MG1655
W3110
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Gene Report : cysD
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCL46 (D)  ,  OCL46-2 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  cysD
Related strains
     Strains Search Search strains by gene name:  cysD   Search strains by all related name:  cysD b2752 ECK2747 JW2722

General information  (Go to Linear View:)
 Gene Name cysD  
 Alternative name b2752,ECK2747,JW2722  
 Location, Length 2,873,443 - 2,874,351 (  -  ) ;   61.93 min ; 909 (bp) ,   302 (aa) Go to Linear View
 Product sulfate adenylyltransferase, subunit 2  
 Operon Name cysDNC  
 Note ATP:sulfurylase (ATP:sulfate adenylyltransferase), subunit 2; GO_process: GO:0006790 - sulfur metabolic process  
 Function enzyme; Central intermediary metabolism: Sulfur metabolism  
 Gene Ontology GO:0000103 ; sulfate assimilation ( cysD )
GO:0004781 ; sulfate adenylyltransferase (ATP) activity ( cysD )
GO:0008152 ; metabolic process ( cysD )
GO:0016740 ; transferase activity ( cysD )
GO:0016779 ; nucleotidyltransferase activity ( cysD )
GO:0019421 ; sulfate reduction, APS pathway ( cysD )
 PID 1789109  
 EC number
  (KEGG Pathway)
 
2.7.7.4  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene cysD ( EG10186 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) CYSD_ECOLI ( P21156 )
    -  Protein name Sulfate adenylyltransferase subunit 2  
    -  Synonyms EC 2.7.7.4, Sulfate adenylate transferase, SAT, ATP-sulfurylase small subunit  
    -  Gene name Name=cysD; OrderedLocusNames=b2752;  

 Linear View (Whole Mode)
View Location
   2865.0  –  2890.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP cysD (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of A Gtp-Regulated Atp Sulfurylase Heterodimer From Pseudomonas Syringae  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Sulfate adenylyltransferase subunit 2  
         PSI-BLAST Sulfate adenylyltransferase subunit 2
    nr     (database updated : 2007.02.20 )
         BLAST sulfate adenylyltransferase subunit 2 [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    MITOCH_CARRIER     

Other Cross-Reference
    COG COG0175EH 
    EcoCyc cysD 

MMBR References
J Gen Microbiol. 1987;133(10):2707-17.
Lambda transducing phage and clones carrying genes of the cysJIHDC gene cluster of Escherichia coli K12.
Hunt CL, Colless V, Smith MT, Molasky DO, Malo MS, Loughlin RE. ( 2966849 )
Gene. 1990;87(1):127-31.
Promoter elements and regulation of expression of the cysD gene of Escherichia coli K-12.
Malo MS, Loughlin RE. ( 2185135 )
Biotechnol Appl Biochem. 1990;12(2):212-6.
Molecular cloning of the cys genes (cysC, cysD, cysH, cysI, cysJ, and cysG) responsible for cysteine biosynthesis in Escherichia coli K-12.
Tei H, Murata K, Kimura A. ( 2158792 )

Sequences
Amino acid
FASTA format
0001 MDQIRLTHLR QLEAESIHII REVAAEFSNP VMLYSIGKDS SVMLHLARKA FYPGTLPFPL LHVDTGWKFR 
0071 EMYEFRDRTA KAYGCELLVH KNPEGVAMGI NPFVHGSAKH TDIMKTEGLK QALNKYGFDA AFGGARRDEE 
0141 KSRAKERIYS FRDRFHRWDP KNQRPELWHN YNGQINKGES IRVFPLSNWT EQDIWQYIWL ENIDIVPLYL 
0211 AAERPVLERD GMLMMIDDNR IDLQPGEVIK KRMVRFRTLG CWPLTGAVES NAQTLPEIIE EMLVSTTSER 
0281 QGRVIDRDQA GSMELKKRQG YF

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             gaactactca ttctaattgg taatttcatt 
-070 cgttctctta cgctccctat agtcgaaaca tctgatggca agaaaatagc ggtattgcaa aggaacggtt 
0001 atggatcaaa tacgacttac tcacctgcgg caactggagg cggaaagcat ccacattatt cgcgaggtgg 
0071 cggcagaatt ctcaaatccg gtgatgctct actctatcgg taaagattcc agcgtcatgc tgcatctggc 
0141 gcgcaaggcg ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc 
0211 gagatgtatg agttccgcga tcgtactgct aaagcctacg gctgcgaact gctggtgcat aaaaacccgg 
0281 aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat accgatatta tgaaaactga 
0351 aggcctgaaa caggcgctga acaaatacgg ttttgatgcc gccttcggtg gtgcgcgccg tgacgaagag 
0421 aaatcccgcg ctaaagagcg aatttactct ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc 
0491 gcccggagct gtggcacaac tacaacgggc aaattaacaa aggcgaaagc atccgcgtct tcccgctctc 
0561 taactggacc gagcaggata tctggcaata catctggctg gaaaatatcg acattgttcc gctatatctc 
0631 gctgcggaac gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt atcgacctgc 
0701 aaccgggcga agtgattaaa aaacggatgg tgcgtttccg tacgctgggc tgctggccgc tgaccggtgc 
0771 ggtggagtca aatgcacaaa cactgccgga aatcatcgaa gagatgctgg tttccaccac cagtgaacgt 
0841 cagggccgcg tgattgaccg cgaccaggcg gggtctatgg agctgaaaaa acgtcagggg tatttttaag 
0911 atgaacaccg cacttgcaca acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac 
0981 ataaaagcct gctgcgtttt ctgacctgt