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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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OCL45-5
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
cysJ
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Related strains
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Strains Search
|
Search strains by gene name:
cysJ
Search strains by all related name:
cysJ b2764 cysP ECK2759 JW2734
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General information (Go to Linear View: )
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Gene Name
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cysJ
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Alternative name
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b2764,cysP,ECK2759,JW2734
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Location, Length
|
2,888,121 - 2,889,920
(
-
)
;
62.25
min
;
1800
(bp) ,
599
(aa)
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Product
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sulfite reductase, alpha subunit, flavoprotein
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Operon Name
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cysJIH
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Note
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sulfite reductase (NADPH), flavoprotein beta subunit; GO_process: GO:0006790 - sulfur metabolic process
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Function
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enzyme; Central intermediary metabolism: Sulfur metabolism
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Gene Ontology
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GO:0000103
;
sulfate assimilation ( cysJ )
GO:0004783
;
sulfite reductase (NADPH) activity ( cysJ )
GO:0005215
;
transporter activity ( cysP )
GO:0005506
;
iron ion binding ( cysJ )
GO:0005515
;
protein binding ( cysJ )
GO:0005515
;
protein binding ( cysP )
GO:0006118
;
electron transport ( cysJ )
GO:0006810
;
transport ( cysJ )
GO:0006810
;
transport ( cysP )
GO:0008271
;
secondary active sulfate transmembrane transporter activity ( cysP )
GO:0008272
;
sulfate transport ( cysP )
GO:0008652
;
amino acid biosynthetic process ( cysJ )
GO:0009055
;
electron carrier activity ( cysJ )
GO:0010181
;
FMN binding ( cysJ )
GO:0015419
;
sulfate transmembrane-transporting ATPase activity ( cysP )
GO:0016491
;
oxidoreductase activity ( cysJ )
GO:0019344
;
cysteine biosynthetic process ( cysJ )
GO:0030288
;
outer membrane-bounded periplasmic space ( cysP )
GO:0042597
;
periplasmic space ( cysP )
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PID
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1789123
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EC number
(KEGG Pathway)
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1.5.1.30
1.5.1.38
1.8.1.2
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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cysJ
(
EG10191
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
5
more]
)
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Entry name(Acc.no)
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CYSP_ECOLI
(
P16700
)
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- Protein name
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Thiosulfate-binding protein precursor.
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- Synonyms
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- Gene name
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Name=cysP; OrderedLocusNames=b2425;
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MMBR References
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FEBS Lett. 1995;374(1):82-4.
NADPH-sulfite reductase flavoprotein from Escherichia coli: contribution to the flavin content and subunit interaction.
Eschenbrenner M, Coves J, Fontecave M.
(
7589518
)
|
J Gen Microbiol. 1987;133(10):2707-17.
Lambda transducing phage and clones carrying genes of the cysJIHDC gene cluster of Escherichia coli K12.
Hunt CL, Colless V, Smith MT, Molasky DO, Malo MS, Loughlin RE.
(
2966849
)
|
Gene. 1987;53(2-3):227-34.
Cloning of the 3'-phosphoadenylyl sulfate reductase and sulfite reductase genes from Escherichia coli K-12.
Li C, Peck HD Jr, Przybyla AE.
(
3301533
)
|
Gene. 1992;122(1):17-25.
Mutagenesis and regulation of the cysJ promoter of Escherichia coli K-12.
Loudon JA, Loughlin RE.
(
1452024
)
|
Biotechnol Appl Biochem. 1990;12(2):212-6.
Molecular cloning of the cys genes (cysC, cysD, cysH, cysI, cysJ, and cysG) responsible for cysteine biosynthesis in Escherichia coli K-12.
Tei H, Murata K, Kimura A.
(
2158792
)
|
J Bacteriol. 1991;173(1):325-33.
High-level expression of Escherichia coli NADPH-sulfite reductase: requirement for a cloned cysG plasmid to overcome limiting siroheme cofactor.
Wu JY, Siegel LM, Kredich NM.
(
1987123
)
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| Sequences |
Amino acid
FASTA format
|
0001 MTTQVPPSAL LPLNPEQLAR LQAATTDLTP TQLAWVSGYF WGVLNQQPAA LAATPAPAAE MPGITIISAS
0071 QTGNARRVAE ALRDDLLAAK LNVKLVNAGD YKFKQIASEK LLIVVTSTQG EGEPPEEAVA LHKFLFSKKA
0141 PKLENTAFAV FSLGDSSYEF FCQSGKDFDS KLAELGGERL LDRVDADVEY QAAASEWRAR VVDALKSRAP
0211 VAAPSQSVAT GAVNEIHTSP YSKDAPLVAS LSVNQKITGR NSEKDVRHIE IDLGDSGMRY QPGDALGVWY
0281 QNDPALVKEL VELLWLKGDE PVTVEGKTLP LNEALQWHFE LTVNTANIVE NYATLTRSET LLPLVGDKAK
0351 LQHYAATTPI VDMVRFSPAQ LDAEALINLL RPLTPRLYSI ASSQAEVENE VHVTVGVVRY DVEGRARAGG
0421 ASSFLADRVE EEGEVRVFIE HNDNFRLPAN PETPVIMIGP GTGIAPFRAF MQQRAADEAP GKNWLFFGNP
0491 HFTEDFLYQV EWQRYVKDGV LTRIDLAWSR DQKEKVYVQD KLREQGAELW RWINDGAHIY VCGDANRMAK
0561 DVEQALLEVI AEFGGMDTEA ADEFLSELRV ERRYQRDVY
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ttaatccaca ccgtttgccc cgttaacctt
-070 accttctctt ctgttttatg ggcgctgaca gggcgcagaa acagctttgc ttactggaac ataacgacgc
0001 atgacgacac aggtcccacc ttccgcgttg cttccgttga acccggagca actggcacgc cttcaggcgg
0071 ccacgaccga tttaactccc acccagcttg cctgggtttc tggctatttc tggggcgtac tcaatcagca
0141 gcctgctgcg cttgcagcga cgccagcgcc agccgcagaa atgccgggta taactattat ctccgcctcg
0211 caaaccggca atgcgcgccg ggttgctgaa gcattacgtg atgatttatt agcagcaaaa ctgaacgtta
0281 agctggtgaa cgcgggcgac tataaattca aacaaatcgc cagcgaaaaa ctgctcatcg tagtgacgtc
0351 aacgcaaggg gaaggggaac cgccggaaga agccgtcgcg ctgcataagt tcctgttctc caaaaaagcg
0421 ccaaagctgg aaaacaccgc gtttgccgtg tttagcctcg gcgatagctc ttatgaattt ttctgccagt
0491 ccgggaaaga tttcgacagc aagctggcgg aactgggtgg tgaacgcctg ctcgaccgtg tcgatgccga
0561 tgttgaatac caggctgctg ccagcgagtg gcgcgcccgc gtggttgatg cgcttaaatc gcgtgcgcct
0631 gtcgcggcac cttcgcaatc cgtcgctact ggcgcggtaa atgaaatcca caccagcccg tacagcaaag
0701 acgcgccgct ggtggctagc ctctctgtta accagaaaat taccgggcgt aactctgaaa aagacgttcg
0771 ccatatcgaa attgacttag gtgactcggg catgcgttac cagccgggtg acgcgctggg cgtctggtat
0841 cagaacgatc cggcactggt gaaagaactt gtcgaactgc tgtggctgaa aggcgatgaa cctgtcaccg
0911 tcgagggcaa aacgttgcct ctgaacgaag cgctacagtg gcacttcgaa ctgaccgtca acaccgccaa
0981 cattgttgag aattacgcca cgcttacccg cagtgaaaca ctgctgccgc tggtgggcga taaagcgaag
1051 ttacagcatt acgccgcgac gacgccgatt gttgacatgg tgcgtttctc cccggcacag cttgatgccg
1121 aagcgctaat taatctgctg cgcccgctga cgccgcgtct gtattccatc gcctcctcgc aggcggaagt
1191 cgagaacgaa gtacacgtca ccgttggtgt ggtgcgttac gacgtggaag gccgcgcccg tgccggtggt
1261 gcctccagct tcctcgctga ccgcgtggaa gaagagggcg aagtccgcgt atttatcgaa cataacgata
1331 acttccgcct gccagccaat ccagaaaccc cggtgattat gattggccca ggcaccggta ttgcgccgtt
1401 ccgcgccttt atgcagcaac gcgccgccga cgaagcgcca ggtaaaaact ggctgttctt tggtaatccg
1471 cactttacgg aagacttcct gtaccaggtg gagtggcagc gctacgtcaa agatggcgtg ctgacacgta
1541 tcgatcttgc ctggtcgcgc gatcaaaaag aaaaagttta cgtacaagac aaactgcgcg aacagggcgc
1611 ggagctgtgg cgctggatca atgatggtgc ccacatttat gtctgcggcg acgctaatcg catggcgaaa
1681 gacgttgagc aggcacttct ggaagtgatt gccgaatttg gtggcatgga caccgaagcg gcggatgaat
1751 ttttaagtga gctgcgcgta gagcgccgtt atcagcgaga tgtctactaa tgagcgaaaa acatccaggg
1821 cctttagtgg tcgaaggaaa actgacagac gccgagcgca tgaagcatga aagcaactac ctgcgcggca
1891 ccattgcgga
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