MG1655
W3110
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Gene Report : proA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion DE(gpt-lac)5  ,  OCR36,37-1 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  proA
Related strains
     Strains Search Search strains by gene name:  proA   Search strains by all related name:  proA b0243 ECK0244 JW0233 pro pro-1

General information  (Go to Linear View:)
 Gene Name proA  
 Alternative name b0243,ECK0244,JW0233,pro,pro-1  
 Location, Length 260,727 - 261,980 (  +  ) ;   5.62 min ; 1254 (bp) ,   417 (aa) Go to Linear View
 Product gamma-glutamylphosphate reductase  
 Operon Name proBA  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006561 - proline biosynthetic process  
 Function enzyme; Amino acid biosynthesis: Proline  
 Gene Ontology GO:0004350; glutamate-5-semialdehyde dehydrogenase activity ( proA )
GO:0005737; cytoplasm ( proA )
GO:0006561; proline biosynthetic process ( proA )
GO:0008152; metabolic process ( proA )
GO:0008652; amino acid biosynthetic process ( proA )
GO:0016491; oxidoreductase activity ( proA )
GO:0050661; NADP binding ( proA )
 PID 1786438  
 EC number
  (KEGG Pathway)
 
1.2.1.41  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene proA ( EG10767 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) PROA_ECOLI ( P07004 ; P77428 )
    -  Protein name Gamma-glutamyl phosphate reductase  
    -  Synonyms GPR, EC 1.2.1.41, Glutamate-5- semialdehyde dehydrogenase, Glutamyl-gamma-semialdehyde dehydrogenase, GSA dehydrogenase  
    -  Gene name Name=proA; OrderedLocusNames=b0243;  

 Linear View (Whole Mode)
View Location
   250.0  –  275.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP proA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Human Mitochondrial Aldehyde Dehydrogenase Complexed With Nad+ And Mn2+  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Gamma-glutamyl phosphate reductase (GPR) (Glutamate-5-semialdehyde dehydrogenase) (Glutamyl-gamma-semialdehyde dehydrogenase) (GSA dehydrogenase)  
         PSI-BLAST Gamma-glutamyl phosphate reductase
    nr     (database updated : 2007.02.20 )
         BLAST gamma-glutamyl phosphate reductase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    PROA     

Other Cross-Reference
    COG COG0014E 
    EcoCyc proA 

MMBR References
Nucleic Acids Res. 1984;12:6337-6355
Analysis of the Escherichia coli proBA locus by DNA and protein sequencing.
Deutch, A. H., K. E. Rushlow, and C. J. Smith.
J Biol Chem. 1979;254:9429-9440
ilvU, a locus in Escherichia coli affecting the derepression of isoleucyl-tRNA synthetase in the RPC-5 chromatographic profiles of tRNAIle and tRNAVal.
Fayerman, J. T., M. C. Vann, L. S. Williams, and H. E. Umbarger.
Gene. 1983;22(2-3):281-7.
A partial restriction map of the proA-purE region of the Escherichia coli K12 chromosome.
Hadley RG, Hu M, Timmons M, Yun K, Deonier RC. ( 6307829 )
J Gen Microbiol. 1983;129(10):3215-25.
Sub-cloning of the wild-type proAB region of the Escherichia coli genome.
Hayzer DJ. ( 6317797 )
J Gen Microbiol. 1980;118(2):287-93.
The gene-enzyme relationships of proline biosynthesis in Escherichia coli.
Hayzer DJ, Leisinger T. ( 6255065 )
J Bacteriol. 1991;173(23):7407-8.
Locations of the genes from pepD through proA on the physical map of the Escherichia coli chromosome.
Henrich B, Plapp R. ( 1938933 )
J Bacteriol. 1966;91(6):2392
Elective selection of proline-requiring mutants.
Hirota Y, Inuzuka M, Tomoeda M. ( 5329291 )
J Bacteriol. 1983;156(3):1249-62.
Genetic analysis of the proBA genes of Salmonella typhimurium: physical and genetic analyses of the cloned proB+ A+ genes of Escherichia coli and of a mutant allele that confers proline overproduction and enhanced osmotolerance.
Mahan MJ, Csonka LN. ( 6315682 )
J Bacteriol. 1974;118(3):928-34.
Proline excretion and indirect suppression in Escherichia coli and Salmonella typhimurium.
Berg CM, Rossi JJ. ( 4598010 )
Sov. Genet. (Engl. Transl. Genetika) 1986;22:1493-1500.
Cloning of Escherichia coli genes for proline biosynthesis.
Nersisyan, A. A., Y. A. Fedorova, and E. M. Khurges.
Mol Gen Genet. 1976;147(3):263-9.
Heptose-deficient mutants of Escherichia coli K12 deficient in up to three major outer membrane proteins.
van Alphen W, Lugtenberg B, Berendsen W. ( 787763 )

Sequences
Amino acid
FASTA format
0001 MLEQMGIAAK QASYKLAQLS SREKNRVLEK IADELEAQSE IILNANAQDV ADARANGLSE AMLDRLALTP 
0071 ARLKGIADDV RQVCNLADPV GQVIDGGVLD SGLRLERRRV PLGVIGVIYE ARPNVTVDVA SLCLKTGNAV 
0141 ILRGGKETCR TNAATVAVIQ DALKSCGLPA GAVQAIDNPD RALVSEMLRM DKYIDMLIPR GGAGLHKLCR 
0211 EQSTIPVITG GIGVCHIYVD ESVEIAEALK VIVNAKTQRP STCNTVETLL VNKNIADSFL PALSKQMAES 
0281 GVTLHADAAA LAQLQAGPAK VVAVKAEEYD DEFLSLDLNV KIVSDLDDAI AHIREHGTQH SDAILTRDMR 
0351 NAQRFVNEVD SSAVYVNAST RFTDGGQFGL GAEVAVSTQK LHARGPMGLE ALTTYKWIGI GDYTIRA

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             CCGGACACCA CTCGCAAGAA ATTGATGCAA 
-070 TACTGGGATA TGAATACGGC CCGGTTGCCG TTCACCGTGA TGACATGATT ACCCGTTAAG GAGCAGGCTG 
0001 atgctggaac aaatgggcat tgccgcgaag caagcctcgt ataaattagc gcaactctcc agccgcgaaa 
0071 aaaatcgcgt gctggaaaaa atcgccgatg aactggaagc acaaagcgaa atcatcctca acgctaacgc 
0141 ccaggatgtt gctgacgcgc gagccaatgg ccttagcgaa gcgatgcttg accgtctggc actgacgccc 
0211 gcacggctga aaggcattgc cgacgatgta cgtcaggtgt gcaacctcgc cgatccggtg gggcaggtaa 
0281 tcgatggcgg cgtactggac agcggcctgc gtcttgagcg tcgtcgcgta ccgctggggg ttattggcgt 
0351 gatttatgaa gcgcgcccga acgtgacggt tgatgtcgct tcgctgtgcc tgaaaaccgg taatgcggtg 
0421 atcctgcgcg gtggcaaaga aacgtgtcgc actaacgctg caacggtggc ggtgattcag gacgccctga 
0491 aatcctgcgg cttaccggcg ggtgccgtgc aggcgattga taatcctgac cgtgcgctgg tcagtgaaat 
0561 gctgcgtatg gataaataca tcgacatgct gatcccgcgt ggtggcgctg gtttgcataa actgtgccgt 
0631 gaacagtcga caatcccggt gatcacaggt ggtataggcg tatgccatat ttacgttgat gaaagtgtag 
0701 agatcgctga agcattaaaa gtgatcgtca acgcgaaaac tcagcgtccg agcacatgta atacggttga 
0771 aacgttgctg gtgaataaaa acatcgccga tagcttcctg cccgcattaa gcaaacaaat ggcggaaagc 
0841 ggcgtgacat tacacgcaga tgcagctgca ctggcgcagt tgcaggcagg ccctgcgaag gtggttgctg 
0911 ttaaagccga agagtatgac gatgagtttc tgtcattaga tttgaacgtc aaaatcgtca gcgatcttga 
0981 cgatgccatc gcccatattc gtgaacacgg cacacaacac tccgatgcga tcctgacccg cgatatgcgc 
1051 aacgcccagc gttttgttaa cgaagtggat tcgtccgctg tttacgttaa cgcctctacg cgttttaccg 
1121 acggcggcca gtttggtctg ggtgcggaag tggcggtaag cacacaaaaa ctccacgcgc gtggcccaat 
1191 ggggctggaa gcactgacca cttacaagtg gatcggcatt ggtgattaca ccattcgtgc gtaaATAAAA 
1261 CCGGGTGATG CAAAAGTAGC CATTTGATTC ACAAGGCCAT TGACGCATCG CCCGGTTAGT TTTAACCTTG 
1331 TCCACCGTGA TTCACGTTCG TGAA