MG1655
W3110
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Gene Report : srlB
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion 21 (LD)  ,  DE(recA-srlR)300  ,  OCL47,48-1 (LD 21_CRS) (D)  ,  OCL47,48-2 (LD 21_P/O) (D)  ,  OCL48 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  srlB
Related strains
     Strains Search Search strains by gene name:  srlB   Search strains by all related name:  srlB b2704 ECK2699 gutB JW2673

General information  (Go to Linear View:)
 Gene Name srlB  
 Alternative name b2704,ECK2699,gutB,JW2673  
 Location, Length 2,825,384 - 2,825,755 (  +  ) ;   60.90 min ; 372 (bp) ,   123 (aa) Go to Linear View
 Product glucitol/sorbitol-specific enzyme IIA component of PTS  
 Operon Name srlAEBD-gutM-srlR-gutQ  
 Note PTS system, glucitol/sorbitol-specific enzyme IIA component; GO_process: GO:0016052 - carbohydrate catabolic process  
 Function enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols  
 Gene Ontology GO:0005351 ; sugar:hydrogen ion symporter activity ( srlB )
GO:0005737 ; cytoplasm ( srlB )
GO:0006810 ; transport ( srlB )
GO:0008643 ; carbohydrate transport ( srlB )
GO:0008982 ; protein-N(PI)-phosphohistidine-sugar phosphotransferase activity ( srlB )
GO:0009401 ; phosphoenolpyruvate-dependent sugar phosphotransferase system ( srlB )
GO:0016301 ; kinase activity ( srlB )
GO:0016740 ; transferase activity ( srlB )
 PID 1789056  
 EC number
  (KEGG Pathway)
 
2.7.1.69  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) PTHA_ECOLI ( P05706 ; P77029; Q47243; Q47244; Q57043; Q57084; Q57246 )
    -  Protein name PTS system, glucitol/sorbitol-specific IIA component  
    -  Synonyms EIIA-Gut, Glucitol/sorbitol-permease IIA component, Phosphotransferase enzyme II, A component, EC 2.7.1.69, EIII-Gut  
    -  Gene name Name=srlB; Synonyms=gutB; OrderedLocusNames=b2704;  

 Linear View (Whole Mode)
View Location
   2815.0  –  2840.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP srlB (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, The Crystal Structure Of Pts System Iia Component From Enterococcus Faecalis V583  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Glucitol/sorbitol-specific phosphotransferase enzyme IIA component  
         PSI-BLAST Glucitol/sorbitol-specific phosphotransferase enzyme IIA component
    nr     (database updated : 2007.02.20 )
         BLAST glucitol/sorbitol-specific enzyme IIA component of PTS [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    EcoCyc srlB 

MMBR References
J Biol Chem. 1987;262:5455-5463
Glucitol-specific enzymes of the phosphotransferase system in Escherichia coli.
Yamada, M., and M. H. Saier.
J Bacteriol. 1987;169(7):2990-4.
Physical and genetic characterization of the glucitol operon in Escherichia coli.
Yamada M, Saier MH Jr. ( 3036766 )

Sequences
Amino acid
FASTA format
0001 MTVIYQTTIT RIGASAIDAL SDQMLITFRE GAPADLEEYC FIHCHGELKG ALHPGLQFSL GQHRYPVTAV 
0071 GSVAEDNLRE LGHVTLRFDG LNEAEFPGTV HVAGPVPDDI APGSVLKFES VKE

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             GGTGTCCCTT CTGTACTGGT GAGCCGCTTT 
-070 TTAACCGGCG CGCCGACTGT ACTGATCGCC TGGTTTGTCT CCGGTTTTAT CTATCAATAG AGGCTGAAAC 
0001 atgaccgtta tttatcagac caccatcacc cgtatcggcg cgagtgccat tgacgccctc agtgaccaga 
0071 tgctcatcac ctttcgtgaa ggcgcgcctg cggacctcga agagtattgc ttcattcatt gccacggcga 
0141 gttgaaaggt gcactccatc ccggtttgca attttcactc gggcagcatc gctatccggt gaccgctgtt 
0211 ggcagcgtgg cggaagacaa ccttcgcgaa ctgggtcatg tcaccctgcg cttcgatggt ttaaacgaag 
0281 cggaatttcc gggcactgtc catgtggcag gccctgtccc cgacgatatc gcgccgggat cggttttgaa 
0351 gtttgaatct gttaaggagt aaAAAATGAA TCAGGTTGCC GTTGTCATCG GTGGTGGGCA AACCTTAGGC 
0421 GCGTTCCTGT GCCACGGTCT GGCTGCCGAG GGGTATCGCG TCGCGGTTGT CG