MG1655
W3110
Search for
Japanese | English
Gene Report : recB
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion DE(argA-thyA)232  ,  OCL44,45-11 (D)  ,  OCL44,45-15 (AK1-9 / BK2-9) (D)  ,  OCL44,45-3 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  recB
Related strains
     Strains Search Search strains by gene name:  recB   Search strains by all related name:  recB b2820 ECK2816 ior JW2788 rorA

General information  (Go to Linear View:)
 Gene Name recB  
 Alternative name b2820,ECK2816,ior,JW2788,rorA  
 Location, Length 2,950,483 - 2,954,025 (  -  ) ;   63.59 min ; 3543 (bp) ,   1180 (aa) Go to Linear View
 Product exonuclease V (RecBCD complex), beta subunit  
 Operon Name ptrA-recBD  
 Note DNA helicase, ATP-dependent dsDNA/ssDNA exonuclease V subunit, ssDNA endonuclease; GO_process: GO:0006308 - DNA catabolic process; GO_process: GO:0006310 - DNA recombination  
 Function enzyme; Degradation of DNA  
 Gene Ontology GO:0000166; nucleotide binding ( recB )
GO:0003677; DNA binding ( recB )
GO:0004003; ATP-dependent DNA helicase activity ( recB )
GO:0004386; helicase activity ( recB )
GO:0004518; nuclease activity ( recB )
GO:0004519; endonuclease activity ( recB )
GO:0004527; exonuclease activity ( recB )
GO:0005524; ATP binding ( recB )
GO:0006281; DNA repair ( recB )
GO:0006974; response to DNA damage stimulus ( recB )
GO:0008854; exodeoxyribonuclease V activity ( recB )
GO:0016787; hydrolase activity ( recB )
 PID 1789183  
 EC number
  (KEGG Pathway)
 
3.1.11.5  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene recB ( EG10824 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) EX5B_ECOLI ( P08394 )
    -  Protein name Exodeoxyribonuclease V beta chain  
    -  Synonyms EC 3.1.11.5, Exodeoxyribonuclease V 135 kDa polypeptide  
    -  Gene name Name=recB; Synonyms=rorA; OrderedLocusNames=b2820;  

 Linear View (Whole Mode)
View Location
   2940.0  –  2965.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP recB (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain B, Recbcd:dna Complex  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Exodeoxyribonuclease V beta chain  
         PSI-BLAST Exodeoxyribonuclease V beta chain
    nr     (database updated : 2007.02.20 )
         BLAST exonuclease V (RecBCD complex), beta subunit [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    RGD    ATP_GTP_A    CRYSTALLIN_BETAGAMMA     

Other Cross-Reference
    COG COG1074L 
    EcoCyc recB 

MMBR References
J Bacteriol. 1984;157(1):21-7.
Physical and biochemical analysis of the cloned recB and recC genes of Escherichia coli K-12.
Dykstra CC, Prasher D, Kushner SR. ( 6317651 )
Mol Gen Genet. 1995;246(4):514-8.
An Escherichia coli strain deficient for both exonuclease V and deoxycytidine triphosphate deaminase shows enhanced sensitivity to ionizing radiation.
Estevenon AM, Kooistra J, Sicard N. ( 7891665 )
Nucleic Acids Res. 1986;14:8573-8582
Complete nucleotide sequence of the Escherichia coli recB gene.
Finch, P. W., A. Storey, K. E. Chapman, K. Brown, I. D. Hickson, and P. T. Emmerson.
Nature. 1981;294(5841):578-80.
Identification of the Escherichia coli recB and recC gene products.
Hickson ID, Emmerson PT. ( 6458768 )
Biochem Biophys Res Commun. 1982;109(2):414-22.
Fine structure of the recB and recC gene region of Escherichia coli.
Sasaki M, Fujiyoshi T, Shimada K, Takagi Y. ( 6295393 )
J Biol Chem. 1992;267:4981-4987
The RecB subunit of the Escherichia coli RecBCD enzyme couples ATP hydrolysis to DNA unwinding.
Boehmer, P. E., and P. T. Emmerson.
J Biol Chem. 1995;270:24451-24458
Monomeric RecBCD enzyme binds and unwinds DNA.
Taylor, A. F., and G. R. Smith.
J Biol Chem. 1971;246:6543-6555
The isolation and characterization of an adenosine triphosphate-dependent deoxyribonuclease directed by recB, C genes.
Wright, M., G. Bultin, and J. Hurwitz.
Mol Gen Genet. 1985;201(3):525-8.
Role of Escherichia coli RecBC enzyme in SOS induction.
Chaudhury AM, Smith GR. ( 3911029 )
Biochimie. 1991;73(4):523-32.
rec genes and homologous recombination proteins in Escherichia coli.
Clark AJ. ( 1911953 )

Sequences
Amino acid
FASTA format
0001 MSDVAETLDP LRLPLQGERL IEASAGTGKT FTIAALYLRL LLGLGGSAAF PRPLTVEELL VVTFTEAATA 
0071 ELRGRIRSNI HELRIACLRE TTDNPLYERL LEEIDDKAQA AQWLLLAERQ MDEAAVFTIH GFCQRMLNLN 
0141 AFESGMLFEQ QLIEDESLLR YQACADFWRR HCYPLPREIA QVVFETWKGP QALLRDINRY LQGEAPVIKA 
0211 PPPDDETLAS RHAQIVARID TVKQQWRDAV GELDALIESS GIDRRKFNRS NQAKWIDKIS AWAEEETNSY 
0281 QLPESLEKFS QRFLEDRTKA GGETPRHPLF EAIDQLLAEP LSIRDLVITR ALAEIRETVA REKRRRGELG 
0351 FDDMLSRLDS ALRSESGEVL AAAIRTRFPV AMIDEFQDTD PQQYRIFRRI WHHQPETALL LIGDPKQAIY 
0421 AFRGADIFTY MKARSEVHAH YTLDTNWRSA PGMVNSVNKL FSQTDDAFMF REIPFIPVKS AGKNQALRFV 
0491 FKGETQPAMK MWLMEGESCG VGDYQSTMAQ VCAAQIRDWL QAGQRGEALL MNGDDARPVR ASDISVLVRS 
0561 RQEAAQVRDA LTLLEIPSVY LSNRDSVFET LEAQEMLWLL QAVMTPEREN TLRSALATSM MGLNALDIET 
0631 LNNDEHAWDV VVEEFDGYRQ IWRKRGVMPM LRALMSARNI AENLLATAGG ERRLTDILHI SELLQEAGTQ 
0701 LESEHALVRW LSQHILEPDS NASSQQMRLE SDKHLVQIVT IHKSKGLEYP LVWLPFITNF RVQEQAFYHD 
0771 RHSFEAVLDL NAAPESVDLA EAERLAEDLR LLYVALTRSV WHCSLGVAPL VRRRGDKKGD TDVHQSALGR 
0841 LLQKGEPQDA AGLRTCIEAL CDDDIAWQTA QTGDNQPWQV NDVSTAELNA KTLQRLPGDN WRVTSYSGLQ 
0911 QRGHGIAQDL MPRLDVDAAG VASVVEEPTL TPHQFPRGAS PGTFLHSLFE DLDFTQPVDP NWVREKLELG 
0981 GFESQWEPVL TEWITAVLQA PLNETGVSLS QLSARNKQVE MEFYLPISEP LIASQLDTLI RQFDPLSAGC 
1051 PPLEFMQVRG MLKGFIDLVF RHEGRYYLLD YKSNWLGEDS SAYTQQAMAA AMQAHRYDLQ YQLYTLALHR 
1121 YLRHRIADYD YEHHFGGVIY LFLRGVDKEH PQQGIYTTRP NAGLIALMDE MFAGMTLEEA 

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             agccagaacg ggaaagccga atatgtacac 
-070 cctgaaggct ggaaagtgtg ggagaacgtc agcgcgttgc agcaaacaat gcccctgatg agtgaaaaga 
0001 atgagtgatg tcgccgagac actagatcct ttgcgcttgc ccttacaggg tgagcgcctg attgaagcct 
0071 ctgccggcac aggcaaaacc tttacgattg cggcgctcta tttgcgcctg ttacttggac taggcggttc 
0141 cgccgccttt ccccgcccgc tgaccgttga agaactgctg gtggtcacct ttaccgaggc tgccacggca 
0211 gaattgcgcg gtcgtatccg tagcaatatc cacgagttgc gcatcgcctg tctgcgtgaa accaccgaca 
0281 atccactgta cgaacgcctg ctggaagaga tcgacgataa agcgcaagcc gcgcagtggt tgttgttagc 
0351 cgaacggcag atggatgaag cggcagtctt tactattcac ggcttttgcc agcgcatgct caacctgaat 
0421 gcctttgaat ccggcatgct gtttgagcag cagctgattg aagatgagtc tctgctacgc taccaggcct 
0491 gcgccgattt ctggcgtcgc cactgctacc cgctgccgcg tgaaatagcc caggtcgtct ttgaaacctg 
0561 gaaagggccg caggcgttgc tgcgcgatat taatcgttat ctgcaaggcg aagcgccggt tatcaaagca 
0631 ccgccgcccg atgatgaaac gctggcttcc cgtcacgcgc aaattgtggc gcgtattgat acggtaaaac 
0701 agcagtggcg cgacgcagtg ggtgaactgg atgcgctgat cgaatcttct ggtattgatc gacgcaagtt 
0771 taaccgtagc aatcaggcta aatggatcga caagatcagc gcctgggcag aagaagagac aaacagttat 
0841 cagttgccgg agtcgctgga aaaattctcc cagcgtttct tagaagatcg cacgaaggcc gggggggaaa 
0911 ccccgcgaca tccactgttt gaggcgatcg atcaactgct tgcagaacca ttgtcgatcc gcgatctggt 
0981 gatcacccgc gcattggctg agatccgcga aacagtagcg cgtgaaaaac gccgccgtgg cgaattgggt 
1051 tttgatgaca tgttaagtcg gctcgattcc gcgctgcgta gcgaaagcgg tgaggtgttg gcagcggcga 
1121 tccgtacgcg attcccggtg gcaatgatcg atgaatttca ggataccgac ccccagcagt accgaatttt 
1191 tcgccgtatc tggcaccatc agccggaaac cgcattgttg ctaattggcg acccgaagca ggccatatat 
1261 gcattccggg gtgcggatat cttcacttat atgaaggcgc gtagcgaagt tcacgcccac tacactttag 
1331 acaccaactg gcgttccgca ccaggaatgg tgaacagcgt gaataagctt ttcagccaga ctgatgacgc 
1401 gttcatgttt cgcgaaatac cgtttattcc agtgaaatca gccgggaaaa atcaggcgtt acgttttgta 
1471 tttaaaggtg aaacacagcc tgcgatgaaa atgtggctga tggaaggcga aagctgcggc gttggcgatt 
1541 atcaaagtac catggcgcag gtatgtgctg cgcaaatccg cgactggcta caagccggac agcggggcga 
1611 agcgttgctg atgaacggcg acgacgcgcg tccggtgcgt gcttcggaca tcagtgtgct ggtgcgcagc 
1681 cgccaggagg ccgcccaggt gcgcgatgcc ttaacgttgc tggaaatccc ttccgtttac ctttcgaacc 
1751 gcgacagtgt ttttgaaact ctggaagcgc aggaaatgct ttggttgttg caggcggtga tgacgcccga 
1821 acgtgagaac accctgcgta gtgcgctggc aacgtcaatg atggggctga acgcgctgga tatcgaaacg 
1891 ctgaacaatg acgaacatgc gtgggatgtg gtagtcgaag agttcgatgg ttatcggcaa atctggcgca 
1961 aacgtggcgt tatgccgatg ctgcgggcgc tgatgtcggc gcgtaacatt gctgaaaact tgctggcaac 
2031 ggcaggcggt gagcggcgtc ttaccgatat cttgcatatc agcgaactgc tacaagaagc cggaacgcag 
2101 ctggaaagtg aacatgcgct ggtacgctgg ttatcgcaac atatcctcga gccagacagt aatgcctcca 
2171 gccaacaaat gcgtctcgaa agtgataaac atctggtgca gattgtcacg atccacaaat cgaaagggct 
2241 ggaatatcca ttggtctggc tgccgtttat caccaatttc cgcgtccagg agcaggcgtt ttatcacgat 
2311 cgccactcgt ttgaggcagt tctggatctt aatgctgcgc cagaaagcgt cgacctcgcg gaggccgaac 
2381 gtctggcgga agatctgcgt ttgctttacg tggcgctgac acgttcggtt tggcattgca gtctcggcgt 
2451 tgcaccgctg gtgcgccgtc gtggcgataa aaaaggtgac accgacgtcc accaaagtgc gctcgggcgt 
2521 ttgctgcaaa aaggggaacc gcaagatgcg gcagggcttc gcacctgtat tgaagcgtta tgcgatgatg 
2591 atattgcctg gcaaacggca caaactggtg ataaccaacc ctggcaggtt aatgatgttt ctacagcaga 
2661 gctgaatgcg aagacgttac aacgattgcc cggcgataac tggcgcgtca ccagctactc tggtttgcaa 
2731 cagcgtggtc acggtatcgc ccaggatttg atgcctcggc tggatgtcga tgctgcaggc gttgccagcg 
2801 tcgttgaaga accgacgtta acaccacatc agtttccgcg cggtgcgtca ccggggacgt tcttgcacag 
2871 tttgtttgaa gacctggatt ttacccagcc ggttgacccg aactgggtgc gggaaaaact ggaactcggc 
2941 ggctttgaat cgcagtggga accggtattg accgagtgga tcacggctgt cctccaggca cctctcaatg 
3011 aaaccggcgt aagcctgagt caactttccg cccgcaataa acaggtggag atggagtttt atctgccgat 
3081 tagtgaaccg cttatcgcca gtcagcttga tacgttaatc cgccagtttg acccgctatc cgcaggctgc 
3151 ccgccgctgg agttcatgca ggtacgtggc atgttaaaag gctttatcga cctggtgttc cgccacgaag 
3221 ggcgttatta cctgctcgac tataaatcca actggttggg tgaagacagt tcggcttaca cccaacaggc 
3291 tatggcagcg gcaatgcagg cacaccgcta tgatctgcaa tatcagcttt ataccctggc gctgcatcgt 
3361 tatctgcgcc atcgcattgc tgattacgac tatgagcacc actttggcgg cgttatttat ctgttcctgc 
3431 gtggcgttga taaagaacat ccgcaacagg ggatttacac aacccgaccc aacgccgggt tgattgccct 
3501 gatggatgag atgtttgccg gtatgaccct ggaggaggcg taatgaaatt gcaaaagcaa ttactggaag 
3571 ctgtggagca caaacagcta cgcccgctgg atgtgcaatt tgccctgacc gtggcgggag atgaacatcc 
3641 tgc