MG1655
W3110
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Gene Report : recN
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCL51 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  recN
Related strains
     Strains Search Search strains by gene name:  recN   Search strains by all related name:  recN b2616 ECK2612 JW5416 o553 radB

General information  (Go to Linear View:)
 Gene Name recN  
 Alternative name b2616,ECK2612,JW5416,o553,radB  
 Location, Length 2,749,817 - 2,751,478 (  +  ) ;   59.27 min ; 1662 (bp) ,   553 (aa) Go to Linear View
 Product recombination and repair protein  
 Operon Name recN  
 Note protein used in recombination and DNA repair; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006310 - DNA recombination; GO_process: GO:0006281 - DNA repair; GO_process: GO:0009432 - SOS response  
 Function putative enzyme; DNA - replication, repair,restriction/modification  
 Gene Ontology GO:0000166; nucleotide binding ( recN )
GO:0005515; protein binding ( recN )
GO:0005524; ATP binding ( recN )
GO:0005694; chromosome ( recN )
GO:0006259; DNA metabolic process ( recN )
GO:0006281; DNA repair ( recN )
GO:0006310; DNA recombination ( recN )
GO:0006974; response to DNA damage stimulus ( recN )
 PID 48994901  
 EC number
  (KEGG Pathway)
 
 
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) RECN_ECOLI ( P05824 ; P76602 )
    -  Protein name DNA repair protein recN  
    -  Synonyms Recombination protein N  
    -  Gene name Name=recN; Synonyms=radB; OrderedLocusNames=b2616;  

 Linear View (Whole Mode)
View Location
   2740.0  –  2765.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP recN (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Smc Head Domain From Thermotoga Maritima  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST DNA repair protein recN  
         PSI-BLAST Keratin type II cuticular Hb4
    nr     (database updated : 2007.02.20 )
         BLAST recombination and repair protein [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    ATP_GTP_A     

Other Cross-Reference
    COG COG0497L 
    EcoCyc recN 

MMBR References
Mol Gen Genet. 1983;190(1):162-7.
Inducible expression of a gene specific to the RecF pathway for recombination in Escherichia coli K12.
Lloyd RG, Picksley SM, Prescott C. ( 6343801 )
J Bacteriol. 1991;173(11):3271-2.
Two new genes located between 2758 and 2761 kilobase pairs on the Escherichia coli genome.
Miczak A, Chauhan AK, Apirion D. ( 2045357 )
Mol Gen Genet. 1985;201(2):301-7.
The recN locus of Escherichia coli K12: molecular analysis and identification of the gene product.
Picksley SM, Morton SJ, Lloyd RG. ( 3003532 )
Nucleic Acids Res. 1987;15:5041-5049
Nucleotide sequence and LexA regulation of the Escherichia coli recN gene.
Rostas, K., S. J. Morton, S. M. Picksley, and R. G. Lloyd.
Radiat Res. 1983;93(3):461-78.
Characterization of an Escherichia coli mutant (radB101) sensitive to gamma and uv radiation, and methyl methanesulfonate.
Sargentini NJ, Smith KC. ( 6344125 )
J Bacteriol. 1988;170(5):2392-4.
Genetic and phenotypic analyses indicating occurrence of the recN262 and radB101 mutations at the same locus in Escherichia coli.
Sargentini NJ, Smith KC. ( 3283110 )

Sequences
Amino acid
FASTA format
0001 MLAQLTISNF AIVRELEIDF HSGMTVITGE TGAGKSIAID ALGLCLGGRA EADMVRTGAA RADLCARFSL 
0071 KDTPAALRWL EENQLEDGHE CLLRRVISSD GRSRGFINGT AVPLSQLREL GQLLIQIHGQ HAHQLLTKPE 
0141 HQKFLLDGYA NETSLLQEMT ARYQLWHQSC RDLAHHQQLS QERAARAELL QYQLKELNEF NPQPGEFEQI 
0211 DEEYKRLANS GQLLTTSQNA LALMADGEDA NLQSQLYTAK QLVSELIGMD SKLSGVLDML EEATIQIAEA 
0281 SDELRHYCDR LDLDPNRLFE LEQRISKQIS LARKHHVSPE ALPQYYQSLL EEQQQLDDQA DSQETLALAV 
0351 TKHHQQALEI ARALHQQRQQ YAEELAQLIT DSMHALSMPH GQFTIDVKFD EHHLGADGAD RIEFRVTTNP 
0421 GQPMQPIAKV ASGGELSRIA LAIQVITARK METPALIFDE VDVGISGPTA AVVGKLLRQL GESTQVMCVT 
0491 HLPQVAGCGH QHYFVSKETD GAMTETHMQS LNKKARLQEL ARLLGGSEVT RNTLANAKEL LAA

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             AAAAAATTAT TCTAATTTTA CGCCAGCCTC 
-070 TTTACTGTAT ATAAAACCAG TTTATACTGT ACACAATAAC AGTAATGGTT TTTCATACAG GAAAACGACT 
0001 atgttggcac aactgaccat cagcaacttt gctatcgttc gtgagcttga gattgatttt catagcggca 
0071 tgaccgtaat aactggcgag accggcgcgg gtaaatctat tgcaatagat gccctcggtc tttgtctcgg 
0141 tggtcgcgct gaagccgaca tggtgcgtac cggcgctgct cgcgctgacc tgtgcgcccg tttttctctg 
0211 aaagatacgc cagcggctct gcgctggctg gaagaaaacc agcttgaaga cgggcatgaa tgtttgcttc 
0281 gtcgcgtgat cagcagcgat ggtcgctccc gtggtttcat caacggtaca gctgttcctc tgtcacaact 
0351 gcgcgaactg ggtcagttgc tgattcagat ccatggtcag cacgctcatc aattactcac caaacctgag 
0421 caccaaaaat tcctgcttga tggctatgcc aatgaaacct ctctactgca ggaaatgacc gcacgttatc 
0491 agttgtggca tcaaagctgc cgtgacctcg cgcatcatca acagttaagt caggaacgcg ccgcccgtgc 
0561 ggaactgctg caataccaat taaaagaact taacgaattt aatccgcagc ccggagagtt tgaacaaatc 
0631 gacgaagagt acaaacgtct ggcgaacagc ggtcaattgc tgaccaccag ccagaatgca ttggcattaa 
0701 tggccgacgg tgaagacgca aacctgcaaa gtcagcttta cacggctaaa caactggtga gcgaattgat 
0771 tggcatggac agcaaactgt ccggcgtact tgatatgctg gaagaagcta ccatccagat tgctgaagcc 
0841 agcgatgaac tgcgccacta ctgcgatcgt ctggatctcg atcccaaccg actatttgaa cttgaacagc 
0911 gcatctcaaa acagatttcg ctggcacgta aacatcacgt cagccctgag gcattgccac agtattacca 
0981 gtcgctactg gaagaacagc agcaactgga cgatcaggcc gactcacaag aaacgcttgc gctggcggta 
1051 acgaaacatc atcagcaggc actggaaatc gcgcgcgcat tacaccaaca acgccagcaa tatgcagaag 
1121 aacttgcaca gctgatcacc gacagtatgc atgcgctctc aatgccgcat gggcagttta cgatcgatgt 
1191 taaatttgac gagcatcacc tgggcgctga cggtgccgat cgtattgagt ttcgggtaac caccaaccca 
1261 ggtcagccaa tgcagcctat tgccaaagtc gcatccggtg gtgaattgtc ccgcatcgca ctggcaatcc 
1331 aggtcatcac ggcgcgtaaa atggaaaccc cggcactgat ttttgatgaa gtggatgtag ggattagcgg 
1401 tccaacagcg gcagttgtcg gcaaactgct gcgtcaactc ggcgaatcaa ctcaggtgat gtgtgttacc 
1471 cacctgccac aagtcgcggg atgtggtcat caacactatt ttgtcagcaa agaaaccgat ggtgcgatga 
1541 cagaaacgca tatgcaatcc ctgaataaaa aagcgcggtt acaagagctg gcgcgcctgc ttggtggcag 
1611 tgaagtcaca cgtaatacac tggcgaatgc gaaagaactg cttgcagcgt aaACTTTTTT CCTGCTTCAC 
1681 GGTCAGAGTA AACAGCAAAA CGCCGTAAGA CCGGAAAGCA AAAGGTTTTA AAGTGATGAA AGGTCTATTA 
1751 TCATCGGCAT AT