MG1655
W3110
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Gene Report : recD
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion DE(argA-thyA)232  ,  OCL44,45-11 (D)  ,  OCL44,45-15 (AK1-9 / BK2-9) (D)  ,  OCL44,45-3 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  recD
Related strains
     Strains Search Search strains by gene name:  recD   Search strains by all related name:  recD b2819 ECK2815 hopE JW2787

General information  (Go to Linear View:)
 Gene Name recD  
 Alternative name b2819,ECK2815,hopE,JW2787  
 Location, Length 2,948,657 - 2,950,483 (  -  ) ;   63.55 min ; 1827 (bp) ,   608 (aa) Go to Linear View
 Product exonuclease V (RecBCD complex), alpha chain  
 Operon Name ptrA-recBD  
 Note DNA helicase, ATP-dependent dsDNA/ssDNA exonuclease V subunit, ssDNA endonuclease; GO_process: GO:0006308 - DNA catabolic process; GO_process: GO:0006310 - DNA recombination  
 Function enzyme; Degradation of DNA  
 Gene Ontology GO:0000166; nucleotide binding ( recD )
GO:0004386; helicase activity ( recD )
GO:0004518; nuclease activity ( recD )
GO:0004519; endonuclease activity ( recD )
GO:0004527; exonuclease activity ( recD )
GO:0005524; ATP binding ( recD )
GO:0006281; DNA repair ( recD )
GO:0006974; response to DNA damage stimulus ( recD )
GO:0008854; exodeoxyribonuclease V activity ( recD )
GO:0009338; exodeoxyribonuclease V complex ( recD )
GO:0016787; hydrolase activity ( recD )
GO:0017111; nucleoside-triphosphatase activity ( recD )
 PID 1789182  
 EC number
  (KEGG Pathway)
 
3.1.11.5  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene recD ( EG10826 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) EX5A_ECOLI ( P04993 ; Q59378 )
    -  Protein name Exodeoxyribonuclease V alpha chain  
    -  Synonyms EC 3.1.11.5, Exodeoxyribonuclease V 67 kDa polypeptide  
    -  Gene name Name=recD; OrderedLocusNames=b2819;  

 Linear View (Whole Mode)
View Location
   2940.0  –  2965.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP recD (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain D, Recbcd:dna Complex  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Exodeoxyribonuclease V alpha chain  
         PSI-BLAST UvrABC system protein B
    nr     (database updated : 2007.02.20 )
         BLAST exonuclease V (RecBCD complex), alpha chain [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    RGD    ATP_GTP_A     

Other Cross-Reference
    COG COG0507L 
    EcoCyc recD 

MMBR References
Nucleic Acids Res. 1986;14:8583-8594
Complete nucleotide sequence of recD, the structural gene for the alpha subunit of exonuclease V of Escherichia coli.
Finch, P. W., A. Storey, K. Brown, I. D. Hickson, and P. T. Emmerson.
Mol Gen Genet. 1990;224(1):1-9.
Linear multimer formation of plasmid DNA in Escherichia coli hopE (recD) mutants.
Niki H, Ogura T, Hiraga S. ( 2177520 )
J Bacteriol. 1986;167(2):594-603.
Identification and characterization of recD, a gene affecting plasmid maintenance and recombination in Escherichia coli.
Biek DP, Cohen SN. ( 3015881 )
J Biol Chem. 1995;270:24451-24458
Monomeric RecBCD enzyme binds and unwinds DNA.
Taylor, A. F., and G. R. Smith.
Biochimie. 1991;73(4):523-32.
rec genes and homologous recombination proteins in Escherichia coli.
Clark AJ. ( 1911953 )
Proc Natl Acad Sci U S A. 1986;83:5558-5562
recD: the gene for an essential third subunit of exonuclease V.
Amundsen, S. K., A. F. Taylor, A. M. Chaudhury, and G. R. Smith.

Sequences
Amino acid
FASTA format
0001 MKLQKQLLEA VEHKQLRPLD VQFALTVAGD EHPAVTLAAA LLSHDAGEGH VCLPLSRLEN NEASHPLLAT 
0071 CVSEIGELQN WEECLLASQA VSRGDEPTPM ILCGDRLYLN RMWCNERTVA RFFNEVNHAI EVDEALLAQT 
0141 LDKLFPVSDE INWQKVAAAV ALTRRISVIS GGPGTGKTTT VAKLLAALIQ MADGERCRIR LAAPTGKAAA 
0211 RLTESLGKAL RQLPLTDEQK KRIPEDASTL HRLLGAQPGS QRLRHHAGNP LHLDVLVVDE ASMIDLPMMS 
0281 RLIDALPDHA RVIFLGDRDQ LASVEAGAVL GDICAYANAG FTAERARQLS RLTGTHVPAG TGTEAASLRD 
0351 SLCLLQKSYR FGSDSGIGQL AAAINRGDKT AVKTVFQQDF TDIEKRLLQS GEDYIAMLEE ALAGYGRYLD 
0421 LLQARAEPDL IIQAFNEYQL LCALREGPFG VAGLNERIEQ FMQQKRKIHR HPHSRWYEGR PVMIARNDSA 
0491 LGLFNGDIGI ALDRGQGTRV WFAMPDGNIK SVQPSRLPEH ETTWAMTVHK SQGSEFDHAA LILPSQRTPV 
0561 VTRELVYTAV TRARRRLSLY ADERILSAAI ATRTERRSGL AALFSSRE

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             aagaacatcc gcaacagggg atttacacaa 
-070 cccgacccaa cgccgggttg attgccctga tggatgagat gtttgccggt atgaccctgg aggaggcgta 
0001 atgaaattgc aaaagcaatt actggaagct gtggagcaca aacagctacg cccgctggat gtgcaatttg 
0071 ccctgaccgt ggcgggagat gaacatcctg ccgtcaccct cgcggcggca ctgttaagtc atgatgccgg 
0141 agagggacac gtttgtttgc cgctttcacg actggaaaat aacgaggcgt cgcatccgct gttggcgacc 
0211 tgtgtcagtg aaatcggtga gctacaaaat tgggaagaat gcttgctggc ttctcaagcg gtcagcaggg 
0281 gagatgaacc cacgccgatg atcctctgtg gcgatcgtct ttatttgaat cgcatgtggt gtaacgagcg 
0351 cacagtggca cgctttttca acgaagtgaa tcatgccatt gaggttgatg aagctctact ggcgcaaacc 
0421 ctggacaaac tttttccagt aagcgatgaa attaactggc aaaaagttgc ggcggcagtg gcgctgacgc 
0491 ggcggatctc ggtgatttcc ggcggccctg gcaccggtaa aacgaccacc gtagcgaagt tgctggcagc 
0561 gttaattcaa atggccgacg gcgaacgctg ccgtatccgt ctggctgcac caacgggtaa agctgccgcg 
0631 cgcttaaccg aatctctcgg caaggctttg cgacagttac cgctgaccga tgaacaaaag aaacgcattc 
0701 cggaagatgc cagcactttg caccgattgc tgggcgcgca gccgggtagc cagcgtttac gtcatcatgc 
0771 cggtaacccg ctgcatcttg atgtgctggt ggtagatgaa gcgtcaatga tcgatctgcc tatgatgtcg 
0841 agactgatcg acgccttgcc cgatcatgcg cgagtgatct ttctcggcga tcgtgatcaa ctggcctcgg 
0911 ttgaggctgg ggctgtgctg ggcgatatct gcgcttatgc caacgcgggc tttaccgccg agcgtgccag 
0981 gcagctaagc cgcctgacgg ggactcacgt tccggcagga accggcacag aagcggcatc tttgcgcgac 
1051 agtctctgcc tgctgcaaaa aagctatcgt ttcggcagcg attctggcat tggtcagtta gctgcggcga 
1121 tcaaccgtgg tgataaaacg gcagtgaaaa ccgtttttca gcaggatttt actgatatcg aaaaacggct 
1191 tttacagagc ggcgaagatt atattgcgat gcttgaggaa gctcttgcgg gttacggacg ttatctggat 
1261 ctgctgcaag cgcgtgccga gccggattta atcattcagg cgttcaatga gtaccagctt ttgtgcgccc 
1331 tgcgggaagg gccgtttggc gtggctggac tgaatgagcg aattgagcag tttatgcaac agaagcgcaa 
1401 aattcatcgt catccgcact ctcgttggta cgaaggtcga ccggtgatga ttgcccgtaa tgacagcgcg 
1471 cttgggttgt ttaatggcga tatcggtatt gcgctggatc gcgggcaggg gacgcgcgtc tggtttgcga 
1541 tgccggacgg caatattaag tctgtgcaac cgagtcgctt gccagagcac gaaactacgt gggcgatgac 
1611 ggtacataaa tcgcagggat cggagttcga ccatgcggcg ttgattttgc cgagccaacg cacgccggta 
1681 gtaacgcgag agctggttta taccgcggtg acccgcgcgc gtcgccgtct gtcgctgtat gccgatgagc 
1751 gcatattaag tgcggcaatc gccactcgta ctgagcggcg cagtggtctg gcggcgttgt ttagttcacg 
1821 ggaataaacg taattgccgg atgcgacgta cgagtgttac gcatgtcgca tccgacgatt ttcatcgctt 
1891 accctaaatc cgccatcaac actttggatt tacgctg