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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Mol Gen Genet. 1978;167(2):129-37.
Lambda transducing phages for the nalA gene of Escherichia coli and conditional lethal nalA mutations.
Kreuzer KN, McEntee K, Geballe AP, Cozzarelli NR.
(
366382
)
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Deletion
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OCL68,69-10 (BK9-6 / AK7-6)
(D)
,
OCL68,69-9 (BK9-6 / AK6-6)
(D)
,
OCL69,70-1
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
gyrA
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Related strains
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Strains Search
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Search strains by gene name:
gyrA
Search strains by all related name:
gyrA b2231 ECK2223 hisW JW2225 nalA nfxA norA parD
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General information (Go to Linear View: )
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Gene Name
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gyrA
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Alternative name
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b2231,ECK2223,hisW,JW2225,nalA,nfxA,norA,parD
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Location, Length
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2,334,815 - 2,337,442
(
-
)
;
50.32
min
;
2628
(bp) ,
875
(aa)
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Product
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DNA gyrase (type II topoisomerase), subunit A
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Operon Name
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gyrA
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Note
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DNA gyrase, subunit A, type II topoisomerase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication; GO_process: GO:0006350 - transcription
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Function
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enzyme; DNA - replication, repair,restriction/modification
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Gene Ontology
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GO:0003677
;
DNA binding ( gyrA )
GO:0003916
;
DNA topoisomerase activity ( gyrA )
GO:0003918
;
DNA topoisomerase (ATP-hydrolyzing) activity ( gyrA )
GO:0005524
;
ATP binding ( gyrA )
GO:0005624
;
membrane fraction ( gyrA )
GO:0005694
;
chromosome ( gyrA )
GO:0005737
;
cytoplasm ( gyrA )
GO:0006259
;
DNA metabolic process ( gyrA )
GO:0006265
;
DNA topological change ( gyrA )
GO:0006268
;
DNA unwinding during replication ( gyrA )
GO:0016853
;
isomerase activity ( gyrA )
GO:0046677
;
response to antibiotic ( gyrA )
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PID
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1788562
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EC number
(KEGG Pathway)
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5.99.1.3
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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gyrA
(
EG10423
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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GYRA_ECOLI
(
P09097
)
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- Protein name
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DNA gyrase subunit A
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- Synonyms
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EC 5.99.1.3
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- Gene name
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OrderedLocusNames=b2231, SF2311, S2444;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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gyrA
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Structure Of The Full-Length E. Coli Parc Subunit
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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DNA gyrase subunit A
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PSI-BLAST
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Myosin-4
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nr
(database updated :
2007.02.20
)
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BLAST
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DNA gyrase subunit A [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AMIDATION
RGD
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MMBR References
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Cell. 1982;31(1):43-51.
Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes.
DiNardo S, Voelkel KA, Sternglanz R, Reynolds AE, Wright A.
(
6297752
)
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J Bacteriol. 1969;99(1):238-41.
Escherichia coli K-12 mutants resistant to nalidixic acid: genetic mapping and dominance studies.
Hane MW, Wood TH.
(
4895844
)
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Antimicrob Agents Chemother. 1996;40:879-885
Genetic evidence for a role of parC mutations in development of high-level fluoroquinolone resistance in Escherichia coli.
Heisig, P.
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Mol Microbiol. 1987;1(3):259-73.
The parD- mutant of Escherichia coli also carries a gyrAam mutation. The complete sequence of gyrA.
Hussain K, Elliott EJ, Salmond GP.
(
2834621
)
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J Bacteriol. 1992;174:5798-5802
DNA gyrase, CS7.4, and the cold shock response in Escherichia coli.
Jones, P. G., R. Krah, S. R. Tafuri, and A. P. Wolffe.
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J Biol Chem. 1996;271:24806-24810
Heat shock-induced DNA relaxation in vitro by DNA gyrase of Escherichia coli in the presence of ATP.
Kataoka, K., T. Mizushima, Y. Ogata, T. Miki, and K. Sekimizu.
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J Bacteriol. 1979;140(2):424-35.
Escherichia coli mutants thermosensitive for deoxyribonucleic acid gyrase subunit A: effects on deoxyribonucleic acid replication, transcription, and bacteriophage growth.
Kreuzer KN, Cozzarelli NR.
(
227840
)
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J Bacteriol. 1987;169(3):1272-8.
Fusions of the Escherichia coli gyrA and gyrB control regions to the galactokinase gene are inducible by coumermycin treatment.
Menzel R, Gellert M.
(
3029031
)
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Proc Natl Acad Sci U S A. 1987;84:4185-4189
Modulation of transcription by DNA supercoiling: a deletion analysis of the Escherichia coli gyrA and gyrB promoters.
Menzel, R., and M. Gellert.
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Antimicrob Agents Chemother. 1989;33(2):254-5.
gyrA and gyrB mutations in quinolone-resistant strains of Escherichia coli.
Nakamura S, Nakamura M, Kojima T, Yoshida H.
(
2655532
)
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J. Biochem. (Tokyo) 1976;62:491-497.
Replication of Escherichia coli DNA in vitro: inhibition by oxoline acid.
Staudenbauer, W. L.
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J Bacteriol. 1994;176:5999-6006
Mutations in the 10 TATAAT sequence of the gyrA promoter affect both promoter strength and sensitivity to DNA supercoiling.
Straney, R., R. Krah, and R. Menzel.
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J Mol Biol. 1987;197(4):729-36.
Cloning and sequencing of the Escherichia coli gyrA gene coding for the A subunit of DNA gyrase.
Swanberg SL, Wang JC.
(
2828631
)
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Antimicrob Agents Chemother. 1997;41:85-90
A novel, double mutation in DNA gyrase A of Escherichia coli conferring resistance to quinolone antibiotics.
Truong, Q. C., J. C. Nguyen Van, D. Shlaes, L. Gutmann, and N. J. Moreau.
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J Bacteriol. 1990;172(12):6617
Location of gyrA on the physical map of the Escherichia coli chromosome.
Webb G, Rohatgi K, Courtright JB.
(
2254240
)
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Microbiol Immunol. 1995;39(4):243-7.
Analysis of the NH2-terminal 83rd amino acid of Escherichia coli GyrA in quinolone-resistance.
Yonezawa M, Takahata M, Banzawa N, Matsubara N, Watanabe Y, Narita H.
(
7651238
)
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Mol Gen Genet. 1988;211(1):1-7.
Quinolone-resistant mutations of the gyrA gene of Escherichia coli.
Yoshida H, Kojima T, Yamagishi J, Nakamura S.
(
2830458
)
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| Sequences |
Amino acid
FASTA format
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0001 MSDLAREITP VNIEEELKSS YLDYAMSVIV GRALPDVRDG LKPVHRRVLY AMNVLGNDWN KAYKKSARVV
0071 GDVIGKYHPH GDSAVYDTIV RMAQPFSLRY MLVDGQGNFG SIDGDSAAAM RYTEIRLAKI AHELMADLEK
0141 ETVDFVDNYD GTEKIPDVMP TKIPNLLVNG SSGIAVGMAT NIPPHNLTEV INGCLAYIDD EDISIEGLME
0211 HIPGPDFPTA AIINGRRGIE EAYRTGRGKV YIRARAEVEV DAKTGRETII VHEIPYQVNK ARLIEKIAEL
0281 VKEKRVEGIS ALRDESDKDG MRIVIEVKRD AVGEVVLNNL YSQTQLQVSF GINMVALHHG QPKIMNLKDI
0351 IAAFVRHRRE VVTRRTIFEL RKARDRAHIL EALAVALANI DPIIELIRHA PTPAEAKTAL VANPWQLGNV
0421 AAMLERAGDD AARPEWLEPE FGVRDGLYYL TEQQAQAILD LRLQKLTGLE HEKLLDEYKE LLDQIAELLR
0491 ILGSADRLME VIREELELVR EQFGDKRRTE ITANSADINL EDLITQEDVV VTLSHQGYVK YQPLSEYEAQ
0561 RRGGKGKSAA RIKEEDFIDR LLVANTHDHI LCFSSRGRVY SMKVYQLPEA TRGARGRPIV NLLPLEQDER
0631 ITAILPVTEF EEGVKVFMAT ANGTVKKTVL TEFNRLRTAG KVAIKLVDGD ELIGVDLTSG EDEVMLFSAE
0701 GKVVRFKESS VRAMGCNTTG VRGIRLGEGD KVVSLIVPRG DGAILTATQN GYGKRTAVAE YPTKSRATKG
0771 VISIKVTERN GLVVGAVQVD DCDQIMMITD AGTLVRTRVS EISIVGRNTQ GVILIRTAED ENVVGLQRVA
0841 EPVDEEDLDT IDGSAAEGDD EIAPEVDVDD EPEEE
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 attggatgtg aataaagcgt ataggtttac
-070 ctcaaactgc gcggctgtgt tataatttgc gacctttgaa tccgggatac agtagaggga tagcggttag
0001 atgagcgacc ttgcgagaga aattacaccg gtcaacattg aggaagagct gaagagctcc tatctggatt
0071 atgcgatgtc ggtcattgtt ggccgtgcgc tgccagatgt ccgagatggc ctgaagccgg tacaccgtcg
0141 cgtactttac gccatgaacg tactaggcaa tgactggaac aaagcctata aaaaatctgc ccgtgtcgtt
0211 ggtgacgtaa tcggtaaata ccatccccat ggtgactcgg cggtctatga cacgatcgtc cgcatggcgc
0281 agccattctc gctgcgttat atgctggtag acggtcaggg taacttcggt tctatcgacg gcgactctgc
0351 ggcggcaatg cgttatacgg aaatccgtct ggcgaaaatt gcccatgaac tgatggccga tctcgaaaaa
0421 gagacggtcg atttcgttga taactatgac ggcacggaaa aaattccgga cgtcatgcca accaaaattc
0491 ctaacctgct ggtgaacggt tcttccggta tcgccgtagg tatggcaacc aacatcccgc cgcacaacct
0561 gacggaagtc atcaacggtt gtctggcgta tattgatgat gaagacatca gcattgaagg gctgatggaa
0631 cacatcccgg ggccggactt cccgacggcg gcaatcatta acggtcgtcg cggtattgaa gaagcttacc
0701 gtaccggtcg cggcaaggtg tatatccgcg ctcgcgcaga agtggaagtt gacgccaaaa ccggtcgtga
0771 aaccattatc gtccacgaaa ttccgtatca ggtaaacaaa gcgcgcctga tcgagaagat tgcggaactg
0841 gtaaaagaaa aacgcgtgga aggcatcagc gcgctgcgtg acgagtctga caaagacggt atgcgcatcg
0911 tgattgaagt gaaacgcgat gcggtcggtg aagttgtgct caacaacctc tactcccaga cccagttgca
0981 ggtttctttc ggtatcaaca tggtggcatt gcaccatggt cagccgaaga tcatgaacct gaaagacatc
1051 atcgcggcgt ttgttcgtca ccgccgtgaa gtggtgaccc gtcgtactat tttcgaactg cgtaaagctc
1121 gcgatcgtgc tcatatcctt gaagcattag ccgtggcgct ggcgaacatc gacccgatca tcgaactgat
1191 ccgtcatgcg ccgacgcctg cagaagcgaa aactgcgctg gttgctaatc cgtggcagct gggcaacgtt
1261 gccgcgatgc tcgaacgtgc tggcgacgat gctgcgcgtc cggaatggct ggagccagag ttcggcgtgc
1331 gtgatggtct gtactacctg accgaacagc aagctcaggc gattctggat ctgcgtttgc agaaactgac
1401 cggtcttgag cacgaaaaac tgctcgacga atacaaagag ctgctggatc agatcgcgga actgttgcgt
1471 attcttggta gcgccgatcg tctgatggaa gtgatccgtg aagagctgga gctggttcgt gaacagttcg
1541 gtgacaaacg tcgtactgaa atcaccgcca acagcgcaga catcaacctg gaagatctga tcacccagga
1611 agatgtggtc gtgacgctct ctcaccaggg ctacgttaag tatcagccgc tttctgaata cgaagcgcag
1681 cgtcgtggcg ggaaaggtaa atctgccgca cgtattaaag aagaagactt tatcgaccga ctgctggtgg
1751 cgaacactca cgaccatatt ctgtgcttct ccagccgtgg tcgcgtctat tcgatgaaag tttatcagtt
1821 gccggaagcc actcgtggcg cgcgcggtcg tccgatcgtc aacctgctgc cgctggagca ggacgaacgt
1891 atcactgcga tcctgccagt gaccgagttt gaagaaggcg tgaaagtctt catggcgacc gctaacggta
1961 ccgtgaagaa aactgtcctc accgagttca accgtctgcg taccgccggt aaagtggcga tcaaactggt
2031 tgacggcgat gagctgatcg gcgttgacct gaccagcggc gaagacgaag taatgctgtt ctccgctgaa
2101 ggtaaagtgg tgcgctttaa agagtcttct gtccgtgcga tgggctgcaa caccaccggt gttcgcggta
2171 ttcgcttagg tgaaggcgat aaagtcgtct ctctgatcgt gcctcgtggc gatggcgcaa tcctcaccgc
2241 aacgcaaaac ggttacggta aacgtaccgc agtggcggaa tacccaacca agtcgcgtgc gacgaaaggg
2311 gttatctcca tcaaggttac cgaacgtaac ggtttagttg ttggcgcggt acaggtagat gactgcgacc
2381 agatcatgat gatcaccgat gccggtacgc tggtacgtac tcgcgtttcg gaaatcagca tcgtgggccg
2451 taacacccag ggcgtgatcc tcatccgtac tgcggaagat gaaaacgtag tgggtctgca acgtgttgct
2521 gaaccggttg acgaggaaga tctggatacc atcgacggca gtgccgcgga aggggacgat gaaatcgctc
2591 cggaagtgga cgttgacgac gagccagaag aagaataatt ttacttcttc atgccaaaag ggagctatct
2661 cccttgtttg aattgaaaag tccaggctgc aaagtctggg cttttgtcgt attagggcac ggtaaagt
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