MG1655
W3110
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Gene Report : lepB
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Bacteriol. 1986;166(1):83-7.
lep operon proximal gene is not required for growth or secretion by Escherichia coli.
Dibb NJ, Wolfe PB. ( 3514582 )
     Deletion OCL53,54-1 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  lepB
Related strains
     Strains Search Search strains by gene name:  lepB   Search strains by all related name:  lepB b2568 ECK2566 JW2552 lep

General information  (Go to Linear View:)
 Gene Name lepB  
 Alternative name b2568,ECK2566,JW2552,lep  
 Location, Length 2,702,357 - 2,703,331 (  -  ) ;   58.24 min ; 975 (bp) ,   324 (aa) Go to Linear View
 Product leader peptidase (signal peptidase I)  
 Operon Name lepAB  
 Note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane  
 Function enzyme; Protein, peptide secretion  
 Gene Ontology GO:0005886 ; plasma membrane ( lepB )
GO:0006508 ; proteolysis ( lepB )
GO:0008233 ; peptidase activity ( lepB )
GO:0008236 ; serine-type peptidase activity ( lepB )
GO:0009003 ; signal peptidase activity ( lepB )
GO:0009004 ; signal peptidase I activity ( lepB )
GO:0016020 ; membrane ( lepB )
GO:0016021 ; integral to membrane ( lepB )
GO:0016787 ; hydrolase activity ( lepB )
 PID 1788921  
 EC number
  (KEGG Pathway)   		3.4.21.89  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) LEP_ECOLI ( P00803 ; P78098 )
    -  Protein name Signal peptidase I  
    -  Synonyms EC 3.4.21.89, SPase I, Leader peptidase I  
    -  Gene name Name=lepB; OrderedLocusNames=b2568;  

 Linear View (Whole Mode)
View Location
   2690.0  –  2715.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP lepB (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Escherichia Coli Type I Signal Peptidase In Complex With A Lipopeptide Inhibitor  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Signal peptidase I  
         PSI-BLAST Signal peptidase I
    nr     (database updated : 2007.02.20 )
         BLAST leader peptidase (signal peptidase I) [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    PROKAR_LIPOPROTEIN    RGD    SPASE_I_1    SPASE_I_2    SPASE_I_3     

Other Cross-Reference
    COG COG0681U 
    EcoCyc lepB 

MMBR References
J Biol Chem. 1985;260:7206-7213
Characterization of the lep operon of Escherichia coli. Identification of the promoter and the gene upstream of the signal peptidase I gene.
March, P. E., and M. Inoue.
J Bacteriol. 1983;154(2):569-72.
Genetic mapping of the Escherichia coli leader (signal) peptidase gene (lep): a new approach for determining the map position of a cloned gene.
Silver P, Wickner W. ( 6341355 )
J Biol Chem. 1983;258:12073-12080
Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in the bacterial envelope.
Wolfe, P. B., W. Wickner, and J. M. Goodman.
Mol Microbiol. 1991;5(12):2855-60.
Leader peptidase.
Dalbey RE. ( 1809829 )

Sequences
Amino acid
FASTA format 
0001 MANMFALILV IATLVTGILW CVDKFFFAPK RRERQAAAQA AAGDSLDKAT LKKVAPKPGW LETGASVFPV 
0071 LAIVLIVRSF IYEPFQIPSG SMMPTLLIGD FILVEKFAYG IKDPIYQKTL IETGHPKRGD IVVFKYPEDP 
0141 KLDYIKRAVG LPGDKVTYDP VSKELTIQPG CSSGQACENA LPVTYSNVEP SDFVQTFSRR NGGEATSGFF 
0211 EVPKNETKEN GIRLSERKET LGDVTHRILT VPIAQDQVGM YYQQPGQQLA TWIVPPGQYF MMGDNRDNSA 
0281 DSRYWGFVPE ANLVGRATAI WMSFDKQEGE WPTGLRLSRI GGIH
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             gaaacgcatg aagcagatcg gtaacgtcga 
-070 gctgccgcag gaagcgttcc tcgccattct gcacgtcggc aaagacaaca aataaccctt aggagttggc 
0001 atggcgaata tgtttgccct gattctggtg attgccacac tggtgacggg cattttatgg tgcgtggata 
0071 aattcttttt cgcacctaaa cggcgggaac gtcaggcagc ggcgcaggcg gctgccgggg actcactgga 
0141 taaagcaacg ttgaaaaagg ttgcgccgaa gcctggctgg ctggaaaccg gtgcttctgt ttttccggta 
0211 ctggctatcg tattgattgt gcgttcgttt atttatgaac cgttccagat cccgtcaggt tcgatgatgc 
0281 cgactctgtt aattggtgat tttattctgg tagagaagtt tgcttatggc attaaagatc ctatctacca 
0351 gaaaacgctg atcgaaaccg gtcatccgaa acgcggcgat atcgtggtct ttaaatatcc ggaagatcca 
0421 aagcttgatt acatcaagcg cgcggtgggt ttaccgggcg ataaagtcac ttacgatccg gtctcaaaag 
0491 agctgacgat tcaaccggga tgcagttccg gccaggcgtg tgaaaacgcg ctgccggtca cctactcaaa 
0561 cgtggaaccg agcgatttcg ttcagacctt ctcacgccgt aatggtgggg aagcgaccag cggattcttt 
0631 gaagtgccga aaaacgaaac caaagaaaat ggaattcgtc tttccgagcg taaagagaca ctgggtgatg 
0701 tgacgcaccg cattctgaca gtgccgattg cgcaggatca ggtggggatg tattaccagc agccagggca 
0771 acaactggca acctggattg ttcctccggg acaatacttc atgatgggcg acaaccgcga caacagcgcg 
0841 gacagccgtt actggggctt tgtgccggaa gcgaatctgg tcggtcgggc aacggctatc tggatgagct 
0911 tcgataagca agaaggcgaa tggccgactg gtctgcgctt aagtcgcatt ggcggcatcc attaatagcc 
0981 atcttcgttc acgttgtcgc cgttatggcg acaacgtgaa ttatttatga gataaatctc ccgtggctaa 
1051 cgacatcccc cgtcgttgtg tatag