|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
J Bacteriol. 1985;163(3):1055-9.
Physical characterization of the cloned protease III gene from Escherichia coli K-12.
Dykstra CC, Kushner SR.
(
2993229
)
|
|
|
Deletion
|
DE(argA-thyA)232
,
OCL44,45-11
(D)
,
OCL44,45-15 (AK1-9 / BK2-9)
(D)
,
OCL44,45-3
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
ptrA
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
ptrA
Search strains by all related name:
ptrA b2821 ECK2817 JW2789 ptr
|
General information (Go to Linear View: )
|
|
Gene Name
|
ptrA
|
|
Alternative name
|
b2821,ECK2817,JW2789,ptr
|
|
Location, Length
|
2,954,018 - 2,956,906
(
-
)
;
63.67
min
;
2889
(bp) ,
962
(aa)
|
|
Product
|
protease III
|
|
Operon Name
|
ptrA-recBD
|
|
Note
|
|
|
Function
|
enzyme; Degradation of proteins, peptides, glyco
|
|
Gene Ontology
|
GO:0000287
;
magnesium ion binding ( ptrA )
GO:0003824
;
catalytic activity ( ptrA )
GO:0004222
;
metalloendopeptidase activity ( ptrA )
GO:0006508
;
proteolysis ( ptrA )
GO:0008233
;
peptidase activity ( ptrA )
GO:0008237
;
metallopeptidase activity ( ptrA )
GO:0008270
;
zinc ion binding ( ptrA )
GO:0008975
;
pitrilysin activity ( ptrA )
GO:0016787
;
hydrolase activity ( ptrA )
GO:0042597
;
periplasmic space ( ptrA )
GO:0046872
;
metal ion binding ( ptrA )
|
|
PID
|
2367164
|
EC number
(KEGG Pathway)
|
3.4.24.55
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
PTRA_ECOLI
(
P05458
;
P78106
)
|
|
- Protein name
|
Protease III precursor
|
|
- Synonyms
|
EC 3.4.24.55, Pitrilysin, Protease pi
|
|
- Gene name
|
Name=ptrA; Synonyms=ptr; OrderedLocusNames=b2821;
|
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Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
ptr
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of Pitrilysin
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Protease 3 precursor
|
|
PSI-BLAST
|
Protease 3 precursor
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
protease III [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
INSULINASE
|
|
MMBR References
|
J Bacteriol. 1985;163(3):1055-9.
Physical characterization of the cloned protease III gene from Escherichia coli K-12.
Dykstra CC, Kushner SR.
(
2993229
)
|
J Bacteriol. 1984;157(1):21-7.
Physical and biochemical analysis of the cloned recB and recC genes of Escherichia coli K-12.
Dykstra CC, Prasher D, Kushner SR.
(
6317651
)
|
Nucleic Acids Res. 1986;14:7695-7703
Complete nucleotide sequence of Escherichia coli ptr gene encoding protease III.
Finch, P. W., R. E. Wilson, K. Brown, I. D. Hickson, and P. T. Emmerson.
|
J Bacteriol. 1987;169(9):4076-85.
Identification of polypeptides encoded by an Escherichia coli locus (hflA) that governs the lysis-lysogeny decision of bacteriophage lambda.
Banuett F, Herskowitz I.
(
3040675
)
|
J Bacteriol. 1979;140(1):125-30.
Isolation and characterization of mutations in the structural gene for protease III (ptr).
Cheng YS, Zipser D, Cheng CY, Rolseth SJ.
(
387716
)
|
Gene. 1987;54(2-3):185-95.
Analysis of the regulatory region of the protease III (ptr) gene of Escherichia coli K-12.
Claverie-Martin F, Diaz-Torres MR, Kushner SR.
(
3308636
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MPRSTWFKAL LLLVALWAPL SQAETGWQPI QETIRKSDKD NRQYQAIRLD NGMVVLLVSD PQAVKSLSAL
0071 VVPVGSLEDP EAYQGLAHYL EHMSLMGSKK YPQADSLAEY LKMHGGSHNA STAPYRTAFY LEVENDALPG
0141 AVDRLADAIA EPLLDKKYAE RERNAVNAEL TMARTRDGMR MAQVSAETIN PAHPGSKFSG GNLETLSDKP
0211 GNPVQQALKD FHEKYYSANL MKAVIYSNKP LPELAKMAAD TFGRVPNKES KKPEITVPVV TDAQKGIIIH
0281 YVPALPRKVL RVEFRIDNNS AKFRSKTDEL ITYLIGNRSP GTLSDWLQKQ GLVEGISANS DPIVNGNSGV
0351 LAISASLTDK GLANRDQVVA AIFSYLNLLR EKGIDKQYFD ELANVLDIDF RYPSITRDMD YVEWLADTMI
0421 RVPVEHTLDA VNIADRYDAK AVKERLAMMT PQNARIWYIS PKEPHNKTAY FVDAPYQVDK ISAQTFADWQ
0491 KKAADIALSL PELNPYIPDD FSLIKSEKKY DHPELIVDES NLRVVYAPSR YFASEPKADV SLILRNPKAM
0561 DSARNQVMFA LNDYLAGLAL DQLSNQASVG GISFSTNANN GLMVNANGYT QRLPQLFQAL LEGYFSYTAT
0631 EDQLEQAKSW YNQMMDSAEK GKAFEQAIMP AQMLSQVPYF SRDERRKILP SITLKEVLAY RDALKSGARP
0701 EFMVIGNMTE AQATTLARDV QKQLGADGSE WCRNKDVVVD KKQSVIFEKA GNSTDSALAA VFVPTGYDEY
0771 TSSAYSSLLG QIVQPWFYNQ LRTEEQLGYA VFAFPMSVGR QWGMGFLLQS NDKQPSFLWE RYKAFFPTAE
0841 AKLRAMKPDE FAQIQQAVIT QMLQAPQTLG EEASKLSKDF DRGNMRFDSR DKIVAQIKLL TPQKLADFFH
0911 QAVVEPQGMA ILSQISGSQN GKAEYVHPEG WKVWENVSAL QQTMPLMSEK NE
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 cataggctga ctcgtgcagc acaagattaa
-070 attctggcag atgatttgcg ttaacgtgtt gaatctggac agaaaattaa gttgattatg aggtccgtga
0001 atgccccgca gcacctggtt caaagcatta ttgttgttag ttgccctttg ggcaccctta agtcaggcag
0071 aaacgggatg gcagccgatt caggaaacca tccgtaaaag tgataaagat aaccgccagt atcaggctat
0141 acgtctggat aacggtatgg tggtcttgct ggtttctgat ccgcaggcag ttaaatcgct ctcggcgctg
0211 gtggtgcccg ttgggtcgct ggaagatccc gaggcgtacc aggggctggc acattacctt gaacatatga
0281 gtctgatggg gtcgaaaaag tacccgcagg ctgacagtct ggccgaatat ctcaaaatgc acggcggtag
0351 tcacaatgcc agcactgcgc cgtatcgcac ggctttctat ctggaagttg agaacgacgc cttgcctggt
0421 gcggtagacc gcctggccga tgctattgct gaacctttgc tcgacaagaa atatgccgaa cgtgagcgta
0491 atgcggtgaa cgctgaatta accatggcgc gtacgcgtga cgggatgcgc atggcacagg tcagcgcaga
0561 aaccattaac ccggcacacc ccggttcaaa gttttctggt ggtaacctcg aaactttaag cgacaaacct
0631 ggtaatccgg tgcagcaggc gctgaaagat ttccacgaga agtactattc cgccaatttg atgaaggcgg
0701 ttatttacag taataaaccg ctgccggagt tggcaaaaat ggcggcggac acctttggtc gcgtgccgaa
0771 caaagagagc aaaaaaccgg aaatcaccgt gccggtagtc accgacgcgc aaaagggcat tatcattcat
0841 tacgtccctg cgctgccgcg taaagtgttg cgcgttgagt ttcgcatcga taacaactca gcgaagttcc
0911 gtagtaaaac cgatgaattg attacctatc tgattggcaa tcgcagccca ggtacacttt ctgactggct
0981 gcaaaagcag ggattagttg agggcattag cgccaactcc gatcctatcg tcaacggcaa cagcggcgta
1051 ttagcgatct ctgcgtcttt aaccgataaa ggcctggcta atcgcgatca ggttgtggcg gcaattttta
1121 gctatctcaa tctgttacgt gaaaaaggca ttgataaaca atacttcgat gaactggcga atgtgctgga
1191 tatcgacttc cgttatccgt cgatcacccg tgatatggat tacgtcgaat ggctggcaga taccatgatt
1261 cgcgttcctg ttgagcatac gctggatgca gtcaatattg ccgatcggta cgatgctaaa gcagtaaagg
1331 aacgtctggc gatgatgacg ccgcagaatg cgcgtatctg gtatatcagc ccgaaagagc cgcacaacaa
1401 aacggcttac tttgtcgatg cgccgtatca ggtcgataaa atcagcgcac aaactttcgc cgactggcag
1471 aaaaaagccg ccgacattgc gctctctttg ccagagctta acccttatat tcctgatgat ttctcgctga
1541 ttaagtcaga gaagaaatac gaccatccag agctgattgt tgatgagtcg aatctgcgcg tggtgtatgc
1611 gccaagccgt tattttgcca gcgagcccaa agctgatgtc agcctgattt tgcgtaatcc gaaagccatg
1681 gacagcgccc gcaatcaggt gatgtttgcg ctcaatgatt atctcgcagg gctggcgctt gatcagttaa
1751 gcaaccaggc gtcggttggt ggcataagtt tttccaccaa cgctaacaac ggccttatgg ttaatgctaa
1821 tggttacacc cagcgtctgc cgcagctgtt ccaggcattg ctcgaggggt actttagcta taccgctacg
1891 gaagatcagc ttgagcaggc gaagtcctgg tataaccaga tgatggattc cgcagaaaag ggtaaagcgt
1961 ttgagcaggc gattatgccc gcgcagatgc tctcgcaagt gccgtacttc tcgcgagatg aacggcgtaa
2031 aattttgccc tccattacgt tgaaagaggt gctggcctat cgcgacgcct taaaatcagg ggctcgacca
2101 gagtttatgg ttatcggcaa catgaccgag gcccaggcaa caacgctggc acgcgatgtg caaaaacagt
2171 tgggcgctga tggttcagag tggtgtcgaa acaaagatgt agtggtcgat aaaaaacaat ccgtcatctt
2241 tgaaaaagcc ggtaacagca ccgactccgc actggcagcg gtatttgtac cgactggcta cgatgaatac
2311 accagctcag cctatagctc tctgttgggg cagatcgtac agccgtggtt ctacaatcag ttgcgtaccg
2381 aagaacaatt gggctatgcc gtgtttgcgt ttccaatgag cgtggggcgt cagtggggca tgggcttcct
2451 tttgcaaagc aatgataaac agccttcatt cttgtgggag cgttacaagg cgtttttccc aaccgcagag
2521 gcaaaattgc gagcgatgaa gccagatgag tttgcgcaaa tccagcaggc ggtaattacc cagatgctgc
2591 aggcaccgca aacgctcggc gaagaagcat cgaagttaag taaagatttc gatcgcggca atatgcgctt
2661 cgattcgcgt gataaaatcg tggcccagat aaaactgctg acgccgcaaa aacttgctga tttcttccat
2731 caggcggtgg tcgagccgca aggcatggct attctgtcgc agatttccgg cagccagaac gggaaagccg
2801 aatatgtaca ccctgaaggc tggaaagtgt gggagaacgt cagcgcgttg cagcaaacaa tgcccctgat
2871 gagtgaaaag aatgagtgat gtcgccgaga cactagatcc tttgcgcttg cccttacagg gtgagcgcct
2941 gattgaagcc tctgccggca caggcaaaac ctttacgatt gcggcgctc
|
