|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
J Biochem (Tokyo). 1994;116(4):916-22.
Identification and characterization of the ackA (acetate kinase A)-pta (phosphotransacetylase) operon and complementation analysis of acetate utilization by an ackA-pta deletion mutant of Escherichia coli.
Kakuda H, Hosono K, Shiroishi K, Ichihara S.
(
7883769
)
|
|
|
Deletion
|
OCL65
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
ackA
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
ackA
Search strains by all related name:
ackA b2296 ECK2290 JW2293
|
General information (Go to Linear View: )
|
|
Gene Name
|
ackA
|
|
Alternative name
|
b2296,ECK2290,JW2293
|
|
Location, Length
|
2,411,492 - 2,412,694
(
+
)
;
51.98
min
;
1203
(bp) ,
400
(aa)
|
|
Product
|
acetate kinase A and propionate kinase 2
|
|
Operon Name
|
ackA-pta
|
|
Note
|
acetate kinase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0009063 - cellular amino acid catabolic process; GO_process: GO:0042867 - pyruvate catabolic process; GO_process: GO:0045733 - acetate catabolic process
|
|
Function
|
enzyme; Energy metabolism, carbon: Electron transport
|
|
Gene Ontology
|
GO:0005622
;
intracellular ( ackA )
GO:0005624
;
membrane fraction ( ackA )
GO:0005737
;
cytoplasm ( ackA )
GO:0006082
;
organic acid metabolic process ( ackA )
GO:0008152
;
metabolic process ( ackA )
GO:0008776
;
acetate kinase activity ( ackA )
GO:0016301
;
kinase activity ( ackA )
GO:0016310
;
phosphorylation ( ackA )
GO:0016740
;
transferase activity ( ackA )
GO:0016774
;
phosphotransferase activity, carboxyl group as acceptor ( ackA )
|
|
PID
|
1788633
|
EC number
(KEGG Pathway)
|
2.7.2.-
2.7.2.1
|
|
SWISS-PROT
(
Show details
)
|
Entry name(Acc.no)
|
ACKA_ECOLI
(
P15046
;
P78188; Q59386
)
|
|
- Protein name
|
Acetate kinase
|
|
- Synonyms
|
EC 2.7.2.1, Acetokinase
|
|
- Gene name
|
OrderedLocusNames=b2296, c2838, z3558, ECs3180;
|
|
MMBR References
|
J Gen Microbiol. 1979;115(2):259-71.
Anaerobic growth of Escherichia coli K12 with fumarate as terminal electron acceptor. Genetic studies with menaquinone and fluoroacetate-resistant mutants.
Guest JR.
(
393800
)
|
J. Biochem. (Tokyo) 1994;116:916-922
Identification and characterization of the ackA (acetate kinase A)-pta (phosphotransacetylase) operon and complementation analysis of acetate utilization by an ackA-pta deletion mutant of Escherichia coli.
Kakuda, H., K. Hosono, K. Shiroishi, and S. Ichihara.
|
J Bacteriol. 1998;180:985-988
Both acetate kinase and acetyl coenzyme A synthetase are involved in acetate-stimulated change in the direction of flagellar rotation in Escherichia coli.
Barak, R., W. N. Abouhamad, and M. Eisenbach.
|
J Bacteriol. 1980;143(2):1081-5.
Isolation and Characterization of acetate kinase and phosphotransacetylase mutants of Escherichia coli and Salmonella typhimurium.
LeVine SM, Ardeshir F, Ames GF.
(
6259116
)
|
J Bacteriol. 1989;171(1):577-80.
Cloning, expression, and nucleotide sequence of the Escherichia coli K-12 ackA gene.
Matsuyama A, Yamamoto H, Nakano E.
(
2536666
)
|
J Gen Microbiol. 1977;102(2):327-36.
The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli.
Brown TD, Jones-Mortimer MC, Kornberg HL.
(
21941
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSSKLVLVLN CGSSSLKFAI IDAVNGEEYL SGLAECFHLP EARIKWKMDG NKQEAALGAG AAHSEALNFI
0071 VNTILAQKPE LSAQLTAIGH RIVHGGEKYT SSVVIDESVI QGIKDAASFA PLHNPAHLIG IEEALKSFPQ
0141 LKDKNVAVFD TAFHQTMPEE SYLYALPYNL YKEHGIRRYG AHGTSHFYVT QEAAKMLNKP VEELNIITCH
0211 LGNGGSVSAI RNGKCVDTSM GLTPLEGLVM GTRSGDIDPA IIFHLHDTLG MSVDAINKLL TKESGLLGLT
0281 EVTSDCRYVE DNYATKEDAK RAMDVYCHRL AKYIGAYTAL MDGRLDAVVF TGGIGENAAM VRELSLGKLG
0351 VLGFEVDHER NLAARFGKSG FINKEGTRPA VVIPTNEELV IAQDASRLTA
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 TGCAGTGCAT GATGTTAATC ATAAATGTCG
-070 GTGTCATCAT GCGCTACGCT CTATGGCTCC CTGACGTTTT TTTAGCCACG TATCAATTAT AGGTACTTCC
0001 atgtcgagta agttagtact ggttctgaac tgcggtagtt cttcactgaa atttgccatc atcgatgcag
0071 taaatggtga agagtacctt tctggtttag ccgaatgttt ccacctgccc gaagcacgta tcaaatggaa
0141 aatggacggc aataaacagg aagcggcttt aggtgcaggc gccgctcaca gcgaagcgct caactttatc
0211 gttaatacta ttctggcaca aaaaccagaa ctgtctgcgc agctgactgc tatcggtcac cgtatcgtac
0281 acggcggcga aaagtatacc agctccgtag tgatcgatga gtctgttatt cagggtatca aagatgcagc
0351 ttcttttgca ccgctgcaca acccggctca cctgatcggt atcgaagaag ctctgaaatc tttcccacag
0421 ctgaaagaca aaaacgttgc tgtatttgac accgcgttcc accagactat gccggaagag tcttacctct
0491 acgccctgcc ttacaacctg tacaaagagc acggcatccg tcgttacggc gcgcacggca ccagccactt
0561 ctatgtaacc caggaagcgg caaaaatgct gaacaaaccg gtagaagaac tgaacatcat cacctgccac
0631 ctgggcaacg gtggttccgt ttctgctatc cgcaacggta aatgcgttga cacctctatg ggcctgaccc
0701 cgctggaagg tctggtcatg ggtacccgtt ctggtgatat cgatccggcg atcatcttcc acctgcacga
0771 caccctgggc atgagcgttg acgcaatcaa caaactgctg accaaagagt ctggcctgct gggtctgacc
0841 gaagtgacca gcgactgccg ctatgttgaa gacaactacg cgacgaaaga agacgcgaag cgcgcaatgg
0911 acgtttactg ccaccgcctg gcgaaataca tcggtgccta cactgcgctg atggatggtc gtctggacgc
0981 tgttgtattc actggtggta tcggtgaaaa tgccgcaatg gttcgtgaac tgtctctggg caaactgggc
1051 gtgctgggct ttgaagttga tcatgaacgc aacctggctg cacgtttcgg caaatctggt ttcatcaaca
1121 aagaaggtac ccgtcctgcg gtggttatcc caaccaacga agaactggtt atcgcgcaag acgcgagccg
1191 cctgactgcc tgaTTTCACA CCGCCAGCTC AGCTGGCGGT GCTGTTTTGT AACCCGCCAA ATCGGCGGTA
1261 ACGAAAGAGG ATAAACCGTG TCCCGTATTA TTATGCTGAT CCC
|
