MG1655
W3110
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Gene Report : proB
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion DE(gpt-lac)5  ,  OCR36,37-1 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  proB
Related strains
     Strains Search Search strains by gene name:  proB   Search strains by all related name:  proB b0242 ECK0243 JW0232 pro pro-2

General information  (Go to Linear View:)
 Gene Name proB  
 Alternative name b0242,ECK0243,JW0232,pro,pro-2  
 Location, Length 259,612 - 260,715 (  +  ) ;   5.60 min ; 1104 (bp) ,   367 (aa) Go to Linear View
 Product gamma-glutamate kinase  
 Operon Name proBA  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006561 - proline biosynthetic process  
 Function enzyme; Amino acid biosynthesis: Proline  
 Gene Ontology GO:0003723; RNA binding ( proB )
GO:0004349; glutamate 5-kinase activity ( proB )
GO:0005737; cytoplasm ( proB )
GO:0006561; proline biosynthetic process ( proB )
GO:0008652; amino acid biosynthetic process ( proB )
GO:0016301; kinase activity ( proB )
GO:0016740; transferase activity ( proB )
 PID 1786437  
 EC number
  (KEGG Pathway)
 
2.7.2.11  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene proB ( EG10768 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) PROB_ECOLI ( P07005 ; P78293 )
    -  Protein name Glutamate 5-kinase  
    -  Synonyms EC 2.7.2.11, Gamma-glutamyl kinase, GK  
    -  Gene name Name=proB; OrderedLocusNames=b0242, c0389, z0303, ECs0269;  

 Linear View (Whole Mode)
View Location
   250.0  –  275.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP proB (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Glutamate 5-Kinase From Campylobacter Jejuni  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Glutamate 5-kinase (Gamma-glutamyl kinase) (GK)  
         PSI-BLAST Glutamate 5-kinase
    nr     (database updated : 2007.02.20 )
         BLAST gamma-glutamyl kinase [Escherichia coli CFT073]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    GLUTAMATE_5_KINASE     

Other Cross-Reference
    COG COG0263E 
    EcoCyc proB 

MMBR References
Nucleic Acids Res. 1984;12:6337-6355
Analysis of the Escherichia coli proBA locus by DNA and protein sequencing.
Deutch, A. H., K. E. Rushlow, and C. J. Smith.
J Biol Chem. 1979;254:9429-9440
ilvU, a locus in Escherichia coli affecting the derepression of isoleucyl-tRNA synthetase in the RPC-5 chromatographic profiles of tRNAIle and tRNAVal.
Fayerman, J. T., M. C. Vann, L. S. Williams, and H. E. Umbarger.
Gene. 1983;22(2-3):281-7.
A partial restriction map of the proA-purE region of the Escherichia coli K12 chromosome.
Hadley RG, Hu M, Timmons M, Yun K, Deonier RC. ( 6307829 )
J Gen Microbiol. 1983;129(10):3215-25.
Sub-cloning of the wild-type proAB region of the Escherichia coli genome.
Hayzer DJ. ( 6317797 )
J Gen Microbiol. 1980;118(2):287-93.
The gene-enzyme relationships of proline biosynthesis in Escherichia coli.
Hayzer DJ, Leisinger T. ( 6255065 )
J Bacteriol. 1966;91(6):2392
Elective selection of proline-requiring mutants.
Hirota Y, Inuzuka M, Tomoeda M. ( 5329291 )
J Bacteriol. 1983;156(3):1249-62.
Genetic analysis of the proBA genes of Salmonella typhimurium: physical and genetic analyses of the cloned proB+ A+ genes of Escherichia coli and of a mutant allele that confers proline overproduction and enhanced osmotolerance.
Mahan MJ, Csonka LN. ( 6315682 )
J Bacteriol. 1974;118(3):928-34.
Proline excretion and indirect suppression in Escherichia coli and Salmonella typhimurium.
Berg CM, Rossi JJ. ( 4598010 )
Sov. Genet. (Engl. Transl. Genetika) 1986;22:1493-1500.
Cloning of Escherichia coli genes for proline biosynthesis.
Nersisyan, A. A., Y. A. Fedorova, and E. M. Khurges.
Biotechnol. Bioeng. 1984;26:74-80.
Proline excretion by Escherichia coli K12.
Rancourt, D. E., J. T. Stephenson, G. A. Vickell, and J. M. Wood.
Mol Gen Genet. 1976;147(3):263-9.
Heptose-deficient mutants of Escherichia coli K12 deficient in up to three major outer membrane proteins.
van Alphen W, Lugtenberg B, Berendsen W. ( 787763 )
Mol Gen Genet. 1981;182(1):82-6.
Proline over-production results in enhanced osmotolerance in Salmonella typhimurium.
Csonka LN. ( 7022140 )

Sequences
Amino acid
FASTA format
0001 MSDSQTLVVK LGTSVLTGGS RRLNRAHIVE LVRQCAQLHA AGHRIVIVTS GAIAAGREHL GYPELPATIA 
0071 SKQLLAAVGQ SRLIQLWEQL FSIYGIHVGQ MLLTRADMED RERFLNARDT LRALLDNNIV PVINENDAVA 
0141 TAEIKVGDND NLSALAAILA GADKLLLLTD QKGLYTADPR SNPQAELIKD VYGIDDALRA IAGDSVSGLG 
0211 TGGMSTKLQA ADVACRAGID TIIAAGSKPG VIGDVMEGIS VGTLFHAQAT PLENRKRWIF GAPPAGEITV 
0281 DEGATAAILE RGSSLLPKGI KSVTGNFSRG EVIRICNLEG RDIAHGVSRY NSDALRRIAG HHSQEIDAIL 
0351 GYEYGPVAVH RDDMITR

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             AAAACGCCAT GCTTTGCTCG CAGATGGTTG 
-070 GCAACCGACG ACAGTCCTGC TAAAACGTTC GTTTGATATC ATTTTTCCTA AAATTGAATG GCAGAGAATC 
0001 atgagtgaca gccagacgct ggtggtaaaa ctcggcacca gtgtgctaac aggcggatcg cgccgtctga 
0071 accgtgccca tatcgttgaa cttgttcgcc agtgcgcgca gttacatgcc gccgggcatc ggattgttat 
0141 tgtgacgtcg ggcgcgatcg ccgccggacg tgagcacctg ggttacccgg aactgccagc gaccatcgcc 
0211 tcgaaacaac tgctggcggc ggtagggcag agtcgactga ttcaactgtg ggaacagctg ttttcgattt 
0281 atggcattca cgtcgggcaa atgctgctga cccgtgctga tatggaagac cgtgaacgct tcctgaacgc 
0351 ccgcgacacc ctgcgagcgt tgctcgataa caatatcgtt ccggtaatca atgagaacga tgctgtcgct 
0421 acggcagaga ttaaggtcgg cgataacgat aacctttctg cgctggcggc gattcttgcg ggtgccgata 
0491 aactgttgct gctgaccgat caaaaaggtt tgtataccgc tgacccgcgc agcaatccgc aggcagaact 
0561 gattaaagat gtttacggca ttgatgacgc actgcgcgcg attgccggtg acagcgtttc aggcctcgga 
0631 actggcggca tgagtaccaa attgcaggcc gctgacgtgg cttgccgtgc gggtatcgac accattattg 
0701 ccgcgggcag caagccgggc gttattggtg atgtgatgga aggcatttcc gtcggtacgc tgttccatgc 
0771 ccaggcgact ccgcttgaaa accgtaaacg ctggattttc ggtgcgccgc cggcgggtga aatcacggta 
0841 gatgaagggg caactgccgc cattctggaa cgcggcagct ccctgttgcc gaaaggcatt aaaagcgtga 
0911 ctggcaattt ctcgcgtggt gaagtcatcc gcatttgcaa cctcgaaggc cgcgatatcg cccacggcgt 
0981 cagtcgttac aacagcgatg cattacgccg tattgccgga caccactcgc aagaaattga tgcaatactg 
1051 ggatatgaat acggcccggt tgccgttcac cgtgatgaca tgattacccg ttaaGGAGCA GGCTGATGCT 
1121 GGAACAAATG GGCATTGCCG CGAAGCAAGC CTCGTATAAA TTAGCGCAAC TCTCCAGCCG CGAAAAAAAT 
1191 CGCGTGCTGG AAAA