MG1655
W3110
Search for
Japanese | English
Gene Report : proX
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion 22 (LD)  ,  OCL49,51-3 (LD 22B_CRS) (D)  ,  OCL49,51-4 (LD 22B_P/O) (D)  ,  OCL49-1 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  proX
Related strains
     Strains Search Search strains by gene name:  proX   Search strains by all related name:  proX b2679 ECK2673 JW2654 osrA proU

General information  (Go to Linear View:)
 Gene Name proX  
 Alternative name b2679,ECK2673,JW2654,osrA,proU  
 Location, Length 2,805,154 - 2,806,146 (  +  ) ;   60.46 min ; 993 (bp) ,   330 (aa) Go to Linear View
 Product glycine betaine transporter subunit  
 Operon Name proVWX  
 Note high-affinity transport system for glycine betaine and proline; periplasmic-binding component of ABC superfamily; GO_component: GO:0042597 - periplasmic space;GO_function: GO:0015596 - glycine betaine/proline porter activity; GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0006561 - proline biosynthetic process; GO_process: GO:0006545 - glycine biosynthetic process; GO_process: GO:0006578 - betaine biosynthetic process; GO_process: GO:0006970 - response to osmotic stress  
 Function transport; Osmotic adaptation  
 Gene Ontology GO:0005215; transporter activity ( proX )
GO:0005488; binding ( proX )
GO:0005515; protein binding ( proX )
GO:0006810; transport ( proX )
GO:0006865; amino acid transport ( proX )
GO:0042597; periplasmic space ( proX )
 PID 1789034  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene proX ( EG10773 )  
    Number of removed 21 aa cleaved  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) PROX_ECOLI ( P14177 )
    -  Protein name Glycine betaine-binding periplasmic protein precursor.  
    -  Synonyms  
    -  Gene name Name=proX; Synonyms=proU; OrderedLocusNames=b2679, z3981, ECs3542;  

 Linear View (Whole Mode)
View Location
   2795.0  –  2820.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP proX (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Structure Analysis Of Prox In Complex With Glycine Betaine  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Glycine betaine-binding periplasmic protein precursor  
         PSI-BLAST Glycine betaine-binding periplasmic protein precursor
    nr     (database updated : 2007.02.20 )
         BLAST high-affinity glycine betaine/proline transport system protein [Escherichia coli O157:H7 str. Sakai]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    PROKAR_LIPOPROTEIN     

Other Cross-Reference
    COG COG2113E 
    EcoCyc proX 

MMBR References
J Bacteriol. 1986;166(1):253-9.
Proline transport and osmotic stress response in Escherichia coli K-12.
Grothe S, Krogsrud RL, McClellan DJ, Milner JL, Wood JM. ( 3514577 )
Mol Microbiol. 1989;3(8):1025-38.
Molecular characterization of the proU loci of Salmonella typhimurium and Escherichia coli encoding osmoregulated glycine betaine transport systems.
Stirling DA, Hulton CS, Waddell L, Park SF, Stewart GS, Booth IR, Higgins CF. ( 2691838 )

Sequences
Amino acid
FASTA format
0001 MRHSVLFATA FATLISTQTF AADLPGKGIT VNPVQSTITE ETFQTLLVSR ALEKLGYTVN KPSEVDYNVG 
0071 YTSLASGDAT FTAVNWTPLH DNMYEAAGGD KKFYREGVFV NGAAQGYLID KKTADQYKIT NIAQLKDPKI 
0141 AKLFDTNGDG KADLTGCNPG WGCEGAINHQ LAAYELTNTV THNQGNYAAM MADTISRYKE GKPVFYYTWT 
0211 PYWVSNELKP GKDVVWLQVP FSALPGDKNA DTKLPNGANY GFPVSTMHIV ANKAWAEKNP AAAKLFAIMQ 
0281 LPVADINAQN AIMHDGKASE GDIQGHVDGW IKAHQQQFDG WVNEALAAQK 

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             CACTGGCCCT GTTGGTCTGC TGACCCGCCC 
-070 ATTCATTAAG TAACTCTGCA CTTGCCCGGT GACGCCGGGC ATTATCACCC TGCCAAAAAA AGGAATAACA 
0001 atgcgacata gcgtactttt tgcgacagcg tttgccacgc ttatctctac acaaactttt gctgccgatc 
0071 tgccgggcaa aggcattact gttaatccag ttcagagcac catcactgaa gaaaccttcc agacgctgct 
0141 ggtcagtcgt gcgctggaga aattaggtta taccgtcaac aaacccagcg aagtagatta caacgttggc 
0211 tacacctcgc ttgcttccgg cgatgcaacc ttcaccgccg tgaactggac gccactgcat gacaacatgt 
0281 acgaagctgc cggtggcgat aagaaatttt atcgtgaagg ggtatttgtt aacggcgcgg cacagggtta 
0351 cctgatcgat aagaaaaccg ccgaccagta caaaatcacc aacatcgcac aactgaaaga tccgaagatc 
0421 gccaaactgt tcgataccaa cggcgacgga aaagcggatt taaccggttg taaccctggc tggggctgcg 
0491 aaggtgcgat caaccaccag cttgccgcgt atgaactgac caacaccgtg acgcataatc aggggaacta 
0561 cgcagcgatg atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg tgttttatta cacctggacg 
0631 ccgtactggg tgagtaacga actgaagccg ggcaaagatg tcgtctggtt gcaggtgccg ttctccgcac 
0701 tgccgggcga taaaaacgcc gataccaaac tgccgaatgg tgcgaattat ggcttcccgg tcagcaccat 
0771 gcatatcgtt gccaacaaag cctgggccga gaaaaacccg gcagcagcga aactgtttgc cattatgcag 
0841 ttgccagtgg cagatattaa cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc 
0911 agggacacgt tgatggttgg atcaaagccc accagcagca gttcgatggc tgggtgaatg aggcgctggc 
0981 agcgcagaag taaTTTTTAT TCGGGCGGAT AAGGCGTTTT TGCCGCATCC GCCGTTCTGT GCACAATGCC 
1051 TGATGCGACG CTGGCGCGTC TTATCATGCC TACAAACCAC ATC