|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
|
|
Deletion
|
DE(cysH-cysJ)264
,
OCL45-5
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
cysI
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
cysI
Search strains by all related name:
cysI b2763 cysQ ECK2758 JW2733
|
General information (Go to Linear View: )
|
|
Gene Name
|
cysI
|
|
Alternative name
|
b2763,cysQ,ECK2758,JW2733
|
|
Location, Length
|
2,886,409 - 2,888,121
(
-
)
;
62.21
min
;
1713
(bp) ,
570
(aa)
|
|
Product
|
sulfite reductase, beta subunit, NAD(P)-binding, heme-binding
|
|
Operon Name
|
cysJIH
|
|
Note
|
sulfite reductase, alpha subunit; GO_process: GO:0006790 - sulfur metabolic process
|
|
Function
|
enzyme; Central intermediary metabolism: Sulfur metabolism
|
|
Gene Ontology
|
GO:0000103
;
sulfate assimilation ( cysI )
GO:0000287
;
magnesium ion binding ( cysQ )
GO:0004437
;
inositol or phosphatidylinositol phosphatase activity ( cysQ )
GO:0004783
;
sulfite reductase (NADPH) activity ( cysI )
GO:0005506
;
iron ion binding ( cysI )
GO:0005886
;
plasma membrane ( cysQ )
GO:0006118
;
electron transport ( cysI )
GO:0008652
;
amino acid biosynthetic process ( cysI )
GO:0009337
;
sulfite reductase complex (NADPH) ( cysI )
GO:0016020
;
membrane ( cysQ )
GO:0016491
;
oxidoreductase activity ( cysI )
GO:0019344
;
cysteine biosynthetic process ( cysI )
GO:0020037
;
heme binding ( cysI )
GO:0046872
;
metal ion binding ( cysI )
GO:0046872
;
metal ion binding ( cysQ )
GO:0050661
;
NADP binding ( cysI )
GO:0051536
;
iron-sulfur cluster binding ( cysI )
GO:0051539
;
4 iron, 4 sulfur cluster binding ( cysI )
|
|
PID
|
1789122
|
EC number
(KEGG Pathway)
|
1.8.1.2
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
cysI
(
EG10190
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show details
[
5
more]
)
|
Entry name(Acc.no)
|
CYSI_ECOLI
(
P17846
)
|
|
- Protein name
|
Sulfite reductase [NADPH] hemoprotein beta-component
|
|
- Synonyms
|
EC 1.8.1.2, SIR-HP, SIRHP
|
|
- Gene name
|
Name=cysI; OrderedLocusNames=b2763;
|
|
MMBR References
|
FEBS Lett. 1995;374(1):82-4.
NADPH-sulfite reductase flavoprotein from Escherichia coli: contribution to the flavin content and subunit interaction.
Eschenbrenner M, Coves J, Fontecave M.
(
7589518
)
|
J Gen Microbiol. 1987;133(10):2707-17.
Lambda transducing phage and clones carrying genes of the cysJIHDC gene cluster of Escherichia coli K12.
Hunt CL, Colless V, Smith MT, Molasky DO, Malo MS, Loughlin RE.
(
2966849
)
|
Gene. 1987;53(2-3):227-34.
Cloning of the 3'-phosphoadenylyl sulfate reductase and sulfite reductase genes from Escherichia coli K-12.
Li C, Peck HD Jr, Przybyla AE.
(
3301533
)
|
Biotechnol Appl Biochem. 1990;12(2):212-6.
Molecular cloning of the cys genes (cysC, cysD, cysH, cysI, cysJ, and cysG) responsible for cysteine biosynthesis in Escherichia coli K-12.
Tei H, Murata K, Kimura A.
(
2158792
)
|
J Bacteriol. 1991;173(1):325-33.
High-level expression of Escherichia coli NADPH-sulfite reductase: requirement for a cloned cysG plasmid to overcome limiting siroheme cofactor.
Wu JY, Siegel LM, Kredich NM.
(
1987123
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSEKHPGPLV VEGKLTDAER MKHESNYLRG TIAEDLNDGL TGGFKGDNFL LIRFHGMYQQ DDRDIRAERA
0071 EQKLEPRHAM LLRCRLPGGV ITTKQWQAID KFAGENTIYG SIRLTNRQTF QFHGILKKNV KPVHQMLHSV
0141 GLDALATAND MNRNVLCTSN PYESQLHAEA YEWAKKISEH LLPRTRAYAE IWLDQEKVAT TDEEPILGQT
0211 YLPRKFKTTV VIPPQNDIDL HANDMNFVAI AENGKLVGFN LLVGGGLSIE HGNKKTYART ASEFGYLPLE
0281 HTLAVAEAVV TTQRDWGNRT DRKNAKTKYT LERVGVETFK AEVERRAGIK FEPIRPYEFT GRGDRIGWVK
0351 GIDDNWHLTL FIENGRILDY PARPLKTGLL EIAKIHKGDF RITANQNLII AGVPESEKAK IEKIAKESGL
0421 MNAVTPQREN SMACVSFPTC PLAMAEAERF LPSFIDNIDN LMAKHGVSDE HIVMRVTGCP NGCGRAMLAE
0491 VGLVGKAPGR YNLHLGGNRI GTRIPRMYKE NITEPEILAS LDELIGRWAK EREAGEGFGD FTVRAGIIRP
0561 VLDPARDLWD
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 tggaagtgat tgccgaattt ggtggcatgg
-070 acaccgaagc ggcggatgaa tttttaagtg agctgcgcgt agagcgccgt tatcagcgag atgtctacta
0001 atgagcgaaa aacatccagg gcctttagtg gtcgaaggaa aactgacaga cgccgagcgc atgaagcatg
0071 aaagcaacta cctgcgcggc accattgcgg aagatttaaa cgacggtctg accggcggct ttaagggcga
0141 caacttcctg ctgattcgct tccacggcat gtatcagcag gatgaccgcg acatccgcgc cgaacgtgct
0211 gaacagaagc tggagccgcg ccacgcgatg ctgcttcgct gtcgtctgcc gggtggggtg attaccacta
0281 aacagtggca ggcgatcgac aaatttgccg gtgaaaacac catctatggc agcattcgcc tgaccaaccg
0351 ccagacgttt cagttccacg gcattctgaa aaagaacgtc aaaccggtgc accagatgct gcactcggtc
0421 ggtcttgatg cgctggcgac agctaacgac atgaaccgta acgtactctg cacctcgaac ccttacgagt
0491 cgcagctgca cgcggaagcg tacgagtggg cgaagaagat ttctgagcat ctgttgcctc gtacccgcgc
0561 gtatgcggag atctggctcg accaggaaaa agtcgccact actgatgaag aaccgatcct cggccagacc
0631 tacctgccgc gtaaattcaa aaccacggta gtgatcccgc cacagaacga tatcgatctg cacgccaacg
0701 acatgaactt cgtggcgatc gccgaaaacg gcaagctggt gggctttaac ctgttggtgg gcggtgggct
0771 ttccatcgaa cacggcaaca agaaaaccta cgcccgcacc gcgagtgagt ttggctatct gccgctggag
0841 catacgctgg cggtggccga agccgtcgtg acaactcagc gtgactgggg taaccgaacc gatcgtaaaa
0911 atgccaaaac caaatacacg ctggagcgcg tgggggttga gacgtttaaa gcggaagtgg agcgtcgcgc
0981 ggggatcaaa tttgaaccga tccgtccata tgagttcacc ggacgaggcg atcgtattgg ctgggttaag
1051 ggcattgatg ataactggca cctgacgctg tttatcgaaa atggtcgcat ccttgattat ccggcgcgtc
1121 cgctgaaaac cggcctgctg gagatcgcga agatccacaa aggcgatttc cgcattacgg cgaaccagaa
1191 tctgatcatc gccggtgtac cggaaagcga gaaagcgaag atcgagaaga tcgccaaaga gagcgggtta
1261 atgaatgccg tcacgccgca gcgtgaaaac tcgatggctt gcgtgtcatt cccgacttgc ccgctggcga
1331 tggcggaagc agagcgtttc ctgccgtctt ttatcgacaa catcgataat ttaatggcga aacatggtgt
1401 cagcgatgag catatcgtga tgcgtgtaac aggctgcccg aacggttgtg gtcgcgcgat gctggcggaa
1471 gtgggcctgg tgggtaaagc gccgggtcgc tacaacctgc atcttggcgg caaccgcatt gggacacgta
1541 tcccacggat gtataaagaa aacatcaccg agccggaaat cctggcgtcg cttgatgaac tgatagggcg
1611 ctgggcgaaa gagcgcgaag cgggtgaagg cttcggcgac tttacggtgc gtgcgggcat cattcgcccg
1681 gtgctcgatc cggcgcgtga tttgtgggat taaccatcag cccggtcttg taggcctgat aagaacgcgt
1751 gagcgtcgca tcaggcaagg caaacagtga ggaatctatg tccaaactcg atctaaacgc cct
|
