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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
|
J Bacteriol. 1986;166(1):83-7.
lep operon proximal gene is not required for growth or secretion by Escherichia coli.
Dibb NJ, Wolfe PB.
(
3514582
)
|
|
|
Deletion
|
OCL53,54-1
(D)
|
|
Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
lepA
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Related strains
|
|
Strains Search
|
Search strains by gene name:
lepA
Search strains by all related name:
lepA b2569 ECK2567 JW2553
|
General information (Go to Linear View: )
|
|
Gene Name
|
lepA
|
|
Alternative name
|
b2569,ECK2567,JW2553
|
|
Location, Length
|
2,703,347 - 2,705,146
(
-
)
;
58.27
min
;
1800
(bp) ,
599
(aa)
|
|
Product
|
back-translocating elongation factor EF4, GTPase
|
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Operon Name
|
lepAB
|
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Note
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GTP-binding elongation factor, may be inner membrane protein; GO_process: GO:0006412 - translation
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Function
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factor; Proteins - translation and modification
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Gene Ontology
|
GO:0000166
;
nucleotide binding ( lepA )
GO:0003924
;
GTPase activity ( lepA )
GO:0005525
;
GTP binding ( lepA )
GO:0005886
;
plasma membrane ( lepA )
GO:0007264
;
small GTPase mediated signal transduction ( lepA )
GO:0016020
;
membrane ( lepA )
GO:0042597
;
periplasmic space ( lepA )
|
|
PID
|
1788922
|
EC number
(KEGG Pathway)
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|
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
LEPA_ECOLI
(
P60785
;
P07682; P76590
)
|
|
- Protein name
|
GTP-binding protein lepA.
|
|
- Synonyms
|
|
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- Gene name
|
Name=lepA; OrderedLocusNames=b2569;
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Homology Analysis
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BLAST
|
|
Bacteria
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GTOP
|
lepA
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
|
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PSI-BLAST
|
Chain B, Coordinates Of Rrf And Ef-G Fitted Into Cryo-Em Map Of The 50s Subunit Bound With Both Ef-G
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
GTP-binding protein lepA
|
|
PSI-BLAST
|
GTP-binding protein lepA
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
GTP-binding elongation factor [Shigella sonnei Ss046]
|
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Pfam 28.0
(database updated :
2015-05
)
|
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Pfam
|
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PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AMIDATION
ATP_GTP_A
EFACTOR_GTP
|
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MMBR References
|
J Bacteriol. 1986;166(1):83-7.
lep operon proximal gene is not required for growth or secretion by Escherichia coli.
Dibb NJ, Wolfe PB.
(
3514582
)
|
J Biol Chem. 1985;260:7206-7213
Characterization of the lep operon of Escherichia coli. Identification of the promoter and the gene upstream of the signal peptidase I gene.
March, P. E., and M. Inoue.
|
Proc Natl Acad Sci U S A. 1985;82:7500-7504
GTP-binding membrane protein of Escherichia coli with sequence homology to initiation factor 2 and elongation factors Tu and G.
March, P. E., and M. Inouye.
|
J Bacteriol. 1983;154(2):569-72.
Genetic mapping of the Escherichia coli leader (signal) peptidase gene (lep): a new approach for determining the map position of a cloned gene.
Silver P, Wickner W.
(
6341355
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MKNIRNFSII AHIDHGKSTL SDRIIQICGG LSDREMEAQV LDSMDLERER GITIKAQSVT LDYKASDGET
0071 YQLNFIDTPG HVDFSYEVSR SLAACEGALL VVDAGQGVEA QTLANCYTAM EMDLEVVPVL NKIDLPAADP
0141 ERVAEEIEDI VGIDATDAVR CSAKTGVGVQ DVLERLVRDI PPPEGDPEGP LQALIIDSWF DNYLGVVSLI
0211 RIKNGTLRKG DKVKVMSTGQ TYNADRLGIF TPKQVDRTEL KCGEVGWLVC AIKDIHGAPV GDTLTLARNP
0281 AEKALPGFKK VKPQVYAGLF PVSSDDYEAF RDALGKLSLN DASLFYEPES SSALGFGFRC GFLGLLHMEI
0351 IQERLEREYD LDLITTAPTV VYEVETTSRE VIYVDSPSKL PAVNNIYELR EPIAECHMLL PQAYLGNVIT
0421 LCVEKRGVQT NMVYHGNQVA LTYEIPMAEV VLDFFDRLKS TSRGYASLDY NFKRFQASDM VRVDVLINGE
0491 RVDALALITH RDNSQNRGRE LVEKMKDLIP RQQFDIAIQA AIGTHIIARS TVKQLRKNVL AKCYGGDISR
0561 KKKLLQKQKE GKKRMKQIGN VELPQEAFLA ILHVGKDNK
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gtgtagaatg cggcgtttct attaatacag
-070 acgttaagct cagaacagcg acttctaaaa gcctggttaa ccgggcatta aggcacaata atcatacttt
0001 atgaagaata tacgtaactt ttcgatcata gctcacattg accacggtaa atcgacgctg tctgaccgta
0071 ttatccagat ctgcggtggc ctgtctgacc gtgaaatgga ggcgcaggtt ctcgattcca tggatcttga
0141 gcgtgagcgt ggcattacca tcaaagcgca aagcgtgacg ctggactaca aagcgtctga cggcgaaacc
0211 tatcagctta actttatcga caccccgggc cacgtagact tctcctatga agtttcccgt tcgctggctg
0281 cctgtgaagg tgcattgctg gtggtcgacg ccgggcaggg cgtagaagcg caaaccctgg caaactgcta
0351 caccgccatg gaaatggatc tcgaagttgt gccggtactg aacaagattg acctgccggc agccgatcct
0421 gaacgcgtgg cggaagaaat tgaagatatc gtcggcatcg acgccaccga cgcggtgcgc tgttcagcga
0491 aaaccggcgt tggtgtgcag gacgttctcg aacgtctggt gcgcgacatt ccgccgccgg aaggcgatcc
0561 ggaaggcccg ttgcaggcac taattatcga ctcatggttc gacaactacc tgggcgttgt ttcacttatc
0631 cgtattaaaa acggcaccct gcgtaagggc gacaaagtga aagtcatgag taccgggcag acctataacg
0701 ccgaccgtct gggcatcttc acgccgaaac aggttgaccg cactgaactg aaatgtggcg aagtaggctg
0771 gctcgtatgt gcgattaaag atatccacgg cgctccagtc ggcgatacct taacgctggc gcgtaatccg
0841 gcagaaaagg cgctgcctgg ctttaagaaa gtcaaaccgc aggtatacgc cggtctgttc ccggtaagtt
0911 ccgacgacta tgaagccttc cgtgacgcgc tgggtaaact cagcctgaac gatgcctcac tgttctatga
0981 gccggaaagc tccagcgcgc tgggctttgg tttccgctgc ggcttcctcg gcctgctgca catggagatc
1051 atccaggaac gtctggaacg tgaatacgat ctggatctga tcaccactgc gccgaccgta gtgtatgaag
1121 ttgaaaccac gtcaagagaa gttatctacg tcgacagccc atccaagctg cctgcggtaa ataacatcta
1191 cgaactgcgc gagccgattg cagagtgtca catgctgctg ccgcaggcat atctcggcaa cgttattacg
1261 ttgtgcgtag aaaaacgcgg cgtgcagacc aatatggttt accacggtaa tcaggtggcg ctgacgtacg
1331 agatcccgat ggcggaagtg gtgctcgact tcttcgatcg cctgaaatct acctcgcgtg gttatgcgtc
1401 tctggattac aacttcaagc gcttccaggc gtccgacatg gtacgtgtag acgtattaat caacggtgaa
1471 cgtgttgatg cgctggcgtt gatcacccac cgtgataatt cgcaaaaccg cggtcgcgag ttggtggaga
1541 agatgaaaga tctgatccca cgccagcagt ttgatatcgc cattcaggca gcgattggta cgcacatcat
1611 tgcgcgatcc accgtgaaac agctgcgtaa aaacgtactg gctaaatgtt atggcggcga tatcagccgt
1681 aagaaaaagc tgctgcagaa gcagaaagaa ggtaagaaac gcatgaagca gatcggtaac gtcgagctgc
1751 cgcaggaagc gttcctcgcc attctgcacg tcggcaaaga caacaaataa cccttaggag ttggcatggc
1821 gaatatgttt gccctgattc tggtgattgc cacactggtg acgggcattt tatggtgcgt ggataaattc
1891 tttttcgcac
|
