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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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DE(gpt-lac)5
,
OCR36,37-1
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
proA
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Related strains
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Strains Search
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Search strains by gene name:
proA
Search strains by all related name:
proA b0243 ECK0244 JW0233 pro pro-1
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General information (Go to Linear View: )
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Gene Name
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proA
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Alternative name
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b0243,ECK0244,JW0233,pro,pro-1
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Location, Length
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260,727 - 261,980
(
+
)
;
5.62
min
;
1254
(bp) ,
417
(aa)
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Product
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gamma-glutamylphosphate reductase
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Operon Name
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proBA
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006561 - proline biosynthetic process
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Function
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enzyme; Amino acid biosynthesis: Proline
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Gene Ontology
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GO:0004350;
glutamate-5-semialdehyde dehydrogenase activity ( proA )
GO:0005737;
cytoplasm ( proA )
GO:0006561;
proline biosynthetic process ( proA )
GO:0008152;
metabolic process ( proA )
GO:0008652;
amino acid biosynthetic process ( proA )
GO:0016491;
oxidoreductase activity ( proA )
GO:0050661;
NADP binding ( proA )
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PID
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1786438
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EC number
(KEGG Pathway)
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1.2.1.41
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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proA
(
EG10767
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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PROA_ECOLI
(
P07004
;
P77428
)
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- Protein name
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Gamma-glutamyl phosphate reductase
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- Synonyms
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GPR, EC 1.2.1.41, Glutamate-5- semialdehyde dehydrogenase, Glutamyl-gamma-semialdehyde dehydrogenase, GSA dehydrogenase
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- Gene name
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Name=proA; OrderedLocusNames=b0243;
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MMBR References
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Nucleic Acids Res. 1984;12:6337-6355
Analysis of the Escherichia coli proBA locus by DNA and protein sequencing.
Deutch, A. H., K. E. Rushlow, and C. J. Smith.
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J Biol Chem. 1979;254:9429-9440
ilvU, a locus in Escherichia coli affecting the derepression of isoleucyl-tRNA synthetase in the RPC-5 chromatographic profiles of tRNAIle and tRNAVal.
Fayerman, J. T., M. C. Vann, L. S. Williams, and H. E. Umbarger.
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Gene. 1983;22(2-3):281-7.
A partial restriction map of the proA-purE region of the Escherichia coli K12 chromosome.
Hadley RG, Hu M, Timmons M, Yun K, Deonier RC.
(
6307829
)
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J Gen Microbiol. 1983;129(10):3215-25.
Sub-cloning of the wild-type proAB region of the Escherichia coli genome.
Hayzer DJ.
(
6317797
)
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J Gen Microbiol. 1980;118(2):287-93.
The gene-enzyme relationships of proline biosynthesis in Escherichia coli.
Hayzer DJ, Leisinger T.
(
6255065
)
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J Bacteriol. 1991;173(23):7407-8.
Locations of the genes from pepD through proA on the physical map of the Escherichia coli chromosome.
Henrich B, Plapp R.
(
1938933
)
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J Bacteriol. 1966;91(6):2392
Elective selection of proline-requiring mutants.
Hirota Y, Inuzuka M, Tomoeda M.
(
5329291
)
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J Bacteriol. 1983;156(3):1249-62.
Genetic analysis of the proBA genes of Salmonella typhimurium: physical and genetic analyses of the cloned proB+ A+ genes of Escherichia coli and of a mutant allele that confers proline overproduction and enhanced osmotolerance.
Mahan MJ, Csonka LN.
(
6315682
)
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J Bacteriol. 1974;118(3):928-34.
Proline excretion and indirect suppression in Escherichia coli and Salmonella typhimurium.
Berg CM, Rossi JJ.
(
4598010
)
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Sov. Genet. (Engl. Transl. Genetika) 1986;22:1493-1500.
Cloning of Escherichia coli genes for proline biosynthesis.
Nersisyan, A. A., Y. A. Fedorova, and E. M. Khurges.
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Mol Gen Genet. 1976;147(3):263-9.
Heptose-deficient mutants of Escherichia coli K12 deficient in up to three major outer membrane proteins.
van Alphen W, Lugtenberg B, Berendsen W.
(
787763
)
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| Sequences |
Amino acid
FASTA format
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0001 MLEQMGIAAK QASYKLAQLS SREKNRVLEK IADELEAQSE IILNANAQDV ADARANGLSE AMLDRLALTP
0071 ARLKGIADDV RQVCNLADPV GQVIDGGVLD SGLRLERRRV PLGVIGVIYE ARPNVTVDVA SLCLKTGNAV
0141 ILRGGKETCR TNAATVAVIQ DALKSCGLPA GAVQAIDNPD RALVSEMLRM DKYIDMLIPR GGAGLHKLCR
0211 EQSTIPVITG GIGVCHIYVD ESVEIAEALK VIVNAKTQRP STCNTVETLL VNKNIADSFL PALSKQMAES
0281 GVTLHADAAA LAQLQAGPAK VVAVKAEEYD DEFLSLDLNV KIVSDLDDAI AHIREHGTQH SDAILTRDMR
0351 NAQRFVNEVD SSAVYVNAST RFTDGGQFGL GAEVAVSTQK LHARGPMGLE ALTTYKWIGI GDYTIRA
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CCGGACACCA CTCGCAAGAA ATTGATGCAA
-070 TACTGGGATA TGAATACGGC CCGGTTGCCG TTCACCGTGA TGACATGATT ACCCGTTAAG GAGCAGGCTG
0001 atgctggaac aaatgggcat tgccgcgaag caagcctcgt ataaattagc gcaactctcc agccgcgaaa
0071 aaaatcgcgt gctggaaaaa atcgccgatg aactggaagc acaaagcgaa atcatcctca acgctaacgc
0141 ccaggatgtt gctgacgcgc gagccaatgg ccttagcgaa gcgatgcttg accgtctggc actgacgccc
0211 gcacggctga aaggcattgc cgacgatgta cgtcaggtgt gcaacctcgc cgatccggtg gggcaggtaa
0281 tcgatggcgg cgtactggac agcggcctgc gtcttgagcg tcgtcgcgta ccgctggggg ttattggcgt
0351 gatttatgaa gcgcgcccga acgtgacggt tgatgtcgct tcgctgtgcc tgaaaaccgg taatgcggtg
0421 atcctgcgcg gtggcaaaga aacgtgtcgc actaacgctg caacggtggc ggtgattcag gacgccctga
0491 aatcctgcgg cttaccggcg ggtgccgtgc aggcgattga taatcctgac cgtgcgctgg tcagtgaaat
0561 gctgcgtatg gataaataca tcgacatgct gatcccgcgt ggtggcgctg gtttgcataa actgtgccgt
0631 gaacagtcga caatcccggt gatcacaggt ggtataggcg tatgccatat ttacgttgat gaaagtgtag
0701 agatcgctga agcattaaaa gtgatcgtca acgcgaaaac tcagcgtccg agcacatgta atacggttga
0771 aacgttgctg gtgaataaaa acatcgccga tagcttcctg cccgcattaa gcaaacaaat ggcggaaagc
0841 ggcgtgacat tacacgcaga tgcagctgca ctggcgcagt tgcaggcagg ccctgcgaag gtggttgctg
0911 ttaaagccga agagtatgac gatgagtttc tgtcattaga tttgaacgtc aaaatcgtca gcgatcttga
0981 cgatgccatc gcccatattc gtgaacacgg cacacaacac tccgatgcga tcctgacccg cgatatgcgc
1051 aacgcccagc gttttgttaa cgaagtggat tcgtccgctg tttacgttaa cgcctctacg cgttttaccg
1121 acggcggcca gtttggtctg ggtgcggaag tggcggtaag cacacaaaaa ctccacgcgc gtggcccaat
1191 ggggctggaa gcactgacca cttacaagtg gatcggcatt ggtgattaca ccattcgtgc gtaaATAAAA
1261 CCGGGTGATG CAAAAGTAGC CATTTGATTC ACAAGGCCAT TGACGCATCG CCCGGTTAGT TTTAACCTTG
1331 TCCACCGTGA TTCACGTTCG TGAA
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