|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Mol Gen Genet. 1995;248(2):190-4.
Sigma S-dependent overexpression of ftsZ in an Escherichia coli K-12 rpoB mutant that is resistant to the division inhibitors DicB and DicF RNA.
Cam K, Cuzange A, Bouche JP.
(
7651342
)
|
|
|
Deletion
|
OCL46
(D)
,
OCL46-1
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
rpoS
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
rpoS
Search strains by all related name:
rpoS abrD appR b2741 csi2 dpeB ECK2736 JW5437 katF nur otsX sigS
|
General information (Go to Linear View: )
|
|
Gene Name
|
rpoS
|
|
Alternative name
|
abrD,appR,b2741,csi2,dpeB,ECK2736,JW5437,katF,nur,otsX,sigS
|
|
Location, Length
|
2,864,581 - 2,865,573
(
-
)
;
61.74
min
;
993
(bp) ,
330
(aa)
|
|
Product
|
RNA polymerase, sigma S (sigma 38) factor
|
|
Operon Name
|
nlpD-rpoS
|
|
Note
|
RNA polymerase, sigma S (sigma38) factor; synthesis of many growth phase related proteins; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006350 - transcription; GO_process: GO:0006970 - response to osmotic stress
|
|
Function
|
enzyme; Global regulatory functions
|
|
Gene Ontology
|
GO:0003677
;
DNA binding ( rpoS )
GO:0003700
;
transcription factor activity ( rpoS )
GO:0006350
;
transcription ( rpoS )
GO:0006352
;
transcription initiation ( rpoS )
GO:0006355
;
regulation of transcription, DNA-dependent ( rpoS )
GO:0016987
;
sigma factor activity ( rpoS )
|
|
PID
|
1789098
|
EC number
(KEGG Pathway)
|
|
|
SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
RPOS_ECOLI
(
P13445
;
Q79EB7
)
|
|
- Protein name
|
RNA polymerase sigma factor rpoS
|
|
- Synonyms
|
Sigma-38
|
|
- Gene name
|
OrderedLocusNames=b2741;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
rpoS
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain F, Crystal Structure Of The Rna Polymerase Holoenzyme From Thermus Thermophilus At 2.6a Resolution
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
RNA polymerase sigma factor rpoS
|
|
PSI-BLAST
|
RNA polymerase sigma factor rpoD
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
RNA polymerase sigma factor [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
RGD
SIGMA70_1
SIGMA70_2
|
|
MMBR References
|
J Bacteriol. 1998;180:1030-1036
Importance of RpoS and Dps in survival of exposure of both exponential- and stationary-phase Escherichia coli cells to the electrophile N-ethylmaleimide.
Ferguson, G. P., R. I. Creighton, Y. Nikolaev, and I. R. Booth.
|
J Bacteriol. 1993;175:7982-7989
Synthesis of the stationary-phase sigma factor s is positively regulated by ppGpp.
Gentry, D. R., V. J. Hernandez, L. H. Nguyen, D. B. Jensen, and M. Cashel.
|
J Bacteriol. 1993;175(9):2483-9.
Protein-protein communication within the transcription apparatus.
Ishihama A.
(
8478317
)
|
J Bacteriol. 1998;180:1095-1102
Multiple regions on the Escherichia coli heat shock transcription factor sigma32 determine core RNA polymerase binding specificity.
Joo, D. M., A. Nolte, R. Calendar, Y. N. Zhou, and D. J. Jin.
|
J Bacteriol. 1992;174:889-898
Molecular cloning and physical mapping of the otsBA genes, which encode the osmoregulatory trehalose pathway of Escherichia coli: evidence that transcription is activated by katF (AppR).
Kaasen, I., P. Falkenberg, O. B. Styrvold, and A. R. Strom.
|
J Bacteriol. 1995;177:4676-4680
Identification of transcriptional start sites and the role of ppGpp in the expression of rpoS, the structural gene for the sigma S subunit of RNA polymerase in Escherichia coli.
Lange, R., D. Fischer, and R. Hengge-Aronis.
|
Mol Microbiol. 1991;5(1):49-59.
Identification of a central regulator of stationary-phase gene expression in Escherichia coli.
Lange R, Hengge-Aronis R.
(
1849609
)
|
J Bacteriol. 1984;160(2):668-75.
Genetic mapping of katF, a locus that with katE affects the synthesis of a second catalase species in Escherichia coli.
Loewen PC, Triggs BL.
(
6094482
)
|
J Bacteriol. 1994;176:5378-5384
Evidence for involvement of proteins HU and RpoS in transcription of the osmoresponsive proU operon in Escherichia coli.
Manna, D., and J. Gowrishankar.
|
Gene. 1988;73(2):337-45.
Cloning and physical characterization of katE and katF required for catalase HPII expression in Escherichia coli.
Mulvey MR, Sorby PA, Triggs-Raine BL, Loewen PC.
(
2977357
)
|
Nucleic Acids Res. 1989;17(23):9979-91.
Nucleotide sequence of katF of Escherichia coli suggests KatF protein is a novel sigma transcription factor.
Mulvey MR, Loewen PC.
(
2690013
)
|
J Bacteriol. 1998;180:846-854
Roles of DnaK and RpoS in starvation-induced thermotolerance of Escherichia coli.
Rockabrand, D., K. Livers, T. Austin, R. Kaiser, D. Jensen, R. Burgess, and P. Blum.
|
Proc Natl Acad Sci U S A. 1989;86:3271-3275
Exonuclease III and the catalase hydroperoxidase II in Escherichia coli are both regulated by the katF gene product.
Sak, B. D., A. Eisenstark, and D. Touati.
|
J Bacteriol. 1986;168(1):13-21.
Control of sensitivity to inactivation by H2O2 and broad-spectrum near-UV radiation by the Escherichia coli katF locus.
Sammartano LJ, Tuveson RW, Davenport R.
(
3019996
)
|
J Bacteriol. 1992;174:4769-4776
Regulation of katF and katE in Escherichia coli K-12 by weak acids.
Schellhorn, H. E., and V. L. Stones.
|
Res Microbiol. 1991;142(1):29-36.
Are appR and katF the same Escherichia coli gene encoding a new sigma transcription initiation factor?
Touati E, Dassa E, Dassa J, Boquet PL, Touati D.
(
1648776
)
|
Mol Gen Genet. 1986;202(2):257-64.
Pleiotropic mutations in appR reduce pH 2.5 acid phosphatase expression and restore succinate utilisation in CRP-deficient strains of Escherichia coli.
Touati E, Dassa E, Boquet PL.
(
3517593
)
|
J Bacteriol. 1997;179:7476-7487
Negative regulation of mutS and mutH repair gene expression by the Hfq and RpoS global regulators of Escherichia coli K-12.
Tsui, H. C., G. Feng, and M. E. Winkler.
|
J Bacteriol. 1998;180:2623-2629
The L-isoaspartyl protein repair methyltransferase enhances survival of aging Escherichia coli subjected to secondary environmental stresses.
Visick, J., H. Cai, and S. Clarke.
|
J Bacteriol. 1994;176:7638-7645
Induction of the Escherichia coli aidB gene under oxygen-limiting conditions requires a functional rpoS (katF) gene.
Volkert, M. R., L. I. Hajec, Z. Matijasevic, F. C. Fang, and R. Prince.
|
| Sequences |
Amino acid
FASTA format
|
0001 MSQNTLKVHD LNEDAEFDEN GVEVFDEKAL VEQEPSDNDL AEEELLSQGA TQRVLDATQL YLGEIGYSPL
0071 LTAEEEVYFA RRALRGDVAS RRRMIESNLR LVVKIARRYG NRGLALLDLI EEGNLGLIRA VEKFDPERGF
0141 RFSTYATWWI RQTIERAIMN QTRTIRLPIH IVKELNVYLR TARELSHKLD HEPSAEEIAE QLDKPVDDVS
0211 RMLRLNERIT SVDTPLGGDS EKALLDILAD EKENGPEDTT QDDDMKQSIV KWLFELNAKQ REVLARRFGL
0281 LGYEAATLED VGREIGLTRE RVRQIQVEGL RRLREILQTQ GLNIEALFRE
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 aatccgtaaa cccgctgcgt tatttgccgc
-070 agcgataaat cggcggaacc aggcttttgc ttgaatgttc cgtcaaggga tcacgggtag gagccacctt
0001 atgagtcaga atacgctgaa agttcatgat ttaaatgaag atgcggaatt tgatgagaac ggagttgagg
0071 tttttgacga aaaggcctta gtagaacagg aacccagtga taacgatttg gccgaagagg aactgttatc
0141 gcagggagcc acacagcgtg tgttggacgc gactcagctt taccttggtg agattggtta ttcaccactg
0211 ttaacggccg aagaagaagt ttattttgcg cgtcgcgcac tgcgtggaga tgtcgcctct cgccgccgga
0281 tgatcgagag taacttgcgt ctggtggtaa aaattgcccg ccgttatggc aatcgtggtc tggcgttgct
0351 ggaccttatc gaagagggca acctggggct gatccgcgcg gtagagaagt ttgacccgga acgtggtttc
0421 cgcttctcaa catacgcaac ctggtggatt cgccagacga ttgaacgggc gattatgaac caaacccgta
0491 ctattcgttt gccgattcac atcgtaaagg agctgaacgt ttacctgcga accgcacgtg agttgtccca
0561 taagctggac catgaaccaa gtgcggaaga gatcgcagag caactggata agccagttga tgacgtcagc
0631 cgtatgcttc gtcttaacga gcgcattacc tcggtagaca ccccgctggg tggtgattcc gaaaaagcgt
0701 tgctggacat cctggccgat gaaaaagaga acggtccgga agataccacg caagatgacg atatgaagca
0771 gagcatcgtc aaatggctgt tcgagctgaa cgccaaacag cgtgaagtgc tggcacgtcg attcggtttg
0841 ctggggtacg aagcggcaac actggaagat gtaggtcgtg aaattggcct cacccgtgaa cgtgttcgcc
0911 agattcaggt tgaaggcctg cgccgtttgc gcgaaatcct gcaaacgcag gggctgaata tcgaagcgct
0981 gttccgcgag taagtaagca tctgtcagaa aggccagtct caagcgaggc tggccttttc tgtgcacaat
1051 aaaaggtccg atgcccatcg gaccttttta ttaaggtcaa att
|
