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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Proc Natl Acad Sci U S A. 1990;87(1):360-3.
Mutations in the bglY gene increase the frequency of spontaneous deletions in Escherichia coli K-12.
Lejeune P, Danchin A.
(
2404280
)
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Deletion
|
OCL60-6
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
ptsI
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Related strains
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Strains Search
|
Search strains by gene name:
ptsI
Search strains by all related name:
ptsI b2416 ctr ECK2411 JW2409
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General information (Go to Linear View: )
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Gene Name
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ptsI
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Alternative name
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b2416,ctr,ECK2411,JW2409
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Location, Length
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2,532,088 - 2,533,815
(
+
)
;
54.57
min
;
1728
(bp) ,
575
(aa)
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Product
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PEP-protein phosphotransferase of PTS system (enzyme I)
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Operon Name
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ptsHI-crr
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Note
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PEP-protein phosphotransferase system enzyme I; GO_process: GO:0016052 - carbohydrate catabolic process
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Function
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enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols
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Gene Ontology
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GO:0003824
;
catalytic activity ( ptsI )
GO:0005351
;
sugar:hydrogen ion symporter activity ( ptsI )
GO:0005515
;
protein binding ( ptsI )
GO:0005737
;
cytoplasm ( ptsI )
GO:0006810
;
transport ( ptsI )
GO:0008643
;
carbohydrate transport ( ptsI )
GO:0008965
;
phosphoenolpyruvate-protein phosphotransferase activity ( ptsI )
GO:0009401
;
phosphoenolpyruvate-dependent sugar phosphotransferase system ( ptsI )
GO:0016301
;
kinase activity ( ptsI )
GO:0016310
;
phosphorylation ( ptsI )
GO:0016740
;
transferase activity ( ptsI )
GO:0016772
;
transferase activity, transferring phosphorus-containing groups ( ptsI )
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PID
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1788756
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EC number
(KEGG Pathway)
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2.7.3.9
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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ptsI
(
EG10789
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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PT1_ECOLI
(
P08839
)
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- Protein name
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Phosphoenolpyruvate-protein phosphotransferase
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- Synonyms
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EC 2.7.3.9, Phosphotransferase system, enzyme I
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- Gene name
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Name=ptsI; OrderedLocusNames=b2416;
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MMBR References
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J Bacteriol. 1970;104(2):793-7.
Isolation and mapping of phosphotransferase mutants in Escherichia coli.
Epstein W, Jewett S, Fox CF.
(
4923073
)
|
J Bacteriol. 1971;108(3):1210-23.
Resistance of Escherichia coli to penicillins. IX. Genetics and physiology of class II ampicillin-resistant mutants that are galactose negative or sensitive to bacteriophage C21, or both.
Eriksson-Grennberg KR, Nordstrom K, Englund P.
(
4945191
)
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Mol Gen Genet. 1980;179(1):49-54.
Characterisation of mutants of Escherichia coli K12, selected by resistance to streptozotocin.
Lengeler J.
(
6450313
)
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J Gen Microbiol. 1983;129(2):337-48.
Phosphotransferase-mediated regulation of carbohydrate utilization in Escherichia coli K12: the nature of the iex (crr) and gsr (tgs) mutations.
Parra F, Jones-Mortimer MC, Kornberg HL.
(
6302201
)
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J Biol Chem. 1987;262:16241-16253
Sugar transport by the bacterial phosphotransferase system. Molecular cloning and structural analysis of the Escherichia coli ptsH, ptsI and crr genes.
Saffen, D. W., K. A. Presper, T. L. Doering, and S. Roseman.
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J Bacteriol. 1972;110(3):935-44.
Isolation and characterization of a phosphonomycin-resistant mutant of Escherichia coli K-12.
Venkateswaran PS, Wu HC.
(
4555418
)
|
J Gen Microbiol. 1983;129(2):349-56.
Phosphotransferase-mediated regulation of carbohydrate utilization in Escherichia coli K12: location of the gsr (tgs) and iex (crr) genes by specialized transduction.
Britton P, Boronat A, Hartley DA, Jones-Mortimer MC, Kornberg HL, Parra F.
(
6302202
)
|
EMBO J. 1982;1(8):907-11.
Phosphotransferase-mediated regulation of carbohydrate utilisation in Escherichia coli K12: identification of the products of genes on the specialised transducing phages lambda iex (crr) and lambda gsr (tgs).
Britton P, Murfitt D, Parra F, Jones-Mortimer MC, Kornberg HL.
(
6234165
)
|
J Gen Microbiol. 1984;130(4):861-8.
Location and direction of transcription of the ptsH and ptsI genes on the Escherichia coli K12 genome.
Britton P, Lee LG, Murfitt D, Boronat A, Jones-Mortimer MC, Kornberg HL.
(
6330287
)
|
Antimicrob Agents Chemother. 1979;16(6):801-7.
Phosphorylation of streptozotocin during uptake via the phosphoenolpyruvate: sugar phosphotransferase system in Escherichia coli.
Ammer J, Brennenstuhl M, Schindler P, Holtje JV, Zahner H.
(
161156
)
|
J Bacteriol. 1988;170(9):3827-37.
The ptsH, ptsI, and crr genes of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system: a complex operon with several modes of transcription.
De Reuse H, Danchin A.
(
2457575
)
|
J Mol Biol. 1992;226(3):623-35.
Positive regulation of the expression of the Escherichia coli pts operon. Identification of the regulatory regions.
De Reuse H, Kolb A, Danchin A.
(
1324322
)
|
Gene. 1985;35(1-2):199-207.
Analysis of the ptsH-ptsI-crr region in Escherichia coli K-12: nucleotide sequence of the ptsH gene.
De Reuse H, Roy AM, Danchin A.
(
2411636
)
|
Gene. 1984;32(1-2):31-40.
Analysis of the ptsH-ptsI-crr region in Escherichia coli K-12: evidence for the existence of a single transcriptional unit.
De Reuse H, Huttner E, Danchin A.
(
6099314
)
|
| Sequences |
Amino acid
FASTA format
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0001 MISGILASPG IAFGKALLLK EDEIVIDRKK ISADQVDQEV ERFLSGRAKA SAQLETIKTK AGETFGEEKE
0071 AIFEGHIMLL EDEELEQEII ALIKDKHMTA DAAAHEVIEG QASALEELDD EYLKERAADV RDIGKRLLRN
0141 ILGLKIIDLS AIQDEVILVA ADLTPSETAQ LNLKKVLGFI TDAGGRTSHT SIMARSLELP AIVGTGSVTS
0211 QVKNDDYLIL DAVNNQVYVN PTNEVIDKMR AVQEQVASEK AELAKLKDLP AITLDGHQVE VCANIGTVRD
0281 VEGAERNGAE GVGLYRTEFL FMDRDALPTE EEQFAAYKAV AEACGSQAVI VRTMDIGGDK ELPYMNFPKE
0351 ENPFLGWRAI RIAMDRREIL RDQLRAILRA SAFGKLRIMF PMIISVEEVR ALRKEIEIYK QELRDEGKAF
0421 DESIEIGVMV ETPAAATIAR HLAKEVDFFS IGTNDLTQYT LAVDRGNDMI SHLYQPMSPS VLNLIKQVID
0491 ASHAEGKWTG MCGELAGDER ATLLLLGMGL DEFSMSAISI PRIKKIIRNT NFEDAKVLAE QALAQPTTDE
0561 LMTLVNKFIE EKTIC
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 AAGACGAGCA GAAAGCGGTT GAACATCTGG
-070 TTAAACTGAT GGCGGAACTC GAGTAATTTC CCGGGTTCTT TTAAAAATCA GTCACAAGTA AGGTAGGGTT
0001 atgatttcag gcattttagc atccccgggt atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa
0071 ttgtcattga ccggaaaaaa atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg
0141 tgccaaggca tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
0211 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata gccctgatta
0281 aagataagca catgacagct gacgcagctg ctcatgaagt tatcgaaggt caggcttctg ccctggaaga
0351 gctggatgat gaatacctga aagaacgtgc ggctgacgta cgtgatatcg gtaagcgcct gctgcgcaac
0421 atcctgggcc tgaagattat cgacctgagc gccattcagg atgaagtcat tctggttgcc gctgacctga
0491 cgccgtccga aaccgcacag ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac
0561 ttcccacacc tctatcatgg cgcgttctct ggaactacct gctatcgtgg gtaccggtag cgtcacctct
0631 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg
0701 aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg ctaaactgaa
0771 agatctgcca gctattacgc tggacggtca ccaggtagaa gtatgcgcta acattggtac ggttcgtgac
0841 gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc
0911 gcgacgcact gcccactgaa gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca
0981 agcggttatc gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
1051 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtag agagatcctg cgcgatcagc
1121 tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc ccgatgatca tctctgttga
1191 agaagtgcgt gcactgcgca aagagatcga aatctacaaa caggaactgc gcgacgaagg taaagcgttt
1261 gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg ctgccgcaac aattgcacgt catttagcca
1331 aagaagttga tttctttagt atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa
1401 tgatatgatt tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
1471 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt gctacacttc
1541 tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc ccgcgcatta agaagattat
1611 ccgtaacacg aacttcgaag atgcgaaggt gttagcagag caggctcttg ctcaaccgac aacggacgag
1681 ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa tctgctaaTC CACGAGATGC GGCCCAATTT
1751 ACTGCTTAGG AGAAGATCAT GGGTTTGTTC GATAAACTGA AATCTCTGGT TTCCGACGAC AAGAAGGATA
1821 CCGGAACT
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