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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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LD3-18-1
(LD)
,
OCL44,45-1
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
relA
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Related strains
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Strains Search
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Search strains by gene name:
relA
Search strains by all related name:
relA b2784 ECK2778 JW2755 RC
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General information (Go to Linear View: )
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Gene Name
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relA
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Alternative name
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b2784,ECK2778,JW2755,RC
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Location, Length
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2,909,439 - 2,911,673
(
-
)
;
62.71
min
;
2235
(bp) ,
744
(aa)
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Product
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(p)ppGpp synthetase I/GTP pyrophosphokinase
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Operon Name
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relA-chpRA-mazG
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Note
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(p)ppGpp synthetase I (GTP pyrophosphokinase); regulation of RNA synthesis; stringent factor; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0015949 - nucleobase, nucleoside and nucleotide interconversion
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Function
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enzyme; Global regulatory functions
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Gene Ontology
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GO:0008152
;
metabolic process ( relA )
GO:0008728
;
GTP diphosphokinase activity ( relA )
GO:0015969
;
guanosine tetraphosphate metabolic process ( relA )
GO:0016301
;
kinase activity ( relA )
GO:0016597
;
amino acid binding ( relA )
GO:0016740
;
transferase activity ( relA )
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PID
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1789147
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EC number
(KEGG Pathway)
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2.7.6.5
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SWISS-PROT
(
Show details
)
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Entry name(Acc.no)
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RELA_ECOLI
(
P11585
)
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- Protein name
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GTP pyrophosphokinase
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- Synonyms
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EC 2.7.6.5, ATP:GTP 3'-pyrophosphotransferase, ppGpp synthetase I) ((p)ppGpp synthetase
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- Gene name
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OrderedLocusNames=b2784, c3347, z4099, ECs3644, SF2797, S2991;
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MMBR References
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J Bacteriol. 1968;95(2):729-31.
Isolation of "relaxed" mutants of Escherichia coli.
Fiil N, Friesen JD.
(
4867755
)
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Cell. Mol. Biol. (Noisy-Le-Grand) 1994;40:899-905.
A role for polyamines in the control of ppGpp levels in Escherichia coli.
Goldemberg, S. H.
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Cell. Mol. Biol. (Noisy-Le-Grand) 1996;42:719-727.
In vitro studies on ppGpp synthetase I from polyamine-starved and unstarved Escherichia coli.
Goldemberg, S. H.
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J Biol Chem. 1989;264:9122-9125
Protein sequences encoded by the relA and the spoT genes of Escherichia coli are interrelated.
Metzger, S., E. Sarubbi, G. Glaser, and M. Cashel.
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J Biol Chem. 1989;264:21146-21152
Characterization of the relA1 mutation and a comparison of relA1 with new relA null alleles in Escherichia coli.
Metzger, S., G. Schreiber, E. Aizenman, M. Cashel, and G. Glaser.
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J Biol Chem. 1988;263:15699-15704
The nucleotide sequence and characterization of the relA gene of Escherichia coli.
Metzger, S., I. B. Dror, E. Aizenman, G. Schreiber, M. Toone, J. D. Friesen, M. Cashel, and G. Glaser.
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J Mol Biol. 1996;259(1):41-57.
Control of spoT-dependent ppGpp synthesis and degradation in Escherichia coli.
Murray KD, Bremer H.
(
8648647
)
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J Bacteriol. 1976;127(3):1587-9.
Method for the isolation of Escherichia coli relaxed mutants, utilizing near-ultraviolet irradiation.
Ramabhadran TV.
(
783151
)
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J Biol Chem. 1991;266:3760-3767
Overexpression of the relA gene in Escherichia coli.
Schreiber, G., S. Metzger, E. Aizenman, S. Roza, M. Cashel, and G. Glaser.
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J Biol Chem. 1993;268:2307-2311
Guanosine tetraphosphate inhibits protein synthesis in vivo. A possible protective mechanism for starvation stress in Escherichia coli.
Svitil, A. L., M. Cashel, and J. W. Zyskind.
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J Biol Chem. 1991;266:5980-5990
Residual guanosine 3',5'-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spoT null mutations.
Xiao, H., M. Kalman, K. Ikehara, S. Zemel, G. Glaser, and M. Cashel.
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Mol Gen Genet. 1979;176(3):343-50.
Involvement of cyclic AMP and its receptor protein in the sensitivity of Escherichia coli K 12 toward serine: excretion of 2-ketobutyrate, a precursor of isoleucine.
Daniel J, Danchin A.
(
230407
)
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| Sequences |
Amino acid
FASTA format
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0001 MVAVRSAHIN KAGEFDPEKW IASLGITSQK SCECLAETWA YCLQQTQGHP DASLLLWRGV EMVEILSTLS
0071 MDIDTLRAAL LFPLADANVV SEDVLRESVG KSVVNLIHGV RDMAAIRQLK ATHTDSVSSE QVDNVRRMLL
0141 AMVDDFRCVV IKLAERIAHL REVKDAPEDE RVLAAKECTN IYAPLANRLG IGQLKWELED YCFRYLHPTE
0211 YKRIAKLLHE RRLDREHYIE EFVGHLRAEM KAEGVKAEVY GRPKHIYSIW RKMQKKNLAF DELFDVRAVR
0281 IVAERLQDCY AALGIVHTHY RHLPDEFDDY VANPKPNGYQ SIHTVVLGPG GKTVEIQIRT KQMHEDAELG
0351 VAAHWKYKEG AAAGGARSGH EDRIAWLRKL IAWQEEMADS GEMLDEVRSQ VFDDRVYVFT PKGDVVDLPA
0421 GSTPLDFAYH IHSDVGHRCI GAKIGGRIVP FTYQLQMGDQ IEIITQKQPN PSRDWLNPNL GYVTTSRGRS
0491 KIHAWFRKQD RDKNILAGRQ ILDDELEHLG ISLKEAEKHL LPRYNFNDVD ELLAAIGGGD IRLNQMVNFL
0561 QSQFNKPSAE EQDAAALKQL QQKSYTPQNR SKDNGRVVVE GVGNLMHHIA RCCQPIPGDE IVGFITQGRG
0631 ISVHRADCEQ LAELRSHAPE RIVDAVWGES YSAGYSLVVR VVANDRSGLL RDITTILANE KVNVLGVASR
0701 SDTKQQLATI DMTIEIYNLQ VLGRVLGKLN QVPDVIDARR LHGS
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 tcccacacac gggacatctg gaatcgatgg
-070 tacttttctc gcgcgttaaa tagttgcgat ttgccgattt cggcaggtct ggtccctaaa ggagaggacg
0001 atggttgcgg taagaagtgc acatatcaat aaggctggtg aatttgatcc ggaaaaatgg atcgcaagtc
0071 tgggtattac cagccagaag tcgtgtgagt gcttagccga aacctgggcg tattgtctgc aacagacgca
0141 ggggcatccg gatgccagtc tgttattgtg gcgtggtgtt gagatggtgg agatcctctc gacattaagt
0211 atggacattg acacgctgcg ggcggcgctg cttttccctc tggcggatgc caacgtagtc agcgaagatg
0281 tgctgcgtga gagcgtcggt aagtcggtcg ttaaccttat tcacggcgtg cgtgatatgg cggcgatccg
0351 ccagctgaaa gcgacgcaca ctgattctgt ttcctccgaa caggtcgata acgttcgccg gatgttattg
0421 gcgatggtcg atgattttcg ctgcgtagtc atcaaactgg cggagcgtat tgctcatctg cgcgaagtaa
0491 aagatgcgcc ggaagatgaa cgtgtactgg cggcaaaaga gtgtaccaac atctacgcac cgctggctaa
0561 ccgtctcgga atcggacaac tgaaatggga actggaagat tactgcttcc gttacctcca tccaaccgaa
0631 tacaaacgaa ttgccaaact gctgcatgaa cggcgtctcg accgcgaaca ctacatcgaa gagttcgttg
0701 gtcatctgcg cgctgagatg aaagctgaag gcgttaaagc ggaagtgtat ggtcgtccga aacacatcta
0771 cagcatctgg cgtaaaatgc agaaaaagaa cctcgccttt gatgagctgt ttgatgtgcg tgcggtacgt
0841 attgtcgccg agcgtttaca ggattgctat gccgcactgg ggatagtgca cactcactat cgccacctgc
0911 cggatgagtt tgacgattac gtcgctaacc cgaaaccaaa cggttatcag tctattcata ccgtggttct
0981 ggggccgggt ggaaaaaccg ttgagatcca aatccgcacc aaacagatgc atgaagatgc agagttgggt
1051 gttgctgcgc actggaaata taaagagggc gcggctgctg gcggcgcacg ttcgggacat gaagaccgga
1121 ttgcctggct gcgtaaactg attgcgtggc aggaagagat ggctgattcc ggcgaaatgc tcgacgaagt
1191 acgtagtcag gtctttgacg accgggtgta cgtctttacg ccgaaaggtg atgtcgttga tttgcctgcg
1261 ggatcaacgc cgctggactt cgcttaccac atccacagtg atgtcggaca ccgctgcatc ggggcaaaaa
1331 ttggcgggcg cattgtgccg ttcacctacc agctgcagat gggcgaccag attgaaatta tcacccagaa
1401 acagccgaac cccagccgtg actggttaaa cccaaacctc ggttacgtca caaccagccg tgggcgttcg
1471 aaaattcacg cctggttccg taaacaggac cgtgacaaaa acattctggc tgggcggcaa atccttgacg
1541 acgagctgga acatctgggg atcagcctga aagaagcaga aaaacatctg ctgccgcgtt acaacttcaa
1611 tgatgtcgac gagttgctgg cggcgattgg tggcggggat atccgtctca atcagatggt gaacttcctg
1681 caatcgcaat ttaataagcc gagtgccgaa gagcaggacg ccgccgcgct gaagcaactt cagcaaaaaa
1751 gctacacgcc gcaaaaccgc agtaaagata acggtcgcgt ggtagtcgaa ggtgttggca acctgatgca
1821 ccacatcgcg cgctgctgcc agccgattcc tggagatgag attgtcggct tcattaccca ggggcgcggt
1891 atttcagtac accgcgccga ttgcgaacaa ctggcggaac tgcgctccca tgcgccagaa cgcattgttg
1961 acgcggtatg gggtgagagc tactccgccg gatattcgct ggtggtccgc gtggtagcta atgatcgtag
2031 tgggttgtta cgtgatatca cgaccattct cgccaacgag aaggtgaacg tgcttggcgt tgccagccgt
2101 agcgacacca aacagcaact ggcgaccatc gacatgacca ttgagattta caacctgcaa gtgctggggc
2171 gcgtgctggg taaactcaac caggtgccgg atgttatcga cgcgcgtcgg ttgcacggga gttaggccga
2241 aatttgctcg tatctacaat gtagattgat atatactgta tctacatatg atagcggttt gaggaaaggg
2311 ttatgatcca cagtagcgta aagcg
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