MG1655
W3110
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Gene Report : thyA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCL44,45-11 (D)  ,  OCL44,45-15 (AK1-9 / BK2-9) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  thyA
Related strains
     Strains Search Search strains by gene name:  thyA   Search strains by all related name:  thyA b2827 ECK2823 JW2795

General information  (Go to Linear View:)
 Gene Name thyA  
 Alternative name b2827,ECK2823,JW2795  
 Location, Length 2,962,383 - 2,963,177 (  -  ) ;   63.85 min ; 795 (bp) ,   264 (aa) Go to Linear View
 Product thymidylate synthetase  
 Operon Name lgt-thyA  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0015949 - nucleobase, nucleoside and nucleotide interconversion  
 Function enzyme; 2'-Deoxyribonucleotide metabolism  
 Gene Ontology GO:0003723; RNA binding ( thyA )
GO:0004799; thymidylate synthase activity ( thyA )
GO:0005737; cytoplasm ( thyA )
GO:0006231; dTMP biosynthetic process ( thyA )
GO:0006417; regulation of translation ( thyA )
GO:0008168; methyltransferase activity ( thyA )
GO:0009165; nucleotide biosynthetic process ( thyA )
GO:0016740; transferase activity ( thyA )
 PID 1789191  
 EC number
  (KEGG Pathway)
 
2.1.1.45  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene thyA ( EG11002 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) TYSY_ECOLI ( P00470 )
    -  Protein name Thymidylate synthase  
    -  Synonyms EC 2.1.1.45, TS, TSase  
    -  Gene name Name=thyA; OrderedLocusNames=b2827, c3422, z4144, ECs3684;  

 Linear View (Whole Mode)
View Location
   2950.0  –  2975.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP thyA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain , Thymidylate Synthase (E.C.2.1.1.45)  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Thymidylate synthase (TS) (TSase)  
         PSI-BLAST Thymidylate synthase
    nr     (database updated : 2007.02.20 )
         BLAST Chain A, Thymidylate Synthase Complexed With Dgmp And Folate Analog 1843u89  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    THYMIDYLATE_SYNTHASE     

Other Cross-Reference
    EcoCyc thyA 

MMBR References
J Bacteriol. 1984;157(1):21-7.
Physical and biochemical analysis of the cloned recB and recC genes of Escherichia coli K-12.
Dykstra CC, Prasher D, Kushner SR. ( 6317651 )
J Bacteriol. 1984;157(1):126-9.
Suppression by thymidine-requiring mutants of Escherichia coli K-12.
Herrington MB, Kohli A, Lapchak PH. ( 6360992 )
Gene. 1982;18(3):257-60.
Molecular cloning and amplification of the gene for thymidylate synthetase of E. coli.
Hickson ID, Atkinson KE, Emmerson PT. ( 6290329 )
Proc Natl Acad Sci U S A. 1983;80:4914-4918
Primary structure of the Escherichia coli thyA gene and its thymidylate synthase product.
Belfort, M., G. Maley, J. Pederson-Lane, and F. Maley.
Biochem J. 1966;101:32-33.
A suggested mechanism for the selective procedure for isolating thymine-requiring mutants of Escherichia coli.
Bertino, J. B., and K. A. Stacey.
Gene. 1980;10(3):227-35.
Expression of thymidylate synthetase activity in Bacillus subtilis upon integration of a cloned gene from Escherichia coli.
Rubin EM, Wilson GA, Young FE. ( 6254841 )
Biochem Biophys Res Commun. 1982;109(2):414-22.
Fine structure of the recB and recC gene region of Escherichia coli.
Sasaki M, Fujiyoshi T, Shimada K, Takagi Y. ( 6295393 )
J Bacteriol. 1967;94(5):1804
Selection of thymine-requiring strains from Escherichia coli on solid medium.
Caster JH. ( 4863985 )

Sequences
Amino acid
FASTA format
0001 MKQYLELMQK VLDEGTQKND RTGTGTLSIF GHQMRFNLQD GFPLVTTKRC HLRSIIHELL WFLQGDTNIA 
0071 YLHENNVTIW DEWADENGDL GPVYGKQWRA WPTPDGRHID QITTVLNQLK NDPDSRRIIV SAWNVGELDK 
0141 MALAPCHAFF QFYVADGKLS CQLYQRSCDV FLGLPFNIAS YALLVHMMAQ QCDLEVGDFV WTGGDTHLYS 
0211 NHMDQTHLQL SREPRPLPKL IIKRKPESIF DYRFEDFEIE GYDPHPGIKA PVAI

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             catggggcaa attctttcca tcccgatgat 
-070 tgtcgcgggt gtgatcatga tggtctgggc atatcgtcgc agcccacagc aacacgtttc ctgaggaacc 
0001 atgaaacagt atttagaact gatgcaaaaa gtgctcgacg aaggcacaca gaaaaacgac cgtaccggaa 
0071 ccggaacgct ttccattttt ggtcatcaga tgcgttttaa cctgcaagat ggattcccgc tggtgacaac 
0141 taaacgttgc cacctgcgtt ccatcatcca tgaactgctg tggtttctgc agggcgacac taacattgct 
0211 tatctacacg aaaacaatgt caccatctgg gacgaatggg ccgatgaaaa cggcgacctc gggccagtgt 
0281 atggtaaaca gtggcgcgcc tggccaacgc cagatggtcg tcatattgac cagatcacta cggtactgaa 
0351 ccagctgaaa aacgacccgg attcgcgccg cattattgtt tcagcgtgga acgtaggcga actggataaa 
0421 atggcgctgg caccgtgcca tgcattcttc cagttctatg tggcagacgg caaactctct tgccagcttt 
0491 atcagcgctc ctgtgacgtc ttcctcggcc tgccgttcaa cattgccagc tacgcgttat tggtgcatat 
0561 gatggcgcag cagtgcgatc tggaagtggg tgattttgtc tggaccggtg gcgacacgca tctgtacagc 
0631 aaccatatgg atcaaactca tctgcaatta agccgcgaac cgcgtccgct gccgaagttg attatcaaac 
0701 gtaaacccga atccatcttc gactaccgtt tcgaagactt tgagattgaa ggctacgatc cgcatccggg 
0771 cattaaagcg ccggtggcta tctaattacg aaacatcctg ccagagccga cgccagtgtg cgtcggtttt 
0841 tttaccctcc gttaaattct tcgagacgcc ttcccgaaat tttgcaacgt cctgc