MG1655
W3110
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Gene Report : proW
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion 22 (LD)  ,  OCL49,51-3 (LD 22B_CRS) (D)  ,  OCL49,51-4 (LD 22B_P/O) (D)  ,  OCL49-1 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  proW
Related strains
     Strains Search Search strains by gene name:  proW   Search strains by all related name:  proW b2678 ECK2672 JW2653 o354 osrA proU

General information  (Go to Linear View:)
 Gene Name proW  
 Alternative name b2678,ECK2672,JW2653,o354,osrA,proU  
 Location, Length 2,804,032 - 2,805,096 (  +  ) ;   60.44 min ; 1065 (bp) ,   354 (aa) Go to Linear View
 Product glycine betaine transporter subunit  
 Operon Name proVWX  
 Note high-affinity transport system for glycine betaine and proline; membrane component of ABC superfamily; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane;GO_function: GO:0015596 - glycine betaine/proline porter activity; GO_process: GO:0006561 - proline biosynthetic process  
 Function transport; Transport of small molecules: Amino acids, amines  
 Gene Ontology GO:0005215; transporter activity ( proW )
GO:0005886; plasma membrane ( proW )
GO:0006810; transport ( proW )
GO:0006865; amino acid transport ( proW )
GO:0016020; membrane ( proW )
GO:0016021; integral to membrane ( proW )
 PID 1789033  
 EC number
  (KEGG Pathway)
 
 
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) PROW_ECOLI ( P14176 )
    -  Protein name Glycine betaine/L-proline transport system permease protein proW.  
    -  Synonyms  
    -  Gene name Name=proW; OrderedLocusNames=b2678;  

 Linear View (Whole Mode)
View Location
   2795.0  –  2820.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP proW (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Analysis Of Sup35  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Glycine betaine/L-proline transport system permease protein proW  
         PSI-BLAST Glycine betaine/L-proline transport system permease protein proW
    nr     (database updated : 2007.02.20 )
         BLAST Glycine betaine/L-proline transport system permease protein proW [Escherichia coli CFT073]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    PKC_PHOSPHO_SITE    MYRISTYL    BPD_TRANSP_INN_MEMBR     

Other Cross-Reference
    COG COG4176E 
    EcoCyc proW 

MMBR References
J Bacteriol. 1986;166(1):253-9.
Proline transport and osmotic stress response in Escherichia coli K-12.
Grothe S, Krogsrud RL, McClellan DJ, Milner JL, Wood JM. ( 3514577 )
J Bacteriol. 1996;178:5370-5381
Use of phoA and lacZ fusions to study the membrane topology of ProW, a component of the osmoregulated ProU transport system of Escherichia coli.
Haardt, M., and E. Bremer.
Mol Microbiol. 1989;3(8):1025-38.
Molecular characterization of the proU loci of Salmonella typhimurium and Escherichia coli encoding osmoregulated glycine betaine transport systems.
Stirling DA, Hulton CS, Waddell L, Park SF, Stewart GS, Booth IR, Higgins CF. ( 2691838 )

Sequences
Amino acid
FASTA format
0001 MADQNNPWDT TPAADSAAQS ADAWGTPTTA PTDGGGADWL TSTPAPNVEH FNILDPFHKT LIPLDSWVTE 
0071 GIDWVVTHFR PVFQGVRVPV DYILNGFQQL LLGMPAPVAI IVFALIAWQI SGVGMGVATL VSLIAIGAIG 
0141 AWSQAMVTLA LVLTALLFCI VIGLPLGIWL ARSPRAAKII RPLLDAMQTT PAFVYLVPIV MLFGIGNVPG 
0211 VVVTIIFALP PIIRLTILGI NQVPADLIEA SRSFGASPRQ MLFKVQLPLA MPTIMAGVNQ TLMLALSMVV 
0281 IASMIAVGGL GQMVLRGIGR LDMGLATVGG VGIVILAIIL DRLTQAVGRD SRSRGNRRWY TTGPVGLLTR 
0351 PFIK

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             GCACCCTGTG CGGTGCCCGT GGTCGACGAG 
-070 GACCAACAGT ATGTCGGCAT CATTTCGAAA GGAATGCTGC TGCGCGCTTT AGATCGTGAG GGGGTAAATA 
0001 atggctgatc aaaataatcc gtgggatacc acgccagcgg cggacagtgc cgcgcaatcc gcagacgcct 
0071 ggggtacacc gacgactgca ccgactgacg gcggtggtgc tgactggctg accagtacgc ctgcgccaaa 
0141 cgtcgagcat tttaatattc tcgatccgtt ccataaaacg ctgatcccgc tcgacagttg ggtcactgaa 
0211 gggatcgact gggtcgttac ccatttccgt cccgtcttcc agggcgtgcg cgttccggtt gattatatcc 
0281 tcaacggttt ccagcaattg ctgctgggta tgcccgcacc ggtggcgatt atcgttttcg ctctcatcgc 
0351 ctggcagatt tccggggtcg gaatgggtgt ggcgacgctg gtttcgctga ttgccatcgg cgcaatcggt 
0421 gcctggtcgc aggcaatggt gactctggcg ctggtgttaa ccgccctgct gttctgtatc gtcatcggtt 
0491 tgccgttggg gatatggctg gcgagaagtc cgcgagcggc gaaaattatt cgtccactgc ttgatgccat 
0561 gcagaccacg ccagcgtttg tttatctggt gccaatcgtc atgctatttg gtatcggtaa cgtgccgggc 
0631 gtggtggtga cgatcatctt tgctctgccg ccgattatcc gtctgaccat tctggggatt aaccaggttc 
0701 cggcggatct gattgaagcc tcgcgctcat tcggtgccag cccgcgccag atgctgttca aagttcagtt 
0771 accgctggcg atgccgacca ttatggcggg cgttaaccag acgctgatgc tggccctttc tatggtggtc 
0841 atcgcctcga tgattgccgt cggcgggttg ggtcagatgg tacttcgcgg tatcggtcgt ctggatatgg 
0911 ggcttgccac cgttggcggc gtcgggattg tgatcctcgc cattatcctc gatcgtctga cgcaggccgt 
0981 tgggcgcgac tcacgcagtc gcggcaaccg tcgctggtac accactggcc ctgttggtct gctgacccgc 
1051 ccattcatta agtaaCTCTG CACTTGCCCG GTGACGCCGG GCATTATCAC CCTGCCAAAA AAAGGAATAA 
1121 CAATGCGACA TAGCGTACTT TTTGCGACAG CGTTTGCCAC GCTTA