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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Microbiol Mol Biol Rev. 1998;62(3):814-984
Linkage map of Escherichia coli K-12, edition 10: the traditional map.
Berlyn MK.
(
9729611
)
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Deletion
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LD3-4-1
(LD)
,
OCL59
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
narQ
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Related strains
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Strains Search
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Search strains by gene name:
narQ
Search strains by all related name:
narQ b2469 ECK2464 JW2453
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General information (Go to Linear View: )
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Gene Name
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narQ
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Alternative name
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b2469,ECK2464,JW2453
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Location, Length
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2,583,753 - 2,585,453
(
+
)
;
55.69
min
;
1701
(bp) ,
566
(aa)
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Product
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sensory histidine kinase in two-component regulatory system with NarP (NarL)
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Operon Name
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narQ
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Note
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sensor for nitrate reductase system, protein histidine kinase (acts on NarP and narL); GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration; GO_process: GO:0006464 - protein modification process
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Function
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enzyme; Energy metabolism, carbon: Anaerobic respiration
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Gene Ontology
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GO:0000155
;
two-component sensor activity ( narQ )
GO:0000160
;
two-component signal transduction system (phosphorelay) ( narQ )
GO:0004673
;
protein histidine kinase activity ( narQ )
GO:0004871
;
signal transducer activity ( narQ )
GO:0005524
;
ATP binding ( narQ )
GO:0005886
;
plasma membrane ( narQ )
GO:0007165
;
signal transduction ( narQ )
GO:0016020
;
membrane ( narQ )
GO:0016021
;
integral to membrane ( narQ )
GO:0016301
;
kinase activity ( narQ )
GO:0016740
;
transferase activity ( narQ )
GO:0018106
;
peptidyl-histidine phosphorylation ( narQ )
GO:0042128
;
nitrate assimilation ( narQ )
GO:0046983
;
protein dimerization activity ( narQ )
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PID
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1788812
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EC number
(KEGG Pathway)
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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NARQ_ECOLI
(
P27896
)
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- Protein name
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Nitrate/nitrite sensor protein narQ
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- Synonyms
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EC 2.7.3.-
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- Gene name
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Name=narQ; OrderedLocusNames=b2469;
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MMBR References
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Proc Natl Acad Sci U S A. 1992;89:8419-8423
Either of two functionally redundant sensor proteins, NarX and NarQ, is sufficient for nitrate regulation in Escherichia coli K-12.
Rabin, R. S., and V. J. Stewart.
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J Bacteriol. 1993;175:3259-3268
Dual response regulators (NarL and NarP) interact with dual sensors (NarX and NarQ) to control nitrate- and nitrite-regulated gene expression in Escherichia coli K-12.
Rabin, R. S., and V. J. Stewart.
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J Bacteriol. 1994;176:4985-4992
Phosphorylation and dephosphorylation of the NarQ, NarX, and NarL proteins of the nitrate-dependent two-component regulatory system of Escherichia coli.
Schroder, I., C. D. Wolin, R. Cavicchioli, and R. P. Gunsalus.
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Mol Microbiol. 1992;6(14):1913-23.
Identification and characterization of narQ, a second nitrate sensor for nitrate-dependent gene regulation in Escherichia coli.
Chiang RC, Cavicchioli R, Gunsalus RP.
(
1508040
)
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J Bacteriol. 1992;174(23):7882
Physical map location of the narQ gene of Escherichia coli.
Chiang RC, Cavicchioli R, Gunsalus RP.
(
1447165
)
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Mol Microbiol. 1997;24(5):1049-60.
'Locked-on' and 'locked-off' signal transduction mutations in the periplasmic domain of the Escherichia coli NarQ and NarX sensors affect nitrate- and nitrite-dependent regulation by NarL and NarP.
Chiang RC, Cavicchioli R, Gunsalus RP.
(
9220011
)
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| Sequences |
Amino acid
FASTA format
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0001 MIVKRPVSAS LARAFFYIVL LSILSTGIAL LTLASSLRDA EAINIAGSLR MQSYRLGYDL QSGSPQLNAH
0071 RQLFQQALHS PVLTNLNVWY VPEAVKTRYA HLNANWLEMN NRLSKGDLPW YQANINNYVN QIDLFVLALQ
0141 HYAERKMLLV VAISLAGGIG IFTLVFFTLR RIRHQVVAPL NQLVTASQRI EHGQFDSPPL DTNLPNELGL
0211 LAKTFNQMSS ELHKLYRSLE ASVEEKTRDL HEAKRRLEVL YQCSQALNTS QIDVHCFRHI LQIVRDNEAA
0281 EYLELNVGEN WRISEGQPNP ELPMQILPVT MQETVYGELH WQNSHVSSSE PLLNSVSSML GRGLYFNQAQ
0351 KHFQQLLLME ERATIARELH DSLAQVLSYL RIQLTLLKRS IPEDNATAQS IMADFSQALN DAYRQLRELL
0421 TTFRLTLQQA DLPSALREML DTLQNQTSAK LTLDCRLPTL ALDAQMQVHL LQIIREAVLN AMKHANASEI
0491 AVSCVTAPDG NHTVYIRDNG IGIGEPKEPE GHYGLNIMRE RAERLGGTLT FSQPSGGGTL VSISFRSAEG
0561 EESQLM
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 TATTTCGCTA TAAACTGTGG CAGATCAAAT
-070 AATCCCATCA CTGACTAAAT TGCGTTTCAG CGAACTGGAA CATTAATGAT TTTTTGTGGA GAAGACGCGT
0001 gtgattgtta aacgacccgt ctcggccagt ctggcccggg ccttttttta cattgtgctg ctgtcgattc
0071 tttccacggg tatcgctctg ctaactctgg cgagcagttt gcgcgacgct gaggctatca atattgccgg
0141 atcgctgcgt atgcagagtt accgcctggg ctacgacttg caaagtggca gtccacaact caatgcacat
0211 cgccagttat ttcagcaggc actgcattca ccggtattaa ccaacctcaa cgtctggtat gtgccagaag
0281 cagtaaaaac tcgctatgcg catctgaatg ccaactggct ggagatgaat aatcggctca gcaagggcga
0351 tttgccgtgg tatcaggcca atattaataa ttatgttaat cagatagacc tgttcgtact ggctttacag
0421 cactacgctg aacgcaaaat gctgctggtg gtggcgattt ccctggctgg cggcatcggt attttcacgc
0491 tggtcttttt tactttgcgc cgcatacgcc atcaggtggt tgccccgctg aatcagctgg ttaccgccag
0561 tcagcgtatt gaacacgggc agttcgactc gccgccgctg gataccaacc tgccgaatga gcttggtctg
0631 cttgcaaaaa cctttaacca gatgtcgagc gagctgcata aattgtaccg ttcgctggaa gcgtcagtag
0701 aagaaaagac ccgcgatctc cacgaggcca agcgtcgtct ggaggtgttg tatcagtgtt cgcaggcgct
0771 gaacactagc cagattgatg tgcattgttt ccgccatatt ttgcagattg ttcgcgacaa tgaagcggct
0841 gaatatctgg agttaaatgt cggtgaaaac tggcggatta gcgaagggca accaaacccg gaattgccga
0911 tgcagatttt accggtgaca atgcaagaga cggtttacgg cgaactgcac tggcaaaata gtcacgtttc
0981 atcatcagaa ccgctgctta acagcgtttc gtcgatgctg ggacgcggtt tgtactttaa tcaggcgcag
1051 aagcattttc agcaattatt gttgatggaa gaacgtgcga ccatcgcccg cgaattgcac gactcgctgg
1121 ctcaggtact ttcttactta cgtatccagt tgacgttact gaagcgttcg ataccggaag ataacgccac
1191 cgcacaaagt atcatggccg atttttccca ggcgttgaat gatgcttatc ggcagttacg cgagctgttg
1261 actactttcc gcctgacgct gcagcaggcg gatctcccct ccgcattgag ggaaatgctg gatacgttac
1331 aaaatcaaac cagcgccaaa ctgaccctcg actgccgtct gccaaccctg gcactggatg cgcaaatgca
1401 ggtgcatttg ttgcaaatta ttcgcgaagc ggtgctgaat gcgatgaagc acgccaacgc cagcgaaatc
1471 gccgtcagtt gcgtcaccgc gccggacggc aatcacacgg tttatatccg tgataacggg attggtatcg
1541 gtgaaccgaa agaacccgaa ggtcattatg gtctgaatat catgcgcgaa cgcgcggaac ggctaggtgg
1611 gacgctgact ttttcacaac cttccggcgg cggcacgtta gtgagtatta gctttcgctc tgcggagggt
1681 gaggaaagtc agttaatgta aTGCCTCCTA CTGACCAAAG AATACTTGCA CTTAAGGTTC AGTATAAAAG
1751 GGCATGATAA TTTACATTAA CTCCTTTTTT TCTCCACGAT TGGCTCGTAC C
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