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PEC Original Annotations
|
|
Essentiality
|
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Class
|
essential
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|
References (PMID)
|
J Bacteriol. 1984;159(2):783-6.
Deletion of an essential gene in Escherichia coli by site-specific recombination with linear DNA fragments.
Jasin M, Schimmel P.
(
6086588
)
|
|
|
Deletion
|
OCL48,49-2
(D)
,
OCL48,49-4 (BK6-8 / AK4-8)
(D)
|
|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
alaS
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Related strains
|
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Strains Search
|
Search strains by gene name:
alaS
Search strains by all related name:
alaS act ala-act b2697 ECK2692 JW2667 lovB
|
General information (Go to Linear View: )
|
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Gene Name
|
alaS
|
|
Alternative name
|
act,ala-act,b2697,ECK2692,JW2667,lovB
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|
Location, Length
|
2,817,403 - 2,820,033
(
-
)
;
60.72
min
;
2631
(bp) ,
876
(aa)
|
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Product
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alanyl-tRNA synthetase
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Operon Name
|
alaS
|
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation
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Function
|
enzyme; Aminoacyl tRNA synthetases, tRNA modification
|
|
Gene Ontology
|
GO:0000166
;
nucleotide binding ( alaS )
GO:0003676
;
nucleic acid binding ( alaS )
GO:0004812
;
aminoacyl-tRNA ligase activity ( alaS )
GO:0004813
;
alanine-tRNA ligase activity ( alaS )
GO:0005524
;
ATP binding ( alaS )
GO:0005624
;
membrane fraction ( alaS )
GO:0005737
;
cytoplasm ( alaS )
GO:0006412
;
translation ( alaS )
GO:0006419
;
alanyl-tRNA aminoacylation ( alaS )
GO:0008270
;
zinc ion binding ( alaS )
GO:0016874
;
ligase activity ( alaS )
GO:0016876
;
ligase activity, forming aminoacyl-tRNA and related compounds ( alaS )
GO:0043039
;
tRNA aminoacylation ( alaS )
GO:0046872
;
metal ion binding ( alaS )
|
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PID
|
1789048
|
EC number
(KEGG Pathway)
|
6.1.1.7
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
|
alaS
(
EG10034
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
SYA_ECOLI
(
P00957
;
P78279
)
|
|
- Protein name
|
Alanyl-tRNA synthetase
|
|
- Synonyms
|
EC 6.1.1.7, Alanine--tRNA ligase, AlaRS
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- Gene name
|
Name=alaS; Synonyms=lovB; OrderedLocusNames=b2697;
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Homology Analysis
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BLAST
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|
Bacteria
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GTOP
|
alaS
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
|
Chain A, Crystal Structure Of Alanyl-Trna Synthetase In Complex With Atp And Magnesium
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SWISS-PROT
(database updated :
2007.02.20
)
|
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BLAST
|
Alanyl-tRNA synthetase
|
|
PSI-BLAST
|
Alanyl-tRNA synthetase
|
|
nr
(database updated :
2007.02.20
)
|
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BLAST
|
alanyl-tRNA synthetase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
|
ASN_GLYCOSYLATION
GLYCOSAMINOGLYCAN
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
CYTOCHROME_C
AA_TRNA_LIGASE_II_2
|
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MMBR References
|
J Bacteriol. 1968;95(3):991-7.
Selection of temperature-sensitive activating enzyme mutants in Escherichia coli.
Kaplan S, Anderson D.
(
4868365
)
|
Biochem Biophys Res Commun. 1984;122(1):62-7.
The complete amino acid sequence of aspartate aminotransferase from Escherichia coli: sequence comparison with pig isoenzymes.
Kondo K, Wakabayashi S, Yagi T, Kagamiyama H.
(
6378205
)
|
J Biol Chem. 1981;256:205-211
Cloning, partial sequencing, and in vitro transcription of the gene for alanine tRNA synthetase.
Putney, S. D., D. L. Melendez, and P. Schimmel.
|
Science. 1981;213(4515):1497-501.
Primary structure of a large aminoacyl-tRNA synthetase.
Putney SD, Royal NJ, Neuman de Vegvar H, Herlihy WC, Biemann K, Schimmel P.
(
7025207
)
|
Nature. 1981;291(5817):632-5.
An aminoacyl tRNA synthetase binds to a specific DNA sequence and regulates its gene transcription.
Putney SD, Schimmel P.
(
6264314
)
|
EMBO J. 1989;8:317-323
The Escherichia coli lov gene product connects peptidoglycan synthesis, ribosomes and growth rate.
Bouloc, P., A. Jaffe, and R. D'Ari.
|
EMBO J. 1992;11:1493-1501
Penicillin binding protein 2 is dispensable in Escherichia coli when ppGpp synthesis is induced.
Vinella, D., R. D'Ari, and P. Bouloc.
|
| Sequences |
Amino acid
FASTA format
|
0001 MSKSTAEIRQ AFLDFFHSKG HQVVASSSLV PHNDPTLLFT NAGMNQFKDV FLGLDKRNYS RATTSQRCVR
0071 AGGKHNDLEN VGYTARHHTF FEMLGNFSFG DYFKHDAIQF AWELLTSEKW FALPKERLWV TVYESDDEAY
0141 EIWEKEVGIP RERIIRIGDN KGAPYASDNF WQMGDTGPCG PCTEIFYDHG DHIWGGPPGS PEEDGDRYIE
0211 IWNIVFMQFN RQADGTMEPL PKPSVDTGMG LERIAAVLQH VNSNYDIDLF RTLIQAVAKV TGATDLSNKS
0281 LRVIADHIRS CAFLIADGVM PSNENRGYVL RRIIRRAVRH GNMLGAKETF FYKLVGPLID VMGSAGEDLK
0351 RQQAQVEQVL KTEEEQFART LERGLALLDE ELAKLSGDTL DGETAFRLYD TYGFPVDLTA DVCRERNIKV
0421 DEAGFEAAME EQRRRAREAS GFGADYNAMI RVDSASEFKG YDHLELNGKV TALFVDGKAV DAINAGQEAV
0491 VVLDQTPFYA ESGGQVGDKG ELKGANFSFA VEDTQKYGQA IGHIGKLAAG SLKVGDAVQA DVDEARRARI
0561 RLNHSATHLM HAALRQVLGT HVSQKGSLVN DKVLRFDFSH NEAMKPEEIR AVEDLVNTQI RRNLPIETNI
0631 MDLEAAKAKG AMALFGEKYD ERVRVLSMGD FSTELCGGTH ASRTGDIGLF RIISESGTAA GVRRIEAVTG
0701 EGAIATVHAD SDRLSEVAHL LKGDSNNLAD KVRSVLERTR QLEKELQQLK EQAAAQESAN LSSKAIDVNG
0771 VKLLVSELSG VEPKMLRTMV DDLKNQLGST IIVLATVVEG KVSLIAGVSK DVTDRVKAGE LIGMVAQQVG
0841 GKGGGRPDMA QAGGTDAAAL PAALASVKGW VSAKLQ
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 aagaaaactt atcttattcc cacttttcag
-070 ttaccagccc ggcggttaag acacgctgga gctggtggcg atatttcgtt agcttgattt caggataatt
0001 atgagcaaga gcaccgctga gatccgtcag gcgtttctcg actttttcca tagtaaggga catcaggtag
0071 ttgccagcag ctccctggta ccccataacg acccaacttt gttgtttacc aacgccggga tgaaccagtt
0141 caaggatgtg ttccttgggc tcgacaagcg taattattcc cgcgctacca cttcccaacg ctgcgtgcgt
0211 gcgggtggta aacacaacga cctggaaaac gtcggttaca ccgcgcgtca ccataccttc ttcgaaatgc
0281 tgggcaactt cagcttcggc gactatttca aacacgatgc cattcagttt gcatgggaac tgctgaccag
0351 cgaaaaatgg tttgccctgc cgaaagagcg tctgtgggtt accgtctatg aaagcgacga cgaagcctac
0421 gaaatctggg aaaaagaagt agggatcccg cgcgaacgta ttattcgcat cggcgataac aaaggtgcgc
0491 catacgcatc tgacaacttc tggcagatgg gtgacactgg tccgtgcggc ccgtgcaccg aaatcttcta
0561 cgatcacggc gaccacattt gggggggccc tccgggaagc ccggaagaag acggcgaccg ctacattgag
0631 atctggaaca tcgtcttcat gcagttcaac cgccaggccg atggcacgat ggaaccgctg ccgaagccgt
0701 ctgtagatac cggtatgggt ctggagcgta ttgctgcggt gctgcaacac gttaactcta actatgacat
0771 cgacctgttc cgcacgctga tccaggcggt agcgaaagtc actggcgcaa ccgatctgag caataaatcg
0841 ctgcgcgtaa tcgctgacca cattcgttct tgtgcgttcc tgatcgcgga tggcgtaatg ccgtccaatg
0911 aaaaccgtgg ttatgtactg cgtcgtatca ttcgtcgcgc agtgcgtcac ggtaatatgc tcggcgcgaa
0981 agaaaccttc ttctacaaac tggttggtcc gctgatcgac gttatgggct ctgcgggtga agacctgaaa
1051 cgccagcagg cgcaggttga gcaggtgctg aagactgaag aagagcagtt tgctcgtact ctggagcgcg
1121 gtctggcgtt gctggatgaa gagctggcaa aactttctgg tgatacgctg gatggtgaaa ctgctttccg
1191 tctgtacgac acctatggct tcccggttga cctgacggct gatgtttgtc gtgagcgcaa catcaaagtt
1261 gacgaagctg gttttgaagc tgcaatggaa gagcagcgtc gtcgcgcgcg cgaagccagc ggctttggtg
1331 ccgattacaa cgcaatgatc cgtgttgaca gtgcatctga atttaaaggc tatgaccatc tggaactgaa
1401 cggcaaagtg actgcgctgt ttgttgatgg taaagcggtt gatgccatca atgcaggcca ggaagctgtg
1471 gtcgtgctgg atcaaacgcc attctatgcg gaatccggcg gtcaggttgg cgataaaggc gaactgaaag
1541 gcgctaactt ctcctttgcg gtggaagata cgcagaaata cggccaggcg attggtcaca tcggtaaact
1611 tgctgcgggt tctctgaaag tgggcgacgc ggtgcaggct gatgttgatg aggctcgtcg cgcccgtatt
1681 cgtctgaatc actccgcaac gcacctgatg cacgctgcgc tgcgccaggt tctgggtact catgtatcgc
1751 agaaaggttc actggttaac gacaaggtgc tgcgcttcga cttctcacac aacgaagcga tgaaaccaga
1821 agagattcgt gcggtcgaag acctggtgaa cacacagatt cgtcgcaatt tgccgatcga aaccaacatc
1891 atggatctcg aagcggcgaa agcgaaaggt gcgatggcgc tgttcggcga gaagtatgat gagcgcgtac
1961 gcgtgctgag catgggcgat ttctctaccg agttgtgtgg cggtactcac gccagccgca ctggtgatat
2031 tggtctgttc cgcatcatct ctgaatcggg tactgctgca ggcgttcgtc gtatcgaagc ggtaaccgga
2101 gaaggtgcta tcgccaccgt tcatgcagac agcgatcgct taagcgaagt cgcgcatctg ctgaaaggcg
2171 atagcaataa tctggctgat aaagtgcgct cagtactgga acgtacgcgt cagctggaaa aagagttaca
2241 acagcttaaa gaacaagctg ccgcacagga gagcgcaaat ctttccagta aggcaattga tgttaatggt
2311 gttaagctgt tggttagcga gcttagcggt gttgagccga aaatgttgcg taccatggtt gacgatttaa
2381 aaaatcagct ggggtcgaca attatcgtgc tggcaacggt agtcgaaggt aaggtttctc tgattgcagg
2451 cgtatctaag gacgtcacag atcgtgtgaa agcaggggaa ctgattggta tggtcgctca gcaggtgggc
2521 ggcaagggtg gtggacgtcc tgacatggcg caagccggtg gtacggatgc tgcggcctta cctgcagcgt
2591 tagccagtgt gaaaggctgg gtcagcgcga aattgcaata atataagcgt caggcaatgc cgtggactcg
2661 cttcacggca ttcgcattaa cgctatcgac aacgataaag tcaggttgaa gttgtgtata tcggctaaac
2731 t
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