MG1655
W3110
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Gene Report : proV
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion 22 (LD)  ,  OCL49,51-3 (LD 22B_CRS) (D)  ,  OCL49,51-4 (LD 22B_P/O) (D)  ,  OCL49-1 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  proV
Related strains
     Strains Search Search strains by gene name:  proV   Search strains by all related name:  proV b2677 ECK2671 JW2652 o400 osrA proU

General information  (Go to Linear View:)
 Gene Name proV  
 Alternative name b2677,ECK2671,JW2652,o400,osrA,proU  
 Location, Length 2,802,837 - 2,804,039 (  +  ) ;   60.41 min ; 1203 (bp) ,   400 (aa) Go to Linear View
 Product glycine betaine transporter subunit  
 Operon Name proVWX  
 Note ATP-binding component of transport system for glycine, betaine and proline; GO_component: GO:0005737 - cytoplasm;GO_function: GO:0015596 - glycine betaine/proline porter activity; GO_process: GO:0006561 - proline biosynthetic process  
 Function transport; Transport of small molecules: Amino acids, amines  
 Gene Ontology GO:0000166; nucleotide binding ( proV )
GO:0005515; protein binding ( proV )
GO:0005524; ATP binding ( proV )
GO:0005886; plasma membrane ( proV )
GO:0006810; transport ( proV )
GO:0006865; amino acid transport ( proV )
GO:0015171; amino acid transmembrane transporter activity ( proV )
GO:0016020; membrane ( proV )
GO:0016887; ATPase activity ( proV )
GO:0017111; nucleoside-triphosphatase activity ( proV )
 PID 1789032  
 EC number
  (KEGG Pathway)
 
 
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) PROV_ECOLI ( P14175 )
    -  Protein name Glycine betaine/L-proline transport ATP-binding protein proV.  
    -  Synonyms  
    -  Gene name Name=proV; OrderedLocusNames=b2677;  

 Linear View (Whole Mode)
View Location
   2790.0  –  2815.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP proV (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain 1, Malk  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Glycine betaine/L-proline transport ATP-binding protein proV  
         PSI-BLAST Methionine import ATP-binding protein metN
    nr     (database updated : 2007.02.20 )
         BLAST glycine betaine transporter subunit [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE GLYCOSAMINOGLYCAN    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    ATP_GTP_A    ABC_TRANSPORTER     

Other Cross-Reference
    COG COG4175E 
    EcoCyc proV 

MMBR References
Mol Microbiol. 1988;2(2):265-79.
Cloned structural genes for the osmotically regulated binding-protein-dependent glycine betaine transport system (ProU) of Escherichia coli K-12.
Faatz E, Middendorf A, Bremer E. ( 2837616 )
Microbiol. Rev. 1985;49:379-397.
Structure and organization of the transfer ribonucleic acid genes of Escherichia coli K-12.
Fournier, M. J., and H. Ozeki.
J Bacteriol. 1989;171(4):1923-31.
Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli.
Gowrishankar J. ( 2649479 )
J Bacteriol. 1986;168(3):1197-204.
Molecular cloning of an osmoregulatory locus in Escherichia coli: increased proU gene dosage results in enhanced osmotolerance.
Gowrishankar J, Jayashree P, Rajkumari K. ( 3536868 )
J Bacteriol. 1986;166(1):253-9.
Proline transport and osmotic stress response in Escherichia coli K-12.
Grothe S, Krogsrud RL, McClellan DJ, Milner JL, Wood JM. ( 3514577 )
Mol Microbiol. 1989;3(11):1521-31.
Characterization of the osmoregulated Escherichia coli proU promoter and identification of ProV as a membrane-associated protein.
May G, Faatz E, Lucht JM, Haardt M, Bolliger M, Bremer E. ( 2515417 )
Mol Microbiol. 1989;3(8):1025-38.
Molecular characterization of the proU loci of Salmonella typhimurium and Escherichia coli encoding osmoregulated glycine betaine transport systems.
Stirling DA, Hulton CS, Waddell L, Park SF, Stewart GS, Booth IR, Higgins CF. ( 2691838 )
J Bacteriol. 1986;168(2):805-14.
Osmotic regulation of transcription: induction of the proU betaine transport gene is dependent on accumulation of intracellular potassium.
Sutherland L, Cairney J, Elmore MJ, Booth IR, Higgins CF. ( 3536861 )
J Bacteriol. 1989;171(4):1915-22.
Osmoregulation in Escherichia coli: complementation analysis and gene-protein relationships in the proU locus.
Dattananda CS, Gowrishankar J. ( 2649478 )

Sequences
Amino acid
FASTA format
0001 MAIKLEIKNL YKIFGEHPQR AFKYIEQGLS KEQILEKTGL SLGVKDASLA IEEGEIFVIM GLSGSGKSTM 
0071 VRLLNRLIEP TRGQVLIDGV DIAKISDAEL REVRRKKIAM VFQSFALMPH MTVLDNTAFG MELAGINAEE 
0141 RREKALDALR QVGLENYAHS YPDELSGGMR QRVGLARALA INPDILLMDE AFSALDPLIR TEMQDELVKL 
0211 QAKHQRTIVF ISHDLDEAMR IGDRIAIMQN GEVVQVGTPD EILNNPANDY VRTFFRGVDI SQVFSAKDIA 
0281 RRTPNGLIRK TPGFGPRSAL KLLQDEDREY GYVIERGNKF VGAVSIDSLK TALTQQQGLD AALIDAPLAV 
0351 DAQTPLSELL SHVGQAPCAV PVVDEDQQYV GIISKGMLLR ALDREGVNNG 

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             AGGGGTTGCC TCAGATTCTC AGTATGTTAG 
-070 GGTAGAAAAA AGTGACTATT TCCATTGGGT AATATATCGA CATAGACAAA TAAAGGAATC TTTCTATTGC 
0001 atggcaatta aattagaaat taaaaatctt tataaaatat ttggcgagca tccacagcga gcgttcaaat 
0071 atatcgaaca aggactttca aaagaacaaa ttctggaaaa aactgggcta tcgcttggcg taaaagacgc 
0141 cagtctggcc attgaagaag gcgagatatt tgtcatcatg ggattatccg gctcgggtaa atccacaatg 
0211 gtacgccttc tcaatcgcct gattgaaccc acccgcgggc aagtgctgat tgatggtgtg gatattgcca 
0281 aaatatccga cgccgaactc cgtgaggtgc gcagaaaaaa gattgcgatg gtcttccagt cctttgcctt 
0351 aatgccgcat atgaccgtgc tggacaatac tgcgttcggt atggaattgg ccggaattaa tgccgaagaa 
0421 cgccgggaaa aagcccttga tgcactgcgt caggtcgggc tggaaaatta tgcccacagc tacccggatg 
0491 aactctctgg cgggatgcgt caacgtgtgg gattagcccg cgcgttagcg attaatccgg atatattatt 
0561 aatggacgaa gccttctcgg cgctcgatcc attaattcgc accgagatgc aggatgagct ggtaaaatta 
0631 caggcgaaac atcagcgcac cattgtcttt atttcccacg atcttgatga agccatgcgt attggcgacc 
0701 gaattgccat tatgcaaaat ggtgaagtgg tacaggtcgg cacaccggat gaaattctca ataatccggc 
0771 gaatgattat gtccgtacct tcttccgtgg cgttgatatt agtcaggtat tcagtgcgaa agatattgcc 
0841 cgccggacac cgaatggctt aattcgtaaa acccctggct tcggcccacg ttcggcactg aaattattgc 
0911 aggatgaaga tcgcgaatat ggctacgtta tcgaacgcgg taataagttt gtcggcgcag tctccatcga 
0981 ttcgcttaaa accgcgttaa cgcagcagca aggtcttgat gcggcgctga ttgatgcgcc gttagcagtc 
1051 gatgcacaaa cgcctcttag cgagttgctc tctcatgtcg gacaggcacc ctgtgcggtg cccgtggtcg 
1121 acgaggacca acagtatgtc ggcatcattt cgaaaggaat gctgctgcgc gctttagatc gtgagggggt 
1191 aaataatggc tgaTCAAAAT AATCCGTGGG ATACCACGCC AGCGGCGGAC AGTGCCGCGC AATCCGCAGA 
1261 CGCCTGGGGT ACACCGACGA CTGCACCGAC TGACGGCGGT GGT