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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
|
|
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Deletion
|
OCL45-5
(D)
|
|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
cysH
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Related strains
|
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Strains Search
|
Search strains by gene name:
cysH
Search strains by all related name:
cysH b2762 ECK2757 JW2732
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General information (Go to Linear View: )
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Gene Name
|
cysH
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Alternative name
|
b2762,ECK2757,JW2732
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|
Location, Length
|
2,885,600 - 2,886,334
(
-
)
;
62.19
min
;
735
(bp) ,
244
(aa)
|
|
Product
|
3'-phosphoadenosine 5'-phosphosulfate reductase
|
|
Operon Name
|
cysJIH
|
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Note
|
GO_process: GO:0006790 - sulfur metabolic process
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Function
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enzyme; Central intermediary metabolism: Sulfur metabolism
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Gene Ontology
|
GO:0004604
;
phosphoadenylyl-sulfate reductase (thioredoxin) activity ( cysH )
GO:0005515
;
protein binding ( cysH )
GO:0005737
;
cytoplasm ( cysH )
GO:0008152
;
metabolic process ( cysH )
GO:0016491
;
oxidoreductase activity ( cysH )
GO:0016740
;
transferase activity ( cysH )
GO:0019344
;
cysteine biosynthetic process ( cysH )
GO:0019379
;
sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) ( cysH )
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|
PID
|
1789121
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EC number
(KEGG Pathway)
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1.8.4.8
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
|
cysH
(
EG10189
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
|
CYSH_ECOLI
(
P17854
)
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- Protein name
|
Phosphoadenosine phosphosulfate reductase
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- Synonyms
|
EC 1.8.4.8, PAPS reductase, thioredoxin dependent, PAdoPS reductase, 3'- phosphoadenylylsulfate reductase, PAPS sulfotransferase
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- Gene name
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Name=cysH; OrderedLocusNames=b2762;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
|
cysH
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
|
Chain , Phospho-Adenylyl-Sulfate Reductase
|
|
SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Phosphoadenosine phosphosulfate reductase
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PSI-BLAST
|
GMP synthase [glutamine-hydrolyzing]
|
|
nr
(database updated :
2007.02.20
)
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BLAST
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phosphoadenosine phosphosulfate reductase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
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MMBR References
|
J Gen Microbiol. 1987;133(10):2707-17.
Lambda transducing phage and clones carrying genes of the cysJIHDC gene cluster of Escherichia coli K12.
Hunt CL, Colless V, Smith MT, Molasky DO, Malo MS, Loughlin RE.
(
2966849
)
|
FEBS Lett. 1990;260(1):6-9.
PAPS-reductase of Escherichia coli. Correlating the N-terminal amino acid sequence with the DNA of gene cys H.
Krone FA, Westphal G, Meyer HE, Schwenn JD.
(
2404794
)
|
Mol Gen Genet. 1991;225(2):314-9.
Characterisation of the gene cysH and of its product phospho-adenylylsulphate reductase from Escherichia coli.
Krone FA, Westphal G, Schwenn JD.
(
2005873
)
|
Gene. 1987;53(2-3):227-34.
Cloning of the 3'-phosphoadenylyl sulfate reductase and sulfite reductase genes from Escherichia coli K-12.
Li C, Peck HD Jr, Przybyla AE.
(
3301533
)
|
Biotechnol Appl Biochem. 1990;12(2):212-6.
Molecular cloning of the cys genes (cysC, cysD, cysH, cysI, cysJ, and cysG) responsible for cysteine biosynthesis in Escherichia coli K-12.
Tei H, Murata K, Kimura A.
(
2158792
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSKLDLNALN ELPKVDRILA LAETNAELEK LDAEGRVAWA LDNLPGEYVL SSSFGIQAAV SLHLVNQIRP
0071 DIPVILTDTG YLFPETYRFI DELTDKLKLN LKVYRATESA AWQEARYGKL WEQGVEGIEK YNDINKVEPM
0141 NRALKELNAQ TWFAGLRREQ SGSRANLPVL AIQRGVFKVL PIIDWDNRTI YQYLQKHGLK YHPLWDEGYL
0211 SVGDTHTTRK WEPGMAEEET RFFGLKRECG LHEG
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 atccggcgcg tgatttgtgg gattaaccat
-070 cagcccggtc ttgtaggcct gataagaacg cgtgagcgtc gcatcaggca aggcaaacag tgaggaatct
0001 atgtccaaac tcgatctaaa cgccctgaac gaactgccga aggtagatcg cattctggcg ctggcggaaa
0071 ctaacgccga actggaaaaa ctggacgctg aaggccgcgt agcctgggcg ctggataatc tgcccggtga
0141 atatgtactt tcttccagct ttggcattca ggcggcggtg agcctgcatc tggtgaatca aattcgcccg
0211 gatattccgg tgatcctcac cgatacgggt tacttgttcc cggaaaccta ccgctttatt gacgagttaa
0281 cggacaaact caagctcaac ctgaaagtgt accgtgctac cgaaagcgca gcctggcagg aagcacgcta
0351 cggaaaactg tgggagcagg gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt cgaaccgatg
0421 aaccgggctc tgaaagaact gaatgcgcaa acctggtttg ctggcctgcg ccgtgaacaa tccggcagtc
0491 gtgccaattt accggtgctg gcaattcagc gtggcgtatt taaagtgctg ccgattatcg actgggataa
0561 ccgaactatt tatcagtacc tgcaaaaaca tggcctgaaa tatcacccat tatgggatga aggatattta
0631 tcggtgggcg atacccatac aacccgtaaa tgggaacccg gcatggcgga agaagaaacg cgtttctttg
0701 gcttaaaaag ggaatgtggg ttacacgaag ggtaaatatt gccggacaaa ttaaacgcct ctgtcagaaa
0771 tgatgggggc gtttttattt tgcgagcagc attcctgatt ttaaacgtga acgccgcggg aagaa
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