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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
|
OCL54
(D)
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|
Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
glyA
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Related strains
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Strains Search
|
Search strains by gene name:
glyA
Search strains by all related name:
glyA b2551 ECK2548 JW2535
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General information (Go to Linear View: )
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Gene Name
|
glyA
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Alternative name
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b2551,ECK2548,JW2535
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Location, Length
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2,682,276 - 2,683,529
(
-
)
;
57.81
min
;
1254
(bp) ,
417
(aa)
|
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Product
|
serine hydroxymethyltransferase
|
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Operon Name
|
glyA
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009063 - cellular amino acid catabolic process; GO_process: GO:0006545 - glycine biosynthetic process; GO_process: GO:0046656 - folic acid biosynthetic process
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Function
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enzyme; Amino acid biosynthesis: Glycine
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Gene Ontology
|
GO:0003824
;
catalytic activity ( glyA )
GO:0004372
;
glycine hydroxymethyltransferase activity ( glyA )
GO:0005624
;
membrane fraction ( glyA )
GO:0005737
;
cytoplasm ( glyA )
GO:0006544
;
glycine metabolic process ( glyA )
GO:0006563
;
L-serine metabolic process ( glyA )
GO:0006730
;
one-carbon compound metabolic process ( glyA )
GO:0009069
;
serine family amino acid metabolic process ( glyA )
GO:0016740
;
transferase activity ( glyA )
GO:0030170
;
pyridoxal phosphate binding ( glyA )
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PID
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1788902
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EC number
(KEGG Pathway)
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2.1.2.1
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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glyA
(
EG10408
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
|
GLYA_ECOLI
(
P00477
)
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- Protein name
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Serine hydroxymethyltransferase
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- Synonyms
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EC 2.1.2.1, Serine methylase, SHMT
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- Gene name
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Name=glyA; OrderedLocusNames=b2551, c3073, SF2598, S2770;
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MMBR References
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FEMS Microbiol Lett. 1996;144(2-3):229-33.
MetR-mediated repression of the glyA gene in Escherichia coli.
Lorenz E, Stauffer GV.
(
8900067
)
|
Gene. 1983;22(1):9-18.
Characterization of the Escherichia coli gene for serine hydroxymethyltransferase.
Plamann MD, Stauffer GV.
(
6190704
)
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Nucleic Acids Res. 1983;11:2065-2075
Complete nucleotide sequence of the E. coli glyA gene.
Plamann, M. D., L. T. Stauffer, M. L. Urbanowski, and G. V. Stauffer.
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Gene. 1981;14(1-2):63-72.
Construction and expression of hybrid plasmids containing the Escherichia coli glyA genes.
Stauffer GV, Plamann MD, Stauffer LT.
(
7021324
)
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Biochem Biophys Res Commun. 1976;69(3):751-8.
A rapid test for the rel A mutation in E. coli.
Uzan M, Danchin A.
(
773380
)
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| Sequences |
Amino acid
FASTA format
|
0001 MLKREMNIAD YDAELWQAME QEKVRQEEHI ELIASENYTS PRVMQAQGSQ LTNKYAEGYP GKRYYGGCEY
0071 VDIVEQLAID RAKELFGADY ANVQPHSGSQ ANFAVYTALL EPGDTVLGMN LAHGGHLTHG SPVNFSGKLY
0141 NIVPYGIDAT GHIDYADLEK QAKEHKPKMI IGGFSAYSGV VDWAKMREIA DSIGAYLFVD MAHVAGLVAA
0211 GVYPNPVPHA HVVTTTTHKT LAGPRGGLIL AKGGSEELYK KLNSAVFPGG QGGPLMHVIA GKAVALKEAM
0281 EPEFKTYQQQ VAKNAKAMVE VFLERGYKVV SGGTDNHLFL VDLVDKNLTG KEADAALGRA NITVNKNSVP
0351 NDPKSPFVTS GIRVGTPAIT RRGFKEAEAK ELAGWMCDVL DSINDEAVIE RIKGKVLDIC ARYPVYA
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ctgttatcgc acaatgattc ggttatactg
-070 ttcgccgttg tccaacagga ccgcctataa aggccaaaaa ttttattgtt agctgagtca ggagatgcgg
0001 atgttaaagc gtgaaatgaa cattgccgat tatgatgccg aactgtggca ggctatggag caggaaaaag
0071 tacgtcagga agagcacatc gaactgatcg cctccgaaaa ctacaccagc ccgcgcgtaa tgcaggcgca
0141 gggttctcag ctgaccaaca aatatgctga aggttatccg ggcaaacgct actacggcgg ttgcgagtat
0211 gttgatatcg ttgaacaact ggcgatcgat cgtgcgaaag aactgttcgg cgctgactac gctaacgtcc
0281 agccgcactc cggctcccag gctaactttg cggtctacac cgcgctgctg gaaccaggtg ataccgttct
0351 gggtatgaac ctggcgcatg gcggtcacct gactcacggt tctccggtta acttctccgg taaactgtac
0421 aacatcgttc cttacggtat cgatgctacc ggtcatatcg actacgccga tctggaaaaa caagccaaag
0491 aacacaagcc gaaaatgatt atcggtggtt tctctgcata ttccggcgtg gtggactggg cgaaaatgcg
0561 tgaaatcgct gacagcatcg gtgcttacct gttcgttgat atggcgcacg ttgcgggcct ggttgctgct
0631 ggcgtctacc cgaacccggt tcctcatgct cacgttgtta ctaccaccac tcacaaaacc ctggcgggtc
0701 cgcgcggcgg cctgatcctg gcgaaaggtg gtagcgaaga gctgtacaaa aaactgaact ctgccgtttt
0771 ccctggtggt cagggcggtc cgttgatgca cgtaatcgcc ggtaaagcgg ttgctctgaa agaagcgatg
0841 gagcctgagt tcaaaactta ccagcagcag gtcgctaaaa acgctaaagc gatggtagaa gtgttcctcg
0911 agcgcggcta caaagtggtt tccggcggca ctgataacca cctgttcctg gttgatctgg ttgataaaaa
0981 cctgaccggt aaagaagcag acgccgctct gggccgtgct aacatcaccg tcaacaaaaa cagcgtaccg
1051 aacgatccga agagcccgtt tgtgacctcc ggtattcgtg taggtactcc ggcgattacc cgtcgcggct
1121 ttaaagaagc cgaagcgaaa gaactggctg gctggatgtg tgacgtgctg gacagcatca atgatgaagc
1191 cgttatcgag cgcatcaaag gtaaagttct cgacatctgc gcacgttacc cggtttacgc ataagcgaaa
1261 cggtgatttg ctgtcaatgt gctcgttgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa
1331 actttgcaaa ttcaatatat tgca
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