MG1655
W3110
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Gene Report : rnhA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR36 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  rnhA
Related strains
     Strains Search Search strains by gene name:  rnhA   Search strains by all related name:  rnhA b0214 cer dasF ECK0214 herA JW0204 rnh sdrA sin

General information  (Go to Linear View:)
 Gene Name rnhA  
 Alternative name b0214,cer,dasF,ECK0214,herA,JW0204,rnh,sdrA,sin  
 Location, Length 235,535 - 236,002 (  -  ) ;   5.08 min ; 468 (bp) ,   155 (aa) Go to Linear View
 Product ribonuclease HI, degrades RNA of DNA-RNA hybrids  
 Operon Name rnhA  
 Note RNase HI, degrades RNA of DNA-RNA hybrids, participates in DNA replication; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006401 - RNA catabolic process; GO_process: GO:0006261 - DNA-dependent DNA replication; GO_process: GO:0006508 - proteolysis  
 Function enzyme; Degradation of RNA  
 Gene Ontology GO:0000287 ; magnesium ion binding ( rnhA )
GO:0003676 ; nucleic acid binding ( rnhA )
GO:0004518 ; nuclease activity ( rnhA )
GO:0004519 ; endonuclease activity ( rnhA )
GO:0004523 ; ribonuclease H activity ( rnhA )
GO:0005737 ; cytoplasm ( rnhA )
GO:0006401 ; RNA catabolic process ( rnhA )
GO:0016787 ; hydrolase activity ( rnhA )
GO:0046872 ; metal ion binding ( rnhA )
 PID 1786408  
 EC number
  (KEGG Pathway)
 
3.1.26.4  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene rnhA ( EG10860 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) RNH_ECOLI ( P00647 ; Q8FKY5 )
    -  Protein name Ribonuclease HI  
    -  Synonyms EC 3.1.26.4, RNase HI, Ribonuclease H, RNase H  
    -  Gene name OrderedLocusNames=b0214, c0251, z0239, ECs0210, SF0201, S0208;  

 Linear View (Whole Mode)
View Location
   225.0  –  250.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP rnhA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain , Ribonuclease H  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Ribonuclease HI (RNase HI) (Ribonuclease H) (RNase H)  
         PSI-BLAST Ribonuclease HI (RNase HI) (Ribonuclease H) (RNase H)
    nr     (database updated : 2007.02.20 )
         BLAST RNase HI, degrades RNA of DNA-RNA hybrids, participates in DNA replication [Escherichia coli UTI89]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE CK2_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG0328L 
    EcoCyc rnhA 

MMBR References
J Biol Chem. 1982;257:4719-4722
A mutation in the rnh-locus of Escherichia coli affects the structural gene for RNase H. Examination of the mutant and wild type protein.
Arendes, J., P. Carl, and A. Sugino.
J Bacteriol. 1993;175:6731-6734
Absence of a direct role for RNase HI in initiation of DNA replication at the oriC site on the Escherichia coli chromosome.
Hong, X., and T. Kogoma.
Proc Natl Acad Sci U S A. 1981;78:3770-3774
Identification of the dnaQ gene product and location of the structural gene for RNase H of Escherichia coli by cloning of the genes.
Horiuchi, T., H. Maki, M. Maruyana, and M. Sekiguchi.
J Biol Chem. 1983;258:1276-1281
DNA sequence of the gene coding for Escherichia coli ribonuclease H.
Kanaya, S., and R. J. Crouch.
J Bacteriol. 1994;176:1521-1523
Escherichia coli RNA polymerase mutants that enhance or diminish the SOS response constitutively expressed in the absence of RNase H1 activity.
Kogoma, T.
Biochimie. 1993;75(1-2):89-99.
Requirement of homologous recombination functions for viability of the Escherichia coli cell that lacks RNase HI and exonuclease V activities.
Kogoma T, Hong X, Cadwell GW, Barnard KG, Asai T. ( 8389213 )
Plasmid. 1995;33(3):235-8.
Isolation and characterization of host mutants defective in msDNA synthesis: role of ribonuclease H in msDNA synthesis.
Lima TM, Lim D. ( 7568472 )
Proc Natl Acad Sci U S A. 1983;80:7137-7141
Structure and expression of the dnaQ mutator and the RNase H genes of Escherichia coli: overlap of the promoter regions.
Maki, H., T. Horiuchi, and M. Sekiguchi.
Proc Natl Acad Sci U S A. 1984;81:550-554
Escherichia coli mutants suppressing replication-defective mutations of the ColE1 plasmid.
Naito, S., T. Kitani, T. Ogawa, T. Okazaki, and H. Uchida.
J Biol Chem. 1985;260:7122-7125
Transcriptional organization of the convergent overlapping dnaQ-rnh genes of Escherichia coli.
Nomura, T., H. Aiba, and A. Ishihama.
Proc Natl Acad Sci U S A. 1984;81:1040-1044
RNase H confers specificity in the dnaA-dependent initiation of replication at the unique origin of the Escherichia coli chromosome in vivo and in vitro.
Ogawa, T., G. G. Pickett, T. Kogoma, and A. Kornberg.
Mol Gen Genet. 1984;196(2):350-5.
dnaA suppressor (dasF) mutants of Escherichia coli are stable DNA replication (sdrA/rnh) mutants.
Torrey TA, Atlung T, Kogoma T. ( 6092872 )
J Bacteriol. 1993;175:7247-7253
DNA polymerase I and the bypassing of RecA dependence of constitutive stable DNA replication in Escherichia coli rnhA mutants.
Cao, Y., R. R. R. Rowland, and T. Kogoma.
J Bacteriol. 1993;175:7254-7259
Requirement for the polymerization and 5'3' exonuclease activities of DNA polymerase I in initiation of DNA replication at oriK sites in the absence of RecA in Escherichia coli rnhA mutants.
Cao, Y., and T. Kogoma.
J Bacteriol. 1980;144(1):28-35.
Isolation and mapping of a mutation in Escherichia coli with altered levels of ribonuclease H.
Carl PL, Bloom L, Crouch RJ. ( 6998952 )
J Mol Biol. 1986;190(1):113-7.
DNA sequence and coding properties of mutD(dnaQ) a dominant Escherichia coli mutator gene.
Cox EC, Horner DL. ( 3023634 )

Sequences
Amino acid
FASTA format
0001 MLKQVEIFTD GSCLGNPGPG GYGAILRYRG REKTFSAGYT RTTNNRMELM AAIVALEALK EHCEVILSTD 
0071 SQYVRQGITQ WIHNWKKRGW KTADKKPVKN VDLWQRLDAA LGQHQIKWEW VKGHAGHPEN ERCDELARAA 
0141 AMNPTLEDTG YQVEV

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             atcgagaacg atctggcgtg taattgcagt 
-070 gctcatagcg gtcatttatg tcagacttgt cgttttacag ttcgattcaa ttacaggaag tctaccagag 
0001 atgcttaaac aggtagaaat tttcaccgat ggttcgtgtc tgggcaatcc aggacctggg ggttacggcg 
0071 ctattttacg ctatcgcgga cgcgagaaaa cctttagcgc tggctacacc cgcaccacca acaaccgtat 
0141 ggagttgatg gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg aagtcatttt gagtaccgac 
0211 agccagtatg tccgccaggg tatcacccag tggatccata actggaaaaa acgtggctgg aaaaccgcag 
0281 acaaaaaacc agtaaaaaat gtcgatctct ggcaacgtct tgatgctgca ttggggcagc atcaaatcaa 
0351 atgggaatgg gttaaaggcc atgccggaca cccggaaaac gaacgctgtg atgaactggc tcgtgccgcg 
0421 gcgatgaatc ccacactgga agatacaggc taccaagttg aagtttaagc ctgtggttta cgacattgcc 
0491 gggtggctcc aaccgcctgg cgaattcgtg gcttgttttt actctgtttc atcggattta gcgttaaagg 
0561 aatagtcc