|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
J Bacteriol. 1995;177(11):3326-31.
Cloning and characterization of the Escherichia coli hemN gene encoding the oxygen-independent coproporphyrinogen III oxidase.
Troup B, Hungerer C, Jahn D.
(
7768836
)
|
|
|
Deletion
|
OCL60-17
(D)
,
OCL60-18
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
hemF
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
hemF
Search strains by all related name:
hemF b2436 ECK2431 JW2429 popB sec
|
General information (Go to Linear View: )
|
|
Gene Name
|
hemF
|
|
Alternative name
|
b2436,ECK2431,JW2429,popB,sec
|
|
Location, Length
|
2,551,247 - 2,552,146
(
+
)
;
54.99
min
;
900
(bp) ,
299
(aa)
|
|
Product
|
coproporphyrinogen III oxidase
|
|
Operon Name
|
amiA-hemF
|
|
Note
|
GO_component: GO:0005737 - cytoplasm
|
|
Function
|
enzyme; Biosynthesis of cofactors, carriers: Heme, porphyrin
|
|
Gene Ontology
|
GO:0004109
;
coproporphyrinogen oxidase activity ( hemF )
GO:0005515
;
protein binding ( hemF )
GO:0005737
;
cytoplasm ( hemF )
GO:0006779
;
porphyrin biosynthetic process ( hemF )
GO:0016491
;
oxidoreductase activity ( hemF )
GO:0030145
;
manganese ion binding ( hemF )
GO:0046872
;
metal ion binding ( hemF )
|
|
PID
|
1788777
|
EC number
(KEGG Pathway)
|
1.3.3.3
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
HEM6_ECOLI
(
P36553
)
|
|
- Protein name
|
Coproporphyrinogen III oxidase, aerobic
|
|
- Synonyms
|
EC 1.3.3.3, Coproporphyrinogenase, Coprogen oxidase
|
|
- Gene name
|
Name=hemF; OrderedLocusNames=b2436;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
hemF
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Yeast Oxygen-Dependent Coproporphyrinogen Oxidase
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Coproporphyrinogen 3 oxidase, aerobic
|
|
PSI-BLAST
|
Coproporphyrinogen 3 oxidase, aerobic
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
coproporphyrinogen III oxidase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
COPROGEN_OXIDASE
|
|
MMBR References
|
J Gen Microbiol. 1979;111(1):193-200.
Mutants of Escherichia coli K12 accumulating porphobilinogen: a new locus, hemC.
McConville ML, Charles HP.
(
379277
)
|
J Bacteriol. 1997;179:330-338
Identification and characterization of hydrogen peroxide-sensitive mutants of Escherichia coli: genes that require OxyR for expression.
Mukhopadhyay, S., and H. E. Schellhorn.
|
J Bacteriol. 1994;176:673-680
Isolation of the hemF operon containing the gene for the Escherichia coli aerobic coproporphyrinogen III oxidase by in vivo complementation of a yeast HEM13 mutant.
Troup, B., M. Jahn, C. Hungerer, and D. Jahn.
|
| Sequences |
Amino acid
FASTA format
|
0001 MKPDAHQVKQ FLLNLQDTIC QQLTAVDGAE FVEDSWQREA GGGGRSRVLR NGGVFEQAGV NFSHVHGEAM
0071 PASATAHRPE LAGRSFEAMG VSLVVHPHNP YVPTSHANVR FFIAEKPGAD PVWWFGGGFD LTPFYGFEED
0141 AIHWHRTARD LCLPFGEDVY PRYKKWCDEY FYLKHRNEQR GIGGLFFDDL NTPDFDRCFA FMQAVGKGYT
0211 DAYLPIVERR KAMAYGERER NFQLYRRGRY VEFNLVWDRG TLFGLQTGGR TESILMSMPP LVRWEYDYQP
0281 KDGSPEAALS EFIKVRDWV
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GGCGTTTCGT CAGAAAATCG CCACAGCGAT
-070 TGCTGAAGGC GTGATCAGTT ATTTCCACTG GTTCGACAAC CAGAAAGCAC ATTCGAAAAA GCGATAAGTT
0001 atgaaacccg acgcacacca ggttaaacag tttctgctca accttcagga tacgatttgt cagcagctga
0071 ccgccgtcga tggcgcagaa tttgtcgaag atagttggca gcgcgaagct ggcggcggcg ggcgtagtcg
0141 ggtgttgcgt aatggtggtg ttttcgaaca ggcaggcgtc aacttttcgc atgtccacgg tgaggcgatg
0211 cctgcttccg ccaccgctca tcgcccggaa cttgccgggc gcagtttcga ggcgatgggc gtttcactgg
0281 tagtgcatcc gcataacccg tatgttccca ccagccacgc gaatgtgcgg ttttttattg ccgaaaaacc
0351 gggtgccgat cccgtctggt ggtttggcgg tggcttcgac ttaaccccat tctatggttt tgaagaagat
0421 gctattcact ggcatcgcac cgcccgtgac ctgtgcctgc catttggcga agacgtttat ccccgttaca
0491 aaaagtggtg cgacgaatac ttctacctca aacatcgcaa cgaacagcgc ggtattggcg ggctgttctt
0561 tgatgacctg aacacgccag atttcgaccg ctgttttgcc tttatgcagg cggtaggcaa aggctacacc
0631 gacgcttatt taccaattgt cgagcgacgg aaagcgatgg cctacggcga gcgcgagcgc aatttccagt
0701 tatatcgtcg cggtcgttat gtcgagttca atctggtctg ggatcgcggc acgctgtttg gcctgcaaac
0771 tggcgggcgc accgagtcta tcctgatgtc aatgccgcca ctggtacgct gggaatatga ttatcagcca
0841 aaagatggca gcccagaagc ggcgttaagt gagtttatta aggtcaggga ttgggtgtaa CTCCCTCACC
0911 CCCACTCCCG CATCCGCTGA TGCAGCGTCA GTGACGGCTT CTCGGAAAAC AGCTGCTGGT AATCCGTGGC
0981 AAATTGCCCC AGATGCCAGA
|
