg cgtcggtttt
0841 tttaccctcc gttaaattct tcgagacgcc ttcccgaaat tttgcaacgt cctgc
|
